N6-methyladenosine (m
A) and DNA 5-methylcytosine (5mC) methylation plays crucial roles in diverse biological processes, including skeletal muscle development and growth. Recent studies unveiled a ...potential link between these two systems, implicating the potential mechanism of coordinated transcriptional and post-transcriptional regulation in porcine prenatal myogenesis and postnatal skeletal muscle growth.
Immunofluorescence and co-IP assays were carried out between the 5mC writers and m
A writers to investigate the molecular basis underneath. Large-scale in-house transcriptomic data were compiled for applying weighted correlation network analysis (WGCNA) to identify the co-expression patterns of m
A and 5mC regulators and their potential role in pig myogenesis. Whole-genome bisulfite sequencing (WGBS) and methylated RNA immunoprecipitation sequencing (MeRIP-seq) were performed on the skeletal muscle samples from Landrace pigs at four postnatal growth stages (days 30, 60, 120 and 180).
Significantly correlated expression between 5mC writers and m
A writers and co-occurrence of 5mC and m
A modification were revealed from public datasets of C2C12 myoblasts. The protein-protein interactions between the DNA methylase and the m
A methylase were observed in mouse myoblast cells. Further, by analyzing transcriptome data comprising 81 pig skeletal muscle samples across 27 developmental stages, we identified a 5mC/m
A epigenetic module eigengene and decoded its potential functions in pre- or post-transcriptional regulation in postnatal skeletal muscle development and growth of pigs. Following integrative multi-omics analyses on the WGBS methylome data and MeRIP-seq data for both m
A and gene expression profiles revealed a genome/transcriptome-wide correlated dynamics and co-occurrence of 5mC and m
A modifications as a consequence of 5mC/m
A crosstalk in the postnatal myogenesis progress of pigs. Last, we identified a group of myogenesis-related genes collaboratively regulated by both 5mC and m
A modifications in postnatal skeletal muscle growth in pigs.
Our study discloses a potential epigenetic mechanism in skeletal muscle development and provides a novel direction for animal breeding and drug development of related human muscle-related diseases.
Phytochromes (phy) are distributed in various plant organs, and their physiological effects influence plant germination, flowering, fruiting, and senescence, as well as regulate morphogenesis ...throughout the plant life cycle. Reactive oxygen species (ROS) are a key regulatory factor in plant systemic responses to environmental stimuli, with an attractive regulatory relationship with phytochromes. With the development of high-throughput sequencing technology, omics techniques have become powerful tools, and researchers have used omics techniques to facilitate the big data revolution. For an in-depth analysis of phytochrome-mediated signaling pathways, integrated multi-omics (transcriptomics, proteomics, and metabolomics) approaches may provide the answer from a global perspective. This article comprehensively elaborates on applying multi-omics techniques in studying phytochromes. We describe the current research status and future directions on transcriptome-, proteome-, and metabolome-related network components mediated by phytochromes when cells are subjected to various stimulation. We emphasize the importance of multi-omics technologies in exploring the effects of phytochromes on cells and their molecular mechanisms. Additionally, we provide methods and ideas for future crop improvement.
The antral follicle stage is a critical period in mammalian oocyte maturation, marked by complex interactions between oocyte development and neighboring granulosa cells. Understanding the ...heterogeneity and communication patterns of granulosa cells within antral follicles is crucial for deciphering their roles in follicle development and oocyte maturation. Here, we employed single-cell RNA-sequencing to explore the molecular and cellular characteristics of porcine antral follicles. Our analysis revealed distinct subpopulations within mural and cumulus granulosa cells, indicating diverse cellular states and functions within the follicles. Functional enrichment analysis unveiled the involvement of specific subpopulations in steroid biosynthesis, cumulus expansion, and cellular communication. Moreover, comparing mature and less mature follicles highlighted differences in cell distribution and functions, indicating developmental-specific variations. Our findings shed light on the intricate cellular heterogeneity and communication network within porcine antral follicles, providing valuable insights into the regulation of follicle development and oocyte maturation in pigs. These results hold promise for improving pig reproductive efficiency and advancing human reproductive medicine.
With the rapid development of the economy, the integration of corporate strategic management and human resource management has become an issue of concern. This research mainly discusses the role of ...intelligent communication management terminal in the construction of human resource management mode. In this research, the system development process of this research mainly uses the class library in the software architecture layer to support the software development process. The main development language of Android, JAVA, is to install the Android Develop Tools plug-in on eclipse and install the Android SDK in the computer operating system to build the Android development environment. The development and application of the system not only make the enterprise managers more convenient and efficient in the process of managing the enterprise but also smooth the operation of the enterprise while reducing the human resource investment and also gives the employees more right to know and the right to participate in the enterprise construction. By creating more value while reducing human resource input, enterprises will enable it to obtain more benefits, and thus enter a cycle of good development and contribute to society. The system has the functions of personnel management, recruitment management, attendance management, training management, work management, and salary management. The recruitment management function of the system is mainly composed of recruitment plan management, recruitment information management, and talent pool. In the system’s recruitment plan management function, important information such as the recruitment part, the number of recruits, personnel requirements, and the specific arrival time of the personnel must be clarified. The personnel in charge of the enterprise personnel department shall conduct corresponding regulations according to the specific needs of the enterprise and shall be experienced by the personnel department. The review is carried out, and all parts of the enterprise are coordinated and completed at the same time. In the platform performance test, when the number of concurrent users reaches 50000, the request time is about 6 seconds, which meets the requirement that the response time of 10000 people per second is less than 10 seconds. This research puts forward suggestions and countermeasures for the optimization of human resource management, which can not only improve the efficiency of Y company’s human resource management but also provide useful reference and reference for other enterprises facing the same problem.
In response to the application requirement of wide-range high-resolution imaging of non-cooperative moving real targets by inverse synthetic-aperture ladar (ISAL), experiments were conducted on the ...depolarization effect of target materials, and the polarization selection of ISAL receiving and transmitting channels was discussed. Considering the impact of target depolarization and the demand for along-track interferometry, combined with beam-broaden and high-gain amplifiers, an ISAL system design method that can stably image multiple non-cooperative real targets has been proposed. Under the condition of broadening the transmitting and receiving beams to 3° in the elevation direction for non-cooperative moving vehicles, echo data with a duration of 1 s is obtained. The spatial correlation algorithm combined with along-track interferometry is used to estimate the vibration phase error. The sub-aperture Range-Doppler algorithm is used for imaging. The ISAL imaging results of the moving vehicle validated the high-resolution imaging ability of ISAL and its potential for stable imaging of non-cooperative moving real targets.
Web applications follow a client–server schema, so it is more appropriate for evolutionary test case generation considering both client and server. However, test cases from the client-side are ...composed of event sequences, which are quite time-consuming when executed due to the interaction with the browser. Furthermore, premature convergence is a problem for evolutionary algorithms because of the decline of population diversity. These problems restrict the applicability of evolutionary algorithms in test case generation for web applications.
Parallelization has been proven helpful in optimizing test case generation. So, to improve the efficiency and effectiveness of test generation for web applications, this paper proposes a parallel evolutionary test case generation approach where test cases are generated from the client-side behavior model to cover the sensitive paths of server-side code by using a parallel genetic algorithm based on the island model.
A parallel execution strategy is presented to drive multi-individuals to execute on multi-browsers simultaneously to shorten the execution time of populations during evolution. And an island model with a corresponding migration mechanism and subpopulation evolution strategy is well-designed to increase population diversity during evolution. Meanwhile, the server-side code triggered by parallel individuals is identified to guide the evolution process.
Experiments are conducted on six widely-used web applications, and the results show that compared with the sequential evolutionary test case generation, our approach decreases the iterations and evolution time required by 33.43% and 63.10% on average, respectively. The efficiency of test generation has been greatly enhanced.
This paper provides a parallel evolutionary test case generation for web applications, where the parallel execution strategy is presented to shorten the execution time of populations during evolution, increasing test generation efficiency. Moreover, the island model with a migration mechanism is introduced to increase population diversity during evolution, improving the test generation effectiveness.
•We first propose the parallel evolutionary test case generation for web applications.•A parallel strategy is designed to drive multi-individuals execute simultaneously.•The island model cooperates with genetic algorithm to increase the effectiveness.
Research on the involvement of C1D and its yeast homologues Rrp47 (S. cerevisiae) and Cti1 (S. pombe) in DNA damage repair and RNA processing has remained mutually exclusive, with most studies ...predominantly concentrating on Rrp47. This review will look to reconcile the functions of these proteins in their involvement with the RNA exosome, in the regulation of chromatin architecture, and in the repair of DNA double-strand breaks, focusing on non-homologous end joining and homologous recombination. We propose that C1D is situated in a central position to maintain genomic stability at highly transcribed gene loci by coordinating these processes through the timely recruitment of relevant regulatory factors. In the event that the damage is beyond repair, C1D induces apoptosis in a p53-dependent manner.
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