Gold nanoparticles (NPs) efficiently quench adsorbed fluorophores. Upon disruption of such complexes by an analyte, fluorescence turn‐on is observed. By judicious choice of the functionalized NP and ...the fluorophore, these complexes display different responses to analytes, thus leading to versatile yet simple array‐based sensor platforms. Using this strategy, we can identify proteins in buffer and serum, distinguish between both different species and different strains of bacteria, and differentiate between healthy, cancerous, and metastatic human and murine cells.
Disruption desired: Different monolayer‐protected nanoparticle–fluorophore constructs are used in indicator‐displacement assays to spy on proteins, bacteria, cells, and ions (see picture). The modus operandi involves disruption of the preformed quencher–fluorophore complexes, leading to partial and analyte‐dependent fluorescence turn‐on. Small libraries of nanoparticle–fluorophore complexes get the sensing job for different biological analytes done.
We address the astrophysical imaging of a family of deformed Kerr black holes (BHs). These are stationary, asymptotically flat BH spacetimes that are solutions of general relativity minimally coupled ...to a massive, complex scalar field: Kerr BHs with scalar hair (KBHsSH). Such BHs bifurcate from the vacuum Kerr solution and can be regarded as a horizon within a rotating boson star. In a recent letter P. V. P. Cunha, C. A. R. Herdeiro, E. Radu, and H. F. Rúnarsson, Phys. Rev. Lett. 115, 211102 (2015)., it was shown that KBHsSH can exhibit very distinct shadows from the ones of their vacuum counterparts. The setup therein, however, considers the light source to be a celestial sphere sufficiently far away from the BH. Here, we analyze KBHsSH surrounded by an emitting torus of matter simulating a more realistic astrophysical environment, and study the corresponding lensing of light as seen by a very faraway observer, to appropriately model ground-based observations of Sgr A*. We find that the differences in imaging between KBHsSH and comparable vacuum Kerr BHs remain, albeit less dramatic than those observed for the corresponding shadows in the previous setup. In particular, we highlight two observables that might allow differentiating KBHsSH and Kerr BHs. The first is the angular size of the photon ring (in a Kerr spacetime) or lensing ring (in a KBHSH spacetime), the latter being significantly smaller for sufficiently non-Kerr-like spacetimes. The second is the existence of an edge in the intensity distribution (the photon ring in Kerr spacetime). This edge can disappear for very non-Kerr-like KBHsSH. It is plausible, therefore, that sufficiently precise very long baseline interferometric observations of BH candidates can constrain this model.
Context.
The Event Horizon Telescope (EHT) collaboration recently obtained the first images of the surroundings of the supermassive compact object M87* at the center of the galaxy M87. This provides ...a fascinating probe of the properties of matter and radiation in strong gravitational fields. It is important to determine from the analysis of these results what can and cannot be inferred about the nature of spacetime around M87*
Aims.
We want to develop a simple analytic disk model for the accretion flow of M87*. Compared to general-relativistic magnetohydrodynamic models, this new approach has the advantage that it is independent of the turbulent character of the flow and is controlled by only a few easy-to-interpret, physically meaningful parameters. We want to use this model to predict the image of M87*, assuming that it is either a Kerr black hole or an alternative compact object.
Methods.
We computed the synchrotron emission from the disk model and propagate the resulting light rays to the far-away observer by means of relativistic ray tracing. Such computations were performed assuming different spacetimes, such as Kerr, Minkowski, nonrotating ultracompact star, rotating boson star, or Lamy spinning wormhole. We performed numerical fits of these models to the EHT data.
Results.
We discuss the highly lensed features of Kerr images and show that they are intrinsically linked to the accretion-flow properties and not only to gravitation. This fact is illustrated by the notion of the secondary ring, which we introduce. Our model of a spinning Kerr black hole predicts mass and orientation consistent with the EHT interpretation. The non-Kerr images result in a similar quality of numerical fits and may appear very similar to Kerr images, once blurred to the EHT resolution. This implies that a strong test of the Kerr spacetime may be out of reach with the current data. We note that future developments of the EHT could alter this situation.
Conclusions.
Our results show the importance of studying alternatives to the Kerr spacetime to be able to test the Kerr paradigm unambiguously. More sophisticated treatments of non-Kerr spacetimes and more advanced observations are needed to proceed further in this direction.
Rapid and sensitive detection of pathogens is a key requirement for both environmental and clinical settings. We report here a colorimetric enzyme–nanoparticle conjugate system for detection of ...microbial contamination. In this approach, cationic gold nanoparticles (NPs) featuring quaternary amine headgroups are electrostatically bound to an enzyme β-galactosidase (β-Gal), inhibiting enzyme activity. Analyte bacteria bind to the NP, which releases the β-Gal and restores its activity, providing an enzyme-amplified colorimetric readout of the binding event. Using this strategy, we have been able to quantify bacteria at concentrations of 1 × 102 bacteria/mL in solution and 1 × 104 bacteria/mL in a field-friendly test strip format.
A sensor array containing six non-covalent gold nanoparticle-fluorescent polymer conjugates has been created to detect, identify and quantify protein targets. The polymer fluorescence is quenched by ...gold nanoparticles; the presence of proteins disrupts the nanoparticle-polymer interaction, producing distinct fluorescence response patterns. These patterns are highly repeatable and are characteristic for individual proteins at nanomolar concentrations, and can be quantitatively differentiated by linear discriminant analysis (LDA). Based on a training matrix generated at protein concentrations of an identical ultraviolet absorbance at 280 nm (A280 = 0.005), LDA, combined with ultraviolet measurements, has been successfully used to identify 52 unknown protein samples (seven different proteins) with an accuracy of 94.2%. This work demonstrates the construction of novel nanomaterial-based protein detector arrays with potential applications in medical diagnostics.
Supramolecular complexes of a family of positively charged conjugated polymers (CPs) and green fluorescent protein (GFP) create a fluorescence resonance energy transfer (FRET)-based ratiometric ...biosensor array. Selective multivalent interactions of the CPs with mammalian cell surfaces caused differential change in FRET signals, providing a fingerprint signature for each cell type. The resulting fluorescence signatures allowed the identification of 16 different cell types and discrimination between healthy, cancerous, and metastatic cells, with the same genetic background. While the CP-GFP sensor array completely differentiated between the cell types, only partial classification was achieved for the CPs alone, validating the effectiveness of the ratiometric sensor. The utility of the biosensor was further demonstrated in the detection of blinded unknown samples, where 121 of 128 samples were correctly identified. Notably, this selectivity-based sensor stratified diverse cell types in minutes, using only 2000 cells, without requiring specific biomarkers or cell labeling.
Context. The first observations of the GRAVITY instrument obtained in 2016, have shown that it should become possible to probe the spacetime close to the supermassive black hole Sagittarius A* (Sgr ...A*) at the Galactic center by using accurate astrometric positions of the S2 star. Aims. The goal of this paper is to investigate the detection by GRAVITY of different relativistic effects affecting the astrometric and/or spectroscopic observations of S2 such as the transverse Doppler shift, the gravitational redshift, the pericenter advance and higher-order general relativistic (GR) effects, in particular the Lense-Thirring effect due to the angular momentum of the black hole. Methods. We implement seven stellar-orbit models to simulate both astrometric and spectroscopic observations of S2 beginning near its next pericenter passage in 2018. Each model takes into account a certain number of relativistic effects. The most accurate one is a fully GR model and is used to generate the mock observations of the star. For each of the six other models, we determine the minimal observation times above which it fails to fit the observations, showing the effects that should be detected. These threshold times are obtained for different astrometric accuracies as well as for different spectroscopic errors. Results. Transverse Doppler shift and gravitational redshift can be detected within a few months by using S2 observations obtained with pairs of accuracies (σA,σV) = (10−100 μas, 1−10 km s-1) where σA and σV are the astrometric and spectroscopic accuracies, respectively. Gravitational lensing can be detected within a few years with (σA,σV) = (10 μas, 10 km s-1). Pericenter advance should be detected within a few years with (σA,σV) = (10 μas, 1−10 km s-1). Cumulative high-order photon curvature contributions, including the Shapiro time delay, affecting spectroscopic measurements can be observed within a few months with (σA,σV) = (10 μas, 1 km s-1). By using a stellar-orbit model neglecting relativistic effects on the photon path except the major contribution of gravitational lensing, S2 observations obtained with accuracies (σA,σV) = (10 μas, 10 km s-1), and a black hole angular momentum (a,i′,Ω′) = (0.99,45°,160°), the 1σ error on the spin parameter a is of about 0.4, 0.2, and 0.1 for a total observing run of 16, 30, and 47 yr, respectively. The 1σ errors on the direction of the angular momentum reach σi′ ≈ 25° and σΩ′ ≈ 40° when considering the three orbital periods run. We found that the uncertainties obtained with a less spinning black hole (a = 0.7) are similar to those evaluated with a = 0.99. Conclusions. The combination of S2 observations obtained with the GRAVITY instrument and the spectrograph SINFONI (Spectrograph for INtegral Field Observations in the Near Infrared) also installed at the VLT (Very Large Telescope) will lead to the detection of various relativistic effects. Such detections will be possible with S2 monitorings obtained within a few months or years, depending on the effect. Strong constraints on the angular momentum of Sgr A* (e.g., at 1σ = 0.1) with the S2 star will be possible with a simple stellar-orbit model without using a ray-tracing code but with approximating the gravitational lensing effect. However, long monitorings are necessary, and we thus must rely on the discovery of closer-in stars near Sgr A* if we want to efficiently constrain the black hole parameters with stellar orbits in a short time, or monitor the flares if they orbit around the black hole.
Nephridiophagids are unicellular eukaryotes that parasitize the Malpighian tubules of numerous insects. Their life cycle comprises multinucleate vegetative plasmodia that divide into oligonucleate ...and uninucleate cells, and sporogonial plasmodia that form uninucleate spores. Nephridiophagids are poor in morphological characteristics, and although they have been tentatively identified as early-branching fungi based on the SSU rRNA gene sequences of three species, their exact position within the fungal tree of live remained unclear. In this study, we describe two new species of nephridiophagids (Nephridiophaga postici and Nephridiophaga javanicae) from cockroaches. Using long-read sequencing of the nearly complete rDNA operon of numerous further species obtained from cockroaches and earwigs to improve the resolution of the phylogenetic analysis, we found a robust affiliation of nephridiophagids with the Chytridiomycota-a group of zoosporic fungi that comprises parasites of diverse host taxa, such as microphytes, plants, and amphibians. The presence of the same nephridiophagid species in two only distantly related cockroaches indicates that their host specificity is not as strict as generally assumed.
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