The mechanism of regulatory phosphorylation of the pyruvate dehydrogenase component (E1) of muscle pyruvate dehydrogenase complex was studied. Chemical modification of the arginine residues essential ...for substrate binding was shown to prevent incorporation of
32P from γ-
32PATP into E1 catalyzed by kinase and to exclude completely the interaction of holo-E1 with pyruvate. It is proposed that negatively charged phosphoseryl residues may compete with pyruvate for the active site arginine and thereby block the substrate binding.
Individual brains vary in both anatomy and functional organization, even within a given species. Inter-individual variability is a major impediment when trying to draw generalizable conclusions from ...neuroimaging data collected on groups of subjects. Current co-registration procedures rely on limited data, and thus lead to very coarse inter-subject alignments. In this work, we present a novel method for inter-subject alignment based on Optimal Transport, denoted as Fused Unbalanced Gromov Wasserstein (FUGW). The method aligns cortical surfaces based on the similarity of their functional signatures in response to a variety of stimulation settings, while penalizing large deformations of individual topographic organization. We demonstrate that FUGW is well-suited for whole-brain landmark-free alignment. The unbalanced feature allows to deal with the fact that functional areas vary in size across subjects. Our results show that FUGW alignment significantly increases between-subject correlation of activity for independent functional data, and leads to more precise mapping at the group level.
The objective of the present work was to search for methodological options facilitating the improvement and optimization of the biological microchip designed for genotyping of the AB0 locus. Testing ...a prototype biological microchip for genotyping of the AB0 locus using a reference set of preparations with the known group specificity has demonstrated that the choice of DNA probes by theoretical calculation of their thermodynamic parameters does not necessarily yields the desired practical result. Suffice it to say that under certain conditions some cells of the biochip appear to generate artifact hybridization signals that tend to make the results of genotyping either incorrect or difficult to interpret. This problem required the adjustment of the molecular structure of DNA probes for the optimization of their hybridization properties. As a result new DNA probes have been developed and synthesized and new variants of the prototype biochip constructed to be subjected to experimental verification.
Aim: Figuring out the occurrence of vestibular schwannomas (VS) and their management tendencies in Ukraine.Materials and methods: Data about VS detection and treatment were collected among all ...Ukrainian neurosurgical and radiological facilities which are enrolled in providing care for these patients. Together with those who were treated overseas the total number in 2016-2018 was 903 people. There were 665 cases (73.6 %) treated surgically, 124 cases (13.8 %) irradiated and 114 (12.6 %) observed via serial imaging.Results: The prevalence of VS in Ukraine is about 7.27 per 1 million people which corresponds to data around the world (CBTRUS trial suggested VS incidence 10-20 people per 1 million during 2004-2009).Most of verified VSs were treated surgically (73.6 %), lesser part was irradiated (13.8 %) and 12.7 % were followed-up by wait-and-scan strategy. Our data regarding surgical management was higher than worldwide. At the same time, the volume of detected tumors was much larger in comparison to published data. Seventy-three per cent of all cases were Koos T4 tumors as a possible result of poor diagnosis and lack of alertness making surgical interventions more common and difficult.Total and subtotal resection rate was 79 % as the result of combined microsurgical and endoscopic techniques under intraoperative electrophysiological neuromonitoring guidance. The facial nerve was preserved in 94.2 % of cases, cochlear — in 8.5 % of cases. The average mortality rate during 2016-2018 in Ukraine was 3.1 % with 1.3 % in Subtentorial Neurooncology Department of the Romodanov Neurosurgery Institute.Conclusions: For further improvements and development of optimal management strategies for patients with VS, it is necessary to improve earlier diagnosis and reasonable to provide neurosurgical care in high-volume centers based on the profound expertise with further advances in technologies for functionally favorable outcomes.
Practical aspects concerning standardization of molecular-genetic expertise performing with the use of the method of DNA are considered. Examples of difficulties, which can occur at nonobservance of ...requirements of polymerase chain reaction and electrophoresis performing, are described; practical recommendations of their elimination are given.
Changes in the electrochemical properties and morphology of the surface of fibrous materials in the course of their modification with chitosan were studied in relation to the conditions of biopolymer ...deposition. The capacitances of the electrical double layer of the chitosan-carbon materials in NaCl solutions of various concentrations were determined by of cyclic voltammetry and the method of charging curves. The parameters of charging of the modified electrodes were calculated.
A novel mixed-ligand complex ZnPhen(i-Pr
2NCS
2)
2 has been synthesized and its single crystals grown. We have determined its crystal structure from X-ray diffraction data. The structure consists of ...monomeric molecules; a zinc atom surrounded by two nitrogen and four sulfur atoms making a distorted octahedron. Various packing modes of molecules forming isolated ‘dimers’, ribbons (chains), columns and layers have emerged from the study of the spatial molecule arrangement in the complexes ZnPhen(R
2NCS
2)
2 (R=Me, Et, i-Pr, n-Pr, i-Bu, n-Bu). The interactions between the Phen molecules have also been studied for these complexes.
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