Most temperate wild bees overwinter in the larval or imaginal stage inside their nests after the entire larval food provisions have been consumed. Here, we report on the finding that
Colletes hederae
...(Colletidae), a late-flying European bee species active in September and October, passes the winter as half-grown larva inside the brood cell that still contains considerable amounts of stored food. We hypothesize that the special waterproof cell lining of
Colletes
reduces the risk of fungal infection, liquefaction, or desiccation of the stored food and thus liberated the bees from the need to consume the entire provisions before winter, allowing for the exploitation of late-flowering plant taxa.
Knowledge of patterns of genetic diversity in populations of threatened species is vital for their effective conservation. However, destructive sampling should be avoided in threatened species so as ...not to additionally increase the risk of local population extinction. We exclusively used beetle remains and beetles collected after death to analyze local and regional patterns of genetic variation in the endangered flightless longhorn beetle Iberodorcadion fuliginator in the border region of Switzerland, France and Germany, in grassland remnants. We extracted DNA from the beetles’ remains and genotyped 243 individuals at 6 microsatellite loci. We found moderate genetic differentiation between populations, each belonging to one of two metapopulations situated on either side of the river Rhine, but distinct genetic differentiation between populations across metapopulation. The genetic distance between populations was correlated with the geographic distance between the sites sampled. Genetic structure analysis inferred the presence of two genetic clusters. The populations in the Alsace (France) represent one cluster, together with the Swiss populations near Basel, which is separated by the river Rhine from the cluster composed of the populations in southwestern Germany. Thus, the historical separation by the river Rhine surpasses more recent effects of human-induced habitat fragmentation on the genetic differentiation in I. fuliginator.
Osmia (Melanosmia) uncinata Gerstäcker is a Palaearctic megachilid bee distributed from temperate and northern Europe eastwards to the Russian Far East. The discovery of over 80 nests in Switzerland, ...southern Germany and Scotland enabled for the first time a closer investigation of its nesting biology and prompted the assessment of the species’ phenology, distribution and habitat.
O. uncinata
nested in self-excavated burrows inside the bark of both living trunks and dead stumps of
Pinus sylvestris
. The nests were excavated at a height of 10–220 cm above ground either on the underside of prominences of longitudinal bark ribs or inside beetle borings and extended more or less vertically upwards. They consisted of a single straight to slightly curved burrow with rarely one to three side burrows, had a total length of 1.2–12.0 cm and contained 1–6 brood cells. The brood cells, which faced downwards with the larval provisions being located in the upper cell half, were separated from each other by one-layered walls of chewed leaves (“leaf pulp”). The nests were sealed with a plug of 2–4 closely adjacent walls of leaf pulp. DNA metabarcoding of cell and plug walls revealed that
Potentilla
and
Fragaria
(Rosaceae) served as leaf pulp sources. Pre-imaginal mortality amounted to 77%, partly caused by brood parasites such as
Sapyga similis
(Sapygidae) and
Cacoxenus indagator
(Drosophilidae) or predators such as snakeflies (Raphidioptera). At low elevations,
O. uncinata
needs one year for its development and overwinters as imago inside the nest, whereas in the subalpine zone of the Alps it has a two-year cycle passing the first winter as prepupa and the second winter as imago.
O. uncinata
starts to emerge between the end of March at low elevations and the end of May at higher elevations qualifying as an early flying bee like the other European O. (Melanosmia) species. The distribution of
O. uncinata
in Central Europe and Scotland largely coincides with the occurrence of
P. sylvestris
. As in the pine, it extends over a wide altitudinal range from below 100 m up to 1900 m a.s.l. and encompasses dry and wet as well as warm and cold habitats including open pine forests, inner and outer forest edges dominated by pine and isolated pine groups. At a few locations in the subalpine zone of the Alps,
O. uncinata
occurs in the absence of
P. sylvestris
; here, the thick bark of
Larix decidua
serves as a substitute nesting substrate.
The sampling of living insects should be avoided in highly endangered species when the sampling would further increase the risk of population extinction. Nonlethal sampling (wing clips or leg ...removals) can be an alternative to obtain DNA of individuals for population genetic studies. However, nonlethal sampling may not be possible for all insect species. We examined whether remnants of traffic-killed specimens of the endangered and protected flightless longhorn beetle Iberodorcadion fuliginator (L., 1758) can be used as a resource for population genetic analyses. Using insect fragments of traffic-killed specimens collected over 15 yr, we determined the most efficient DNA extraction method in relation to the state of the specimens (crushed, fragment, or intact), preservation (dried, airtight, or in ethanol), storage duration, and weight of the sample by assessing the quantity and quality of genomic DNA. A modified cetyltrimethyl ammonium bromide method provided the highest recovery rate of genomic DNA and the largest yield and highest quality of DNA. We further used traffic-killed specimens to evaluate two DNA amplification techniques (quantitative polymerase chain reaction qPCR and microsatellites). Both qPCR and microsatellites revealed successful DNA amplification in all degraded specimens or beetle fragments examined. However, relative qPCR concentration and peak height of microsatellites were affected by the state of specimen and storage duration but not by specimen weight. Our investigation demonstrates that degraded remnants of traffic-killed beetle specimens can serve as a source of high-quality genomic DNA, which allows to address conservation genetic issues.
Old herbarium specimens and historical floristic data give insight into regional floras for given time periods. They often cover historical time periods for which few other data are available. ...Herbaria thus allow the study of changes of the flora of a region across time. Using a 150-year-old regional herbarium together with a historical publication, we investigated to which extent the flora of the Swiss canton of Schaffhausen has changed, which habitats were particularly affected by local extinction, what the environmental requirements of extinct plants were and whether conclusions about the respective drivers such as land use change can be drawn. A total of 987 species were historically recorded in the study region of which 154 are currently no longer reported and are regionally extinct. This means that about one species disappeared from the region every year. Species that are currently in a high category of endangerment on the Swiss Red List have declined markedly in the canton of Schaffhausen, showing that Red Lists well portrait the endangerment of species. Looking at plant strategies, the more stress-tolerant and less competitive plants have disappeared. In addition, wetland, pioneer, ruderal and mountain species as well as agricultural weeds and light-demanding species showed highest extinction rates. In contrast, forest species had a low extinction rate, and species from fertilized meadows showed no decline. Our evaluation of a regional herbarium helps to inform nature conservation about particularly endangered habitats and possible drivers of species decline.
•Characterization of fly ash and filter cake of three different large scale fly ash leaching processes.•Systematic description of metal associations responsible for mobilization of metals during fly ...ash leaching.•Factors controlling metal mobilization are pH-value, redox conditions, liquid-to-solid-ratio, temperature and reaction time.•Hydrogen peroxide addition results in a significant increase of Pb and Cu depletion factor in filter cake.•The study delivers valuable basis for definition of state of the art fly ash treatment in Switzerland.
This study focusses on chemical and mineralogical characterization of fly ash and leached filter cake and on the determination of parameters influencing metal mobilization by leaching. Three different leaching processes of fly ash from municipal solid waste incineration (MSWI) plants in Switzerland comprise neutral, acidic and optimized acidic (+ oxidizing agent) fly ash leaching have been investigated. Fly ash is characterized by refractory particles (Al-foil, unburnt carbon, quartz, feldspar) and newly formed high-temperature phases (glass, gehlenite, wollastonite) surrounded by characteristic dust rims. Metals are carried along with the flue gas (Fe-oxides, brass) and are enriched in mineral aggregates (quartz, feldspar, wollastonite, glass) or vaporized and condensed as chlorides or sulphates. Parameters controlling the mobilization of neutral and acidic fly ash leaching are pH and redox conditions, liquid to solid ratio, extraction time and temperature. Almost no depletion for Zn, Pb, Cu and Cd is achieved by performing neutral leaching. Acidic fly ash leaching results in depletion factors of 40% for Zn, 53% for Cd, 8% for Pb and 6% for Cu. The extraction of Pb and Cu are mainly limited due to a cementation process and the formation of a PbCu0-alloy-phase and to a minor degree due to secondary precipitation (PbCl2). The addition of hydrogen peroxide during acidic fly ash leaching (optimized acidic leaching) prevents this reduction through oxidation of metallic components and thus significantly higher depletion factors for Pb (57%), Cu (30%) and Cd (92%) are achieved. The elevated metal depletion using acidic leaching in combination with hydrogen peroxide justifies the extra effort not only by reduced metal loads to the environment but also by reduced deposition costs.
•Laboratory study of fly ash leaching using HCl 5% and NaCl-solution (300 g/L).•Mobilization of Pb and Cu mainly dependent from redox conditions and metal-chloride-complex formation.•Thermodynamic ...modelling used to determine speciation of Pb, Cu, Zn and Cd in leaching solutions.•Results of this study indicate a way forward for an improved metal depletion and recovery from fly ash.
Fly ash from municipal solid waste incineration contains a large potential for recyclable metals such as Zn, Pb, Cu and Cd. The Swiss Waste Ordinance prescribes the treatment of fly ash and recovery of metals to be implemented by 2021. More than 60% of the fly ash in Switzerland is acid leached according to the FLUWA process, which provides the basis for metal recovery. The investigation and optimization of the FLUWA process is of increasing interest and an industrial solution for direct metal recovery within Switzerland is in development. With this work, a detailed laboratory study on different filter cakes from fly ash leaching using HCl 5% (represents the FLUWA process) and concentrated sodium chloride solution (300 g/L) is described. This two-step leaching of fly ash is an efficient combination for the mobilization of a high percentage of heavy metals from fly ash (Pb, Cd ≥ 90% and Cu, Zn 70–80%). The depletion of these metals is mainly due to a combination of redox reaction and metal-chloride-complex formation. The results indicate a way forward for an improved metal depletion and recovery from fly ash that has potential for application at industrial scale.
In recent years, complex new bottom ash treatment processes for enhanced metal recovery have been implemented in Switzerland, producing residual bottom ash fractions with various qualities. This ...study focusses on three different treatment processes by characterizing all arising fractions in detail. Thereby the factors influencing the composition of these fractions are identified and their recycling potential in Switzerland is investigated. However, high legislative requirements on total contents of heavy metals represent a high barrier for bottom ash recycling in Switzerland. Therefore, the recycling potential is further evaluated based on the waste legislation applied in the Netherlands, where recycling of bottom ash has a long tradition. There, threshold values for bottom ash recycling are based on leachate concentrations and not on total contents as in Switzerland. However, Swiss Waste Legislation also knows threshold values based on leachate concentrations for certain waste materials. The leaching tests applied in these two countries, however, are different. The comparison of both leaching tests reveals that the setup and conditions, especially the considered pH range, significantly influence the leaching of heavy metals. With emphasis on problematic pollutants, the possibilities for new applications of these fractions are evaluated based on Swiss and Dutch legal threshold values. The comparison within the legal frameworks of these two countries allows recognizing opportunities and risks related to bottom ash recycling.