The mandarin fish (Siniperca chuatsi) is a traditionally cultured freshwater fish with high commercial value in China. To facilitate marker-assisted selection in genetic improvement of this species, ...120 microsatellite markers from the literature were characterized in the 25 largest and 25 smallest individuals. Eighteen polymorphic loci were then used to genotype 200 individuals, and the associations between their genotypes and growth traits were examined. We found that eight genotypes of six loci (AP 37-06, AP 37-11, AP 37-16, AP 37-48, AP 38-32, and AP 39-05) were positively correlated with growth traits (body weight, length, and height) in the mandarin fish population. The average observed and expected heterozygosities were 0.68 and 0.59, respectively, and the average PIC value was 0.50, indicating a population with high genetic diversity. Therefore, these markers could be useful for assisted selection in genetic breeding of this species and its related species.
Abstract
A novel method, noted as approximate envelope method (AEM), was developed to estimate the secondary PM
2.5
concentrations based on the air quality monitoring data. This approach made it ...possible to obtain the long‐term characteristics of the secondary PM
2.5
using only conventional observations. The secondary PM
2.5
concentrations and their variation characteristics under different PM
2.5
pollution levels during wintertime of 2015 to 2018 in Chengdu, a major urban area of Sichuan Basin over Southwest China, were analyzed. The results showed that the secondary PM
2.5
concentrations ranged from 52.3 to 122.8 μg·m
−3
and accounted for 56.3%, 63.6%, and 67.4% of the total PM
2.5
in slight, moderate, and heavy pollution status, respectively. Additionally, the concentrations of secondary PM
2.5
increased year by year, while the concentrations of primary PM
2.5
performed significant decreasing trends from 2015 to 2018. The diurnal variations of primary and secondary PM
2.5
presented a significantly unimodal patterns but with maximum at morning and noon, respectively. Specific meteorological conditions such as high (low) relative humidity and temperature (wind speed and air pressure) aggravated the secondary particulate pollution in wintertime of Chengdu, reflecting an influence of regional climate change over the Sichuan Basin of Southwest China on the urban air pollution.
Key Points
The PM
2.5
/CO ratio is a valid tracer for estimating the concentration of primary particles
Secondary particles dominated wintertime PM
2.5
in urban area of Chengdu
To investigate the effects of early rehabilitation training after total knee arthroplasty surgery by continuous femoral nerve block (CFNB) with or without periarticular local infiltration analgesia ...(PLIA).
In this randomized, double-blind, controlled study, 100 patients under-going primary unilateral total knee arthroplasty in patients with knee osteoarthritis were enrolled. All the patients received CFNB for postoperative analgesia before combined spinal epidural anesthesia. They were randomly divided into 2 groups (n=50 each): CFNB group, CFNB combined with PLIA group (PLIA group). Group PLIA received periarticular local infiltration analgesia with 20 mL ropivacaine (5 g/L), while the equal volume of normal saline was used instead of ropivacaine in group CFNB. Postoperative pain during rest and passive exercises including front and rear portions of knees, the time of ability to perform an active straight leg raise, the time of ability to reach 90° knee flexion, and preoperative and postoperative hospital fo
The magnetic equivalent (MMNm) of mismatch negativity may reflect auditory discrimination and sensory memory. To study whether temporal lobe epilepsy (TLE) affects automatic central auditory-change ...processing, we recorded magnetoencephalographic (MEG) responses to standard and duration-deviant sounds in 12 TLE patients and 12 age-matched controls, and repeated MEG measurement in 8 patients 6–30 months following epilepsy surgery and in 6 controls 3–8 months after their first measurement. We compared the MMNm between patients and controls, and also evaluated intertrial phase coherences as indexed by phase-locking factors (PLF) using wavelet-based analyses. We observed longer MMNm latencies for patients than for controls. Dipole modeling and minimum-current estimates together showed bi-frontotemporal sources for MMNm. The phase locking across trials was dominant at the 4- to 14-Hz band, and the main difference in PLF between deviant- and standard-evoked responses occurred in the time frame of 150–250 ms after stimulus onset. Notably, in the 5 patients who became seizure free after removal of right temporal epileptic focus, the phase-locking phenomena resulting from deviant stimuli were enhanced, and even more distributed in the frontotemporal regions. We conclude that mesial TLE might affect auditory-change detection, and a successful surgery causes a possible plastic change in phase locking of deviant-evoked signals.
Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in ...plant phylogenetic analyses is often inadequate. To identify conserved regions that can be used to design primers and amplify variable sequences for use in plant phylogenetic studies, the complete chloroplast genomic sequences of six plant species (including Oryza sativa, Arabidopsis thaliana, Glycine max, Lotus japonicus, Medicago truncatula, and Phaseolus vulgaris), searched from the taxonomy database of NCBI were investigated. A total of 93 conserved regions, 32 in large single copy and 61 in inverted repeat regions, were identified. A set of five primer pairs were designed according to the conserved sequences located in the psbA~trnK, psbB~psbH, rpl23~trnI, trnR~trnN, and trnY~trnD regions to amplify variable DNA fragments. An additional 18 plant accessions from 14 species were used to validate their utility. Each of the tested species could be distinguished by length polymorphisms of fragments amplified with the five primer pairs. trnR~trnN and rpl23~trnI amplified fragments specific to monocot and legume species, respectively. Three primer pairs located in the psbA~trnK, psbB~psbH, and trnR~trnN regions were applied to amplify variable DNA sequences for phylogenetic analysis using the maximum parsimony method. The consistent result between taxonomy and phylogenetic analysis on the variable sequences amplified with these three primer pairs was revealed. The five newly developed primer pairs are recommended as tools for use in the identification of plant species and in phylogenetic studies.
Chronic Helicobacter pylori-stimulated immune reactions determine the pathogenesis of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. We aimed to explore the genetic predisposition to this ...lymphoma and its clinical implication. A total of 68 patients and 140 unrelated controls were genotyped for 84 single-nucleotide polymorphisms in genes encoding cytokines, chemokines and related receptors that play important roles in T cell-mediated gastrointestinal immunity. Five genotypes in IL-22, namely CC at rs1179246, CC at rs2227485, AA at rs4913428, AA at rs1026788 and TT at rs7314777, were associated with disease susceptibility. The former four genotypes resided in the same linkage disequilibrium block (r(2)=0.99) that conferred an approximately threefold higher risk. In vitro experiments demonstrated that co-culturing peripheral mononuclear cells or CD4(+) T cells with H. pylori stimulated the secretion of interleukin-22 (IL-22), and that IL-22 induced the expression of antimicrobial proteins, RegIIIα and lipocalin-2, in gastric epithelial cells. Furthermore, patients with gastric tissue expressing IL-22 were more likely to respond to H. pylori eradication (14/22 vs 4/19, P<0.006). We conclude that susceptibility of gastric MALT lymphoma is influenced by genetic polymorphisms in IL-22, the product of which is involved in mucosal immunity against H. pylori and associated with tumor response to H. pylori eradication.
In this paper, a robust analog design (ROAD) tool for post-tuning (i.e., locally optimizing) analog/RF circuits is proposed. Starting from an initial design derived from hand analysis or analog ...circuit optimization based on simplified models, ROAD extracts accurate performance models via transistor-level simulation and iteratively improves the circuit performance by a sequence of geometric programming steps. Importantly, ROAD sets up all design constraints to include large-scale process and environmental variations, thereby facilitating the tradeoff between yield and performance. A crucial component of ROAD is a novel projection-based scheme for quadratic (both polynomial and posynomial) performance modeling, which allows our approach to scale well to large problem sizes. A key feature of this projection-based scheme is a new implicit power iteration algorithm to find the optimal projection space and extract the unknown model coefficients with robust convergence. The efficacy of ROAD is demonstrated on several circuit examples
A new heavy-ion linac within a continuous-wave (CW) 4-rod radio-frequency quadrupole (RFQ) was designed and constructed as the injector for the separated-sector cyclotron (SSC) at the Heavy Ion ...Research Facility at Lanzhou (HIRFL). In this paper, we present the development of and the beam commissioning results for the 53.667 MHz CW RFQ. In the beam dynamics design, the transverse phase advance at zero current, σ0⊥ , is maintained at a relatively high level compared with the longitudinal phase advance (σ0∥ ) to avoid parametric resonance. A quasi-equipartitioning design strategy was applied to control the emittance growth and beam loss. The installation error of the electrodes was checked using a FARO 3D measurement arm during the manufacturing procedure. This method represents a new approach to measuring the position shifts of electrodes in a laboratory environment and provides information regarding the manufacturing quality. The experimental results of rf measurements exhibited general agreement with the simulation results obtained using CST code. During on-line beam testing of the RFQ, two kinds of ion beams (Ar408+ and O165+ ) were transported and accelerated to 142.8keV/u , respectively. These results demonstrate that the SSC-Linac has made a significant progress. And the design scheme and technology experiences developed in this work can be applied to other future CW RFQs.
UHRF1BP1 encodes a highly conserved protein with unknown function. Previously, a coding variant in this gene was found to be associated with systemic lupus erythematosus (SLE) in populations of ...European ancestry (rs11755393, R454Q, P=2.22 x 10⁻⁸, odds ratio=1.17). In this study, by a combination of genome-wide study and replication involving a total of 1230 patients and 3144 controls, we confirmed the association of this coding variant to SLE in Hong Kong Chinese. We also identified another coding variant in this gene that independently contributes to SLE susceptibility (rs13205210, M1098T, P=4.44 x 10⁻⁹, odds ratio=1.49). Cross-population confirmation establishes the involvement of this locus in SLE and indicates that distinct alleles are contributing to disease susceptibility.
Abstract
We report the discovery of a new unidentified extended
γ
-ray source in the Galactic plane named LHAASO J0341+5258 with a pretrial significance of 8.2 standard deviations above 25 TeV. The ...best-fit position is R.A. = 55.°34 ± 0.°11 and decl. = 52.°97 ± 0.°07. The angular size of LHAASO J0341+5258 is 0.°29 ± 0.°06
stat
± 0.°02
sys
. The flux above 25 TeV is about 20% of the flux of the Crab Nebula. Although a power-law fit of the spectrum from 10 to 200 TeV with the photon index
α
= 2.98 ± 0.19
stat
± 0.02
sys
is not excluded, the LHAASO data together with the flux upper limit at 10 GeV set by the Fermi-LAT observation, indicate a noticeable steepening of an initially hard power-law spectrum with a cutoff at ≈50 TeV. We briefly discuss the origin of ultra-high-energy gamma rays. The lack of an energetic pulsar and a young supernova remnant inside or in the vicinity of LHAASO J0341+5258 challenge, but do not exclude, both the leptonic and hadronic scenarios of gamma-ray production.
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