Artificial intelligence (AI)-assisted clinical trial screening is a promising prospect, although previous matching systems were developed in English, and relevant studies have only been conducted in ...Western countries. Therefore, we evaluated an AI-based clinical trial matching system (CTMS) that extracts medical data from the electronic health record system and matches them to clinical trials automatically.
This study included 1,053 consecutive inpatients primarily diagnosed with hepatocellular carcinoma who were referred to the liver tumor center of an academic medical center in China between January and December 2019. The eligibility criteria extracted from two clinical trials, patient attributes, and gold standard were decided manually. We evaluated the performance of the CTMS against the established gold standard by measuring the accuracy, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and run time required.
The manual reviewers demonstrated acceptable interrater reliability (Cohen's kappa 0.65-0.88). The performance results for the CTMS were as follows: accuracy, 92.9-98.0%; sensitivity, 51.9-83.5%; specificity, 99.0-99.1%; PPV, 75.7-85.1%; and NPV, 97.4-98.9%. The time required for eligibility determination by the CTMS and manual reviewers was 2 and 150 h, respectively.
We found that the CTMS is particularly reliable in excluding ineligible patients in a significantly reduced amount of time. The CTMS excluded ineligible patients for clinical trials with good performance, reducing 98.7% of the work time. Thus, such AI-based systems with natural language processing and machine learning have potential utility in Chinese clinical trials.
Microcystin-leucine-arginine (MC-LR), which produced by toxic cyanobacteria and widely present in eutrophic waters, has been shown to have potent acute hepatotoxicity. MC-LR has been revealed to ...inflict damage to brain, while the neurotoxicity of chronic exposure to MC-LR and mechanisms underlying it are still confusing. Here, the mice were exposed to MC-LR dissolved in drinking water at dose of 1, 7.5, 15, and 30 μg/L for consecutive 180 days. MC-LR accumulated in mouse brains and impaired the blood-brain barrier by inducing the expression of matrix metalloproteinase-8 (MMP-8), which was regulated by NF-κB, c-Fos and c-Jun. Furthermore, MC-LR exposure induced microglial and astrocyte activation and resultant neuroinflammatory response. This study highlights the risks to human health of the current microcystin exposure.
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•MC-LR accumulated in mouse brains and impaired the blood-brain barrier by inducing the expression of MMP-8.•MC-LR increased levels of c-Fos, c-Jun and p65 to promote MMP-8 expression.•MC-LR exposure induced inflammation in brain, contributed to the activation of microglia and astrocyte.
Microplastic pollution is an issue of major environmental concern worldwide. Land-use type may affect the abundance, polymer types, and distribution characteristics of soil microplastics but their ...distribution remains unknown on the coastal plain of east China. Here, the abundance of microplastics in farmland (FL), plantation (P), and orchard/secondary forest (OSF) soils was determined on the east China coastal plain, and characteristics of the microplastics (shape, size, colour, and polymer composition) were analysed in soil samples collected from 33 sites. The average abundances of microplastics in FL, P, and OSF soils on the coastal plain of the east China coast were 185, 109, and 150 items kg−1, respectively. Small particles, fibres and transparent particles were the main characteristics of the microplastics observed. The polymer types were mainly PP and PET. The abundance of microplastics in farmland was positively correlated with population density in the study area. Therefore, agricultural activities associated with high population density are the main factors leading to the high abundance of microplastics in farmland soil.
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•Soil microplastics (MPs) were investigated in three land-use types on the coastal plain of east China.•The abundance of MPs was highest in farmland and lowest in plantations.•Abundance of MPs in farmland was positively correlated with the population density.•Small-sized, fibrous, and transparent particles were the main MP contaminants.•PP and PET were the main polymers in three land-use types.
Codewords used to determine fringe orders are significant for absolute phase retrieval based on the phase-coding method. However, because of inevitable interference from image blurring, image noise, ...and system nonlinearity, it is challenging to significantly increase the number of codewords for high-accuracy phase retrieval. To address this challenge, this article proposes a joint coding strategy to perform the coding of high-density stripes. More specifically, codewords are simultaneously embedded into the phase domain and the intensity domain of composite fringe patterns, which greatly extends the encoding range of valid codewords. Moreover, to improve the anti-interference capability of the proposed coding strategy, a regional correction technique (RCT) is presented to remove jump phase errors induced by the inevitable interference. The results of experiments indicate that the proposed method is effective and accurate for both complex objects and a standard sphere.
The metastasis‐associated lung adenocarcinoma transcription 1 (Malat1) is a long non‐coding RNA (lncRNA), exerts oncogenic role in multiple cancers, including hepatocellular carcinoma (HCC). This ...study was aimed to investigate its posttranscriptional regulation in HCC cells. RT‐PCR was performed to monitor the expression levels of Malat1 in normal liver and HCC cell lines. The expression of Malat1, microRNA (miR)‐195, and epidermal growth factor receptor (EGFR) in HepG2 and MHCC97 cells was respectively or synchronously altered by transfection. Then the changes in cell viability, apoptotic cell rate, cell cycle distribution, migration, and invasion were respectively assessed. As a result, we found that Malat1 was highly expressed in HCC cell lines when compared to normal liver cells. Malat1 silence suppressed HCC cells viability, migration and invasion, induced apoptosis, and arrested more cells in G0/G1 phase. Malat1 acted as a circular endogenous RNA (ceRNA) for miR‐195. Malat1 silence could not suppress HCC cell growth and motility when miR‐195 was knocked down. EGFR was a direct target of miR‐195. miR‐195 overexpression could not suppress HCC cell growth and motility when the 3′UTR site of EGFR was overexpressed. Furthermore, Malat1 silence blocked the activation of PI3K/AKT and JAK/STAT pathways, while EGFR overexpression activated them. Our study demonstrates Malat1‐miR‐195‐EGFR axis plays a critical role in HCC cells which provided a better understanding of Malat1 in HCC.
Malat1 silencing inhibits HCC cells growth and motility. MiR‐195 involves in Malat1‐mediaed carcinogenesis and plays an antitumor role via targeting the 3′UTR of epidermal growth factor receptor (EGFR). Malat1‐miR‐195‐EGFR axis plays a critical role in HCC cells.
Patients with inflammatory bowel disease (IBD) have a higher risk of developing colorectal cancer (CRC). Glycolysis is involved in the development of both IBD and CRC. However, the mechanisms and ...outcomes of glycolysis shared between IBD and CRC remain unclear. This study aimed to explore the glycolytic cross-talk genes between IBD and CRC integrating bioinformatics and machine learning. With WGCNA, LASSO, COX, and SVM-RFE algorithms, P4HA1 and PMM2 were identified as glycolytic cross-talk genes. The independent risk signature of P4HA1 and PMM2 was constructed to predict the overall survival rate of patients with CRC. The risk signature correlated with clinical characteristics, prognosis, tumor microenvironment, immune checkpoint, mutants, cancer stemness, and chemotherapeutic drug sensitivity. CRC patients with high risk have increased microsatellite instability, tumor mutation burden. The nomogram integrating risk score, tumor stage, and age showed high accuracy for predicting overall survival rate. In addition, the diagnostic model for IBD based on P4HA1 and PMM2 showed excellent accuracy. Finally, immunohistochemistry results showed that P4HA1 and PMM2 were significantly upregulated in IBD and CRC. Our study reveals the presence of glycolytic cross-talk genes P4HA1 and PMM2 between IBD and CRC. This may prove to be beneficial in advancing research on the mechanism of development of IBD-associated CRC.
Amidst the challenges of global environmental change and urbanization, the salutary effects of natural environments on public health are increasingly being recognized. This study investigates the ...specific effects of varied forest environments in China’s Wuyishan National Park on physiological and psychological health. Eight distinct forest environments were carefully selected, and a repeated-measures ANOVA approach was used to evaluate 41 participants over three days. Physiological assessments included Heart Rate Variability, Skin Conductance Level, and surface Electromyography, complemented by psychological evaluations using the Profile of Mood States. The key findings include the following: (1) Notable variations in physiological indicators were observed among different forest types. In valley tea gardens and broadleaf forest streamside, significant changes in heart rate indicators highlighted the influence of these settings on autonomic nervous activities. Skin Conductance Level and surface Electromyography also indicated varying emotional arousal and pleasure across the forests. The mixed broadleaf and coniferous forest valley, along with the rock-bedded streamscape, elicited emotions of low arousal but high pleasure, inducing feelings of calmness and pleasure. The valley’s tea gardens were associated with low arousal and pleasure, suggesting tranquility without positive emotional induction, while the broadleaf ridge forest induced high arousal and pleasure, reflecting an exciting and joyful environment. (2) The study found that different forest environments had a notable impact on participants’ mood states, indicating reductions in tension, anger, fatigue, and depression, along with an increase in vigor levels. In summary, forest environments offer unique psychological and physiological health benefits compared to urban settings. These findings underscore the importance of integrating forest environments into urban development and public health frameworks, and the need to further explore their impact on the health of diverse populations.
The Symposium ES1, Perovskite Solar Cells - Towards Commercialization, held at the 2017 Materials Research Society (MRS) Spring Meeting in Phoenix, Arizona (April 17-21, 2017) received ~200 ...abstracts. The 23 invited talks and 72 contributed oral presentations as well as 3 poster presentation sessions were organized into 13 principal themes according to the contents of the received abstracts. This Energy Focus article provides a concise summary of the opinions from the scientists and engineers who participated in this symposium regarding the recent progresses, challenges, and future directions for perovskite solar cells as well as other optoelectronic devices.
We present an n-alkane and compound-specific carbon isotope record of the past 9 ka from the annually laminated sedimentary sequence of Lake Xiaolongwan, northeastern China. The n-alkane distribution ...suggests that Lake Xiaolongwan has undergone a shift from an oligotrophic state with low algal production and little emergent/submerged macrophytes in the early Holocene, to a eutrophic state with high algal production and abundant emergent/submerged macrophytes since the middle Holocene. The pattern of variation observed in the biomarker proxies ACL (the n-alkane average chain length), Paq (aquatic macrophyte versus aquatic macrophyte and terrestrial plant ratio), and LPTP (lake productivity/terrigenous organic production) is throughout the record similar to that of the total organic carbon. The variation of compound-specific carbon isotopic values in the middle- and short-chain alkanes was mainly regulated by lake productivity and the accumulating organic pool through time. In this forested region, where the vegetation is dominated by C3 plants, the long-chain n-alkanes (C27–C31) are predominantly derived from leaf wax lipids. The compound-specific δ13C27–31 value is sensitive to effective precipitation, and therefore represents a useful indicator of regional monsoonal precipitation. Spectral analysis on the δ13C27–31 time series reveals significant periodicities of 87–89, 205–212, 1020–1050 and 1750–2041 years. On the centennial timescale, the quasi-periodicities around 88 and 210 years suggest a strong link between solar activity and monsoon rainfall. The millennial monsoon cycle in northeastern China is associated with sea surface temperature (SST) variations in two active centers of the summer monsoon, the western Pacific Subtropical High (WPSH) and the Okhotsk High. Increasing SST in the subtropical sea may cause a northwards shift of the WPSH, which extends the monsoon rain band (Meiyu) to northeastern China, and thus increasing rainfall in that region. Meanwhile, decreasing SST in the Okhotsk Sea may strengthen the Okhotsk high, bringing more moisture into northeastern China. We suggest that the Pacific Ocean is a main regulator for summer monsoon rainfall in northeastern China at present and at different time scales during the Holocene.
•An n-alkane and compound-specific carbon isotope record of the past 9 ka.•New idea about the n-alkane distribution and lake evolution.•The centennial monsoon oscillations could be linked to solar activity.•The millennial monsoonal cycles are associated with SST variations.•Pacific Ocean is a main regulator for monsoon rainfall in northeastern China.
Discoidin domain receptor 1 (DDR1) is a member of the receptor tyrosine kinase family, and its ligand is collagen. Previous studies demonstrated that DDR1 is highly expressed in many tumors. However, ...its role in hepatocellular carcinoma (HCC) remains obscure. In this study, we found that DDR1 was upregulated in HCC tissues, and the expression of DDR1 in TNM stage II-IV was higher than that in TNM stage I in HCC tissues, and high DDR1 expression was associated with poor prognosis. Gene expression analysis showed that DDR1 target genes were functionally involved in HCC metastasis. DDR1 positively regulated the migration and invasion of HCC cells and promoted lung metastasis. Human Phospho-Kinase Array showed that DDR1 activated ERK/MAPK signaling pathway. Mechanically, DDR1 interacted with ARF6 and activated ARF6 through recruiting PSD4. The kinase activity of DDR1 was required for ARF6 activation and its role in metastasis. High expression of PSD4 was associated with poor prognosis in HCC. In summary, our findings indicate that DDR1 promotes HCC metastasis through collagen induced DDR1 signaling mediated PSD4/ARF6 signaling, suggesting that DDR1 and ARF6 may serve as novel prognostic biomarkers and therapeutic targets for metastatic HCC.
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