Transformerless grid-connected inverters offer greater efficiencies when transferring power from renewable energy sources to the electrical grid. If the grid-inverter connection is done with an LCL ...filter, high attenuation of switching harmonics is achieved while preserving a small-size output filter. However, damping must be included in the controller to assure closed-loop stability. This paper proposes a reference computation methodology for the inverter-side current feedback in a photovoltaic (PV) generation system connected to the grid through an LCL filter. Theoretical analysis of the closed-loop system stability and of the steady-state performance are presented as well as experimental validation of the closed-loop performance. The feedback controller includes active damping and relies on a resonant control structure which improves the ability of dealing with grid harmonic distortion. The controller uses a reduced set of measurements, which requires the inverter-side current and grid voltage only, and assures a power factor close to unity.
This paper presents the converter design of a single-phase non-isolated step-down controlled rectifier for power factor improvement and output voltage regulation. The converter consists of a ...full-bridge diode rectifier and a DC-DC interleaved buck converter of two or more switching cells that has an LC filter in its input. It is proposed that the interleaved switching cells operate in discontinuous conduction mode and the current through the input LC filter be continuous, avoiding switching frequency components to be injected into the grid. The controller, which has a simple structure and a small number of sensors, allows the system to achieve a high power factor. It also regulates the output voltage to a constant reference. An experimental prototype is built and tested to validate the analysis and proposed design. The closed-loop converter is evaluated both in a steady state and in transient conditions. At steady state, the converter achieves a power factor above 0.9 with a maximum of 45.4% THD at 110.1W. The main contributions of this paper are guidelines for the design of the converter, open-loop analysis, and converter control.
Cellular mechanical properties constitute good markers to characterize tumor cells, to study cell population heterogeneity and to highlight the effect of drug treatments. In this work, we describe ...the fabrication and validation of an integrated optofluidic chip capable of analyzing cellular deformability on the basis of the pressure gradient needed to push a cell through a narrow constriction. We demonstrate the ability of the chip to discriminate between tumorigenic and metastatic breast cancer cells (MCF7 and MDA-MB231) and between human melanoma cells with different metastatic potential (A375P and A375MC2). Moreover, we show that this chip allows highlighting the effect of drugs interfering with microtubule organization (paclitaxel, combretastatin A-4 and nocodazole) on cancer cells, which leads to changes in the pressure-gradient required to push cells through the constriction. Our single-cell microfluidic device for mechanical evaluation is compact and easy to use, allowing for an extensive use in different laboratory environments.
This work presents the design of a control law based on the average model of a shunt active power filter considering an H-bridge neutral point clamped topology and its experimental validation. ...Therefore, the proposed controller is formed by three control loops, namely current (inner), regulation (outer), and balance control loops. The current loop aims to compensate both the displacement power factor and the harmonic distortion produced by nonlinear loads connected to the point of common coupling. To deal with harmonic current distortion, the current loop involves an adaptive mechanism based on a bank of resonant filters tuned at odd harmonics of the fundamental grid frequency. The regulation and balance loops are aimed to maintain the voltage of the capacitors forming the DC-link at a desired constant level. For this, proportional-integral controllers are designed. The design of all three loops considers the average model of the system. The performance of the proposed multi-loop control law is evaluated through numerical results and real-time experimental implementation, both considering a 2 kW academic benchmark with a constant switching frequency of 7 kHz. In order to provide harmonic distortion, a nonlinear load based on an uncontrolled diode bridge rectifier is considered. Additionally, step-load changes from 0.5 kW to 1 kW are considered for the nonlinear load. As a result, a suitable current tracking, voltage regulation, and balance are observed despite parametric uncertainties, load variations, and harmonic distortion. As a consequence, in steady state, simulation results indicate that the compensated grid current THD is 1.75%; meanwhile, the nonlinear load current THD is 52.5%. Experimental results indicate that the compensated grid current THD is 2.32%; meanwhile, the nonlinear load current THD is 53.8%.
The nuclear protein high-mobility group box 1 (HMGB1) can be passively released by necrotic cells or secreted actively by several cell types to regulate immune and inflammatory responses, as well as ...tissue remodeling. We herein aimed to characterize the effect of insulin resistance on HMGB1 in adipose tissue and to examine its potential role as a metabolic regulator in β-pancreatic cells.
Plasma HMGB1 concentration and adipose HMGB1 expression were assessed in relation to obesity and insulin resistance. Cultured adipocytes from lean and obese patients were used to investigate the intracellular distribution and factors regulating HMGB1 release, as well as to test its effects on adipogenesis and lipid metabolism. A regulatory role for HMGB1 in insulin secretion was also investigated.
Circulating HMGB1 was positively associated with body mass index, while adipose HMGB1 mRNA levels correlated with the expression of inflammatory markers. Insulin resistance modified the intracellular distribution of HMGB1 in human adipocytes, with HMGB1 being predominantly nuclear in lean and obese normoglycemic individuals while localized to the cytosol in obese patients with type 2 diabetes. Adipocytes from lean individuals exposed to conditioned media from lipopolysaccharide-stimulated macrophages induced HMGB1 redistribution to the cytoplasm and release. HMGB1 treatment had no effect on differentiation and lipid metabolism in adipocytes. However, HMGB1, whose circulating levels correlated with postload insulin concentration, increased both insulin release and intracellular Ca(2+) concentration in INS-1 cells.
These findings show, for the first time, that HMGB1 expression and release by human adipocytes is altered by inflammatory conditions as those imposed by obesity and insulin resistance. Our data reveal a novel role for HMGB1 as a stimulatory factor of insulin secretion of β-pancreatic cells.
Pulse Width Modulation (PWM) strategies are crucial for controlling DC-AC power converters. In particular, transformerless inverters require specific PWM techniques to improve efficiency and to deal ...with leakage ground current issues. In this paper, three hybrid PWM methods are proposed for a DCM-232 three-phase topology. These methods are based on the concepts of carrier-based PWM and space vector modulation. Calculations of time intervals for active and null vectors are performed in a conventional way, and the resulting waveforms are compared with a carrier signal. The digital signals obtained are processed using Boolean functions, generating ten signals to control the DCM-232 three-phase inverter. The performance of the three proposed PWM methods is evaluated considering the reduction in leakage ground current and efficiency. The proposed modulation techniques have relevant performances complying with international standards, which make them suitable for transformerless three-phase photovoltaic (PV) inverter markets. To validate the proposed hybrid PWM strategies, numerical simulations and experimental tests were performed.
The application of scientific tools to analyse the use of Internet-based e-learning tools in academic settings is in general an ignored area. E-learning tools are actually an emergent topic as a ...result of the new ideas introduced by the European Higher Education Area. Lifelong learning, or the promotion of student initiative, is the new paradigm of a learner-centred education. In this context, e-learning tools can represent an effective way of supporting this new trend in education. Assuming the premise that successful use of these web-based tools depends primarily on a user's behaviour, the objective of this research is to examine the technology acceptance model (TAM) of web-based e-learning tools used in practical and laboratory teaching. The research hypotheses derived from this model have empirically been validated using the responses to a survey on e-learning usage among 220 users. These responses have been examined through partial least square. The obtained results strongly support the extended TAM in predicting a student's intention to use e-learning and define a set of external variables with a significant influence in the original TAM variables. Surprisingly, perceived ease of use did not posit a significant impact on student attitude or intention towards e-learning tool usage. Therefore, early evaluation of e-learning material is considered essential to providing a framework for further improvements of the tool. (Contains 2 figures, 3 tables, and 1 note.)
The RNA world of human ageing Gomez-Verjan, J. C.; Vazquez-Martinez, E. R.; Rivero-Segura, N. A. ...
Human genetics,
12/2018, Letnik:
137, Številka:
11-12
Journal Article
Recenzirano
Ageing is one of the most complex processes in nature; how could we prevent the associated biological changes and chronic diseases that string along with this process, is a challenge in healthcare ...around the world. Recent advances in next-generation sequencing have reached a stage where it is possible to know from a specific tissue the most abundant transcripts, alternative splicing process and, non-coding RNA molecules (microRNA’s, long non-coding RNA’s, and circular RNAs). Moreover, our knowledge of several biological processes related to ageing such as senescence and autophagy have dramatically increased in the last years. In the present review, we attempt to summarise the latest scientific advances from the most critical studies performed in human clinical samples, specific to the RNA studies about ageing. Overall, human transcriptomics research indicates that although there are common alterations of the regular expression patterns of the energetic and oxidative metabolism, extracellular matrix regulation and inflammation pathways, ageing seems to be gender and tissue-specific in general. Additionally, there is an age-related implication in several numbers of impaired events on the normal alternative splicing process. On the other hand, there is a direct relation of several non-coding RNA molecules with age-related changes which indicates its possible use as biomarkers for diagnostics and therapeutically purposes. Together, these findings highlight the importance to continue focusing research on RNA studies to improve our knowledge in the pathophysiology of age-related diseases.
Transformerless inverters have an important role in the electrical energy market. The high-efficiency and reliable inverter concept is one of the most widely used inverters in single-phase ...photovoltaic systems because of its high efficiency, low cost, and reduced leakage ground current. However, the leakage ground current behavior depends on the power and weather conditions, which can increase the parasitic capacitance value, thus producing an increase in the leakage ground current magnitude. In this paper, it is proposed to add a passive inductive–capacitive output filter to the inverter structure in order to reduce the dependency of the leakage ground current on the system power and weather conditions. The inductive–capacitive output filter is designed in such a way that it can provide a low impedance path for the leakage ground current, different from the ground path. The proposed system was evaluated both through simulations and experimentally in a 1 kW laboratory prototype.
PDF
