This study was carried out to evaluate the loss of heterozygosity (LOH) in the 8q12-q24.1 chromosomal region, containing RAD54B gene in breast cancer. Polymorphic markers D8S539 and D8S543 were used. ...For alleles frequency estimation 100 primary breast cancers were tested. DNA was isolated from paraffin-embedded tissues and their matched blood samples. Polymerase chain reaction amplified products of normal and tumor DNA pairs were compared in ABI PRISM 377 DNA sequencer. In analyzed cases LOH was found in 1% and 2% of informative cases for microsatellite markers D8S539 and D8S543, respectively. This date indicate, that LOH isn't predictive for breast cancer.
This study was carried out to evaluate the loss of heterozygosity (LOH) and microsatellite instability (MSI) in breast cancer, in the 12p13.3 and 1p32 chromosomal regions where RAD52 and RAD54 genes ...are localized. Polymorphic markers D12S98, D12S1698 for RAD52 and D1S209, D1S411 for RAD54 were used. Relationships between LOH and clinicopathological parameters, i.e. tumor type and grade, patient's age, steroid receptors status and lymph node and distal metastases were assessed. For alleles frequency estimation 100 primary breast cancers were tested. DNA isolated from paraffin-embedded tissues and their matched blood samples were analyzed for PCR-based LOH and MSI by fluorescence-based DNA sequencing technology. In analyzed cases LOH was found in 14% and 11% of informative cases for D12S98 and D12S1698 markers, respectively and in 18% and 17% of informative cases for D1S209 and D1S411 markers, respectively. The highest frequency of MSI was identified at loci D12S98 (10%) and D1S209 (11%). Significant correlations between RAD52 and RAD54 regions with concomitant LOH and histological type and progesterone receptor status were observed. In the case of RAD54 further correlations with respect to tumor grade and the presence of distal metastases were noticed.
The authors describe a tonsillolith detected by a 70-year-old man with odynophagy and a history of often tonsillar infections. A diagnosis of peritonsillar abscess was first made. The large ...concrement was yellowish-gray, measured 41 x 21 x 19 mm, which was one of the largest reported case in the world.
Branchiogenic carcinoma is a rare condition and should not be diagnosed in the absence of the criteria laid down by Dr Hayes Martin. The authors describe a 65 year-old woman with a cystic mass on the ...side of the neck. The woman was admitted to the hospital for possible branchial cyst. The histopathological analysis showed a carcinoma developed on a branchial cyst. The authors stress the importance of careful and repeated clinical examinations, which exclude the possibility of a primary tumor of other localization.
The authors have studied the effect of azelastine nasal spray in the treatment of seasonal allergic rhinitis. The trial was a double-blind, placebo controlled study, with 33 allergic subjects (17 ...female, 16 male), 15-40 years old. The patients were randomized into two parallel groups, to receive daily 0.56 mg of azelastine intranasally or placebo during two weeks. Before and after treatment severity of nasal symptoms such as: sneezing, itching, nasal blockage, nasal discharge and general feeling were evaluated by patients according to VAS (visual analogue scale). At the same time the physician's evaluation of nasal oedema, nasal discharge and general patient's condition were performed (VAS). Additionally during the treatment patients noted possible adverse events. Patient's and physician's evaluations showed clinical efficacy in the azelastine group and no evident efficacy in the placebo group--between those two groups statistical significances were noted for all evaluated parameters (p < 0.001). The bitter taste in the mouth was the only serious side effect in some patients in azelastine group, although this was quite well accepted. Generally the treatment was well-tolerated. Azelastine nasal spray is an effective and well-tolerated drug in the treatment of seasonal allergic rhinitis.
The authors describe a case of a man with a large choanal polyp descending into the hypopharynx. The man presented additional symptoms of full aspirin intolerance and pollinosis caused with grass ...pollen antigens. The Caldwell-Luc procedure was performed and the choanal polyp was successfully removed through the mouth. Postoperatively treatment with leukotriene antagonists and with an intranasal steroids with positive clinical effect was begun. However the patient discontinued the therapeutic process causing polyp recurrence within a short time.
Sarcoidosis is a disease of unknown etiology characterized by non-caseating granulomatous inflammation of various organs, but most frequently involving the lungs of young adults. Sarcoidosis is ...seldom seen by ear, nose, throat and larynx. The isolated case of sarcoidosis of the larynx is very rare. We present a case of histologically confirmed sarcoidosis of the vocal cord. The clinical aspects, diagnostic problems and therapeutical possibilities of this rare disease are described.
Natural gamma background dose rates 1 m above ground level were evaluated using MCP-N detectors (LiF:Mg,Cu,P, produced at INP Kraków) at over 30 points, and the natural radioactivity of samples of ...materials deposited at the Przezchlebie industrial stockpile gathered from some of these locations assessed off-site using gamma ray spectrometry. Measurements were made in late spring over different parts of the stockpile and the neighbouring terrain, and repeated after one year. Above-ground gamma dose rates correlated well with the activity of elements in the 232Th and 238U series, the 226Ra sub-series and 40K measured in the material samples. LiF:Mg,Cu,P (MCP-N) detectors are extremely convenient for use in environmental dosimetry as their stability and sensitivity allow field exposures to take no longer than two weeks, instead of three months required for LiF:Mg,Ti (TLD-100) detectors. This considerably speeds up measurements and may prevent theft or displacement of dosemeters in industrial conditions.
PDF
