Fenson residues were extracted from crop with acetone and, after partitioning into dichloromethane, the extract, was purified by Florisil column chromatography. The column was eluted with a ...hexane-dichloromethane-acetonitrile elution system, Fenson residues being eluted in the second fraction consisting of hexane-dichloromethane-acetonitrile (50 + 49,65 + 0,35). Identification and determination were carried out by GLC/ECD on a packed OV 17 + QF 1 column. Confirmation of residues was performed by TLC/GLC and by TLC-GC/ MS. Mean recovery of Fenson residues from apples was 87,8% with a variation coefficient of 5,8%.
ITALIANO: I residui di Fenson sono stati estratti con acetone e, dopo ripartizione in diclorometano, l'estratto e stato purificato mediante cromatografia su colonna di Florisil. La colonna e stata eluita con un sistema esano-diclorometano-acetonitrile. I residui di Fenson sono stati eluiti nella seconda frazione costituita da esano-diclorometano-acetonitrile (50 + 49,65 + 0,35). L'identificazione e determinazione sono state effettuate con gascromatografia a cattura di elettroni su una colonna impaccata di OV 17 + QF 1. La conferma del dato quali-quantitativo e stata realizzata mediante TLC/GLC e TLC-GC/MS. Il recupero medio da mele e stato 87,8%, con un cofficiente di variazione di 5,8%.
This paper describes the method which was developed in relation to analytical work connected with microbial and physico-chemical degradation experiments on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). ...Soil samples are best extracted with methanol plus methylene chloride. Microbial preparations are extracted with light petroleum after boiling with methanolic potassium hydroxide. The clean-up consists of a sulphuric acid treatment and chromatography on a multilayer column (Celite + H2SO4/silica gel) followed by alumina column chromatography. The clean-up procedure proved to be suitable for soil samples and microbial preparations even when large quantities of organic matter (hydrocarbons, oils, surfactants) were present.
Fenson residues were extracted from crop with acetone and, after partitioning into dichloromethane, the extract, was purified by Florisil column chromatography. The column was eluted with a ...hexane-dichloromethane-acetonitrile elution system, Fenson residues being eluted in the second fraction consisting of hexane-dichloromethane-acetonitrile (50 + 49,65 + 0,35). Identification and determination were carried out by GLC/ECD on a packed OV 17 + QF 1 column. Confirmation of residues was performed by TLC/GLC and by TLC-GC/ MS. Mean recovery of Fenson residues from apples was 87,8% with a variation coefficient of 5,8%.
I residui di Fenson sono stati estratti con acetone e, dopo ripartizione in diclorometano, l'estratto e stato purificato mediante cromatografia su colonna di Florisil. La colonna e stata eluita con un sistema esano-diclorometano-acetonitrile. I residui di Fenson sono stati eluiti nella seconda frazione costituita da esano-diclorometano-acetonitrile (50 + 49,65 + 0,35). L'identificazione e determinazione sono state effettuate con gascromatografia a cattura di elettroni su una colonna impaccata di OV 17 + QF 1. La conferma del dato quali-quantitativo e stata realizzata mediante TLC/GLC e TLC-GC/MS. Il recupero medio da mele e stato 87,8%, con un cofficiente di variazione di 5,8%.
