In this study, we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the template-synthesized sol−gel nanotubes for chemical separation of estrone. ...The silica nanotubes were synthesized within the pores of nanopore alumina template membranes using a sol−gel method by simultaneous hydrolysis of a silica monomer−imprinted molecule complex and tetraethoxysilane (TEOS). A covalent imprinting strategy was employed by generating a sacrificial spacer through the reaction of the isocyanate group of 3-(triethoxysilyl)propyl isocyanate and a phenol moiety of estrone to form a thermally cleavable urethane bond. This allowed us to remove the imprinted estrone by simple thermal reaction and to simultaneously introduce functional groups into the cavity formed by the silica nanotubes. Experiments indicated that estrone could be bound selectively by such an approach and have a binding affinity of 864 ± 137 (n = 3).
Fat overload syndrome is a rare and severe adverse reaction triggered by the infusion of a single source of lipid emulsion, resulting in elevated blood triacylglycerol (TG) levels. The majority of ...literature reports focus on cases of fat overload syndrome in patients with mild symptoms. This case is significant because it demonstrates the diagnostic and therapeutic experience and provide valuable insights for the management for severe fat overload syndrome.
We present a case report of a female patient who developed fat overload syndrome following prolonged and excessive infusion of lipid emulsion after colon resection surgery. In the setting of compromised immune function and malnutrition, the patient's pulmonary infection and respiratory distress symptoms have further exacerbated. Hence, in addition to severe pancreatitis, the patient has also contracted severe pneumonia. Upon admission, tracheal intubation, plasma exchange and blood perfusion were performed. Subsequently, comprehensive treatment was provided, including anti-infection, antispasmodic, acid suppression, enzyme inhibition, as well as targeted supportive measures to stabilize electrolytes and nutritional status. After treatment, there was a progressive reduction in blood lipid levels. After assessing the relevant risks, it was deemed necessary to perform an emergency computed tomography (CT)-guided percutaneous drainage tube placement procedure targeting the necrotic area of the pancreas while the patient was still intubated. Finally, the patient was discharged from the hospital.
The case highlights the association between fat overload syndrome and pancreatitis as well as the use of lipid emulsions and suggests the treatment strategies for severe fat overload syndrome.
Single-crystalline cryptomelane-type manganese oxide octahedral molecular sieve (OMS-2) nanoneedles and nanorods were prepared by a solution-phase approach in the presence of an ionic liquid ...1-n-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4). BMIMBF4 can act as a cosolvent, structure-directing agent, and reducing reagent in the reaction system. Based on the redox reaction of MnCl2 and KMnO4 in the mixed solvents of water and BMIMBF4, the formation of OMS-2 nanoneedles followed the rolling mechanism with lamellae as an intermediate. However, the direct reaction of KMnO4 with BMIMBF4 resulted in the formation of OMS-2 nanorods with diameters as small as 3−6 nm. The formation mechanism of OMS-2 nanostructures was discussed.
The tumor-suppressive functions of interferon regulatory factor 6 (IRF6) in some tumors have been preliminarily established, but its pathogenesis and underlying molecular mechanisms in breast cancer, ...the most common malignancy in women, remains poorly understood.
Pairs of typical breast cancer cell lines (high- and low-aggressive) in addition to 27 breast cancer tissue samples and 31 non-cancerous breast tissues were used to investigate the expression level of IRF6 and Lentivirus-mediated gain-of-function studies, short hairpin RNA-mediated loss-of-function studies in vivo and in vitro were used to validate the role of IRF6 in breast cancer. Next, we performed RNA-Seq analysis to identify the molecular mechanisms of IRF6 involved in breast cancer progression.
Our findings showed that IRF6 was downregulated in highly invasive breast cancer cell lines but upregulated in poorly aggressive ones. Functional assays revealed that elevated IRF6 expression could suppress cell proliferation and tumorigenicity, and enhanced cellular chemotherapeutic sensitivity. To identify the molecular mechanisms involved, we performed a genome-wide and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis in breast cancer cells using RNA sequencing of gene expression profiles following the overexpression of IRF6. Genome-wide and KEGG analyses showed that IRF6 might mediate the PI3K-regulatory subunit PIK3R2, which in turn modulated the PI3K/AKT pathway to control breast cancer pathogenesis.
We provide the first evidence of the involvement of IRF6 in breast cancer pathogenesis, which was found to modulate the PI3K/AKT pathway via mediating PIK3R2; indicating that IRF6 can be targeted as a potential therapeutic treatment of breast cancer.
•Co3O4/C nanosheet array on nickel foam was synthesized by hydrothermal method, followed by magnetron sputtering of a carbon layer.•The Co3O4/C nanosheet array electrode delivers an initial discharge ...capacity of 1340.4mAhg−1 at 0.1C.•After 100 cycles, the capacity retention of the Co3O4/C is 88.8% of its initial discharge capacity.•The enhanced electrochemical performances are attributed to the porous nanosheet array and the thin carbon layer coated on Co3O4 nanosheets.
Freestanding Co3O4/C nanosheet array growing directly on nickel foam substrate was synthesized using a hydrothermal approach, followed by a direct current magnetron sputtering of a thin carbon layer. The Co3O4 nanosheets with thicknesses of 10–20nm are interconnected with each other, forming a highly open net-structure. The Co3O4/C nanosheet array electrode delivers an initial discharge capacity of 1340.4mAhg−1 at 0.1C, and maintains a high specific capacity of 912.1mAhg−1 at room temperature and can still remain 88.8% of its initial discharge capacity at 55°C at 1C after 100 cycles. The enhanced electrochemical performances are attributed to the porous nanosheet array and the thin carbon layer coated on Co3O4 nanosheets, which cannot only facilitate Li+ and electron transportation in the electrode, but also improve its structure stability during cycling.
Chiral β-amino alcohols were afforded by desymmetric ring-opening of
meso-epoxides with anilines catalyzed by chiral Yb triflate complex in up to 80.1% ee.
Hydroxyapatite (HAP) hierarchical microspheres were synthesized by a facile hydrothermal method using calcium nitrate and ammonium dihydrogen phosphate through controlling complexing agents. The ...influences of two kinds of complexing agents (potassium sodium tartrate tetrahydrate and trisodium citrate) and reaction time on the morphology of HAP crystals have been investigated. These results indicate that complexing agents have a great influence on the morphology of HAP. When potassium sodium tartrate tetrahydrate was used as complexing agent, HAP flowers were composed of the network of nanosheet building blocks. Well-crystallized HAP dandelions with nanorods radiating from the center can be obtained by the introduction of trisodium citrate. Broader XRD diffraction peaks imply a nanometer scale size. Based on XRD and SEM results, the formation mechanism of HAP crystals has been discussed. The hierarchically structured HAP microspheres were explored as drug carriers. The results indicate that HAP flowers and dandelions showed a favorable sustained release property for ibuprofen; thus, they are very promising for application in drug delivery.
To analyze of the transmission characteristics of a cluster of COVID-19 cases in Chongqing and evaluate the infectivity of COVID-19 in the incubation period.
A retrospective survey was conducted by ...using unified questionnaire through field and telephone interviews among 129 close contacts of COVID-19 cases. The relationship of transmission was indicated by transmission chain, and the infectivity was analyzed by the contact history.
This cluster of COVID-19 cases occurred after a classmate party involving members in three families and work fellows in a factory (
(0)=3.8). The infection rate during the incubation period was 17.57%. On average, it was infectious three days before onset. There was significant difference in infection rate among different contact modes (
(2)=15.10,
<0.01), There was significant difference in infection rate among single exposureswith different time length (
(2)=25.08,
<0.01).
COVID-19 is highly infectious in the incubation period. The more confined the space is, the higher the risk
Germline mutations in the tumor suppressor gene BRCA1 predispose women to breast cancer, however somatic mutations in the gene are rarely detected in sporadic cancers. To understand this phenomenon, ...we examined mouse models carrying conditional disruption of Brca1 in mammary epithelium in either p53 wild type (wt) or heterozygous backgrounds. Although a p53(+/-) mutation significantly accelerated tumorigenesis, both strains developed mammary tumors in a stochastic fashion, suggesting that multiple factors, in addition to p53 mutations, may be involved in Brca1 related tumorigenesis. A unique feature of Brca1 mammary tumors is their highly diverse histopathology accompanied by severe chromosome abnormalities. The tumors also display extensive genetic/molecular alterations, including overexpression of ErbB2, c-Myc, p27 and Cyclin D1 in the majority of tumors, while they were virtually ERalpha and p16 negative. Translocations involving p53 were also identified which lead to abnormal RNA and protein products. In addition, we generated cell lines from mammary tumors and found that the cells retained many of the genetic changes found in the primary tumors, suggesting that these genes may be players in Brca1-associated tumorigenesis. Despite their distinct morphology, all cultured tumor cells were Tamoxifen resistant but highly sensitive to Doxorubicin or gamma-irradiation, suggesting that these methods would be effective in treatment of this disease.
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