As the core components of abscisic acid (ABA) signal pathway, Clade A PP2C (PP2C-A) phosphatases in ABA-dependent stress responses have been well studied in
. However, the roles and natural ...variations of maize PP2C-A in stress responses remain largely unknown. In this study, we investigated the expression patterns of
treated with multiple stresses and generated transgenic
plants overexpressing most of the
genes. The results showed that the expression of most
were dramatically induced by multiple stresses (drought, salt, and ABA), indicating that these genes may have important roles in response to these stresses. Compared with wild-type plants,
,
, and
overexpression plants had higher germination rates after ABA and NaCl treatments.
and
negatively regulated drought responses as the plants overexpressing these genes had lower survival rates, higher leaf water loss rates, and lower proline accumulation compared to wild type plants. The natural variations of
associated with drought tolerance were also analyzed and favorable alleles were detected. We widely studied the roles of
genes in stress responses and the natural variations detected in these genes have the potential to be used as molecular markers in genetic improvement of maize drought tolerance.
This paper reviews publications in the literature on performance degradation of and mitigation strategies for polymer electrolyte membrane (PEM) fuel cells. Durability is one of the characteristics ...most necessary for PEM fuel cells to be accepted as a viable product. In this paper, a literature-based analysis has been carried out in an attempt to achieve a unified definition of PEM fuel cell lifetime for cells operated either at a steady state or at various accelerated conditions. Additionally, the dependence of PEM fuel cell durability on different operating conditions is analyzed. Durability studies of the individual components of a PEM fuel cell are introduced, and various degradation mechanisms are examined. Following this analysis, the emphasis of this review shifts to applicable strategies for alleviating the degradation rate of each component. The lifetime of a PEM fuel cell as a function of operating conditions, component materials, and degradation mechanisms is then established. Lastly, this paper summarizes accelerated stress testing methods and protocols for various components, in an attempt to prevent the prolonged test periods and high costs associated with real lifetime tests.
Drought is a major environmental disaster that causes crop yield loss worldwide. Metabolites are involved in various environmental stress responses of plants. However, the genetic control of ...metabolomes underlying crop environmental stress adaptation remains elusive.
Here, we perform non-targeted metabolic profiling of leaves for 385 maize natural inbred lines grown under well-watered as well as drought-stressed conditions. A total of 3890 metabolites are identified and 1035 of these are differentially produced between well-watered and drought-stressed conditions, representing effective indicators of maize drought response and tolerance. Genetic dissections reveal the associations between these metabolites and thousands of single-nucleotide polymorphisms (SNPs), which represented 3415 metabolite quantitative trait loci (mQTLs) and 2589 candidate genes. 78.6% of mQTLs (2684/3415) are novel drought-responsive QTLs. The regulatory variants that control the expression of the candidate genes are revealed by expression QTL (eQTL) analysis of the transcriptomes of leaves from 197 maize natural inbred lines. Integrated metabolic and transcriptomic assays identify dozens of environment-specific hub genes and their gene-metabolite regulatory networks. Comprehensive genetic and molecular studies reveal the roles and mechanisms of two hub genes, Bx12 and ZmGLK44, in regulating maize metabolite biosynthesis and drought tolerance.
Our studies reveal the first population-level metabolomes in crop drought response and uncover the natural variations and genetic control of these metabolomes underlying crop drought adaptation, demonstrating that multi-omics is a powerful strategy to dissect the genetic mechanisms of crop complex traits.
After germination, seedlings undergo growth arrest in response to unfavourable conditions, a critical adaptation enabling plants to survive harsh environments. The plant hormone abscisic acid (ABA) ...plays a key role in this arrest. To arrest growth, ABA‐dependent transcription factors change gene expression patterns in a flexible and reversible manner. Although the control of gene expression has important roles in growth arrest, the epigenetic mechanisms in the response to ABA are not fully understood. Here, we show that the histone demethylases JUMONJI‐C domain‐containing protein 30 (JMJ30) and JMJ32 control ABA‐mediated growth arrest in Arabidopsis thaliana. During the postgermination stage (2–3 days after germination), the ABA‐dependent transcription factor ABA‐insensitive3 (ABI3) activates the expression of JMJ30 in response to ABA. JMJ30 then removes a repressive histone mark, H3 lysine 27 trimethylation (H3K27me3), from the SNF1‐related protein kinase 2.8 (SnRK2.8) promoter, and hence activates SnRK2.8 expression. SnRK2.8 encodes a kinase that activates ABI3 and is responsible for JMJ30‐ and JMJ32‐mediated growth arrest. A feed‐forward loop involving the ABI3 transcription factor, JMJ histone demethylases, and the SnRK2.8 kinase fine‐tunes ABA‐dependent growth arrest in the postgermination phase. Our findings highlight the importance of the histone demethylases in mediating adaptation of plants to the environment.
The histone demethylases JMJ30 and JMJ32 regulate the tolerance programme for ABA‐mediated growth arrest in Arabidopsis.
•Fire-induced hydrological impacts were assessed using the OpenLISEM model.•Rainfall dominated the catchment hydro-sedimentary responses before and after fire.•Fire led to a mean increase of 57% in ...peak discharge and up to 31% in sediment yield.•Sediment sources partly changed from croplands to burnt areas.
Wildfires can have strong negative effects on soil and water resources, especially in headwater areas. The spatially explicit OpenLISEM model was applied to a burned catchment in southern Portugal to quantify the individual and combined impacts of wildfire and rainfall on hydrological and erosion processes. The companion paper has calibrated and assessed model performance in this area before and after a fire. In this study, the model was applied with design storms of six different return periods (0.2, 0.5, 1, 2, 5, and 10 years) to simulate and evaluate pre- and post-wildfire hydrological and erosion responses at the catchment scale. Our results show that rainfall amount and intensity played a more important role than fire occurrence in the catchment discharge and sediment yields. Fire occurrence was found to be an important factor for peak discharge, indicating that high post-fire hydro-sedimentary responses are frequently related to extreme rainfall events. The results also suggest a partial shift from runoff to splash erosion after fire, especially for higher return periods. This can be explained by increased splash erosion in burned upstream areas saturating the sediment transport capacity of surface runoff, limiting runoff erosion in downstream areas. Therefore, the pre-fire erosion risk in the croplands of this catchment was partly shifted to a post-fire erosion risk in upper slope forest and natural areas, especially for storms with lower return periods, although erosion risks in croplands were important both before and after fires. These findings have significant implications to identify areas for post-wildfire stabilization and rehabilitation, which is particularly important given the predicted increase in the occurrence of fires and extreme rainfall events with climate change.
Colon adenocarcinoma (COAD) represents a type of common malignant tumor originating in the digestive tract. Long non-coding RNAs (lncRNAs) have been identified to engage in regulating the initiation ...and development of COAD. LncRNA LINC02253 has been reported abnormal expressed in COAD, but the underlying mechanism has not been discussed so far. This study aimed to determine the role and the molecular biology mechanism of LINC02253 in COAD progression and unearthed its specific molecular mechanism.
RT-qPCR and Western blot assays were conducted to detect gene expression. Function assays were performed to evaluate the effect of gene expression on COAD cell phenotype. Mechanism analyses were done to verify the association among genes after bioinformatics analysis. The obtained data revealed that LINC02253 demonstrated a high expression in COAD tissues and cells. This gene served as an oncogene, permitting to stimulate proliferation and suppress apoptosis of COAD cells. Mechanically, it was found that LINC02253 recruited FUS to stabilize WWP1 mRNA and WWP1 could mediate SMAD3 ubiquitination, thereby promoting the malignant phenotype formation of COAD cells.
LINC02253 was uncovered to exert an oncogenic role, enhancing the proliferation of COAD cells and repressing the cell apoptosis by recruiting FUS and encouraging WWP1-mediated SMAD3 ubiquitination.
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•This study first verified that LINC02253 could promote proliferation of COAD cells but suppress cell apoptosis.•This study first revealed that LINC02253 recruited FUS to stabilize WWP1 mRNA.•This study first confirmed that WWP1 regulated SMAD3 ubiquitination to promote COAD cell growth.
•We studied the reliability of individual similarity in brain responses to movies.•We obtained voxel-wise and parcel-wise maps of test–retest reliability measures.•1/3 voxels reflected reliable ...individual similarity in an emotion-evoking movie.•Some regions exhibited “trait-like” individual similarity and reliability.•The results support applying naturalistic imaging to individualized study.
Understanding individual differences in brain function is an essential aim of neuroscience. Naturalistic imaging links neural activity to real-life contexts and reflects individual differences in brain response. These unique features make it a promising tool for individualized psychiatry. An essential prerequisite for the extensive use of this paradigm is the reliable representation of inter-individual relationships. We used a test–retest approach to examine whether the naturalistic paradigm reliably represents inter-individual differences, which brain regions have the superior capability, and whether the ability alters with the contents of the stimuli. We quantified the reliability of the inter-subject relationships in repeated scans of two movie clips: a natural sight view and an emotion-evoking story. Besides statistical inference, we included resting-state scans, behavioral tests, and questionnaires as references for the comparison. The results showed that over one-third area of the brain could reliably characterize the inter-individual relationship, and the superior temporal lobe demonstrated comparable reliability representation with the State and Trait Anxiety Inventory. Furthermore, the temporal lobe regions could retain this capability across emotional movies with different contents. This study provides a base for pushing the naturalistic imaging paradigm towards clinical applications and proposes reliable target brain regions for future studies.
Seed germination is an important development process in plant growth. The phytohormone abscisic acid (ABA) plays a critical role during seed germination. However, the mechanism of rapeseed in ...response to ABA is still elusive. In order to understand changes of rapeseed under exogenous ABA treatment, we explored differentially expressed metabolites (DEMs) and the differentially expressed genes (DEGs) between mock- and ABA-treated seedlings. A widely targeted LC-MS/MS based metabolomics were used to identify and quantify metabolic changes in response to ABA during seed germination, and a total of 186 significantly DEMs were identified. There are many compounds which are involved in ABA stimuli, especially some specific ABA transportation-related metabolites such as starches and lipids were screened out. Meanwhile, a total of 4440 significantly DEGs were identified by transcriptomic analyses. There was a significant enrichment of DEGs related to phenylpropanoid and cell wall organization. It suggests that exogenous ABA mainly affects seed germination by regulating cell wall loosening. Finally, the correlation analysis of the key DEMs and DEGs indicates that many DEGs play a direct or indirect regulatory role in DEMs metabolism. The integrative analysis between DEGs and DEMs suggests that the starch and sucrose pathways were the key pathway in ABA responses. The two metabolites from starch and sucrose pathways, levan and cellobiose, both were found significantly down-regulated in ABA-treated seedlings. These comprehensive metabolic and transcript analyses provide useful information for the subsequent post-transcriptional modification and post germination growth of rapeseed in response to ABA signals and stresses.
Modified BuShenYiQi formula (M-BYF) is derived from BuShenYiQi formula, used for the treatment of allergic asthma. The exact effect and mechanism of M-BYF on the improvement of asthma remain unclear.
...We investigated the mechanism underlying the therapeutic effect of M-BYF on allergic asthma.
The asthma model was established in female BALB/c mice that were sensitized and challenged with ovalbumin (OVA). Mice in the treated groups were orally treated once a day with M-BYF (7, 14 and 28 g/kg/d) or dexamethasone before OVA challenge. Control and Model group received saline. Pathophysiological abnormalities and percentages of lung type 2 innate lymphoid cells (ILC2s) and Th9 cells were measured. Expression levels of type 2 cytokines and transcription factors required for these cells function and differentiation were analysed. Expression of vasoactive intestinal polypeptide (VIP)-VPAC2 signalling pathway-related proteins, and percentages of VIP expressing (VIP
+
) cells and VPAC2, CD90 co-expressing (VPAC2
+
CD90
+
) cells were detected.
M-BYF alleviated airway hyperresponsiveness, inflammation, mucus hypersecretion and collagen deposition in asthmatic mice. M-BYF down-regulated percentages of ILC2s and Th9 cells with lower expression of GATA3, PU.1 and IRF4, reduced IL-5, IL-13, IL-9 and VIP production. The decrease in the expression of VIP-VPAC2 signalling pathway and percentages of VIP
+
cells, VPAC2
+
CD90
+
cells were observed after M-BYF treatment. The LD
50
value of M-BYF was higher than 90 g/kg.
M-BYF alleviated experimental asthma by negatively regulating ILC2s and Th9 cells and the VIP-VPAC2 signalling pathway. These findings provide the theoretical basis for future research of M-BYF in asthma patient population.
Sex hormones are steroid hormones synthesized from the gonads of animals and tissues such as the placenta and adrenocortical reticular zone. The physiological functions of sex hormones are complex. ...Sex hormones are not only pathologically correlated with many diseases of the reproductive system, but are etiological factors in some viral infectious diseases, including disease caused by infections of coronaviruses, herpesviruses, hepatitis viruses, and other kinds of human viruses, which either exhibit a male propensity in clinical practice, or crosstalk with androgen receptor (AR)-related pathways in viral pathogenesis. Due to the global pandemic of coronavirus disease 2019 (COVID-19), the role of androgen/AR in viral infectious disease is highlighted again, majorly representing by the recent advances of AR-responsive gene of transmembrane protease/serine subfamily member 2 (TMPRSS2), which proteolytically activates the receptor-mediated virus entry by many coronaviruses and influenza virus, along with the role of androgen-mediated signaling for the transcription of hepatitis B virus (HBV), and the role of sex hormone responsive genes during Zika virus (ZIKV) pathogenesis, et al. Collectively, we propose to provide a comprehensive overview of the role of male sex hormones during multiple phases in the life cycle of different human viruses, which may be partly responsible for the sex-specific prevalence, severity and mortality of some diseases, therefore, may provide clues to develop more efficient prevention and treatment strategies for high-risk populations.
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