Summary
Loop‐mediated isothermal amplification (LAMP) amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions by using a DNA polymerase with high displacement strand ...activity and a set of specifically designed primers to amplify targeted DNA strands. Following its first discovery by Notomi et al. ( Nucleic Acids Res 28: E63), LAMP was further developed over the years which involved the combination of this technique with other molecular approaches, such as reverse transcription and multiplex amplification for the detection of infectious diseases caused by micro‐organisms in humans, livestock and plants. In this review, available types of LAMP techniques will be discussed together with their applications in detection of various micro‐organisms. Up to date, there are varieties of LAMP detection methods available including colorimetric and fluorescent detection, real‐time monitoring using turbidity metre and detection using lateral flow device which will also be highlighted in this review. Apart from that, commercialization of LAMP technique had also been reported such as lyophilized form of LAMP reagents kit and LAMP primer sets for detection of pathogenic micro‐organisms. On top of that, advantages and limitations of this molecular detection method are also described together with its future potential as a diagnostic method for infectious disease.
This study engaged health professionals in in-depth, semi-structured interviews to explore their opinions concerning the issues surrounding vaccine hesitancy in Malaysia and strategies to improve ...vaccination to stamp the rise of vaccine preventable diseases (VPDs). Opinions on how to address the resurgence of VPDs in the era of increasing vaccine hesitancy were obtained. Eight health professionals, including geriatricians, pediatricians, microbiologists, public health specialists, and family medicine specialists were interviewed. The influence of anti-vaccination propaganda, past-experience of adverse event following immunization (AEFI), perceived religious prohibition, a belief that traditional complementary and alternative medicine (TCAM) use is safer, pseudoscience beliefs, and anti-vaccine conspiracy theories were identified as reasons for refusing to vaccinate. The interplay of social, cultural and religious perspectives in influencing perceived religious prohibition, pseudoscience beliefs, and the use of TCAM contributing to vaccine refusal was found. Five broad themes emerged from the health professionals regarding strategies to address vaccine hesitancy, including establishing an electronic vaccination registry, increasing public awareness initiatives, providing feedback to the public on the findings of AEFI, training of front-line healthcare providers, and banning the dissemination of anti-vaccine information via social media. With regards to identifying strategies to address the resurgence of VPDs, mandatory vaccination received mixed opinions; many viewed supplementary immunization activity and the prevention of travel and migration of unvaccinated individuals as being necessary. In conclusion, the present study identified unique local cultural, traditional and religious beliefs that could contribute to vaccine hesitancy in addition to issues surrounding vaccination refusal similarly faced by other countries around the world. This information are important for the formulation of targeted intervention strategies to stamp vaccine hesitancy in Malaysia which are also a useful guide for other countries especially in the Southeast Asia region facing similar vaccine hesitancy issues.
The Efficacy of Violence Prediction Yang, Min; Wong, Stephen C. P; Coid, Jeremy
Psychological bulletin,
09/2010, Letnik:
136, Številka:
5
Journal Article
Recenzirano
Actuarial risk assessment tools are used extensively to predict future violence, but previous studies comparing their predictive accuracies have produced inconsistent findings as a result of various ...methodological issues. We conducted meta-analyses of the effect sizes of 9 commonly used risk assessment tools and their subscales to compare their predictive efficacies for violence. The effect sizes were extracted from 28 original reports published between 1999 and 2008, which assessed the predictive accuracy of more than one tool. We used a within-subject design to improve statistical power and multilevel regression models to disentangle random effects of variation between studies and tools and to adjust for study features. All 9 tools and their subscales predicted violence at about the same moderate level of predictive efficacy with the exception of Psychopathy Checklist-Revised (PCL-R) Factor 1, which predicted violence only at chance level among men. Approximately 25% of the total variance was due to differences between tools, whereas approximately 85% of heterogeneity between studies was explained by methodological features (age, length of follow-up, different types of violent outcome, sex, and sex-related interactions). Sex-differentiated efficacy was found for a small number of the tools. If the intention is only to predict future violence, then the 9 tools are essentially interchangeable; the selection of which tool to use in practice should depend on what other functions the tool can perform rather than on its efficacy in predicting violence. The moderate level of predictive accuracy of these tools suggests that they should not be used solely for some criminal justice decision making that requires a very high level of accuracy such as preventive detention.
Brown carbon (BrC) consists of those organic compounds in atmospheric aerosols that absorb solar radiation and may play an important role in planetary radiative forcing and climate. However, little ...is known about the production and loss mechanisms of BrC in the atmosphere. Here, we study how the light absorptivity of BrC from wood smoke and secondary BrC generated from the reaction of ammonium sulfate with methylglyoxal changes under photolytic aging by UVA radiation in the aqueous phase. Owing to its chemical complexity, BrC is separated by molecular weight using size exclusion chromatography, and the response of each molecular weight fraction to aging is studied. Photolytic aging induced significant changes in the light absorptivity of BrC for all molecular weight fractions; secondary BrC was rapidly photoblenched, whereas for wood smoke BrC, both photoenhancement and photobleaching were observed. Initially, large biomass burning BrC molecules were rapidly photoenhanced, followed by slow photolysis. As a result, large BrC molecules dominated the total light absorption of aged biomass burning BrC. These experimental results further support earlier observations that large molecular weight BrC compounds from biomass burning can be relatively long-lived components in atmospheric aerosols, thus more likely to have larger impacts on aerosol radiative forcing and could serve as biomass burning tracers.
The real‐time control of urban watersheds is now being enabled by a new generation of “smart” and connected technologies. By retrofitting stormwater systems with sensors and valves, it becomes ...possible to adapt entire watersheds dynamically to individual storms. A catchment‐scale control algorithm is introduced, which s an urban watershed as a linear integrator delay dynamical system, parameterizes it using physical watershed characteristics, and then controls network flows using a Linear Quadratic Regulator. The approach is simulated on a 4‐km2 urban headwater catchment in Ann Arbor, Michigan, demonstrating the gains of a stormwater system that can adaptively balance between flood mitigation and flow reduction. We introduce an equivalence analysis and illustrate the performance of the controlled watershed across large events (30‐year storms) to show the uncontrolled passive watershed can only match it during smaller events (10‐year storm). For these smaller events, the storage volume of the controlled storage nodes (ponds, basins, and wetlands) could be reduced as much as 50% and still achieve the same performance of the controlled watershed. A controller placement analysis is also carried out, whereby all possible combinations of controlled sites are simulated across a wide spectrum of design storms. We show that the control of every storage node may not be needed in a watershed, but rather that in our case study a small subset (30%) of the overall watershed can be controlled in coordination to achieve outcomes that match a fully controlled system, even when tested across a long‐term rainfall record and under noisy sensor measurements.
Plain Language Summary
Internet‐connected sensors are changing how we study and manage water systems. By adding valves, gates, and pumps to stormwater systems, it will be possible to adaptively control urban watershed in response to individual storms. Without requiring new and expensive construction, this will allow existing infrastructure to be used more effectively. In this paper, we introduce a control algorithm that can be used to control urban watersheds. We also investigate how many control valves are needed to retrofit an urban watershed and where, which may serve as tools for city managers to reduce flooding and manage flows.
Key Points
A linear feedback controller is formulated for the control of urban catchments (1‐5 km2) using physical catchment properties
Simulations show strong flow control performance when compared to uncontrolled catchment
Desired hydraulic outcomes can be achieved by retrofitting a small number of control sites
Ecosystems services (ES) assessment is a significant scientific topic recognized for its potential to address sustainability issues. However, there is an absence of science-policy frameworks in land ...use planning that lead to the ES science being used in policy. China's Ecological Redline Policy (ERP) is one of the first national policies utilizing multiple ES, but there is no standardized approach for working across the science-policy interface. We propose a transdisciplinary framework to determine ecological redline areas (ERAs) in Shanghai using: ES, biodiversity and ecologically fragile hotspots, landscape structure, and stakeholder opinions. We determine the five criteria to identify ERAs for Shanghai using multi-temporal, high resolution images (0.5 m) and biophysical models. We examine ERP effectiveness by comparing land use scenarios for 2040. Compared to alternative land uses, ES increase significantly under the ERP. The inclusion of ES in spatial planning led stakeholders to increase terrestrial habitat protection by 174% in Shanghai. Our analysis suggests that strategic planning for ES could reduce tradeoffs between environmental quality and development.
We present a data-calibrated compact model of carbon nanotube (CNT) FETs (CNTFETs) based on the virtual-source (VS) approach, describing the intrinsic current-voltage and charge-voltage ...characteristics. The features of the model include: 1) carrier VS velocity extracted from experimental devices with gate lengths down to 15 nm; 2) carrier effective mobility and velocity depending on the CNT diameter; 3) short channel effect such as inverse subthreshold slope degradation and drain-induced barrier lowering depending on the device dimensions; and 4) small-signal capacitances including the CNT quantum capacitance effect to account for the decreasing gate capacitance at high gate bias. The CNTFET model captures the dimensional scaling effects and is suitable for technology benchmarking and performance projection at the sub-10-nm technology nodes.
A key mechanism for atmospheric secondary organic aerosol (SOA) formation occurs when oxidation products of volatile organic compounds condense onto pre-existing particles. Here, we examine effects ...of aerosol liquid water (ALW) on relative SOA yield and composition from α-pinene ozonolysis and the photooxidation of toluene and acetylene by OH. Reactions were conducted in a room-temperature flow tube under low-NO x conditions in the presence of equivalent loadings of deliquesced (∼20 μg m–3 ALW) or effloresced (∼0.2 μg m–3 ALW) ammonium sulfate seeds at exactly the same relative humidity (RH = 70%) and state of wall conditioning. We found 13% and 19% enhancements in relative SOA yield for the α-pinene and toluene systems, respectively, when seeds were deliquesced rather than effloresced. The relative yield doubled in the acetylene system, and this enhancement was partially reversible upon drying the prepared SOA, which reduced the yield by 40% within a time scale of seconds. We attribute the high relative yield of acetylene SOA on deliquesced seeds to aqueous partitioning and particle-phase reactions of the photooxidation product glyoxal. The observed range of relative yields for α-pinene, toluene, and acetylene SOA on deliquesced and effloresced seeds suggests that ALW plays a complicated, system-dependent role in SOA formation.
One of the long-standing problems in antenna engineering is the realization of highly directive beams using low-profile devices. In this paper, we provide a solution to this problem by means of ...Huygens' metasurfaces (HMSs), based on the equivalence principle. This principle states that a given excitation can be transformed to a desirable aperture field by inducing suitable electric and (equivalent) magnetic surface currents. Building on this concept, we propose and demonstrate cavity-excited HMS antennas, where the single-source-fed cavity is designed to optimize aperture illumination, while the HMS facilitates the current distribution that ensures phase purity of aperture fields. The HMS breaks the coupling between the excitation and radiation spectra typical to standard partially reflecting surfaces, allowing tailoring of the aperture properties to produce a desirable radiation pattern, without incurring edge-taper losses. The proposed low-profile design yields near-unity aperture illumination efficiencies from arbitrarily large apertures, offering new capabilities for microwave, terahertz and optical radiators.
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