Prior research suggests that critical participatory action research (CPAR) - a research approach that centers democratic participation, agency, and collective capacity-building - may be one way to ...improve teachers' professional development and increase their feelings of agency and well-being. Engaging in CPAR has the potential to foster teachers' positive relationships, build their critical consciousness, and increase their feelings of civic empowerment. The current project explores teachers' experiences in a yearlong CPAR professional development course, investigating whether this professional development course may support teacher's self-reported sense of agency and their self-reported sense of well-being. Drawing on interview data, the findings suggest that teachers felt an increased sense of agency and well-being while engaged in the CPAR workshop. Although some teachers were able to transfer these feelings to their school contexts, the levels and forms of transferability seemed related to specific contextual factors. Thus, this study indicates that the benefits of CPAR may not always transfer across settings.
With the development of Earth observation technology, hyperspectral image (HSI) and light detection and ranging (LiDAR) data collaborative monitoring has shown great potential in the ecological ...protection and restoration of coastal wetlands. However, due to the different working principles adopted by the HSI sensor and the LiDAR sensor, the data obtained by them have different distribution characteristics. The distribution difference limits the fusion of HSI and LiDAR data, bringing a great challenge for coastal wetland classification. To tackle this problem, a multisource feature embedding and interaction fusion network (MsFE-IFN) is proposed for coastal wetland classification. First, the HSI and LiDAR data are embedded in the same feature space, where the feature distribution of multisource remote sensing is aligned to alleviate data distribution differences. Second, the aligned HSI and LiDAR features interact information in channels and pixels, which is able to establish the relationship of spectral, elevation, and geospatial. Third, the HSI and LiDAR features are sent into the feature fusion network, in which the low-frequency residual is retained to enrich intraclass features. Finally, the fused feature is applied for final class prediction. Experiments conducted on three coastal wetland HSI-LiDAR datasets created by ourselves demonstrate the superiority of the proposed MsFE-IFN for coastal wetland classification. The codes will be available from the website: https://github.com/bigshot-g/IEEE_TGRS_MsFE-IFN .
As an environmentally friendly and efficient method, successive two-step fermentation has been applied for extracting chitin from shrimp shells. To screen out the microorganisms for fermentation, a ...protease-producing strain,
Exiguobacterium profundum
, and a lactic acid-producing strain,
Lactobacillus acidophilus
, were isolated from the traditional fermented shrimp paste. Chitin was extracted by successive two-step fermentation with these two strains, and 85.9 ± 1.2% of protein and 95 ± 3% of minerals were removed. The recovery and yield of chitin were 47.82 and 16.32%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM) were used to characterize the chitin. The crystallinity index was 54.37%, and the degree of deacetylation was 3.67%, which was lower than that of chitin extracted by the chemical method. These results indicated that successive two-step fermentation using these two bacterial strains could be applied to extract chitin. This work provides a suitable strategy for developing an effective method to extract chitin by microbial fermentation.
Chitin extraction from shrimp waste by protease-producing microorganisms was a positive and simple method. To improve the protease activity of microorganism used for the extraction of chitin, ...atmospheric and room temperature plasma technology was adopted to induce mutations in Exiguobacterium profundum, a protease-producing bacterium, which was isolated from traditional fermented shrimp paste. After several rounds of screening, the mutant numbered 10017 was screened. The hereditary properties of the mutant were found to be stable after a series of passages. This strain was subsequently used in the deproteinization process, which could remove 91.48% ± 2.60% protein, and the chitin recovery was 70.18 ± 2.68%. Fourier transform infrared spectrometry, X-ray diffraction, and scanning electron microscopy was adopted to compare the characteristics of the chitin extracted from mutagenized and wild-type strain fermentation. The crystallinity indices were 80.72% and 82.46%, and the degrees of deacetylation were 15.78% and 27.84%. These results indicated that the deproteinization by mutagenized strain fermentation might be applied to the production of chitin. Thus, the present study provides an appropriate strategy to develop an efficient method to improve protease activity in microbial fermentation.
•Exiguobacterium profundum was isolated from traditional fermented shrimp paste.•Exiguobacterium profundum was subjected to ARTP mutation.•Chitin from shrimp waste was extracted by mutagenized strain fermentation.•The obtained sample was compared with wild-type strain using FT-IR, XRD and SEM.
•Children with ASD vary in their engagement during play.•Parents are responsive to their children’s musical and nonmusical play.•During musical play parents use more physical play and fewer verbal ...responses.•Results have implications for use of music in parent mediated therapy.
Parent-child play interactions offer an important avenue for supporting social development in children with autism spectrum disorder (ASD). Musical play is a natural and ubiquitous form of parent-child play. As a familiar, reinforcing, and predictable activity, musical play may support parent-child interactions by scaffolding children’s attention to the play activities, while also providing parents with a familiar and accessible context to promote parental responsiveness. However, musical play may also impede interactions due to its sensory and repetitive components.
12 parent-child dyads of preschoolers with ASD were video-recorded during a ten-minute play session that included musical and non-musical toys. Interactions were coded for parent and child musical engagement, as well as parental responsiveness.
Parent-child dyads varied in their amount of musical engagement during play, which was not related to children’s language level. Overall, parents showed similar levels of responsiveness to children’s play across musical and non-musical activities, but type of parental responsiveness differed depending on the play context. Parents provided significantly more physical play responses and significantly fewer verbal responses during musical vs. non-musical engagement with their child.
There are substantial individual differences in children with ASD’s musical engagement during a parent-child free play. Children’s musical engagement impacted type of parental responsiveness, which may relate to the familiarity, accessibility, and sensory nature of musical play/toys. Results suggest that musical play/toys can both support and hinder different types of parental responsiveness with implications for incorporation of musical activities into interventions.
Asian American (AsA) youth comprise a large and fast-growing proportion of the U. S. population. AsA youth have comparable and sometimes higher rates of mental health concerns compared to White ...youth, but are significantly less likely to access mental health services. Cognitive-behavioral therapy (CBT) was originally designed by and for White and Western populations, but might provide clinical benefits for AsA youth because several CBT characteristics overlap with some AsA values (e.g., directive therapeutic style; family involvement). Despite this promise, there has yet to be a synthesis of evidence on the effectiveness of CBT, either culturally-adapted or non-adapted, for AsA youth. A systematic narrative review identified randomized controlled trials (RCTs) of CBT conducted with AsA youth. Electronic databases used included PsycINFO, Web of Science, PubMed, and ProQuest. Our search yielded 2,059 articles, of which, 8 RCTs (386 participants) met inclusion criteria. Studies were heterogeneous across targeted problems (e.g., phobia, depression) and age (M = 8.4-22.1 years). Findings suggest that both culturally-adapted and non-adapted CBT (3 and 5 RCTs, respectively) were effective in reducing a range of emotional and behavioral problems for AsA youth. The dearth of studies with AsA youth underscores the need for enhancing the cultural responsiveness of clinical research and practice, as well as harnessing community-engaged methods to improve the accessibility and uptake of evidence-based mental health services for AsA youth.
This paper introduces TAM-SenticNet, a Neuro-Symbolic AI framework uniquely designed for early depression detection through social media content analysis. Merging neural networks for feature ...extraction and sentiment analysis with advanced symbolic reasoning, TAM-SenticNet overcomes the limitations of traditional diagnostic tools, particularly in real-time responsiveness and interpretability. The symbolic reasoning, powered by SenticNet, provides a deep, structured understanding of emotional expressions, greatly enhancing model explainability and logical inference. Empirical evaluations reveal that TAM-SenticNet excels beyond existing models in performance metrics, achieving a Precision of 0.665, Recall of 0.881, and F1-score of 0.758, coupled with superior latency metrics, including ERDE5 and ERDE50 at 0.025, LatencyTP at 1.0, and Flatency at 0.675. These achievements highlight TAM-SenticNet’s cutting-edge approach to early depression detection, making it a pioneering tool in the application of AI for mental health informatics.
Due to the environmental persistence and various health problems associated with per- and polyfluoroalkyl substances (PFASs), they have come under increased public scrutiny. However, the efficient ...extraction of PFASs from complex media remains challenging. Herein, an olefin-linked covalent organic framework (COF-CN) has been prepared via a Knoevenagel condensation reaction, followed by reduction using LiAlH4 to form an amine-functionalized COF (COF-NH2). The characterization results demonstrated that the crystal structure was maintained during the post-modification step. Isothermal and kinetic adsorption studies showed the higher affinity of COF-NH2 toward PFASs. Based on density functional theory, the adsorption mechanism of the stable six-member-ring structure formed between COF-NH2 and PFASs via hydrogen bonding was tentatively revealed. After optimizing the solid-phase microextraction parameters, legacy and emerging PFASs were efficiently extracted from fish using the COF-NH2 coating, followed by detection using ultra-performance liquid chromatography-tandem mass spectrometry. The method exhibited ideal linearity, low limits of quantification, excellent precision, and high relative recoveries. Finally, the bioconcentration kinetics for goldfish was studied, which can provide a feasible platform for investigating the accumulate ion and toxicity of PFASs.
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•Olefin-linked organic framework owning NH2 group (COF-NH2) is prepared.•COF-NH2 is utilized for the solid-phase microextraction of PFASs from fish.•Density functional theory is used to discuss the adsorptive mechanism.
Objectives
YTHDF1 is known as a m6A reader protein, and many researches of YTHDF1 focused on the regulation of mRNA translation efficiency. However, YTHDF1 is also related to RNA degradation, but how ...YTHDF1 regulates mRNA degradation is indefinite. Liquid‐liquid phase separation (LLPS) underlies the formation of membraneless compartments in mammal cells, and there are few reports focused on the correlation of RNA degradation with LLPS. In this research, we focused on the mechanism of YTHDF1 degraded mRNA through LLPS.
Materials and Methods
The CRISPR/Cas9 knock out system was used to establish the YTHDF1 knock out (YTHDF1‐KO) cell lines (HEK293 and HeLa) and METTL14 knock out (METTL14‐KO) cell line (HEK293). 4SU‐TT‐seq was used to check the half‐life changes of mRNAs. Actinomycin D and qPCR were used to test the half‐life changes of individual mRNA. RNA was stained with SYTO RNA‐select dye in wild type (WT) and YTHDF1‐KO HeLa cell lines. Co‐localization of YTHDF1 and AGO2 was identified by immunofluorescence. The interaction domain of YTHDF1 and AGO2 was identified by western blot. Phase separation of YTHDF1 was performed in vitro and in vivo. Fluorescence recovery after photobleaching (FRAP) was performed on droplets as an assessment of their liquidity.
Results
In this research, we found that deletion of YTHDF1 led to massive RNA patches deposited in cytoplasm. The results of 4SU‐TT‐seq showed that deletion of YTHDF1 would prolong the half‐life of mRNAs. Immunofluorescence data showed that YTHDF1 and AGO2 could co‐localize in P‐body, and Co‐IP results showed that YTHDF1 could interact with AGO2 through YT521‐B homology (YTH) domain. We confirmed that YTHDF1 could undergo phase separation in vitro and in vivo, and compared with AGO2, YTHDF1 was more important in P‐body formation. The FRAP results showed that liquid AGO2 droplets would convert to gel/solid when YTHDF1 was deleted. As AGO2 plays important roles in miRISCs, we also found that miRNA‐mediate mRNA degradation is related to YTHDF1.
Conclusions
YTHDF1 recruits AGO2 through the YTH domain. YTHDF1 degrades targeting mRNAs by promoting P‐body formation through LLPS. The deletion of YTHDF1 causes the P‐body to change from liquid droplets to gel/solid droplets, and form AGO2/RNA patches, resulting in a degradation delay of mRNAs. These findings reveal a previously unrecognized crosstalk between YTHDF1 and AGO2, raising a new sight of mRNA post‐transcriptional regulation by YTHDF1.
Working model: YTHDF1 interacts with AGO2 (AGO2 perhaps along with miRNA) through YTH domain, then undergoes LLPS to aggregate more components of miRISCs, and leading to P‐body formation for mRNA degradation. Deficiency of YTHDF1 disrupts the interaction between YTHDF1 and AGO2, leads to the conversion of AGO2 liquid droplets to gels/solids and substantially increases the mRNA stability.
Followed by the high quality of management in investigators’ training, trial development, human resources, and research funding, SGOG has initiated phase III clinical trials to improve the future ...clinical practice. To confirm or compare a novel or unconfirmed traditional treatments with or without updated standard treatment approaches, irrelevant to new drugs/devices, apart from the academic center/person owning an intellectual property right To confirm or compare approved new drugs/devices with or without standard treatment approaches To compare unapproved drugs/devices with standard treatment approaches Protocol Designed and developed by the leading study group, then reviewed and approved by the trial steering committee Designed and developed by the leading study group and the industry partner, then reviewed and approved by the trial steering committee Designed and developed by the industry for the approval of new drugs/devices Independent DMC Organized by the academic center Organized by the academic center Organized by the industry Founding Government /The academic center Government /The academic center and industry co-funding The industry Patients’ benefit Compared to Type C, benefit greatly or comparably Compared to Type C, benefit greatly or comparably, but still face the risk of drug/device failure and potential adverse events Benefit greatly or failure, and potential adverse events Database property The academic center CRO contracted by the academic center CRO contracted by the industry Intellectual property The academic center Partially belong to the academic investigators The industry CRO: Each serious adverse event and patients’ rights should be regularly reviewed by an independent data monitoring committee (iDMC). ...patients would receive more or comparable benefit compared to the standard of care, without any risk of new drug/device failure. ...patients may benefit greatly from novel drugs/devices, but sometimes must take the risk of drug failure and potential adverse events.
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