ZnO/ZnGa
2
O
4
composite microspheres with heterojunction were successfully synthesized by one-pot hydrothermal method. These samples were characterized by TG/DTA, XRD, TEM, HRTEM, UV-vis DRS, FL and ...BET techniques. The results indicated the as-prepared samples showed better degree of crystalline and large specific surface area. The photocatalytic activity was evaluated by degradation of methyl orange with the concentration of 50 mg/L under the irradiation of simulated sunlight. The effects of molar ratio of Zn to Ga and calcination temperature on the photocatalytic activity were investigated in detail. The results showed that the highest photocatalytic degradation efficiency was observed at the molar ratio of Zn to Ga of 1:0.5 in the starting materials and the calcination temperature of 400 °C. The maximum photocatalytic degradation rate of MO was 97.1% within 60 min under the simulated sunlight irradiation, which is greatly higher than that of ZnO and ZnGa
2
O
4
.
In this paper, a flux distribution model of the focal plane in dish concentrator system has been established based on ray tracking method. This model was adopted for researching the influence of the ...mirror slope error, solar direct normal irradiance, and tracking error of elevation-azimuth tracking device (EATD) on the focal spot characteristics (i.e., flux distribution, geometrical shape, centroid position, and intercept factor). The tracking error transmission law of the EATD transferred to dish concentrator was also studied. The results show that the azimuth tracking error of the concentrator decreases with the increase of the concentrator elevation angle and it decreases to 0 mrad when the elevation angle is 90°. The centroid position of focal spot along x-axis and y-axis has linear relationship with azimuth and elevation tracking error of EATD, respectively, which could be used to evaluate and calibrate the tracking error of the dish concentrator. Finally, the transmission law of the EATD azimuth tracking error in solar heliostats is analyzed, and a dish concentrator using a spin-elevation tracking device is proposed, which can reduce the effect of spin tracking error on the dish concentrator. This work could provide fundamental for manufacturing precision allocation of tracking devices and developing a new type of tracking device.
Two pairs of new phenolic enantiomers, (+)-eucophenolic A (1a), (-)-eucophenolic B (1b), (-)-eucophenolic C (2a), (+)-eucophenolic D (2b) were isolated from the leaves of Eucommia ulmodies Oliver by ...chiral enantiomeric resolution. Their structures were elucidated based on extensive spectroscopic analysis. The absolute configurations of 1a/1b and 2a/2b were determined by empirical method and the calculated ECD and OR. All compounds were tested for Hep G2 tumour cell lines. However, no compounds showed potential cytotoxic activities against Hep G2 in vitro.
To investigate the treatment methods used for the delayed interval delivery of twins and to evaluate the maternal and infant outcomes.
The clinical data of 5 patients that underwent delayed interval ...delivery of twins at Fujian Maternal and Child Health Hospital from 2014 to 2018 were analyzed. The gestational ages at delivery, obstetrical management, the interval between deliveries, and the maternal and child outcomes were analyzed.
The average gestational age at delivery of the first child was 23+3 weeks (range: 20+1–30+2 weeks). All 5 mothers underwent high ligation of the umbilical cord and received prophylactic antibiotic treatment. Tocolytics were administered to 3 patients, 1 of which had previously undergone cervical cerclage placement. No tocolytics were administered to the remaining 2 patients. The average delayed delivery time was 15 days (range: 3–31 days). The second child was delivered at an average gestational age of 25+5 weeks (range: 20+4–31+3 weeks). The average birth weight of the second twin was 957 g (range: 360–1930 g). Three of the patients delivered vaginally, 1 delivered via a cesarean section, and 1 required a breech extraction. Of these deliveries, there were 3 neonatal survivals. Pathogens were detected in the cervical secretion cultures in all cases. Two patients had grade 2 placental abruption, 5 had an intrauterine infection, 1 developed sepsis, 1 developed postpartum hemorrhage, and 5 showed a placenta adherence.
The delayed interval delivery of twins is a unique treatment for patients experiencing a twin pregnancy. Successful performance of this method can improve the survival rates for the second twin and improve prognosis. However, careful attention is required when performing this treatment to prevent and treat possible complications that may arise during the procedure.
In Traditional Chinese Medicine (TCM), most of the algorithms used to solve problems of syndrome diagnosis are superficial structure algorithms and not considering the cognitive perspective from the ...brain. However, in clinical practice, there is complex and nonlinear relationship between symptoms (signs) and syndrome. So we employed deep leaning and multilabel learning to construct the syndrome diagnostic model for chronic gastritis (CG) in TCM. The results showed that deep learning could improve the accuracy of syndrome recognition. Moreover, the studies will provide a reference for constructing syndrome diagnostic models and guide clinical practice.
For decades chlorine dioxide has been used in water disinfection with excellent results. As the scope of application expands, chlorine dioxide has the potential for soil disinfection. We used ...amplicon sequencing and gas chromatography-mass spectrometry to compare the changes of four mixed rhizosphere microbial community samples and 12 tobacco leaf volatile samples four months after the flood irrigation with chlorine dioxide in different concentrations (0, 2, 4, 8 mg/l). Phenotypic data of 60 tobacco plants were also collected. The effects of chlorine dioxide on rhizosphere microorganisms were positively correlated with dose gradients. Bacteria responded more strongly in both community structure and metabolic pathways than fungi. Five new bacterial phyla (Firmicutes, Bacteroidota, Myxococcota, Patescibacteria, Verrucomicroboata) appeared in chlorine dioxide treatment groups, while the fungal community only appeared as one new fungal phylum (Basidomycota). Alterations in 271 predicted metabolic bacterial pathways were found. However, in the fungal community were only 10 alternations. The correlations between leaf volatile compounds and rhizosphere microorganisms under the influence of chlorine dioxide treatment could be observed based on network results. However, natural connectivity had already been declining rapidly when less than 20% of the network's nodes were removed. Therefore, the microbe-metabolite network is not stable. It might be why chlorine dioxide treatments did not significantly affect tobacco quality (
= 0.754) and phenotype (
= 0.867). As a comprehensive investigation of chlorine dioxide in agriculture, this study proves the effectiveness and safety of chlorine dioxide soil disinfection and widens the application range of chlorine dioxide.
•Single nucleotide deletion of α-glucosidase causes the resistance in Culex quinquefasciatus.•The truncated α-glucosidase losses its binding ability to the binary toxin.•The cqm1R represents a new ...resistance allele in C. quinquefasciatus.
The entomopathogen Bacillus sphaericus is one of the most effective biolarvicides used to control the Culex species of mosquito. The appearance of resistance in mosquitoes to this bacterium, however, remains a threat to its continuous use in integrated mosquito control programs. Previous work showed that the resistance to B. sphaericus in Culex colonies was associated with the absence of the 60-kDa binary toxin receptor (Cpm1/Cqm1), an alpha-glucosidase present in the larval midgut microvilli. In this work, we studied the molecular basis of the resistance developed by Culex quinquefasciatus to B. sphaericus C3-41. The cqm1 genes were cloned from susceptible (CqSL) and resistant (CqRL/C3-41) colonies, respectively. The sequence of the cDNA and genomic DNA derived from CqRL/C3-41 colony differed from that of CqSL one by a one-nucleotide deletion which resulted in a premature stop codon, leading to production of a truncated protein. Recombinant Cqm1S from the CqSL colony expressed in Escherichia coli specifically bound to the Bin toxin and had α-glucosidase activity, whereas the Cqm1R from the CqRL/C3-41 colony, with a deletion of three quarters of the receptor’s C-terminal lost its α-glucosidase activity and could not bind to the binary toxin. Immunoblotting experiments showed that Cqm1 was undetectable in CqRL/C3-41 larvae, although the gene was correctly transcribed. Thus, the cqm1R represents a new allele in C. quinquefasciatus that confers resistance to B. sphaericus.
S-N inhibitors like thiourea and sewage sludge decomposition gases (SDG) are relatively novel dioxins suppressants and their efficiencies are proven in numerous lab-scale experiments. In this study, ...the suppression effects of both thiourea and SDG on the formation of dioxins are systematically tested in a pilot-scale system, situated at the bypass of a hazardous waste incinerator (HWI). Moreover, a flue gas recirculation system is used to get high dioxin suppression efficiencies. Operating experience shows that this system is capable of stable operation and to keep gaseous suppressant compounds at a high and desirable molar ratio (S + N)/Cl level in the flue gas. The suppression efficiencies of dioxins are investigated in flue gas both without and with addition of S-N inhibitors. A dioxin reduction of more than 80 % is already achieved when the (S + N)/Cl molar ratio is increased to ca. 2.20. When this (S + N)/Cl molar ratio has augmented to 4.18 by applying suppressant recirculation, the residual PCDD/Fs concentration in the flue gas shrank from 1.22 to 0.08 ng I-TEQ/Nm
3
. Furthermore, the congener distribution of dioxins is analysed to find some possible explanation or suppression mechanism. In addition, a correlation analysis between (S + N)/Cl molar ratios and PCDD/Fs is also conducted to investigate the chief functional compounds for dioxin suppression.
Overcoming tolerance to tumor-associated antigens remains a hurdle for cancer vaccine-based immunotherapy. A strategy to enhance the anti-tumor immune response is the inclusion of adjuvants to cancer ...vaccine protocols. In this report, we generated and systematically screened over twenty gene-based molecular adjuvants composed of cytokines, chemokines, and T cell co-stimulators for the ability to increase anti-tumor antigen T cell immunity. We identified several robust adjuvants whose addition to vaccine formulations resulted in enhanced T cell responses targeting the cancer antigens STEAP1 and TERT. We further characterized direct T cell stimulation through CD80-Fc and indirect T cell targeting via the dendritic cell activator Flt3L-Fc. Mechanistically, intramuscular delivery of Flt3L-Fc into mice was associated with a significant increase in infiltration of dendritic cells at the site of administration and trafficking of activated dendritic cells to the draining lymph node. Gene expression analysis of the muscle tissue confirmed a significant up-regulation in genes associated with dendritic cell signaling. Addition of CD80-Fc to STEAP1 vaccine formulation mimicked the engagement provided by DCs and increased T cell responses to STEAP1 by 8-fold, significantly increasing the frequency of antigen-specific cells expressing IFNγ, TNFα, and CD107a for both CD8
and CD4
T cells. CD80-Fc enhanced T cell responses to multiple tumor-associated antigens including Survivin and HPV, indicating its potential as a universal adjuvant for cancer vaccines. Together, the results of our study highlight the adjuvanting effect of T cell engagement either directly, CD80-Fc, or indirectly, Flt3L-Fc, for cancer vaccines.
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