Objective: To determine the presence of Helicobacter pylori in the middle ear effusion of patients with otitis media with effusion (OME) by polymerase chain reaction (PCR).
Study Design: A ...prospective study in patients with OME.
Methods: The study was performed in 38 patients with OME who were admitted to the ENT Clinic, Firat University from June 2003 to April 2004. In all cases, a myringotomy operation (with or without placement of a ventilation tube) was carried out. The effusion samples aspirated from the middle ear were analyzed with PCR assay.
Results: A total of 55 aspiration samples collected from 38 children ranging in age from 2 to 12 were included in the study. Fifteen of the subjects were girls, and 23 were boys. In 17 patients, both ears demonstrated effusions, whereas in 21 patients, only one ear had effusions. Nine (16.3%) of 55 the middle ear effusion samples were shown to be H. pylori positive by PCR.
Conclusions:
H. pylori was detected in the middle ear effusion of some patients with OME. These results may have interesting implications for a possible role of H. pylori in OME. In addition, these results suggest that further studies are needed to investigate the role of H. pylori in the etiology of OME.
Routine water analysis of arsenic species requires simple, inexpensive, rapid and sensitive methods. To this end, we have developed two methods, which are based on the use of inexpensive solid phase ...extraction (SPE) cartridges as low pressure chromatographic columns for separation and hydride generation atomic absorption spectrometry (HGAAS) and hydride generation atomic fluorescence spectrometry (HGAFS) for detection of arsenic. Both anion exchange and reverse phase cartridges were successfully used to separate arsenite As(III) and arsenate As(V). The composition, concentration, and pH of eluting buffers and the effect of flow rate were systematically investigated. Speciation of inorganic As(III) and As(V) were achieved within 1.5 min, with detection limits of 0.2 and 0.4 ng/ml, respectively. Both isocratic and step gradient elution techniques were suitable for the baseline resolution of As(III) and As(V) using anion exchange cartridges. Application of the methods to the speciation of As(III) and As(V) in untreated water, tap water, and bottled water samples were demonstrated. Results from the speciation of arsenic in a standard reference material water sample using these methods were in good agreement with the certified value and with inter-laboratory comparison results obtained using HPLC separation and inductively coupled plasma mass spectrometric detection (HPLC–ICPMS).
Olive (Olea europea L.) is one of the most economically relevant crops in Turkey. However, norwithstanding its importance, Turkey has no certification programme for olive and only a few studies of ...virus diseases of olive have been made using ELISA. Olive latent virus 1 (OLV-1, genus Necrovirus, family Tombusviridae) induces symptomless infections and is transmitted by mechanical inoculation. The virus was first isolated in Turkey from citrus trees affected by chlorotic dwarf disease (Martelli et al., 1996) but, to our knowledge, there are no records of OLV-1 in olive trees in Turkey. In this study, we tested 43 olive trees from Hatay province by RT-PCR. Total RNA was extracted from 0.1 g phloem tissue using RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA). Reverse transcription and PCR amplification, performed according to Faggioli et al. (2005), yielded amplicons of the expected size (ca. 299 bp) from two samples. The presence of OLV-1 was confirmed by sequencing each of the two amplicons spanning the capsid protein gene, and comparing the obtained sequences with those of OLV-1 in databases (Accession No. NC-001721, DQ083996 and AB061815). The two Turkish olive sequences were 99% identical to the citrus isolate sequence (NC-001721), 94% or 95% identical to the tulip isolate sequence (AB061815) and 93% identical to the olive isolate sequence (DQ083996). Homology was thus correlated more to the geographical origin than the host.
The standard model (SM) of particle physics is spectacularly successful, yet the measured value of the muon anomalous magnetic moment (g-2)μ deviates from SM calculations by 3.6σ. Several theoretical ...models attribute this to the existence of a “dark photon,” an additional U(1) gauge boson, which is weakly coupled to ordinary photons. For this study, the PHENIX experiment at the Relativistic Heavy Ion Collider has searched for a dark photon, U, in π0,η→γe+e- decays and obtained upper limits of θ(2×10-6) on U-γ mixing at 90% C.L. for the mass range 30<mU<90 MeV/c2. Lastly, combined with other experimental limits, the remaining region in the U-γ mixing parameter space that can explain the (g-2)μ deviation from its SM value is nearly completely excluded at the 90% confidence level, with only a small region of 29<mU<32 MeV/c2 remaining.
The purpose of this research is to determine whether prospective teachers' spiritual expressions have predicted their mindfulness. The research was conducted in relational screening model. The study ...group consisted of 411 students (81.2%) females and 94 (18.6%) males, totally 505 undergraduate students who are studying in the last year and who participated in teaching practice. For data collection procedure, the Mindful Awareness Scale, developed by Brown and Ryan was used. Turkish validity and reliability of the scale was studied by Özyesil, Arslan, Kesici and Deniz and the Spiritual Expression Scale (SES), developed by Macdonald. This scale was studied in Turkish with Turkish validity and reliability by Sahin and Baloglu. The results indicate that there is a positive meaningful relationship at nominal level between the mindfulness and the spiritual expression orientation with the religiosity sub-dimension and the spiritual cognitive orientation subscale; a negative meaningful relationship at nominal level between experiential phenomenological sub-dimensions; a positive meaningful relationship at moderate level between the existential well-being sub-dimensions. However, there is no meaningful relationship between mindfulness and paranormal beliefs sub-dimensions. As a result, it is seen that existential well-being sub-dimension of spiritual expression inventory predicts mindfulness.
Diagnostic ureterorenoscopy is used to identify upper tract urothelial cancer before radical nephro ureterectomy, especially for uncertain lesions in imaging modalities or urine cytology. However, ...diagnostic ureterorenoscopy can potentially cause intravesical tumor spillage and can increase intravesical recurrence rates. We aimed to investigate the impact of diagnostic ureterorenoscopy before radical nephroureterectomy, with and without biopsy, on intravesical recurrence rates of patients with upper tract urothelial cancer.
Patients with localized upper tract urothelial cancer from 8 different tertiary referral centers, who underwent radical nephroureterectomy between 2001 and 2020, were included. Three groups were made: no URS (group 1); diagnostic ureterorenoscopy without biopsy (group 2); and diagnostic ure terorenoscopy with biopsy (group 3). Intravesical recurrence rates and survival outcomes were compared. Univariate and multivariate Cox regression analyses were performed to determine the factors that were asso ciated with intravesical recurrence-free survival.
Twenty-two (20.8%), 10 (24.4%), and 23 (39%) patients experienced intravesical recurrence in groups 1, 2, and 3, respectively (P=.037) among 206 patients. The 2-year intravesical recurrence-free sur vival rate was 83.1%, 82.4%, and 69.2%, for groups 1, 2, and 3, respectively (P=.004). Cancer-specific survival and overall survival were comparable (P=.560 and P=.803, respectively). Diagnostic ureterore noscopy+biopsy (hazard ratio: 6.88, 95% CI: 2.41-19.65, P < .001) was the only independent predictor of intravesical recurrence in patients with upper tract urothelial cancer located in the kidney, according to tumor location.
Diagnostic ureterorenoscopy+biopsy before radical nephroureterectomy significantly increased the rates of intravesical recurrence in tumors located in kidney. This result suggests tumor spillage with this type of biopsy, so further studies with different biopsy options or without biopsy can be designed.
An experiment was conducted to determine the effect of divergent selection for tibial dyschondroplasia (TD) on live performance of broilers. Broilers used in the experiment were produced from the ...parental lines selected for high (H) and low (L) incidence of TD and a randombred control (C) line. Diallel crosses were made between H and L lines. The offspring produced were HH, HL, LH, LL, and CC, where the first letter refers to sire line and the second letter refers to dam line. Body weights, average daily body weight gains, and exponential growth rates were determined at weekly intervals. The incidence of TD was recorded at 4 and 7 wk of age. There was no difference among body weights of lines up to 5 wk of age. Sire lines influenced body weights of birds from 5 to 7 wk of age. A significant interaction between sire and dam lines for body weight was the result of decreased body weights of birds in the HH line from 5 to 6 wk of age. There was a similar interaction for body weight gain, which resulted in a slower growth rate of birds in the HH line from 3 to 5 wk of age. Tibial dyschondroplasia incidence was 84.1 and 92.0% in the HH line, 5.6 and 5.4% in the LL line, and 7.0 and 13.2% in the CC line at 4 and 7 wk of age, respectively. It was suggested that genetic predisposition for TD was independent of body weight.
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