Developing novel strategies against human colorectal cancer (CRC) cells is needed. Activation of AMP-activated protein kinase (AMPK) could possibly inhibit CRC cells. Protein kinase C ζ (PKCζ) is an ...AMPK negative regulator. Here we found that PKCζ expression was significantly elevated in human colon cancer tissues and CRC cells. PKCζ upregulation was correlated with AMPK in-activation and mTOR complex 1 (mTORC1) over-activation. Reversely, PKCζ shRNA knockdown activated AMPK signaling and inhibited HT-29 cell proliferation. Significantly, downregulation of microRNA-25-5p (miR-25-5p), a PKCζ-targeting miRNA, could be the cause of PKCζ upregulation. Exogenous expression of miR-25-5p silenced PKCζ to activate AMPK signaling, which inhibited HT-29 cell proliferation.
studies showed that HT-29 xenograft growth in mice was inhibited after expressing PKCζ shRNA or miR-25-5p. Collectively, PKCζ could be a novel oncogenic protein of human CRC. PKCζ silence, by targeted-shRNA or miR-25-5p expression, activates AMPK and inhibits HT-29 cell proliferation.
Postoperative gastrointestinal disorder (POGD) was a common complication after surgery under anesthesia. Strategies in combination with Traditional Chinese Medicine and Western medicine showed some ...distinct effects but standardized clinical practice guidelines were not available. Thus, a multidisciplinary expert team from various professional bodies including the Perioperative and Anesthesia Professional Committees of the Chinese Association of Integrative Medicine (CAIM), jointly with Gansu Province Clinical Research Center of Integrative Anesthesiology/Anesthesia and Pain Medical Center of Gansu Provincial Hospital of Traditional Chinese Medicine and WHO Collaborating Center for Guideline Implementation and Knowledge Translation/Chinese Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) Center/Gansu Provincial Center for Medical Guideline Industry Technology/Evidence‐based Medicine Center of Lanzhou University, was established to develop evidence‐based guidelines. Clinical questions (7 background and 12 clinical questions) were identified through literature reviews and expert consensus meetings. Based on systematic reviews/meta‐analyses, evidence quality was analyzed and the advantages and disadvantages of interventional measures were weighed with input from patients’ preferences. Finally, 20 recommendations were developed through the Delphi‐based consensus meetings. These recommendations included disease definitions, etiologies, pathogenesis, syndrome differentiation, diagnosis, and perioperative prevention and treatment.
3D braided shape memory composite (SMPC) tubes have great potential in designing smart structural components because of high inertia moment and near-net shape manufacturing. Here we developed a 3D ...braided carbon fiber reinforced shape memory polyurethane-based composite tube and investigated their thermal/electrical shape memory behaviors subjected to compression. The radial and axial compressive behaviors, thermally-electrically shape memory behaviors, and shape recovery forces of the SMPC tubes with different braiding angles were analyzed. The out-of-plane displacement and temperature field were obtained with 3D digital image correlation (DIC) and infrared thermography to characterize transverse compression deformation. We found that the 60° sample had the highest recovery force compared to the other braided angle samples, while the shape recovery speed was lower than small-angle samples. In the transverse compressive electro-thermal recovery test, the 30° sample reaches to glass transition temperature faster at the same voltage, and the 45° sample exhibits maximum shape recovery speed. The 3D braided SMPC tubes exhibited excellent electro-thermal shape memory behaviors and high recovery force, which were expected to extend the application of smart actuators.
Display omitted
•3D braided SMPC tubes with thermal-electrical stimuli recovery was prepared.•Influence of braiding angle on thermal/electrical shape recovery was presented.•Out-of-plane displacement and temperature fields in shape recovery were measured.
Electrical responses of three-dimensional braided composites under mechanical loads are important for the design of structural health monitoring in real time. Here we reported electrical properties ...and electrical-mechanical behaviors of three-dimensional braided composites with different braiding angles under three-point bending load, and investigated the correlation between electrical resistance and damage of the three-dimensional braided composites. We found that change of resistance reflected damage modes of the composite. In addition, we proposed an electrical-mechanical model to explain the resistance changes of three-dimensional braided composites based on the Weibull distribution function. The results of the model verify the effectiveness and rationality of the experimental results under bending load, which provides a theoretical basis for the structural health monitoring of three-dimensional braided composites through a electrical resistance change method.
Display omitted
The pencil-like ZnO hollow tubes with 9–12μm in length, 350–700nm in width, 200nm in wall thickness coating with g-C3N4 have been prepared via a chemical deposition process. As ...compared with uncoated ZnO or g-C3N4, these g-C3N4/ZnO composites showed the enhanced photocatalytic activity which can be attributed to the heterojunction structure. Furthermore, it is worth pointing out that the weight ratios of g-C3N4 to ZnO (g-C3N4/ZnO) played a significantly influence on the photodegradable properties. With increasing the mass ratio, the photocatalytic activity increased firstly and then decreased after reaching to an optimal photocatalytic performance. It can be inferred that the appreciation of g-C3N4 on the ZnO surface can improve the contact area which resulted in high separation of electrons and holes. However, excessive g-C3N4 may hinder the electrons transferring from the g-C3N4 to ZnO, and thus worse its photocatalytic performance. In our study, the g-C3N4/ZnO sample prepared with 10wt% of g-C3N4 exhibited the optimal photodegradable efficiency which 94% of Rhodamine B (RhB) has been degraded just in 2h.
To establish and validate a risk prediction model for perioperative ischemic complication (PIC) of endovascular treatment for ruptured anterior communicating artery aneurysms (ACoAAs).
The general ...clinical and morphologic data, operation schemes, and treatment outcomes of patients with ruptured ACoAAs treated with endovascular treatment in our center from January 2010 to January 2021 were retrospectively analyzed and assigned to primary (359 patients) and validation (67 patients) cohorts. A risk-predicted nomogram for PIC was developed through multivariate logistic regression analysis in the primary cohort. The discrimination ability, calibration accuracy, and clinical usefulness of the established PIC prediction model were evaluated and verified based on the receiver operating characteristic curves, calibration curves, and decision curve analysis in the primary and external validation cohorts, respectively.
A total of 426 patients were included, 47 of whom had PIC. The multivariate logistic regression analysis showed that hypertension, Fisher grade, A1 conformation, use of stent-assisted coiling, and aneurysm orientation were independent risk factors for PIC. Then, we developed a simple and easy-to-use nomogram to predict PIC. This nomogram has a good diagnostic performance (area under the curve, 0.773; 95% confidence interval, 0.685–0.862) and calibration accuracy; we then further validated this nomogram by external validation cohort and showed an excellent diagnostic performance and calibration accuracy. Besides, the decision curve analysis confirmed the clinical usefulness of the nomogram.
A history of hypertension, high preoperative Fisher grade, complete A1 conformation, use of stent-assisted coiling, and aneurysm orientation (pointing upward) are risk factors for PIC for ruptured ACoAAs. This novel nomogram might serve as a potential early warning sign of PIC for ruptured ACoAAs.
Inspired by the masked language modeling (MLM) in natural language processing tasks, the masked image modeling (MIM) has been recognized as a strong self-supervised pre-training method in computer ...vision. However, the high random mask ratio of MIM results in two serious problems: 1) the inadequate data utilization of images within each iteration brings prolonged pre-training, and 2) the high inconsistency of predictions results in unreliable generations, i.e., the prediction of the identical patch may be inconsistent in different mask rounds, leading to divergent semantics in the ultimately generated outcomes. To tackle these problems, we propose the efficient masked autoencoders with self-consistency (EMAE) to improve the pre-training efficiency and increase the consistency of MIM. In particular, we present a parallel mask strategy that divides the image into K non-overlapping parts, each of which is generated by a random mask with the same mask ratio. Then the MIM task is conducted parallelly on all parts in an iteration and the model minimizes the loss between the predictions and the masked patches. Besides, we design the self-consistency learning to further maintain the consistency of predictions of overlapping masked patches among parts. Overall, our method is able to exploit the data more efficiently and obtains reliable representations. Experiments on ImageNet show that EMAE achieves the best performance on ViT-Large with only 13% of MAE pre-training time using NVIDIA A100 GPUs. After pre-training on diverse datasets, EMAE consistently obtains state-of-the-art transfer ability on a variety of downstream tasks, such as image classification, object detection, and semantic segmentation.
•Dioscin showed anti-cancer effects on A549, NCI-H446 and NCI-H460 cancer cells.•Dioscin significantly induced cell apoptosis.•Dioscin caused DNA damage of the cancer cells.•Dioscin activated ...mitochondrial signal pathway of the cancer cells.
Dioscin, a natural steroid saponin, has been widely investigated. However, its anti-cancer activities on human lung cancer cells are still unknown. In the present paper, the inhibitory effects of dioscin were investigated, and the results showed that dioscin inhibited the proliferation of human A549, NCI-H446 and NCI-H460 cancer cells. DNA damage and cell apoptosis in dioscin-treated cells were found through single cell gel electrophoresis and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling assays. Furthermore, dioscin caused mitochondrial structure changes and blocked cell cycle at S phase based on transmission electron microscope and flow cytometry analysis. In addition, dioscin treatment caused the release of cytochrome c from mitochondria into cytosol. The activities of Caspase-3 and -9 in dioscin-treated groups were significantly increased compared with control group. Western blotting analysis showed that dioscin significantly down-regulated the expressions of Bcl-2 and Bcl-xl, and up-regulated the expressions of Bax, Bak and Bid. Our results indicate that dioscin has anticancer activities against human lung cancer cells through inducing cell cycle arrest, DNA damage and activating mitochondrial signal pathway.
...845 subjects were enrolled and randomized into the AGT (escitalopram or mirtazapine treatment) or TAU (treatment as usual) group, as described in previous publication. ...325 patients (GS = 177, ...NGS = 148) with completed baseline information were included, of whom 243 participants completed the 2-week follow-up (GS = 136, NGS = 107), 208 completed the 4-week follow-up (GS = 115, NGS = 93), 176 completed the 6-week follow-up (GS = 99, NGS = 77), 136 completed the 8-week follow-up (GS = 73, NGS = 63), and 125 completed the 12-week follow-up (GS = 67, NGS = 58). ...guilt, loss of weight, intelligence, cardiovascular symptoms, and respiratory symptoms on the HAM-D and HAM-A scales, and the mental condition and family relation item on the QoL scale were associated with genital symptoms in MDD patients. ...the GS group exhibited higher HAM-D and DSSS scores after two weeks of treatment, as well as at the 4-week follow-up, indicating that those with genital symptoms would experience a slower recovery from depressive and somatic symptoms in the acute phase of treatment.
As we have known that it is impossible to attenuate the vibration displacement of the floating-slab depending on a strong stiffness and to improve the low-frequency vibration isolation performance of ...the floating-slab track (FST) depending on a soft stiffness simultaneously in engineering. In this paper, we propose a novel method to solve the paradox by introducing a “Hardening-First-Then-Softening” (HFTS) design based upon the energy distribution of wheel-rail loads to formulate a model of nonlinear floating-slab isolator. The average procedure is employed to analyze the vibration isolation ability of the isolator with parameters taken from a metro line load excitation. A simulation model of train-FST-tunnel with HFTS isolator is constructed to study the dynamic behaviors of the system. The numerical simulations show the significant suppression of displacement response of floating-slab and Z weighted acceleration vibration level of the tunnel. The results presented herein this paper provide a scheme to solve the strict requirements of displacement suppression and the vibration isolation which is of beneficial to steady and silent urban rail transit.
PDF
