Aim: This study firstly evaluated the activity of a silver nanoparticle (AgNPs) solution against Candida albicans and then the effect of incorporation of AgNPs into a denture base acrylic resin on ...the material’s hydrophobicity, C. albicans adhesion and biofilm formation. Methods and Results: The AgNPs solution was synthesized by chemical reduction and characterized. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations for planktonic cells and sessile cells (MFCs) of the AgNPs solution against C. albicans were determined. Specimens (n = 360) of silver‐incorporated acrylic resin at concentrations of 1000, 750, 500, 250 and 30 ppm were also prepared and stored in PBS for 0, 7, 90 and 180 days. Control was acrylic resin without AgNPs (0 ppm). After the storage periods, contact angles were measured and the specimens were used for C. albicans adherence (37°C; 90 min; n = 9) and biofilm formation (37°C; 48 h; n = 9) by XTT reduction assay. MIC, MFC and MFCs values were 3·98, 15·63 and 1000 ppm, respectively. Incorporation of AgNPs reduced the hydrophobicity of the resin. No effect on adherence and biofilm formation was observed. At 90 and 180 days of storage, there was significant increase in adherence and biofilm formation. Conclusions: Although the AgNPs solution had antifungal activity, no effect on C. albicans adherence and biofilm formation was observed after its incorporation into a denture base resin. Significance and Impact of the Study: The synthesized AgNPs solution is a promising antifungal agent, warranting investigations of more efficient methods of incorporation into denture base resins.
In this randomized clinical trial, the clinical and mycological efficacy of Photodynamic Therapy (PDT) was compared with that of topical antifungal therapy for the treatment of denture stomatitis ...(DS) and the prevalence of Candida species was identified. Patients were randomly assigned to one of two groups (n = 20 each); in the nystatin (NYT) group patients received topical treatment with nystatin (100 000 IU) four times daily for 15 days and in the PDT group the denture and palate of patients were sprayed with 500 mg/L of Photogem®, and after 30 min of incubation, were illuminated by light emitting-diode light at 455 nm (37.5 and 122 J/cm2, respectively) three times a week for 15 days. Mycological cultures taken from dentures and palates and standard photographs of the palates were taken at baseline (day 0), at the end of the treatment (day 15) and at the follow-up time intervals (days 30, 60 and 90). Colonies were quantified (CFU/mL) and identified by biochemical tests. Data were analysed by Fisher's exact test, analysis of variance and Tukey tests and κ test (α = 0.05). Both treatments significantly reduced the CFU/mL at the end of the treatments and on day 30 of the follow-up period (p <0.05). The NYT and PDT groups showed clinical success rates of 53% and 45%, respectively. Candida albicans was the most prevalent species identified. PDT was as effective as topical nystatin in the treatment of DS.
This study evaluated the photodynamic inactivation (PDI) mediated by Photodithazine
®
(PDZ) against 15 clinical isolates of Candida albicans, Candida glabrata and Candida tropicalis. Each isolate, in ...planktonic and biofilm form, was exposed to PDI by assessing a range of PDZ concentrations and light emitting diode fluences. Cell survival of the planktonic suspensions was determined by colony forming units (CFU ml
−1
). The antifungal effects of PDI against biofilms were evaluated by CFU ml
−1
and metabolic assay. Data were analyzed by non-parametric tests (α = 0.05). Regardless of the species, PDI promoted a significant viability reduction of planktonic yeasts. The highest reduction in cell viability of the biofilms was equivalent to 0.9 log
10
(CFU ml
−1
) for C. albicans, while 1.4 and 1.5 log
10
reductions were obtained for C. tropicalis and C. glabrata, respectively. PDI reduced the metabolic activity of biofilms by 62.1, 76.0, and 76.9% for C. albicans, C. tropicalis, and C. glabrata, respectively. PDZ-mediated PDI promoted significant reduction in the viability of Candida isolates.
In this work the cold gas spray (CGS) technique was used to produce anti-biofilm and biocompatibility copper surfaces. Copper was deposited onto carbon steel and the phase composition, morphology, ...porosity, and adherence of the coating were investigated. Methicillin-susceptible Staphylococcus aureus (MSSA-ATCC 25923), Escherichia coli (ATCC 25922) and Candida albicans (SC 5314) biofilms were used in the antimicrobial tests, and the biocompatibility of the coatings was tested in human monocytes (THP-1 cell line). The main physical characteristics of the copper coating were thickness (640 ± 5) μm, porosity 0.4% and adherence 30 MPa. The X-ray diffraction pattern of the coatings showed only crystalline phase of copper. The plate spreading method, XTT assay, confocal fluorescence microscopy and scanning electron microscopy images showed that copper coatings were effective as an antimicrobial agent by blocking the adhesion and viability of C. albicans and killing the surface adhered bacterial strains, significantly reducing the biofilm survival. The viability rate of THP-1 cells for 24 h was 95% and the confocal microscopy showed the same labeling as the standard culture conditions.
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•Copper powders were sprayed on carbon steel using Cold Gas Spray (CGS).•Copper coatings are dense with low porosity and high adhesion.•CGS copper coatings showed antibacterial and fungicidal activity.•CGS copper coatings showed biocompatibility for THP-1 cells.
Abstract
The ability to manipulate the structure and function of promising systems via external stimuli is emerging with the development of reconfigurable and programmable multifunctional materials. ...Increasing antifungal and antitumor activity requires novel, effective treatments to be diligently sought. In this work, the synthesis, characterization, and
in vitro
biological screening of pure α-Ag
2
WO
4
, irradiated with electrons and with non-focused and focused femtosecond laser beams are reported. We demonstrate, for the first time, that Ag nanoparticles/α-Ag
2
WO
4
composite displays potent antifungal and antitumor activity. This composite had an extreme low inhibition concentration against
Candida albicans
, cause the modulation of α-Ag
2
WO
4
perform the fungicidal activity more efficient. For tumor activity, it was found that the composite showed a high selectivity against the cancer cells (MB49), thus depleting the populations of cancer cells by necrosis and apoptosis, without the healthy cells (BALB/3T3) being affected.
Summary The effectiveness of microwave disinfection of maxillary complete dentures on the treatment of Candida‐related denture stomatitis was evaluated. Patients (n = 60) were randomly assigned to ...one of four treatment groups of 15 subjects each; Control group: patients performed the routine denture care; Mw group: patients had their upper denture microwaved (650 W per 6 min) three times per week for 30 days; group MwMz: patients received the treatment of Mw group in conjunction with topical application of miconazole three times per day for 30 days; group Mz: patients received the antifungal therapy of group MwMz. Cytological smears and mycological cultures were taken from the dentures and the palates of all patients before treatment at day 15 and 30 of treatment and at follow‐up (days 60 and 90). The effectiveness of the treatments was evaluated by Kruskal–Wallis and Mann–Whitney tests. Microbial and clinical analysis of the control group demonstrated no significant decrease in the candidal infection over the clinical trial. Smears and cultures of palates and dentures of the groups Mw and MwMz exhibited absence of Candida at day 15 and 30 of treatment. On day 60 and 90, few mycelial forms were observed on 11 denture smears (36·6%) from groups Mw and MwMz, but not on the palatal smears. Miconazole (group Mz) neither caused significant reduction of palatal inflammation nor eradicated Candida from the dentures and palates. Microwaving dentures was effective for the treatment of denture stomatitis. The recurrence of Candida on microwaved dentures at follow‐up was dramatically reduced.
Summary
There is a propensity for fungal adherence to the polymethylmethacrylate used for making denture bases. Therefore, this study investigated whether surface modifications with plasma treatments ...would reduce the adherence of Candida albicans to a denture base resin. Samples (n = 180) with smooth and rough surfaces were made and divided into five groups: control – non‐treated; experimental groups – submitted to plasma treatments to obtain surfaces with different hydrophobicities (Ar/50 W; ArO2/70 W; AAt/130 W) or with incorporated fluoride (Ar/SF670 W). Contact angles were measured immediately after treatments and after samples were immersed in water for 48 h. For each group, half the samples were incubated with saliva before the adherence test. The number of adhered C. albicans was evaluated by counting after crystal violet staining. The plasma treatments were effective in modifying the polymethylmethacrylate surface. However, there was a significant alteration in the contact angle measured after immersion in water. No statistically significant difference in the adherence of C. albicans was observed between the experimental and control groups, irrespective of the presence or absence of saliva, and surface roughness.
Enamel specimens need to be disinfected before being used in intraoral appliances and the disinfection method must not affect the integrity of the tooth. This study evaluated the effectiveness of ...microwave bovine enamel disinfection and its effect on the surface microhardness (SMH) and cross-sectional microhardness (CSMH) of sound, demineralized and remineralized enamel. Forty enamel specimens were divided into two groups (n = 20): control (nonirradiated) and microwave. Microwave specimens were immersed in water and irradiated (650 W/3 min). Specimens were incubated at 37°C for 7 days under aerobic and anaerobic conditions. An aliquot was removed for counting, plated at serial dilutions on blood agar plates, which were incubated aerobically and anaerobically. The colony-forming units were calculated. Other 20 enamel specimens (4 × 4 mm) were embedded in acrylic resin, polished and sliced into two halves: one of the fragments was reserved as control while the other was microwaved. SMH and CSMH of sound enamel were analyzed for both groups. The CSMH was further evaluated after demineralization and remineralization cycles. The Knoop integral (ΔKI) was also calculated. Data were analyzed statistically by paired Student’s t test (α = 0.05). While control specimens showed microbial growth, those microwaved were disinfected. There were no significant differences in SMH, CSMH and ΔKI between the control and microwave groups. Microwave irradiation for 3 min at 650 W was effective for the complete disinfection of bovine enamel specimens without affecting the SMH and CSMH of sound, demineralized and remineralized enamel.
Context.
Gravitational wave (GW) astronomy has rapidly reached maturity, becoming a fundamental observing window for modern astrophysics. The coalescences of a few tens of black hole (BH) binaries ...have been detected, while the number of events possibly including a neutron star (NS) is still limited to a few. On 2019 August 14, the LIGO and Virgo interferometers detected a high-significance event labelled S190814bv. A preliminary analysis of the GW data suggests that the event was likely due to the merger of a compact binary system formed by a BH and a NS.
Aims.
In this paper, we present our extensive search campaign aimed at uncovering the potential optical and near infrared electromagnetic counterpart of S190814bv. We found no convincing electromagnetic counterpart in our data. We therefore use our non-detection to place limits on the properties of the putative outflows that could have been produced by the binary during and after the merger.
Methods.
Thanks to the three-detector observation of S190814bv, and given the characteristics of the signal, the LIGO and Virgo Collaborations delivered a relatively narrow localisation in low latency – a 50% (90%) credible area of 5 deg
2
(23 deg
2
) – despite the relatively large distance of 267 ± 52 Mpc. ElectromagNetic counterparts of GRAvitational wave sources at the VEry Large Telescope collaboration members carried out an intensive multi-epoch, multi-instrument observational campaign to identify the possible optical and near infrared counterpart of the event. In addition, the ATLAS, GOTO, GRAWITA-VST, Pan-STARRS, and VINROUGE projects also carried out a search on this event. In this paper, we describe the combined observational campaign of these groups.
Results.
Our observations allow us to place limits on the presence of any counterpart and discuss the implications for the kilonova (KN), which was possibly generated by this NS–BH merger, and for the strategy of future searches. The typical depth of our wide-field observations, which cover most of the projected sky localisation probability (up to 99.8%, depending on the night and filter considered), is
r
∼ 22 (resp.
K
∼ 21) in the optical (resp. near infrared). We reach deeper limits in a subset of our galaxy-targeted observations, which cover a total ∼50% of the galaxy-mass-weighted localisation probability. Altogether, our observations allow us to exclude a KN with large ejecta mass
M
≳ 0.1
M
⊙
to a high (> 90%) confidence, and we can exclude much smaller masses in a sub-sample of our observations. This disfavours the tidal disruption of the neutron star during the merger.
Conclusions.
Despite the sensitive instruments involved in the campaign, given the distance of S190814bv, we could not reach sufficiently deep limits to constrain a KN comparable in luminosity to AT 2017gfo on a large fraction of the localisation probability. This suggests that future (likely common) events at a few hundred megaparsecs will be detected only by large facilities with both a high sensitivity and large field of view. Galaxy-targeted observations can reach the needed depth over a relevant portion of the localisation probability with a smaller investment of resources, but the number of galaxies to be targeted in order to get a fairly complete coverage is large, even in the case of a localisation as good as that of this event.
Pure hydroxyapatite (HA) and hydroxyapatite decorated with silver (HA@Ag) nanoparticles were synthesized and characterized. The antifungal effect of HA@Ag nanoparticles in a distilled water solution ...was evaluated against Candida albicans. The origin of the antifungal activity of the HA@Ag is also discussed. The results obtained showed that the HA nanorod morphology remained the same with Ag ions decorations on the HA structure which were deposited in the form of nanospheres. Interaction where occurred between the structure and its defect density variation in the interfacial HA@Ag and intrafacial HA region with the fungal medium resulted in antifungal activity. The reaction mechanisms involved oxygen and water adsorption which formed an active complex cluster. The decomposition and desorption of the final products as well as the electron/hole recombination process have an important role in fungicidal effects.
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