Biosensing relies on the detection of molecules and their specific interactions. It is therefore highly desirable to develop transducers exhibiting ultimate detection limits. Microcavities are an ...exemplary candidate technology for demonstrating such a capability in the optical domain and in a label-free fashion. Additional sensitivity gains, achievable by exploiting plasmon resonances, promise biosensing down to the single-molecule level. Here, we introduce a biosensing platform using optical microcavity-based sensors that exhibits single-molecule sensitivity and is selective to specific single binding events. Whispering gallery modes in glass microspheres are used to leverage plasmonic enhancements in gold nanorods for the specific detection of nucleic acid hybridization, down to single 8-mer oligonucleotides. Detection of single intercalating small molecules confirms the observation of single-molecule hybridization. Matched and mismatched strands are discriminated by their interaction kinetics. Our platform allows us to monitor specific molecular interactions transiently, hence mitigating the need for high binding affinity and avoiding permanent binding of target molecules to the receptors. Sensor lifetime is therefore increased, allowing interaction kinetics to be statistically analysed.
Optical resonator biosensors are emerging as one of the most sensitive microsystem biodetection technology that does not require amplification or labeling of the analyte. This minireview provides a ...scholarly introduction to this research area and reviews current advances in molecular diagnostics and nanoparticle detection.
Probing individual chemical reactions is key to mapping reaction pathways. Trace analysis of sub-kDa reactants and products is obfuscated by labels, however, as reaction kinetics are inevitably ...perturbed. The thiol-disulfide exchange reaction is of specific interest as it has many applications in nanotechnology and in nature. Redox cycling of single thiols and disulfides has been unresolvable due to a number of technological limitations, such as an inability to discriminate the leaving group. Here, we demonstrate detection of single-molecule thiol-disulfide exchange using a label-free optoplasmonic sensor. We quantify repeated reactions between sub-kDa thiolated species in real time and at concentrations down to 100's of attomolar. A unique sensing modality is featured in our measurements, enabling the observation of single disulfide reaction kinetics and pathways on a plasmonic nanoparticle surface. Our technique paves the way towards characterising molecules in terms of their charge, oxidation state, and chirality via optoplasmonics.
Abstract
Microlasers, relying on the strong coupling between active particles and optical microcavity, exhibit fundamental differences from conventional lasers, such as multi-threshold/thresholdless ...behavior and nonclassical photon emission. As light sources, microlasers possess extensive applications in precision measurement, quantum information processing, and biochemical sensing. Here we propose a whispering-gallery-mode microlaser scheme, where ultracold alkaline-earth metal atoms, i.e., gain medium, are tightly confined in a two-color evanescent lattice that is in the ring shape and formed around a microsphere. To suppress the influence of the lattice-induced ac Stark shift on the moderately-narrow-linewidth laser transition, the red-detuned trapping beams operate at a magic wavelength while the wavelength of the blue-detuned trapping beam is set close to the other magic wavelength. The tiny mode volume and high quality factor of the microsphere ensure the strong atom-microcavity coupling in the bad-cavity regime. As a result, both saturation photon and critical atom numbers, which characterize the laser performance, are substantially reduced below unity. We explore the lasing action of the coupled system by using the Monte Carlo approach. Our scheme may be potentially generalized to the microlasers based on the forbidden clock transitions, holding the prospect for microscale active optical clocks in precision measurement and frequency metrology.
Researchers in the field of whispering-gallery-mode (WGM) microresonators have proposed biointegrated low-threshold WGM lasers, to enable large-scale parallel single-cell tracking and barcoding. ...Although the reported devices have so far been primarily investigated in model applications, most recent results represent important steps towards the development of in vivo tags and sensors that utilize the unique and narrow spectral features of miniature WGM lasers.
Lasers are the pillars of modern optics and sensing. Microlasers based on whispering-gallery modes (WGMs) are miniature in size and have excellent lasing characteristics suitable for biosensing. WGM ...lasers have been used for label-free detection of single virus particles, detection of molecular electrostatic changes at biointerfaces, and barcode-type live-cell tagging and tracking. The most recent advances in biosensing with WGM microlasers are described in this review. We cover the basic concepts of WGM resonators, the integration of gain media into various active WGM sensors and devices, and the cutting-edge advances in photonic devices for micro- and nanoprobing of biological samples that can be integrated with WGM lasers.
Active optomechanics Yu, Deshui; Vollmer, Frank
Communications physics,
03/2022, Letnik:
5, Številka:
1
Journal Article
Recenzirano
Odprti dostop
Abstract
Cavity optomechanics explores the coupling between optical and mechanical modes mediated by the radiation pressure force. Unlike the passive scheme, the active optomechanics with optical ...gain directly imposes the mechanical motion upon the lasing dynamics, unveiling the intrinsic properties determined by the system itself. Here we numerically explore the general characteristics of the active optomechanics. The effects of the mechanical oscillation on the macroscopic laser include introducing multiple unstable regimes in the lasing phase, shifting the laser central frequency, broadening the laser spectrum, and degrading the laser frequency stability. Reducing the optical gain down to one active atom highlights the quantum nature of atom–cavity and photon–phonon interactions. The one-atom optomechanical microlaser does not only emit nonclassical photons but also generate nonclassical photon–phonon pairs. Our work extends the cavity optomechanics to the active fashion, paving the way towards optomechanical light sources for photonic integrated circuits, on-chip quantum communication, and biosensing.
A new label‐free sensing mechanism is demonstrated experimentally by monitoring the whispering‐gallery mode broadening in microcavities. It is immune to both noise from the probe laser and ...environmental disturbances, and is able to remove the strict requirement for ultra‐high‐Q mode cavities for sensitive nanoparticle detection. This ability to sense nanoscale objects and biological analytes is particularly crucial for wide applications.
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