Blood-sucking phlebotomine sand flies are vectors of the protozoan parasites Leishmania spp. Although the intestinal microbiota is involved in a wide range of biological and physiological processes ...and has the potential to alter vector competence, little is known about the factors that modify the gut microbiota composition of sand flies. As a key step toward addressing this issue, we investigated the impact of host species on the gut bacterial composition in Phlebotomus and Lutzomyia sand flies reared under the same conditions. Bacterial 16S rRNA gene amplification and Illumina MiSeq sequencing were used to characterize the overall bacterial composition of three laboratory-reared sandflies: Phlebotomus papatasi, Ph. duboscqi, and Lutzomyia longipalpis. Our results showed that the larvae of the three sand fly species harbored almost the same microbes but had different relative abundances. Adult Ph. papatasi and Ph. duboscqi revealed similar microbiome compositions, which were distinct from that of adult Lu. longipalpis. Furthermore, we showed that Ph. papatasi and Ph. duboscqi are hosts for different bacterial genera. The experiment was repeated twice to improve accuracy and increase reliability of the data, and the same results were obtained even when a distinct composition of the microbiome among the same species was identified probably because of the use of different larvae food batch. The present study provides key insights into the role of host species in the gut microbial content of different sand fly species reared under the same conditions, which may influence their susceptibility to Leishmania infection.
Injecting high-energy heavy ions in the electronic stopping regime into solids can create cylindrical damage zones called latent ion tracks. Although these tracks form in many materials, none have ...ever been observed in diamond, even when irradiated with high-energy GeV uranium ions. Here we report the first observation of ion track formation in diamond irradiated with 2-9 MeV C
fullerene ions. Depending on the ion energy, the mean track length (diameter) changed from 17 (3.2) nm to 52 (7.1) nm. High resolution scanning transmission electron microscopy (HR-STEM) indicated the amorphization in the tracks, in which π-bonding signal from graphite was detected by the electron energy loss spectroscopy (EELS). Since the melting transition is not induced in diamond at atmospheric pressure, conventional inelastic thermal spike calculations cannot be applied. Two-temperature molecular dynamics simulations succeeded in the reproduction of both the track formation under MeV C
irradiations and the no-track formation under GeV monoatomic ion irradiations.
Triterpenoids are the most representative group of phytochemicals, as they comprise more than 20,000 recognized molecules. These compounds are biosynthesized in plants via squalene cyclization, a C30 ...hydrocarbon that is considered to be the precursor of all steroids. Due to their low hydrophilicity, triterpenes were considered to be inactive for a long period of time; however, evidence regarding their wide range of pharmacological activities is emerging, and elegant studies have highlighted these activities. Several triterpenic skeletons have been described, including some that have presented with pentacyclic features, such as oleanolic and ursolic acids. These compounds have displayed incontestable biological activity, such as antibacterial, antiviral, and antiprotozoal effects, which were not included in a single review until now. Thus, the present review investigates the potential use of these triterpenes against human pathogens, including their mechanisms of action, via in vivo studies, and the future perspectives about the use of compounds for human or even animal health are also discussed.
Evidence points to a clear link between air pollution exposure and several chronic diseases though investigations regarding arthritis are still lacking. Emerging evidence suggests an association ...between ambient air pollution and rheumatoid arthritis. Household air pollution exposure, conversely, is largely unstudied but may be an important consideration for arthritis, particularly in low- and middle-income countries (LMICs), where cooking and heating activities can generate high indoor air pollutant levels.
We investigated the association of household air pollution (electricity vs. gas; kerosene/paraffin; coal/charcoal; wood; or agriculture/crop/animal dung/shrubs/grass as the main fuel used for cooking) and arthritis in six LMICs (China, Ghana, India, Mexico, the Russian Federation, South Africa) using data from Wave I of the World Health Organization Study on Global AGEing and Adult Health (SAGE) (2007-2010). Multivariable analyses were adjusted for sociodemographic, household and lifestyle characteristics and several comorbidities.
The use of gas (aOR = 1.76, 95%CI: 1.40-2.21); coal (aOR = 1.74, 95%CI: 1.22-2.47); wood (aOR = 1.69, 95%CI: 1.30-2.19); or agriculture/crop/animal dung/shrubs/grass: aOR = 1.95 (1.46-2.61) fuels for cooking were strongly associated with an increased odds of arthritis, compared to electricity in cluster and stratified adjusted analyses. Gender (female), age (≥50 years), overweight (25.0 ≤BMI<30.0 kg/m2), obesity (BMI ≥30.0 kg/m2), former and current alcohol consumption, and the comorbidities angina pectoris, diabetes, chronic lung disease, depression and hypertension were also associated with a higher odds of arthritis. Underweight (BMI<18.5 kg/m2) and higher education levels (college/university completed/post-graduate studies) were associated with a lower odds of arthritis.
These findings suggest that exposure to household air pollution from cook fuels is associated with an increased odds of arthritis in these regions, which warrants further investigation.
The paper presents mathematical modelling of steam condensation in the atmospheric air transonic flows. A single-fluid model was employed. The model was validated against the in-house experimental ...studies of internal flow in nozzles and against benchmark tests available in literature i.e., RAE2822 transonic airfoil. The impact of air relative humidity on the condensation process under transonic flow conditions was examined. The paper shows that the impact of the condensation process in air transonic flow is non-negligible and has to be taken under consideration in flow simulations. The presence of air contaminants in form of small particles was considered too. Their crucial role in the change of the transonic flow structure was numerically confirmed.
Data support the notion that 40-60% of patients with bipolar disorder (BD) have neurocognitive deficits. It is increasingly accepted that functioning in BD is negatively impacted by these deficits, ...yet they have not been a successful target for treatment. The biomarkers that predict cognitive deficits in BD are largely unknown, however recent evidence suggests that inflammation may be associated with poorer cognitive outcomes in BD. We measured C-reactive protein (CRP), a marker of systemic inflammation and risk of inflammatory disease, in 222 euthymic BD patients and 52 healthy controls. Within the patient sample, using multivariate analyses of covariance (MANCOVA) we compared cognitive performance of those with high CRP (≥5 mg/L) versus the remaining subjects (<5 mg/L) on a battery of cognitive tests. We evaluated relationships with several other relevant clinical features. We also examined the role of CRP in cognitive decline using a proxy cognitive decline metric, defined as the difference between premorbid and current IQ estimates, in a logistic regression analysis. Approximately 80% of our sample were BD-I, and the remainder were BD-II and 42.6% of our sample had a history of psychosis. We found a statistically significant effect of CRP on cognitive performance on a broad range of tests; participants with CRP ≥ 5 mg/L had worse performance on several measures of executive functioning, MATRICS processing speed and MATRICS reasoning and problem solving relative to those with lower CRP. We also identified CRP as a significant positive predictor of proxy cognitive decline. Our results indicate that elevated CRP is associated with a broad cognitive dysfunction in affectively remitted BD patients. These results may point to a subgroup of patients who might benefit from treatments to reduce inflammation.
Malaria is an important global public health challenge, and is transmitted by anopheline mosquitoes during blood feeding. Mosquito vector control is one of the most effective methods to control ...malaria, and population replacement with genetically engineered mosquitoes to block its transmission is expected to become a new vector control strategy. The salivary glands are an effective target tissue for the expression of molecules that kill or inactivate malaria parasites. Moreover, salivary gland cells express a large number of molecules that facilitate blood feeding and parasite transmission to hosts. In the present study, we adapted a functional deficiency system in specific tissues by inducing cell death using the mouse Bcl-2-associated X protein (Bax) to the Asian malaria vector mosquito, Anopheles stephensi. We applied this technique to salivary gland cells, and produced a transgenic strain containing extremely low amounts of saliva. Although probing times for feeding on mice were longer in transgenic mosquitoes than in wild-type mosquitoes, transgenic mosquitoes still successfully ingested blood. Transgenic mosquitoes also exhibited a significant reduction in oocyst formation in the midgut in a rodent malaria model. These results indicate that mosquito saliva plays an important role in malaria infection in the midgut of anopheline mosquitoes. The dysfunction in the salivary glands enabled the inhibition of malaria transmission from hosts to mosquito midguts. Therefore, salivary components have potential in the development of new drugs or genetically engineered mosquitoes for malaria control.
Context.
Star-forming molecular clouds are characterised by the ubiquity of intertwined filaments. The filaments have been observed in both high- and low-mass star-forming regions, and they are ...thought to split into collections of sonic fibres. The locations where filaments converge are termed hubs, and these are associated with the young stellar clusters. However, the observations of filamentary structures within hubs at distances of 75–300 pc require a high angular resolution <2″ (~ 150–600 au) that limits the number of such studies conducted so far.
Aims.
The integral shaped filament (ISF) of the Orion A molecular cloud is noted for harbouring several hubs within which no filamentary structures have been observed so far. The goal of our study is to investigate the nature of the filamentary structures within one of these hubs, which is the chemically rich hub OMC-2 FIR 4, and to analyse their emission with high density and shock tracers.
Methods.
We observed the OMC-2 FIR 4 proto-cluster using Band 6 of the Atacama Large (sub-)Millimetre Array (ALMA) in Cycle 4 with an angular resolution of ~0.26″ (100 au). We analysed the spatial distribution of dust, the shock tracer SiO, and dense gas tracers (i.e., CH
3
OH, CS, and H
13
CN). We also studied the gas kinematics using SiO and CH
3
OH maps.
Results.
Our observations for the first time reveal interwoven filamentary structures within OMC-2 FIR 4 that are probed by several tracers. Each filamentary structure is characterised by a distinct velocity as seen from the emission peak of CH
3
OH lines. They also show transonic and supersonic motions. SiO is associated with filaments and also with multiple bow-shock features. The bow-shock features have sizes between ~500 and 2700 au and are likely produced by the outflow from HOPS-370. Their dynamical ages are <800 yr. In addition, for the first time, we reveal a highly collimated SiO jet (~1°) with a projected length of ~5200 au from the embedded protostar VLA 15.
Conclusions.
Our study unveiled the previously unresolved filamentary structures as well as the shocks within OMC-2 FIR 4. The kinematics of the filamentary structures might be altered by external and/or internal mechanisms such as the wind from H II regions, the precessing jet from the protostellar source HOPS-370, or the jet from VLA 15. While the complexity of the region, coupled with the limited number of molecular lines in our dataset, makes any clear association with these mechanisms challenging, our study shows that multi-scale observations of these regions are crucial for understanding the accretion processes and flow of material that shape star formation.
Navigation surgery plays an important role in modern craniomaxillofacial surgery, but it is difficult to apply navigation surgery to the mandible, due to its mobility. At present, headbands or ...headpins are widely used for fixation of the reference frame, and three strategies are generally used for the application of navigation surgery to the mandible. This article reports the application of a novel open position splint integrated with a reference frame and registration markers for mandibular navigation surgery as a fourth strategy. Using this custom-made integrated splint, a marker-based pair-point registration procedure was completed easily and non-invasively. Furthermore, the neurovascular canal tract could be easily identified, and the cyst, as well as the surrounding daughter cysts, could be removed with high accuracy. This strategy has potential for widespread clinical application in mandibular navigation surgery.
Context.
Hot corinos are compact regions around solar-mass protostellar objects that are very rich in interstellar Complex Organic Molecules (iCOMs). How the abundance of these molecules is affected ...by the environmental physical conditions is still an open question. More specifically, addressing this point is key to understand our own chemical origins since the Solar System formed in a large cluster of low- to high-mass stars and was therefore subject to external heating and ultraviolet irradiation which may have shaped the chemistry of its early formation stages.
Aims.
The goal of this high resolution study is to determine the abundance ratios of iCOMs in HOPS-108, which is a Class 0 protostar and a hot corino candidate located in the nearest Solar System analogue, the protostellar cluster OMC-2 FIR 4, in Orion. We aim to compare the abundance ratios to those found in other hot corinos, which are all located in less crowded environments, in order to understand the impact of environmental conditions on hot corinos’ chemistry.
Methods.
We observed the OMC-2 FIR 4 proto-cluster using the Band 6 of the Atacama Large (sub-)Millimetre Array in Cycle 4 with an angular resolution of ~0.′′28 (110 au). We determined the abundances and temperature of the species using local thermodynamic equilibrium (LTE) and non-LTE analysis.
Results.
Our results reveal a rich organic chemistry towards HOPS-108, asserting that it is a hot corino where the following iCOMs are detected: CH
3
OH, HCOOCH
3
, CH
3
OCH
3
, CH
3
18
OH, CH
2
DOH, CH
3
COCH
3
, CH
3
CHO, CH
3
CN,
13
CH
3
CN, C
2
H
5
CN, and NH
2
CHO. Remarkably, we find a possible enhancement in the HCOOCH
3
abundance with respect to other known hot corinos. Indeed, the CH
3
OCH
3
/HCOOCH
3
abundance ratio in this source is ~0.2 and, within the uncertainties, it deviates from the known correlation marginally where CH
3
OCH
3
/HCOOCH
3
~1. A relatively low CH
2
DOH/CH
3
OH abundance ratio of ~0.02 is also obtained, which is in agreement with that found in another Orion source, HH212, suggesting a higher gas temperature during the early phases of ice mantle formation.
Conclusions.
The CH
3
OCH
3
/HCOOCH
3
and CH
2
DOH/CH
3
OH abundance ratios in HOPS-108 might result from different physical conditions in the Orion molecular complex compared to other regions. The former ratio cannot be reproduced with current chemical models, highlighting the importance of improving the chemical networks with theoretical calculations. More hot corinos located in heavily clustered regions such as Orion should be targeted in order to measure these ratios and evaluate whether they are an environmental product or whether HOPS-108 is an exceptional hot corino overall.
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