Digital video is widely used to record people’s daily lives and share people’s moods, but few researchers have conducted research on the consistency of emotional expression between short videos and ...music. In order to be able to match the appropriate background music to the short video image autonomously and efficiently, the paper analyzed the emotional connection between the two from the audio-visual synesthesia. First, emotional semantics was used as a bridge to connect video data and music data, and a video-music synesthesia data set based on semantic words was constructed. Then, an attention mechanism was incorporated to better extract key features in video images. In the extraction of music features, an improved lenet5 network was used, and the optimal network parameters were determined through experiments. Finally, the two types of features were fused and the mutual retrieval between video and music was performed. In order to compare the performance of different models, different CNN models were calculated in the processing of video images, including VGG16, VGG19, AlexNet and GoogleNet, and the attention mechanism was added to each network for calculation to compare its retrieval accuracy. In the processing of music data, different CNN algorithms were also used for comparative experiments, and networks with different layers were used to determine the optimal results. The experimental results show that the audiovisual synesthesia retrieval model based on emotion can effectively measure the emotional similarity between video images and music, and the method of the paper can produce a good match between them. The research method of the paper is the exploration of computer synesthetic intelligence, which can stimulate the creative inspiration of image and music creative designers. While enhancing the emotional experience of digital products, it also improves the efficiency and quality of development.
Low-temperature tenderization is an important aspect in the processing of sea cucumber. Oxidative reactions induce protein aggregation or degradation during thermal treatment. However, whether ...protein oxidation occurs in sea cucumber during tenderization is unclear. Moreover, the effects of heat treatment on the protein structure in sea cucumber have not been well explored. In this study, Apostichopus japonicus body wall (AJBW) were tenderized at 37 °C and changes in the protein microstructure of AJBW and the relative indicators of oxidative reactions, including carbonyl, sulfhydryl, and amino constituents, were investigated. The findings indicated that the collagen fibers were depolymerized and the maximum denaturation temperature of collagen was significantly reduced after tenderization, suggesting that this process induced structural changes in collagen fibers. Furthermore, amino acid side-chain modifications and the formation of an unstable secondary and tertiary conformation indicated oxidation of the water-soluble proteins. The findings revealed that tenderization of AJBW caused the destruction of the collagen fiber structure and resulted in protein oxidation and degradation. Therefore, our study lays the foundation for subsequent research for determining the mechanism of tenderization.
•Heating Apostichopus japonicus at 37 °C resulted in tenderization over time.•Collagen fibers were depolymerized and thermostability reduced after tenderization.•Carbonyl content increased and sulfhydryl content decreased after tenderization.•Unstable secondary and tertiary conformation changes were found after tenderization.
Poultry is one of the most widely consumed animal-based foods in the world. Understanding the characteristics of bacterial communities present during processing and the targeted design of natural ...preservatives could improve the quality and safety concerns of poultry industry. In this study, pathogens and spoilage bacteria arising from the processing of poultry products were identified using high-throughput 16S rRNA sequencing and traditional culture screening. Ten dominant pathogenic and spoilage bacteria were isolated, including 8 g-positive and 2 g-negative bacteria. The mechanism and antibacterial activity of Kaempferia galanga Linn. extract (KGE), a spice added to the poultry products, against the targeted pathogenic bacteria were analyzed further. KGE exhibited significant antibacterial activity against all tested pathogenic bacteria. The antibacterial mechanism of KGE involved alteration of cell integrity and cell membrane permeability, resulting in an outflow of intracellular substances, such as nucleic acids, proteins, and alkaline phosphatase. Scanning electron microscopy further confirmed KGE-mediated cell membrane damage and cytoplasmic leakage. The chemical characterization indicated that the large amounts of acetic acid present in KGE might account for its excellent antibacterial activity. Taken together, the study confirms the excellent antibacterial activity of KGE and its potential use as a natural preservative for poultry products.
•Identification of pathogens and spoilage bacteria arising from the processing of poultry products.•An antibacterial substance extracted from Kaempferia galangal L., namely KGE.•KGE inhibited the growth of dominant pathogens and spoilage bacteria in poultry products.•The antibacterial mechanism of KGE involved alteration of cell integrity and cell membrane permeability.•The large amount of acetic acid in KGE might be a key determinant of its antibacterial activity.
An ultrasensitive signal amplification strategy has been developed using Au@Cu-MOF nanocapsules as an electrochemical platform for the determination of glutathione (GSH) based on the signal output ...from solid-state electrochemistry of cuprous chloride (CuCl). The Au@Cu-MOF nanocapsule, with a metal–organic framework (MOF) sandwich shell and an encapsulated Au nanoparticle layer, was prepared via coordinate replication of a Cu2O redox-template strategy. Also, the Au@Cu-MOF nanocapsule-modified electrode achieved boosting signal output in the presence of chlorine ions (Cl–) by solid-state electrochemistry of CuCl, which showed a pair of remarkable peaks. However, in the presence of GSH, the phenomenon termed as “crowding-out effect” appeared, in which the specific Cu–GSH interaction triggered the competitive reaction with the conversion of CuCl into nonelectroactive substance of Cu–GSH, leading to a sharp decrease in the peak current of CuCl. The Au@Cu-MOF nanocapsule-modified electrode showed high sensitivity toward GSH owing to the specific electrochemical output at a relatively low potential, which can effectively shield the possible interferences from complex physiological surroundings. The crowding-out effect can also improve the selectivity, particularly for complex analytical samples in terms of electrode fouling. The electrochemical sensor showed a wide linear range of 0.01–40 nM and 40 nM to 10 μM, with a detection limit of 2.5 pM (S/N = 3). The sensor can be utilized for the determination of GSH in various real vegetable and serum samples with satisfactory results. Besides, it can also be applied for probing GSH in the lysates of Romas cells with good sensitivity, which brings dawn for early diagnosis of cancer in clinics. Moreover, the ultrasensitive and selective solid-state CuCl electrochemistry-promoted signal amplification strategy as well as the excellent stability and special nature of Au@Cu-MOF nanocapsules may promise their wide application in biological and food analysis fields.
•A nanocrystalline Cu-NbC nanocomposite was fabricated.•The nanocomposite exhibits a combination of high strength and high electrical conductivity.•The nanocomposite is highly thermal stable.
A ...combination of high tensile strength of 900 MPa and a good electrical conductivity of 50% International Annealed Copper Standard (IACS) was achieved in a nanocrystalline Cu-0.1 wt%Al (grain size: 96 nm) matrix composite reinforced with 6.4 vol% NbC nanoparticles (6.5 nm). The composite was produced by combining high energy mechanical milling (HEMM), sintering and powder compact extrusion. The composite was highly thermally stable that a higher electrical conductivity of 57% IACS was achieved, while maintaining a high tensile strength of 722 MPa and good ductility after annealing at 1000 °C for 5 h.
The Front Cover shows that a medium‐chain alcohol dehydrogenase modified in the loop is efficiently catalyzing N‐Boc‐3‐pyrrolidone to produce (S)‐N‐Boc‐3‐pyrrolidinol. In their Research Article, H. ...Wang and co‐workers proposed that loop‐loop interaction region acts like the “bridges” between loops and could be used to adjust conformational dynamics of the enzyme, thus modifying its catalytic efficiency. Combined with the analysis of residue orientation and interaction network, three loop‐loop interaction regions were selected for mutagenesis. After screening, a mutant with 94.5‐fold enhancement in catalytic efficiency was obtained. The results show that the residues located in the loop‐loop interaction region could be used to effectively alter the loop conformation, thereby affecting the catalytic properties of the enzyme. More information can be found in the Research Article by H. Wang and co‐workers.
The objective was to devise a nomogram integrating radiomics features, sonographic characteristics, and immunologic indices to distinguish between IBC and IBC-IC.
The preoperative ultrasonography ...(US) findings and pathological data from 244 BCE patients (115 IBC-IC and 129 IBC) diagnosed with IDC through surgical confirmation were retrospectively gathered. The study utilized the BI-RADS lexicon from the ACR to interpret the findings. 1125 radiomics features were derived from the regions of breast lesions, coupled with pathological data, to establish a predictive model employing the LASSO technique. The model's performance was examined by the AUC, along with sensitivity, and specificity measures, to evaluate its effectiveness in differentiating between the two groups of subjects.
Four prognostic models for IDC-IC were developed, comprising a conventional ultrasound (CUS) signature, pathology signature nomogram, radiomics signature, and combined signature. The combined model exhibited the highest diagnostic efficacy, achieving the AUC of 0.893, sensitivity of 0.875, and specificity of 0.835 in the training set, and 0.835, 0.800, and 0.789 in the testing set.
The combined model, integrating CUS, pathology information, and radiomics features derived from CUS, exhibited a notable capacity for preoperatively distinguish IDC-IC. This capability holds promise for guiding personalized surgical strategies for BC individuals undergoing breast-conserving surgery (BCS).
We explored the effects of compound 33, a synthetic chalcone derivative with antioxidant activity, on lipopolysaccharide (LPS)-induced acute lung injury (ALI). Compound 33, dexamethasone or vehicle ...was administered intragastrically to mice 6 h before intratracheal instillation of LPS. After 24 h, the effects of compound 33 on alveolar structural damage were evaluated by assessing lung morphology and the wet/dry weight ratio. Protein and proinflammatory cytokine levels and superoxide dismutase activity were also examined in the cell free supernatant of bronchoalveolar lavage fluid. Additionally, we investigated the anti-inflammatory and antioxidant activity of compound 33
and its effects on the MAPK/NF-κB and Nrf2/HO-1 pathways. Pretreatment with compound 33 prevented LPS-induced structural damage, tissue edema, protein exudation, and overproduction of proinflammatory mediators. The effects of compound 33 were similar to or greater in magnitude than those of the positive control, dexamethasone. Moreover, compound 33 exerted anti-inflammatory and antioxidant effects
by inhibiting the MAPK/NF-κB pathway and activating the Nrf2/HO-1 pathway. Compound 33 may therefore be a promising candidate treatment for ALI.
In current study, a self-nanoemulsifying drug delivery system (SNEDDS) of persimmon (
Diospyros kaki) leaf extract (PLE) was developed and characterized to compare its
in vitro dissolution and ...relative bioavailability with commercially available tablets (Naoxinqing tablets). Pseudo-ternary phase diagrams were constructed by phase diagram by micro plate dilution (PDMPD) method, of which the evaluation method was improved to use Multiskan Ascent for identifying turbidity. The formulation of PLE-loaded SNEDDS was optimized by an extreme vertices experimental design. The optimized nanoemulsion formulation, loading with 44.48
mg/g PLE total flavonoids, consisted of Cremophor EL, Transcutol P, Labrafil M 1944 CS (56:34:10, w/w), and it remained stable after storing at 40
°C, 25
°C, 4
°C for at least 6 months. When diluted with water, the SNEDDS droplet size was 34.85
nm and the zeta potential was −6.18
mV. Compared with the commercial tablets, the AUC of both quercetin and kaempferol, which are representative active flavonoids of PLE, was increased by 1.5-fold and 1.6-fold respectively following oral administration of PLE-loaded SNEDDS in fasting beagle dogs. These results indicate that SNEDDS is a promising drug delivery system for increasing the oral bioavailability of PLE.
Loops typically allosterically communicate with active sites, and their conformational dynamics can affect the catalytic properties of enzymes. Herein, by manipulating the loop conformational ...dynamics via adjusting loop‐loop interactions, a medium‐chain alcohol dehydrogenase (Syn94) was engineered to enhance the activity without enantioselectivity reduction. Syn94, from Synechocystis sp. PCC 6803 was identified, and it demonstrated good enantioselectivity (>99.9 % ee) for synthesizing (S)‐N−Boc‐3−pyrrolidinol ((S)‐Boc−PL), a key intermediate of darifenacin, but with low catalytic efficiency. To enhance its activity, three regions for loop‐loop interactions near the active pocket were identified for mutagenesis. After the iteration of two effective sites, the best mutant, S89T/P282R, with a 94.5‐fold enhancement in catalytic efficiency, was successfully obtained. By establishing a co‐expression system of S89T/P282R and glucose dehydrogenase (GDH), up to 1.0 M (180.2 g/L) substrate could be completely reduced to (S)‐Boc−PL within 7 h, with 617 g/L/d space‐time‐yield. Molecular dynamics simulations revealed that the enhanced activity was related to the stabilization of two loops, D41‐V58 and W87‐T112.
Engineered Enzyme Catalysis: Residues at loop‐loop interaction regions were successfully used to induce conformational dynamic changes, and a medium‐chain ADH was engineered to exhibit higher activity based on the strategy. The established process required only low catalyst and cofactor loadings, and provided a practical solution to the efficient biosynthesis of the alcohol (S)‐N−Boc‐3−pyrrolidinol, with >99 % conversion and >99.9 % ee.
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