Stigma limits life opportunities of persons with mental illness. Self-stigma, the internalization of negative stereotypes, undermines empowerment and could hinder recovery. Here we examined ...self-stigma’s effect on recovery among 222 disability pensioners with mental illness over 2 years, controlling for age, gender, symptoms and recovery at baseline measured by the Recovery Assessment Scale. More self-stigma at baseline was associated with a significant decrease in recovery after 1 year (not significant after 2 years). An increase of self-stigma from baseline to follow-up predicted less recovery 1 and 2 years later. Interventions that reduce self-stigma could therefore improve recovery.
Multiple proteins bind to telomeric DNA and are important for the role of telomeres in genome stability. A recent study established a broad-complex, tramtrack and bric-à-brac - zinc finger (BTB-ZF) ...protein, ZBTB10 (zinc finger and BTB domain–containing protein 10), as a telomeric variant repeat–binding protein at telomeres that use an alternative method for lengthening telomeres). ZBTB10 specifically interacts with the double-stranded telomeric variant repeat sequence TTGGGG by employing its tandem C2H2 zinc fingers (ZF1–2). Here, we solved the crystal structure of human ZBTB10 ZF1–2 in complex with a double-stranded DNA duplex containing the sequence TTGGGG to assess the molecular details of this interaction. Combined with calorimetric analysis, we identified the vital residues in TTGGGG recognition and determined the specific recognition mechanisms that are different from those of TZAP (telomere zinc finger–associated protein), a recently defined telomeric DNA–binding protein. Following these studies, we further identified a single amino-acid mutant (Arg767Gln) of ZBTB10 ZF1–2 that shows a preference for the telomeric DNA repeat TTAGGG sequence. We solved the cocrystal structure, providing a structural basis for telomeric DNA recognition by C2H2 ZF proteins.
Abstract
Recent observations of protoplanetary disks (PPDs) at submillimeter wavelengths have revealed the ubiquity of annular substructures that are indicative of pebble-sized dust particles trapped ...in turbulent ringlike gas pressure bumps. This major paradigm shift also challenges the leading theory of planetesimal formation from such pebbles by means of the streaming instability, which operates in a pressure gradient and can be suppressed by turbulence. Here, we conduct 3D local shearing box nonideal magnetohydrodynamic simulations of dust trapping in enforced gas pressure bumps, including dust backreaction. Under a moderate level of turbulence generated by the magnetorotational instability with ambipolar diffusion, which is suitable for outer disk conditions, we achieve quasi-steady states of dust trapping balanced by turbulent diffusion. We find strong dust clumping in all simulations near the gas pressure maxima, reaching a maximum density well above the threshold for triggering gravitational collapse to form planetesimals. A strong pressure bump concentrates dust particles toward the bump’s center. With a weak pressure bump, dust can also concentrate in secondary filaments off the bump’s center, due to dust backreaction, but strong clumping still occurs mainly in the primary ring around the bump’s center. Our results reveal dust-trapping rings to be robust locations for planetesimal formation in outer PPDs, while they may possess diverse observational properties.
Tert‐butylhydroquinone (TBHQ) has been widely used in oils and oleaginous foods as powerful antioxidants, and TBHQ can be easily converted to tert‐butylquinone (TQ) at room temperature. In this work, ...the changes in TBHQ and TQ contents in lard and soybean oil during storage are investigated in detail. Results show TBHQ content decreases gradually during storage as the TQ content increases in both lard and soybean oil at room temperature. Moreover, the decrement in TBHQ is much larger than the increment in TQ, and the difference increase over storage time. This suggests that TBHQ is transformed into other compounds in addition to TQ. To identify these compounds, lard containing 200 mg kg−1 of TBHQ stored for 150 days is extracted by methanol. Prep‐LC is used to purify the transformation products of TBHQ during storage. The products are identified as 2‐methylallyl‐hydroquinon and 2‐(2‐methoxy‐2‐methylpropyl)‐hydroquinone by LC/MS and NMR. The transformation mechanism of these products is also proposed.
Practical Applications: TBHQ is very prone to transformation due to its strong antioxidant activity. However, the transformation products of TBHQ in fats and oils may have an adverse effect on the shelf‐life and safety of lipid rich products. Moreover, the accuracy of TBHQ quantitative detection is also affected. The contents change of TBHQ, TQ, and other transformation products in lard and soybean oil containing 200 mg kg−1 TBHQ are investigated at room temperature. Apart from TQ, other transformation products of TBHQ are purified and identified. This work enriches the research on the transformation mechanism and products of TBHQ in lipid systems at room temperature. It provides better understanding on the safety and detection accuracy of TBHQ as well.
Lard and soybean oil containing 200 mg kg−1 tert‐butylhydroquinone (TBHQ) are stored for 150 days at room temperature. TBHQ and the transformation products of TBHQ are extracted by methanol and detected by NP‐HPLC. To identify the transformation products of TBHQ, Prep‐LC is used to isolate and purify the transformation products of TBHQ during storage. The products are identified as 2‐methylallyl‐hydroquinon and 2‐(2‐methoxy‐2‐methylpropyl)‐hydroquinone by LC‐MS and NMR. The transformation mechanism of these products is also proposed.
Lard and soybean oil containing 200 mg kg−1 tert‐butylhydroquinone (TBHQ) are stored for 150 days at room temperature. TBHQ and the transformation products of TBHQ are extracted by methanol and detected by NP‐HPLC. To identify the transformation products of TBHQ, Prep‐LC is used to isolate and purify the transformation products of TBHQ during storage. The products are identified as 2‐methylallyl‐hydroquinon and 2‐(2‐methoxy‐2‐methylpropyl)‐hydroquinone by LC‐MS and NMR. The transformation mechanism of these products is also proposed.
3-Alkyl-2-methoxypyrazines (MPs) are characteristic aroma compounds found in fragrant vegetable oils, a type of specially processed oils with enhanced flavor. MP contents in these oils are usually at ...trace level, which makes their quantification a big challenge. In this work, we describe an optimized approach with a double-step acid/alkali extraction for the analysis of such compounds, namely, 3-isopropyl-2-methoxypyrazine, 3-isobutyl-2-methoxypyrazine, and 3-sec-butyl-2-methoxypyrazine, in those fragrant oils using gas chromatography–tandem mass spectrometry (GC–MS/MS). The sample preparation conditions including selections and percentages of acids, alkalis, and extraction solvents, as well as the stability of MPs, were optimized and examined. Method validation was conducted with a good linearity (r 2 > 0.999), and average recoveries between 93.9 and 109.3% were achieved. The limit of detection ranged from 0.2 to 0.4 μg/kg, and the relative standard deviations varied from 0.4 to 12.2% for samples spiked with the MPs at different concentrations. Overall, the method satisfactorily meets the requirements for the measurement of trace-level MPs in the fragrant vegetable oils via odor activity value calculation, and the results indicate that the improved acid/alkali extraction method is suitable for the routine analysis of MPs in those vegetable oils.
Three analogues of aristololactam Ⅰ (AL Ⅰ), AL AⅡ, AL FⅠ and AL BⅡ, had been isolated from Houttuynia cordata, a commonly used medicinal and edible plant with heat-removing and toxin-removing ...functions. Considering the significant nephrotoxicity of AL Ⅰ, this study evaluated the toxicity of these three aristololactams (ALs) on human proximal tubular epithelial cells (HK-2) by MTT assay, ROS assay, ELISA tests and cytologic morphology observation. Furthermore, the distribution of the three ALs in H. cordata were investigated by UPLC-MSn recognition and quantitation in SIM mode, so as to estimate primarily the safety of the plant. The results showed that all the three ALs in H. cordata had comparative cytotoxicity as AL I with the IC50 values from 3.88 μM to 20.63 μM, caused high levels of cellular levels of reactive oxygen species (ROS) in HK-2 cells, exhibited the potential to cause renal fibrosis by remarkably increasing the levels of transforming growth factor-β (TGF-β1) and fibronectin (FN), and induced fibrous changes in morphology of HK-2 cells. The contents of the three ALs varied significantly in 30 batches of H. cordata from different regions and parts. Overall, the aerial part contained much more ALs (3.20 – 108.19 μg/g) than the underground part (0.95 – 11.66 μg/g), and flowers had the highest contents. Besides, no ALs were detected in the water extract of any part of H. cordata. This work revealed that the aristololactams in H. cordata had similar in vitro nephrotoxicity as AL Ⅰ and were mainly distributed in the aerial part of the plant.
Display omitted
•Aristolactams (ALs) in Houttuynia cordata showed toxicity to HK-2 cells.•The contents of 3 ALs in 30 batches of H. cordata were evaluated by UPLC-MSn.•The aerial part contained much more ALs than the underground part.
This paper investigates cryogenic temperature charge collection and single-event upset (SEU) in SiGe HBT devices and logic circuits using 3-D device simulation and circuit simulation. Cryogenic ...temperature circuit simulation is enabled by a new SiGe HBT compact model developed for wide temperature range operation. Incomplete ionization was found to impact charge collection below 130 K. With cooling, collector-substrate (CS) junction peak current I CS and integral charge Q CS first increase, and then decrease below 130 K. Circuit SEU immunity is found to be nearly independent of temperature above 150 K and improve with further cooling, suggesting no additional hardening is required for cryogenic temperature operation.
As ligand-gated ion channels, nicotinic acetylcholine receptors (nAChRs) are widely distributed in the central and peripheral nervous systems and are associated with the pathogenesis of various ...degenerative neurological diseases. Here, we report the results of phage display-based de novo screening of an 11-residue linear peptide (named LKP1794) that targets the α7 nAChR, which is among the most abundant nAChR subtypes in the brain. Moreover, two d-peptides were generated through mirror image and/or primary sequence inverso isomerization (termed DRKP1794 and DKP1794) and displayed improved inhibitory effects (IC50 = 0.86 and 0.35 μM, respectively) on α7 nAChR compared with the parent l-peptide LKP1794 (IC50 = 2.48 μM), which markedly enhanced serum stability. A peptide-based fluorescence probe was developed using proteolytically resistant DKP1794 to specifically image the α7 nAChR in living cells. This work provides a new peptide tool to achieve inhibitory modulation and specifically image the α7 nAChR.
...we speculated that ScCas9 could broaden the targeting scope of base editors considering the preference of NAG PAM. ...we constructed the hAID*Δ‐ScCas9n‐UGI‐NLS chimeric gene, cytidine base editor ...named rBE25 (Figure c), and tested its activity towards an NAG PAM at the OsBZR1 site in transgenic rice plants (Figure d). ...19 of 40 independent lines (47.5% efficiency) were identified with a single A to G conversion at the desired site. ...ScCas9 nuclease and its derived editing tools expand the CRISPR toolbox for targeted genome editing in plants.
In order to improve the digital transformation effect of enterprise accounting talents, this paper combines intelligent methods to carry out the digital transformation of enterprise accounting ...talents from the perspective of blockchain. Moreover, this paper studies the sliding window CS-SCHT algorithm in depth. Based on the theoretical derivation of the sliding window CS-SCHT based on the gray code kernel, the algorithm is implemented on the computer platform, and the test experiment of the operation time is carried out. In addition, this paper explores the application of the sliding window CS-SCHT algorithm in adaptive filtering. The experimental results show that the adaptive filter based on the CS-SCHT algorithm can obtain a higher signal-to-noise ratio than the sliding window DFT algorithm. Finally, this paper constructs an intelligent accounting digital information processing system. The research shows that the system proposed in this paper can play an important role in the digital transformation of enterprise accounting talents from the perspective of blockchain.
PDF
