Laccases have high biotechnological potential in industries since they catalyze the oxidation of many chemical compounds. The production of laccases by fungi has been extensively studied due to their ...secretion of enzymes and rapid growth using cheap substrates.
Trichoderma;
the versatile fungal genus includes species of great biotechnological value and considered as a magnificent industrial cell factory of enzymes. In this study, 10
Trichoderma
species were screened for laccase enzyme production by submerged cultivation. The studied species were identified by internal transcribed spacer (ITS) gene sequences. Guaiacol (0.04%) as an enzyme substrate in plate medium was used for the selection of maximum laccase-enriched
Trichoderma
species by formation of visual color halo intensity. This activity was evaluated by liquid submersion (flask medium) also. The absorbance of laccase contained broth was measured by a spectrophotometer (450 nm). The highest laccase production was obtained by
T. atroviride
(2.62 U/mL).
Trichoderma cremeum
and
T. longipile
showed medium laccase potency, while
T. beinartii
exhibited weak laccase secretion ability. Laccase from
T. atroviride
was purified by SDS-PAGE and the molecular weight was determined (57 kDa). The laccase was confirmed by their respective amino acid sequences, and the phylogenetic tree was constructed for further analysis.
The aim of this work to study an efficient laccase producing fungus Ganoderma leucocontextum, which was identified by ITS regions of DNA and phylogenetic tree was constructed. This study showed the ...laccase first-time from G. leucocontextum by using medium containing guaiacol. The growth cultural (pH, temperature, incubation days, rpm) and nutritional (carbon and nitrogen sources) conditions were optimized, which enhanced the enzyme production up to 4.5-folds. Laccase production increased 855 U/L at 40 °C. The pH 5.0 was suitable for laccase secretion (2517 U/L) on the 7th day of incubation at 100 rpm (698.3 U/L). Glucose and sucrose were good carbon source to enhance the laccase synthesis. The 10 g/L beef (4671 U/L) and yeast extract (5776 U/L) were the best nitrogen source for laccase secretion from G. leucocontextum. The laccase was purified from the 80% ammonium sulphate precipitations of protein identified by nucleotides sequence. The molecular weight (65.0 kDa) of purified laccase was identified through SDS and native PAGE entitled as Glacc110. The Glacc110 was characterized under different parameters. It retained > 90% of its activity for 16 min incubation at 60 °C in acidic medium (pH 4.0). This enzyme exerted its optimal activity at pH 3.0 and temperature 70 °C with guaiacol substrate. The catalytic parameters K
and V
was 1.658 (mM) and 2.452 (mM/min), respectively. The thermo stability of the laccase produced by submerged fermentation of G. leucocontextum has potential for industrial and biotechnology applications. The results remarked the G. leucocontextum is a good source for laccase production.
The presence and increase of challenges to eHealth in today’s society have begun to generate doubts about the capability of technology in patient empowerment, especially within the frameworks ...supporting empowerment. Through the review of existing frameworks and articulation of patient demands, weaknesses in the current application of technology to support empowerment are explored, and key constituents of a technology-driven framework for patient empowerment are determined. This article argues that existing usage of technology in the design, development and implementation of patient empowerment in the healthcare system, although well intentioned, is insufficiently constituted, primarily as a result of fragmentation. Systems theory concepts such as holism and iteration are considered vital in improving the role of technology in enabling patient empowerment.
Pure water, i.e., a sign of life, continuously circulates and is contaminated by different discharges. This emerging environmental problem has been attracting the attention of scientists searching ...for methods for the treatment of wastewater contaminated by multiple recalcitrant compounds. Various physical and chemical methods are used to degrade contaminants from water bodies. Traditional methods have certain limitations and complexities for bioenergy production, which motivates the search for new ways of sustainable bioenergy production and wastewater treatment. Biological strategies have opened new avenues to the treatment of wastewater using oxidoreductase enzymes for the degradation of pollutants. Fungal-based fuel cells (FFCs), with their catalysts, have gained considerable attention among scientists worldwide. They are a new, ecofriendly, and alternative approach to nonchemical methods due to easy handling. FFCs are efficiently used in wastewater treatment and the production of electricity for power generation. This article also highlights the construction of fungal catalytic cells and the enzymatic performance of different fungal species in energy production and the treatment of wastewater.
The application of nanoparticles (NPs) has been proved as an efficient and promising technique for mitigating a wide range of stressors in plants. The present study elucidates the synergistic effect ...of iron oxide nanoparticles (IONPs) and silicon nanoparticles (SiNPs) in the attenuation of Cd toxicity in
. Seeds of
were treated with IONPs (10 mg/L) and SiNPs (20 mg/L). Seedlings of uniform size were transplanted to pots for 40 days. The results demonstrated that nanoparticles (NPs) enhanced growth, net photosynthetic rate, and gas exchange attributes in
plants grown in Cd-contaminated soil. Synergistic application of IONPs and SiNPs raised not only K
content, but also biosynthesis of polyamines (PAs), which alleviated Cd stress in
seedlings. Additionally, NPs decreased malondialdehyde (MDA) content and electrolyte leakage (EL) in
plants exposed to Cd stress. These findings suggest that stress alleviation was mainly attributed to the enhanced accumulation of K
content, improved antioxidant defense system, and higher spermidine (Spd) and putrescine (Put) levels. It is suggested that various forms of NPs can be applied synergistically to minimize heavy metal stress, thus increasing crop production under stressed conditions.
Agitation speed influenced the production rate of laccase. Orbital speed not only influenced the enzyme production, but was also effective to dissolve the oxygen during growth of mycelium, spores, ...and chlamydospores. Shear effects of speed greatly influenced the morphology of mycelium. Ganoderma multistipitatum was identified by ITS marker. Phylogenetic tree was constructed for species identification. Qualitatively by plate method contained guaiacol indicator, while quantitatively by submerged fermentation and Central Composite Design applied on agitation parameter for maximum laccase potential of this species. The effects of agitation speed on mycelium morphology were observed under compound and scanning electron microscope. Statistical optimization of agitation conditions were performed by using response surface methodology to enhance the production of laccase from Ganoderma multistipitatum sp. nov. Maximum laccase yield (19.44 x 10.sup.5 + or - 0.28 U/L) was obtained at 150 rpm grown culture, which was higher than predicted value of laccase production (19.18 x 10.sup.5 U/L) under aerobic conditions (150 rpm). The 150 rpm provided the continuous flush of oxygen. The DO (dissolved oxygen) was maximum (65%) for "27 h" incubation at 150 rpm during laccase synthesis. The statistical value of laccase production was minimum under anaerobic or nearly static condition of 50 rpm. The predicted (12.78 x 10.sup.5 U/L) and obtained (12.82 x 10.sup.5 U/L) yield was low at 50 rpm. Optimization of orbital shaking for aeration conditions were performed by the use of "Response Surface Methodology". The submerged shaking flasks were utilized as a nutrients growth medium to maximize the production of laccase from G. multistipitatum. The minimum incubation time highly influenced the laccase yield from 7 to 15 days via utilization of less cost-effective medium under a promising and eco-friendly method. The morphological effects of rpm on mycelium were examined under compound and scanning electron microscopy. Higher rpm (200, 230) shear the mycelium, while 150 to 200 rpm exhibited smoother and highly dense branches of mycelia. The shear forces of 200 rpm caused the damages of mycelium and cells autolysis with less laccase production. This study concluded that 150 rpm saved the life of mycelium and enhanced the production rate of enzymes.
Abstract
Aims
Salinity adversely affects okra
Abelmoschus esculentus
(L.) Moench plants by inducing osmotic and oxidative stresses. This study was designed to enhance salinity-induced osmotic and ...oxidative stress tolerance in okra plants by applying organic amendments.
Methods
The effects of different organic amendments (municipal solid waste compost, farmyard manure (FYM) and press mud) on osmotic potential, water use efficiency, activities of antioxidant enzymes, total soluble sugar, total soluble proline, total soluble protein and malondialdehyde (MDA) contents of okra plants grown under saline conditions (50 mM sodium chloride) were evaluated in a pot experiment. The organic amendments were applied each at the rate of 5% and 10% per pot or in various combinations (compost + FYM, FYM + press mud and compost + press mud each at the rate of 2.5% and 5% per pot).
Results
As compared to control, high total soluble sugar (60.41), total soluble proline (33.88%) and MDA (51%) contents and increased activities of antioxidant enzymes superoxide dismutase (83.54%), catalase (78.61%), peroxidase (53.57% in salinity-stressed okra plants, were indicative of oxidative stress. Salinity significantly reduced the osmotic potential (41.78%) and water use efficiency (4.75%) of okra plants compared to control. Under saline conditions, 5% (farmyard manure + press mud) was the most effective treatment, which significantly improved osmotic potential (27.05%), total soluble sugar (4.20%), total soluble protein (73.62%) and total soluble proline (23.20%) contents and superoxide dismutase activity (32.41%), compared to saline soil. Application of 2.5% (FYM + press mud), 5% press mud, and 10% compost significantly reduced MDA content (27%) and improved activities of catalase (38.64%) and peroxidase (48.29%), respectively, compared to saline soil, thus facilitated to alleviate oxidative stress in okra plants.
Conclusions
Using organic amendments (municipal solid waste compost, farmyard manure and press mud) was a cost-effective approach to improve salinity-induced osmotic and oxidative stress tolerance in okra plants.
Cotton is a major fiber crop grown worldwide. Nitrogen (N) is an essential nutrient for cotton production and supports efficient crop production. It is a crucial nutrient that is required more than ...any other. Nitrogen management is a daunting task for plants; thus, various strategies, individually and collectively, have been adopted to improve its efficacy. The negative environmental impacts of excessive N application on cotton production have become harmful to consumers and growers. The 4R’s of nutrient stewardship (right product, right rate, right time, and right place) is a newly developed agronomic practice that provides a solid foundation for achieving nitrogen use efficiency (NUE) in cotton production. Cropping systems are equally crucial for increasing production, profitability, environmental growth protection, and sustainability. This concept incorporates the right fertilizer source at the right rate, time, and place. In addition to agronomic practices, molecular approaches are equally important for improving cotton NUE. This could be achieved by increasing the efficacy of metabolic pathways at the cellular, organ, and structural levels and NUE-regulating enzymes and genes. This is a potential method to improve the role of N transporters in plants, resulting in better utilization and remobilization of N in cotton plants. Therefore, we suggest effective methods for accelerating NUE in cotton. This review aims to provide a detailed overview of agronomic and molecular approaches for improving NUE in cotton production, which benefits both the environment and growers.
Color chemicals contaminate pure water constantly discharged from different points and non-point sources. Physical and chemical techniques have certain limitations and complexities for bioenergy ...production, which motivated the search for a novel sustainable production approaches during dye wastewater treatment. The emerging environmental problem of dye decolorization has attracted scientist's attention to a new, cheap, and economical way to treat dye wastewater and power production via fungal fuel cells. Ganoderma gibbosum was fitted in the cathodic region with laccase secretion in the fuel cell. At the same time, dye water was placed in the anodic region to move electrons and produce power. This study treated wastewater using the oxidoreductase enzymes released extracellularly from Ganoderma gibbosum for dye Remazol Brilliant Blue R (RBBR) degradation via fungal-based fuel cell. The maximum power density of 14.18 mW/m
and the maximum current density of 35 mA/m
were shown by the concentration of 5 ppm during maximum laccase activity and decolorization of RBBR. The laccase catalysts have gained considerable attention because of eco-friendly and alternative easy handling approaches to chemical methods. Fungal Fuel Cells (FFCs) are efficiently used in dye treatment and electricity production. This article also highlighted the construction of fungal catalytic cells and the enzymatic performance of fungal species in energy production during dye water treatment.
Integrating fungi into fuel cell systems presents a promising opportunity to address environmental pollution while simultaneously generating energy. This review explores the innovative concept of ...constructing wetlands as fuel cells for pollutant degradation, offering a practical and eco-friendly solution to pollution challenges. Fungi possess unique capabilities in producing power, fuel, and electricity through metabolic processes, drawing significant interest for applications in remediation and degradation. Limited data exist on fungi's ability to generate electricity during catalytic reactions involving various enzymes, especially while remediating pollutants. Certain species, such as
spp.,
, and
, have been reported to generate electricity at 1200 mW/m
, 207 mW/m
, 1,163 mW/m
, 438 mW/m
, 850,000 mW/m
, and 2,900 mW/m
, respectively. Despite the eco-friendly potential compared to conventional methods, fungi's role remains largely unexplored. This review delves into fungi's exceptional potential as fuel cell catalysts, serving as anodic or cathodic agents to mitigate land, air, and water pollutants while simultaneously producing fuel and power. Applications cover a wide range of tasks, and the innovative concept of wetlands designed as fuel cells for pollutant degradation is discussed. Cost-effectiveness may vary depending on specific contexts and applications. Fungal fuel cells (FFCs) offer a versatile and innovative solution to global challenges, addressing the increasing demand for alternative bioenergy production amid population growth and expanding industrial activities. The mechanistic approach of fungal enzymes via microbial combinations and electrochemical fungal systems facilitates the oxidation of organic substrates, oxygen reduction, and ion exchange membrane orchestration of essential reactions. Fungal laccase plays a crucial role in pollutant removal and monitoring environmental contaminants. Fungal consortiums show remarkable potential in fine-tuning FFC performance, impacting both power generation and pollutant degradation. Beyond energy generation, fungal cells effectively remove pollutants. Overall, FFCs present a promising avenue to address energy needs and mitigate pollutants simultaneously.
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