Cationic supramolecules composed of multiple oligoethylenimine‐grafted β‐cyclodextrins that are threaded and blocked on a triblock copolymer chain (see figure) are synthesized as ...gene‐delivery vectors. The supramolecules contain many cationic cyclic units threaded on a polymer chain to form an integrated entity, functioning as a macromolecular gene vector with good DNA binding ability, low cytotoxicity, and high gene‐transfection efficiency.
Ideal metallic biomaterials for use in implants are required to meet the mechanical compatibility, i.e., low Young's modulus combined with high mechanical strength, however, these constraints are ...generally contradictory. Here, an exceptional combination of ultralow Youngʹs modulus of E~45GPa, ultrahigh tensile strength of σb~1185MPa and ultrahigh elastic admissible strain of δ~2.2% is achieved in a new high Zr-containing Ti-based alloy (Ti-50Zr-5Al-4V (wt%)), which is subjected to solution treatment and cold rolling. The ultralow Youngʹs modulus is attributed to a mixture of bcc β and orthorhombic α″ phases while the ultrahigh strength results from the solution strengthening and work hardening. This study can be of significance for developing excellent removable implant materials.
Nonaqueous capillary electrophoresis (NACE) is very well suited for online coupling with mass spectrometry due to the relatively high volatility and low surface tension of most organic solvents. Here ...we present a quantitative NACE-ESI-MS/MS method for separating and determining physcion, chrysophanol, and aloe-emodin in rhubarb. Dantron was used as an internal standard to ensure accuracy and reproducibility in quantitative analyses. Parameters including the pH, background electrolyte (BGE) composition, flow-through microvial chemical modifier solution composition, and modifier solution flow rate were carefully optimized. The developed method was validated by assessing its precision, LODs, and linear range. The contents of physcion, chrysophanol, and aloe-emodin in rhubarb were determined to be 0.22%, 1.0%, and 0.17%, respectively.
This study aims to investigate the using of bone marrow mesenchymal stem cells (BMSCs) genetically engineered to produce interferon-β (IFN-β) as a gene delivery system to treat hepatocellular ...carcinoma (HCC) in vitro and in vivo.
To measure the effects on tumour cell growth in vitro, IFN-β-producing BMSCs (BMSC/IFN-β) were co-cultured with the HCC cell line HepG2 and Huh7. Enzyme-linked immunosorbent assay (ELISA) was used to detect the IFN-β secretion in the BMSC culture condition medium (CM). The effect of BMSC/IFN-β on HCC cells proliferation was examined both in vitro and in vivo by using MTT, colony formation assay, BrdU staining, cell cycle analysis, and xenografted NOD/SCID mouse tumour model. To examine the impact of BMSC/IFN-β on the AKT/FOXO3a signalling, RT-PCR and western blotting were performed.
The BMSC/IFN-β cells can stably secrete high levels of IFN-β. Both MTT and colony forming assay showed that HCC cells had a lower growth rate when cultured in BMSC/IFN-β-CM as compared with that in BMSC/vector-CM or DMEM culture group. Co-culture with BMSC/IFN-β-CM dramatically decreased the percentages of cells with incorporated BrdUrd. In BMSC/IFN-β-CM-treated HCC cells, the proportion of G1-phase cells increased but it decreased in the S phase of the cell. The BMSC/IFN-β inhibited HCC growth in NOD/SCID mice and proved the survival period of these mice. Compared with the control group, p21 and p27 expression of hepatoma cells increased, whereas cyclin D1 and phosphorylation of Rb expression decreased when co-cultured with BMSC/IFN-β-CM. It was associated with suppression of Akt activity and enhanced transcriptional activity of FOXO3a.
The IFN-β gene-modified BMSCs can effectively inhibit the proliferation of HCC cells in vitro and in vivo through inhibiting AKT/FOXO3a pathway. These results indicate that BMSC/IFN-β are a powerful anticancer cytotherapeutic tool for HCC.
Intracavity anomalous dispersion is investigated and proposed to enhance detection sensitivity of weak microwave electric field with Rydberg atoms. When the Rydberg atoms are prepared with Raman gain ...and the cavity field serves as the probe field driving the metastable-state transition, two cavity modes are amplified due to the gain effect and two gain peaks appear in the cavity transmission, meanwhile, a large anomalous dispersion arises in the gain window. The frequency splitting of two gain peaks shows linear relationship with the MW field strength, which can be used to probe the MW electric-field strength. It is interesting to find that the anomalous dispersion broadens the frequency splitting of two gain peaks dramatically, so the probe sensitivity of MW field could be enhanced by 10 times from simulation, compared with the cavity-less scheme.
The purpose of this study was to analyze the clinical characteristics of auditory neuropathy (AN) patients with normal hearing or mild hearing loss.
Data from Multicenter Study on Clinical Diagnosis ...and Intervention of Acoustic Neuropathy (registration number: ChiCTR2100050125). According to the Chinese clinical practice guideline of auditory neuropathy (version 2022), these patients divided into two groups: the normal hearing group (PTA Normal, PTA
group, the average hearing threshold<20 dB HL) and the mild hearing loss group (PTA Mild hearing loss, PTA
group, the average hearing threshold between 20-35 dBHL). The audiology characteristics, clinical features, and follow-up were analyzed. Data analysis was conducted using GraphPad Prism 8 and SPSS 20.0 software.
A total of 75 AN with normal hearing or mild hearing loss were included in this study. The PTA
group consisted of 19 patients (38 ears), including 12 males and 7 females. The average onset age was (16.9±4.5) years old, while the test age was (22.1±5.8
Purpose
The purpose of this study was to develop and validate a clinical predictive model for predicting the likelihood of a poor therapeutic response during the first year of recombinant human ...growth hormone (rhGH) treatment in children with growth disorders.
Methods
A total of 627 pediatric patients with growth disorders (GHD, ISS, TS, SGA) from The LG Growth Study cohort were evaluated. Restricted cubic splines (RCS) were utilized to investigate the association between predictors and the risk of poor rhGH response. Variables were selected using LASSO regression, and multivariate logistics regression models were established. Receiver operating characteristic (ROC) curves, calibration curves, decision curve analysis (DCA), and clinical impact curves (CIC) were used to assess the predictive model’s accuracy and clinical value. The predictive accuracy of the model was validated on the testing set.
Results
Two predictive models containing 8 baseline predictors (diagnosis, age, height SDS, bone age minus chronological age, rhGH dosage, distance from mid-parental height in SDS, weight SDS, IGF-1 SDS) and 1 post-treatment predictor (height SDS gain at 6 months) were constructed by multivariate logistic regression analyses. The nomogram was built based on the multivariate predictive model and showed good discrimination and model fit effects in both the training set and the testing set. DCA and CIC analyses presented good clinical usability.
Conclusion
The clinical predictive model for predicting the probability of poor short-term response of rhGH treatment in pediatric patients with growth disorders is useful and can assist physicians in making clinical decisions.
The interaction effect between the surface states of a topological insulator (TI) and a STM-coupled Anderson impurity is studied by using equations of motion of the Green's functions. Remarkably, we ...show that when a coupling between the Anderson impurity and the STM tip is included, the tunneling resonance and the Kondo peak can be tuned to be exactly at the Dirac point, by adjusting the impurity level and Fermi energy, such that the local density of states at the Dirac point is significantly enhanced. This is in contrast to the case of a STM-decoupled Anderson impurity, where both resonances are always fully suppressed at the Dirac point. Our finding suggests a pathway to experimentally control the fundamental properties of the electrons on a TI surface.
This letter reports an experimental evidence of stress induced martensitic phase transformation (SIPT) in Ti60Cu14Ni12Sn4Ta10 nanostructured composite by using in situ high-energy X-ray diffraction ...technology. It has been found that SIPT from NiTi B2 to NiTi B19' phase took place in the eutectic structure which was composed of nanoscale rod-like β-Ti and ordered B2 NiTi phase together with a small amount of B19 and B19' NiTi phases. The SIPT was accomplished in the elastic deformation period, suggesting that the enhanced elastic strain up to 1.92% is attributed to the SIPT together with the elastic deformation of both β-Ti and CuTi2 phases.
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•An in-situ synchrotron-based HE-XRD was used to study Ti60Cu14Ni12Sn4Ta10 alloy.•The stress induced phase transformation can be accomplished in the elastic stage.•The large elastic strain up to 1.92% is observed in the compressive deformation.
The crystalline microstructures, mechanical properties and structural stability of Ti-8.5Al-1.5Mo-xSi (wt%, x=0%, 0.2%, 0.4%, and 0.6%) alloys were comprehensively investigated. It is shown that with ...the addition of certain amount of Si, Widmanstatten microstructure was refined and high density of dislocation lines was accumulated in β film and at the grain boundaries between α and β phase. The addition of 0.2% and 0.4% Si induced suppression of the transformations of α→α2, α2→α and α→β in heating process and slow-down of the coarsening tendency of Widmanstatten microstructure during the annealing process. It has been found that the addition of Si results in enhanced tensile mechanical properties and overcomes the strength-ductility trade-off, e. g. the fracture strength and elongation of the alloy with 0.2% Si were increased by 10.5% and 68.5%, respectively, as compared with those of Si free alloy. The strengthening and toughening mechanism are attributed to the solid solution strengthening of Si in α and β phase, the grain boundary and Cottrel atmosphere strengthening, and remaining β phase induced toughening.
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