The Baijiu industry is a significant contributor to both the food industry and the light industry. Its high tax characteristics effectively promote the sustainable development of the regional ...economy. First, the evaluation index system of scientific and technological innovation (STI) and high-quality development of Baijiu industry (HQDBI) were constructed. The entropy-improved CRITIC method was used to measure the weights. Second, the coordination relationship and evolution trend of STI and HQDBI were explored using the coupling coordination model and the Tapio decoupling model. Then, the transfer law and key influencing factors were further investigated using the Markov chain and grey correlation, respectively. The main contribution is the dynamic evolution of the coupling and decoupling relationships from the perspective of multiple Baijiu provinces, and deeply depicts the coordination relationship and evolutionary trends of STI and HQDBI. The results show that: the spatial distribution of the coupling coordination degree shows high values in the east-west and low values in the north-south characteristics. In 2021, a pattern of coordinated development in Baijiu provinces has emerged along the Yangtze River basin. The decoupling state is mainly strong decoupling, but it remains poor in Shanxi. The coordination process is unstable and difficult to achieve leapfrog development. Coordination, sustainability and innovation environment have a greater impact on the coordination of subsystems.
WUSCHEL-related homeobox (WOX) family members play significant roles in plant growth and development, such as in embryo patterning, stem-cell maintenance, and lateral organ formation. The recently ...published cotton genome sequences allow us to perform comprehensive genome-wide analysis and characterization of WOX genes in cotton.
In this study, we identified 21, 20, and 38 WOX genes in Gossypium arboreum (2n = 26, A
), G. raimondii (2n = 26, D
), and G. hirsutum (2n = 4x = 52, (AD)
), respectively. Sequence logos showed that homeobox domains were significantly conserved among the WOX genes in cotton, Arabidopsis, and rice. A total of 168 genes from three typical monocots and six dicots were naturally divided into three clades, which were further classified into nine sub-clades. A good collinearity was observed in the synteny analysis of the orthologs from At and Dt (t represents tetraploid) sub-genomes. Whole genome duplication (WGD) and segmental duplication within At and Dt sub-genomes played significant roles in the expansion of WOX genes, and segmental duplication mainly generated the WUS clade. Copia and Gypsy were the two major types of transposable elements distributed upstream or downstream of WOX genes. Furthermore, through comparison, we found that the exon/intron pattern was highly conserved between Arabidopsis and cotton, and the homeobox domain loci were also conserved between them. In addition, the expression pattern in different tissues indicated that the duplicated genes in cotton might have acquired new functions as a result of sub-functionalization or neo-functionalization. The expression pattern of WOX genes under different stress treatments showed that the different genes were induced by different stresses.
In present work, WOX genes, classified into three clades, were identified in the upland cotton genome. Whole genome and segmental duplication were determined to be the two major impetuses for the expansion of gene numbers during the evolution. Moreover, the expression patterns suggested that the duplicated genes might have experienced a functional divergence. Together, these results shed light on the evolution of the WOX gene family, and would be helpful in future research.
The high-quality development of service industry has become an important engine for promoting sustainable economic development. This paper first constructed the evaluation index system of ...high-quality development of service industry, based on panel data from 2005 to 2020. Second, Kernel density, Markov chain and Dagum Gini coefficient were used to represent the regional differences and dynamic evolution of service industry, and the Koo method was used to explore the characteristics of spatial agglomeration. Finally, social network analysis was used to identify core indicators. The study found that: (1) From 2005 to 2020, the overall level of service industry first decreases and then increases, with Chengdu and Chongqing leading other cities. (2) The development of service industry in the CCEC has large spatial differences, mainly due to inter-regional differences. (3) The level of spatial agglomeration is less variable, with high agglomeration mainly in Chengdu. (4) Indicators such as the level of human capital are the core factors of its high-quality development. This study is of great theoretical and practical significance for the optimization and upgrading of service industry in the CCEC and the synergetic development of the region.
Cotton (Gossypium hirsutum) is the major source of natural textile fibers. Brassinosteroids (BRs) play crucial roles in regulating fiber development. The molecular mechanisms of BRs in regulating ...fiber elongation, however, are poorly understood. pagoda1 (pag1) was identified via an activation tagging genetic screen and characterized by genome walking and brassinolide (BL) supplementation. RNA‐Seq analysis was employed to elucidate the mechanisms of PAG1 in regulating fiber development. pag1 exhibited dwarfism and reduced fiber length due to significant inhibition of cell elongation and expansion. BL treatment rescued its growth and fiber elongation. PAG1 encodes a homolog of Arabidopsis CYP734A1 that inactivates BRs via C‐26 hydroxylation. RNA‐Seq analyses showed that the constitutive expression of PAG1 downregulated the expression of genes involved in very‐long‐chain fatty acids (VLCFA) biosynthesis, ethylene‐mediated signaling, response to cadmium, cell wall development, cytoskeleton organization and cell growth. Our results demonstrate that PAG1 plays crucial roles in regulating fiber development via controlling the level of endogenous bioactive BRs, which may affect ethylene signaling cascade by mediating VLCFA. Therefore, BR may be a critical regulator of fiber elongation, a role which may in turn be linked to effects on VLCFA biosynthesis, ethylene and cadmium signaling, cell wall‐ and cytoskeleton‐related gene expression.
In the present complex electromagnetic environment, radar target detection is threatened by different kinds of interferences, especially mainlobe deceptive interference, which occupies the same ...energy distributions of targets spatially, meaning that targets and interferences cannot be discriminated. To make matters worse, the number of suppressible interferences is limited by the number of physical array elements, leading to the degradation of the suppression performance of traditional radar. In this work, we propose a frequency-increment-based interference suppression method for minimum redundancy frequency diverse array multiple-input multiple-output (MR-FDA-MIMO) radar, which effectively solves the aforementioned two problems. The interference suppression method consists of two steps: (i) in the sidelobe barrage interference suppression stage, the interference-plus-noise covariance matrix is reconstructed to overcome the influence of the true targets and mainlobe deceptive interference on the performance of the beamformer; (ii) in the mainlobe deceptive interference suppression stage, a nonadaptive beamforming method is employed to suppress mainlobe deceptive interference and overcome the impact of insufficient virtual samples on interference suppression performance. Additionally, we design a frequency-increment-based MR-FDA-MIMO radar, fully utilizing the advantages of the virtual array to enhance interference suppression performance and increase the number of interferences. Numerical experiments undertaken demonstrate the effectiveness of the algorithm under different scenarios.
Major urban areas in Henan Province of central China.
data fusion technology is also a key and difficult point in the field of flood research. Remote sensing and text data have different modalities ...and scales, making fusion difficult. This study proposed a remote sensing and text bimodal data fusion model based on UFCLI, and we validated the spatiotemporal distribution of floods and the calculation results of disaster losses. The results show that through the coupling analysis of remote sensing and text bimodal data, rainstorm and flood events can be fully reproduced in space and time. The proposed UFCLI effectively improves the accuracy of remote sensing single-data inversion for urban flood disaster losses. The flood loss in Henan is 121.98 billion yuan, and the accuracy improvement result is R² increased by 0.08 and MAPE decreased by 0.88.
In the case of sudden urban storm flooding with complex spatial and temporal evolution, the traditional hydrological-hydraulic model has many pending parameters, which makes it difficult to accurately calculate large-scale disaster losses. By establishing a theoretical model of bimodal data fusion, we effectively use the complementary spatiotemporal information using remote sensing and text to solve the differences in spatiotemporal scales existing between remote sensing and text data. The timeliness and accuracy of urban flood damage estimation have further improved.
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•A dual-mode data fusion model of remote sensing and text is proposed.•Remote sensing and text are coupled to establish the flood time-space evolution map.•UFCLI model improves the accuracy of urban flood damage calculation.
Range-ambiguous clutter is an inevitable issue for airborne forward-looking array radars, especially with the high pulse repetition frequency (PRF). In this paper, a method to suppress the ...range-ambiguous clutter is proposed in an FDA-MIMO radar with a forward-looking planar array. Compressed sensing FDA technology is used to suppress the range-ambiguous clutter and the forward-looking non-uniformity short-range clutter of radar. Specifically, first, the range ambiguous clutter in different regions is separated by the characteristics of the planar array radar elevation dimension and FDA radar range coupling. Meanwhile, regarding the issue of the FDA radar main lobe moving between coherent pulses, a main lobe correction (MLC) algorithm proposes a solution for the issue, where the FDA radar cannot coherently accumulate signals in the case of non-full angle illumination. Finally, compressed sensing technology and elevation dimension filtering are utilized to suppress the range ambiguous clutter at the receiver, with the approach alleviating the range dependence of clutter in the observation region. A small number of clutter snapshots can obtain an approximately ideal clutter covariance matrix through compressed sensing sparse recovery. The method not only reduces the number of training samples, but also overcomes the problem of clutter non-uniformity in the forward-looking array. Therefore, the clutter suppression problems faced by the high repetition frequency airborne radar forward-looking array structure are solved. At the analysis stage, a comparison among the conventional MIMO and FDA methods is carried on by analyzing the improvement factor (IF) curves. Numerical results verify the effectiveness of the proposed method in range-ambiguous clutter suppression.
Abstract Immunotherapy has emerged as a pivotal modality in cancer treatment, with immune checkpoint inhibitors effectively combating malignancies by impeding crucial pathways within the immune ...system and stimulating patients’ immune responses. Soluble forms of immune checkpoints exhibit a remarkable diversity and can be readily tracked in circulation, holding immense potential as biomarkers for cancer treatment. An increasing number of studies focused on soluble immune checkpoints in cancer have emerged thanks to technological advancements. In this systematic review, we comprehensively summarized the recent studies on soluble immune checkpoints in human cancer risk prediction, outcome prediction, therapeutic applications, and potential molecular mechanisms, which demonstrated the promising future of soluble immune checkpoints in clinical applications. The clinical relevance of soluble immune checkpoints has been recognized in multiple cancers, yet the therapeutic applications and mechanisms remain obscure. Interpreting the impacts and mechanisms of soluble immune checkpoints could shed a light on the novel strategies of cancer screening, treatments, and outcome prediction.
Cynanchum komarovii Al Iljinski is a desert plant that has been used as analgesic, anthelminthic and antidiarrheal, but also as a herbal medicine to treat cholecystitis in people. We have found that ...the protein extractions from C. komarovii seeds have strong antifungal activity. There is strong interest to develop protein medication and antifungal pesticides from C. komarovii for pharmacological or other uses.
An antifungal protein with sequence homology to thaumatin-like proteins (TLPs) was isolated from C. komarovii seeds and named CkTLP. The three-dimensional structure prediction of CkTLP indicated the protein has an acid cleft and a hydrophobic patch. The protein showed antifungal activity against fungal growth of Verticillium dahliae, Fusarium oxysporum, Rhizoctonia solani, Botrytis cinerea and Valsa mali. The full-length cDNA was cloned by RT-PCR and RACE-PCR according to the partial protein sequences obtained by nanoESI-MS/MS. The real-time PCR showed the transcription level of CkTLP had a significant increase under the stress of abscisic acid (ABA), salicylic acid (SA), methyl jasmonate (MeJA), NaCl and drought, which indicates that CkTLP may play an important role in response to abiotic stresses. Histochemical staining showed GUS activity in almost the whole plant, especially in cotyledons, trichomes and vascular tissues of primary root and inflorescences. The CkTLP protein was located in the extracellular space/cell wall by CkTLP::GFP fusion protein in transgenic Arabidopsis. Furthermore, over-expression of CkTLP significantly enhanced the resistance of Arabidopsis against V. dahliae.
The results suggest that the CkTLP is a good candidate protein or gene for contributing to the development of disease-resistant crops.
To get a broader view on the molecular mechanisms underlying somatic embryogenesis （SE） in cotton （Gossypium hirsutum L.）, global analysis of cotton transcriptome dynamics during SE in different ...sister lines was performed using RNA-Seq. A total of 204 349 unigenes were detected by de novo assembly of the 214 977 462 Illumina reads. The quantitative reverse transcription-polymerase chain reaction （qRT-PCR） measurements were positively correlated with the RNA-Seq results for almost all the tested genes （R2 -- 0.841, correlation was significant at the 0.01 level）. Different phytohormone （auxin and cytokinin） concentration ratios in medium and the endogenous content changes of these two phytohormones at two stages in different sister lines suggested the roles of auxin and cytokinin during cotton SE. On the basis of global gene regulation of phytohormone-related genes, numerous genes from all the differentially expressed transcripts were involved in auxin and cytokinin biosynthesis and signal transduction pathways. Analyses of differentially expressed genes that were involved in these pathways revealed the substantial changes in gene type and abundance between two sister lines. Isolation, cloning and silencing/ overexpressing the genes that revealed remarkable up- or down-expression during cotton SE were important. Furthermore, auxin and cytokinin play a primary role in SE, but potential cross-talk with each other or other factors remains unclear.
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