Ein hoch selektiver Katalysator für die Produktion von C5‐ bis C11‐Isoparaffinen aus Synthesegas beruht auf einem mesoporösen Zeolith. Die Selektivität von Ru/meso‐ZSM‐5 für diese Kohlenwasserstoffe ...reicht an 80 % heran, wobei das Verhältnis von Isoparaffinen zu n‐Paraffinen 2.7:1 beträgt. Die mesoporöse Struktur und die Acidität von meso‐ZSM‐5 sind entscheidend für die Produktselektivität in sekundären Hydrocrack‐Reaktionen.
The enantiomer of affinisine has been synthesized (from
l-tryptophan) with 100% diastereoselectivity via the asymmetric Pictet–Spengler reaction coupled with a Pd
0 (enolate mediated) coupling ...process. This enantiomer has also been converted into a key intermediate which provides a route to the enantiomers of both macroline and alstonerine following the previous work of LeQuesne.
A method for improving the performance of deep ultraviolet laser devices by improving the electron blocking layer is proposed. It is applied to deep ultraviolet semiconductor laser diodes through the ...left tapered electron blocking layer (EBL). Comparison with the right tapered electron blocking layer or the non-tapered electron blocking layer, the laser of left tapered electron blocking layer device exhibits higher efficiency at the time of device, indicating a significant increase in electron transfer and holes, which improves the luminous efficiency of the device.
To evaluate the effects of the fusion gene of ubiquitin (Ub) and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) M gene on the immune response in inoculated mice.
Mouse Ub gene and PRRSV ...M gene were amplified by RT-PCR from BALB/c mice spleen cells and PRRSV Ch-1a strain, respectively, and the M and Ub gene (U-M) was fused by SOE PCR. Therefore, pVAX1-U-M and pVAX1-M recombinant plasmid were constructed for eukaryotic expression.
The fusion U-M and M protein expressions were verified in transfected BHK-21 cells by indirect fluorescence assay. Furthermore, both pVAX1-M and pVAX1-U-M induced specific humoral and cellar immune responses against PRRSV in the recombinant plasmid injected mice. However, pVAX1-U-M was able to induce higher level of T cell response then that of pVAX1-M (P<0.05), but lower level of antibody (P<0.05).
Expression of U-M fusion gene had ability to enhance specific T cell response against PRRSV, but no effect on stimulation of humoral response in inoculated mice.
Equine interferon-gamma (eIFN-gamma) expressed both in E. coli and baculovirus were evaluated for antiviral activity against recombinant Vesicular Stomatits Virus expressing green fluorescence ...protein (rVSV-GFP) in EFK-78 cells. The assays were conducted in 96-well plate. Virus infectivity was measured by quantifying GFP-positive cells, instead of quantifying the CPE reduction. Prior to infection of EFK-78 cells with rVSV-GFP, the cells were incubated with eIFN-gamma. The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with eIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titers of antiviral activity were 1 x 10(3) AU/mL and 1 x 10(5) AU/mL of eIFN-gamma expressed from E. coli and baculovirus, respectively. The antiviral activities of the recombinant eIFN-gamma were highly efficient and specific, as it was blocked by mAbs against eIFN-gamma.
Prime-boost strategy has been exhibited its potency to enhance immune responses, which would be important to the success to develop a vaccine against the foot-and-mouth disease virus (FMDV). An ...eukaryotic expression construct encoding the FMDV capsid VP1 protein with a recombinant VP1 protein or a commercial FMDV vaccine were tested in the prime-boost strategy in mice and cattle trials. The levels of induced specific antibodies, T cell proliferations, and DTH activities were significantly higher in the prime-boost groups than in those vaccinated with DNA, protein or FMDV vaccine alone. More importantly, the levels of neutralizing antibodies in the former groups were significantly higher than others and could last for at least four months in cattle trials. This study suggests that the prime-boost strategy significantly improves the effective immunity and may provide a longer protection against FMDV infection.
To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.
On the basis of the ...obtained core sequence of open reading frame 1b of the coronavirus polymerase gene sequences, which was published in GenBank, using modified molecular beacon probe, artifical virus techinique and two different fragments amplification with different fluoresce, one set of primers and probe were designed. Then fluorescent PCR assays for specific and sensitive detection of the SARS virus was established, while the ELISA & the traditional method were used as control. 368 clinical specimens such as the throat swab, serum, feces, and urine from different cases, 52 cell cultures and 50 animal specimens were detected by the molecular beacon-based PCR.
The sensitivity of real -time PCR was 10 - 100 copies/ml, there was no cross reaction to other respiratory viruses such as influenza virus etc. Of 368 specimens, 20 were positive
Mobile agents in distributed virtual environments Ji Qingge; Hong Bingrong; Wang Dongmu
Proceedings Fourth International Conference/Exhibition on High Performance Computing in the Asia-Pacific Region,
2000, Letnik:
1
Conference Proceeding
Mobile agents provide a new method for distributed computing. This paper presents the advantages of using mobile agents in a DVE system, and describes the architecture of a distributed virtual ...environment system (DVE) designed to populate some mobile agents as well as stationary agents.
