This paper presents a new transfer function approach for calculating both the average temperature and hot spot temperature of an induction machine with the consideration of temperature-dependent ...material properties and speed-dependent heat transfer coefficient. Green's functions are introduced and taken as the new transfer functions. Online thermal monitoring models for induction machines are obtained based on Green's function method. Using the presented method, the hot spot temperature and average temperature can be calculated accurately and rapidly. The proposed method is validated via finite-element method and experimental measurement.
This paper proposes a novel outer-rotor flux-switching permanent-magnet (OR-FSPM) machine with specific wedge-shaped magnets for in-wheel light-weight traction applications. First, the geometric ...topology is introduced. Then, the combination principle of stator slots and rotor poles for OR-FSPM machines is investigated. Furthermore, to demonstrate the relationship between performance specifications (e.g., torque and speed) and key design parameters and dimensions (e.g., rotor outer diameter and stack length) of OR-FSPM machines at preliminary design stage, an analytical torque-sizing equation is proposed and verified by two-dimensional (2-D) finite-element analysis (FEA). Moreover, optimizations of key dimensions are conducted on an initially designed proof-of-principle three-phase 12-stator-slot/22-rotor-pole prototyped machine. Then, based on 2-D-FEA, a comprehensive comparison between a pair of OR-FSPM machines with rectangular- and wedge-shaped magnets and a surface-mounted permanent-magnet (SPM) machine is performed. The results indicate that the proposed OR-FSPM machine with wedge-shaped magnets exhibits better flux-weakening capability, higher efficiency, and wider speed range than the counterparts, especially for torque capability, where the proposed wedge-shaped magnets-based one could produce 40% and 61.5% more torque than the rectangular-shaped magnets-based machine and SPM machine, respectively, with the same rated current density (5 A/mm 2 ). Finally, the predicted performance of the proposed OR-FSPM machine is verified by experiments on a prototyped machine.
Fibroblast growth factor receptor 4 (FGFR4) has been indicated as a potential “oncogene” in various types of cancer. However, the effects and underlying mechanisms of FGFR4 on uterine leiomyosarcoma ...(ULMS) progression remain unclear. In this study, we firstly discovered that FGFR4 was upregulated in ULMS specimens and cell lines and closely associated with poor prognosis of ULMS patients. Cell viability and apoptosis assays showed that FGFR4 deletion inhibited cell proliferation and promoted cell apoptosis. Moreover, FGFR4 silence increased cytoplasmic GABP (GA binding protein) expression, while it decreased the nuclear GABP level to inhibit nuclear localization of GABP. Mechanistically, the inhibition ability of FGFR4 silence on nuclear localization of GABP was mediated via mammalian Ste20-like kinases 1 (MST1) activation, which could promote phosphorylation of large tumor suppressor 1 (LATS1) to reduce nuclear localization of GABP. Gain- and loss-of-functional assays indicated that FGFR4 promoted nuclear localization of GABP to inhibit cell apoptosis in ULMS. In conclusion, our findings indicated that FGFR4 inhibited cell apoptosis in ULMS via the promotion of MST1/LATS1-mediated GABP nuclear localization, shedding light on the underlying mechanism of FGFR4-induced ULMS progression.
Flux-switching permanent magnet machine typically suffers from relatively high cogging torque due to its special doubly salient structure and high air-gap flux density, causing undesired torque ...ripples, as well as acoustic noise and vibration, especially at low speeds. In this paper, an analytical expression of cogging torque is derived based on a magnetomotive force-permeance model, through which the optimal design parameters and dimensions, such as combinations of stator slots and rotor poles, skewing angle, stator tooth width, rotor tooth width, and magnet thickness, can be determined. Based on the derived analytical model, two novel approaches for cogging torque reduction are proposed, i.e., asymmetric magnetomotive force and asymmetric permeance, respectively, and consequently, the optimal designs are also acquired analytically. However, it is also found that the optimal choice of the proposed techniques depends on the combinations of stator slots and rotor poles of the machines. Finally, the effectiveness of the proposed approaches is verified by both finite element analytical predictions and experimental results.
In this paper, a novel outer-rotor-permanent-magnet flux-switching (ORPMFS) machine is proposed and analyzed. First, the geometric topology of the ORPMFS machine is introduced. Then, the PM-excited ...magneto motive force and open-circuit air-gap flux density are analyzed. Throughout the analysis, the ORPMFS machine is found to have the same air-gap flux density waveform as surface-mounted permanent magnet machine. Furthermore, the analytical equation of the back-electromotive force in the ORPMFS machine is deduced. Then, based on the analytical equation, different combinations of stator slot and rotor pole numbers are evaluated. Finally, the analytical results are verified by 2-D finite element analysis results.
With the purpose of quantifying the differences in the running performance of soccer players during matches from different continental confederations, data of 1508 match observations generated from ...559 players in 59 matches at the 2018 FIFA World Cup held in Russia were analyzed. Generalized mixed linear modeling was carried out to estimate the effect of confederations on each of the selected thirteen match running performance related variables (total distance covered, top speed achieved, number of sprints, distance covered and time spent in walking, jogging, low-speed running, moderate-speed running, and high-speed running), controlling the effects of match result, competition phase, and team and opponent strength. Results showed that the differences in the match running performance of UEFA and CONMEBOL players were trivial (ES between 0.04 and 0.14); players from AFC, CAF, and CONCACAF covered less total distance (ES between 0.26 and 0.54), spent less playing time, and covered less distance in jogging and low-speed running (ES between 0.20 and 0.53), whereas they spent more time walking (ES between 0.27 and 0.41) as compared with players from UEFA and CONMEBOL; top speed achieved, number of sprints made, and time spent and distance covered in the moderate- and high-speed running intensity zones by players from all confederations were similar (ES between 0.01 and 0.15), with an exception that high-speed-running distance covered by CONCACAF players was less than that by CAF players (2.0 ± 1.5 m/min vs. 2.3 ± 1.7 m/min, ES = 0.23, ±90% CL: ±0.21).
Background:
The mortality and disability rates of acute coronary syndrome (ACS) are quite high. Circular RNA (circRNA) is a competitive endogenous RNA (ceRNA) that plays an important role in the ...pathophysiology of ACS. Our goal is to screen circRNA-associated ceRNA networks for biomarker genes that are conducive to the diagnosis or exclusion of ACS, and better understand the pathology of the disease through the analysis of immune cells.
Materials and methods:
RNA expression profiles for circRNAs (GSE197137), miRNAs (GSE31568), and mRNAs (GSE95368) were obtained from the GEO database, and differentially expressed RNAs (DEcircRNAs, DEmiRNAs, and DEmRNAs) were identified. The circRNA-miRNA and miRNA-mRNA regulatory links were retrieved from the CircInteractome database and TargetScan databases, respectively. As a final step, a regulatory network has been designed for ceRNA. On the basis of the ceRNA network, hub mRNAs were verified by quantitative RT-PCR. Hub genes were validated using a third independent mRNA database GSE60993, and ROC curves were used to evaluate their diagnostic values. The correlation between hub genes and immune cells associated with ACS was then analyzed using single sample gene set enrichment analysis (ssGSEA).
Results:
A total of 17 DEcircRNAs, 229 DEmiRNAs, and 27 DEmRNAs were found, as well as 52 circRNA-miRNA pairings and 10 miRNA-mRNA pairings predicted. The ceRNA regulatory network (circRNA-miRNA-mRNA) was constructed, which included 2 circRNA (hsa_circ_0082319 and hsa_circ_0005654), 4 miRNA (hsa-miR-583, hsa-miR-661, hsa-miR-671-5p, hsa-miR-578), and 5 mRNA (
XPNPEP1, UCHL1, DBNL, GPC6,
and
RAD51
). The qRT-PCR analysis result showed that the
XPNPEP1, UCHL1, GPC6
and
RAD51
genes had a significantly decreased expression in ACS patients. Based on ROC curve analysis, we found that
XPNPEP1
has important significance in preventing ACS occurrence and excluding ACS diagnosis. ACS immune infiltration analysis revealed significant correlations between the other 3 hub genes (UCHL1, GPC6, RAD51) and the immune cells (Eosinophils, T folliculars, Type 2 T helper cells, and Imumature dendritic cells).
Conclusion:
Our study constructed a circRNA-related ceRNA network in ACS. The
XPNPEP1
gene could be a protective gene biomarker for ACS. The
UCHL1, GPC6 and RAD51
genes were significantly correlated with immune cells in ACS.
A biological treatment is the core process for removing organic pollutants from industrial wastewater. However, industrial wastewater often contains large amounts of toxic and harmful pollutants, ...which can inhibit the activity of microorganisms in a treatment system, precipitate the deterioration of effluent quality, and threaten water ecological security from time to time. In most of the existing anaerobic biological treatment processes, toxic effects on microorganisms are determined according to the amounts of end-products of the biochemical reactions, and the evaluation results are relatively lacking. When microorganisms contact toxic substances, changes in biological metabolic activity precede the accumulation of reaction products. As sensitive units, electroactive microorganisms can generate electrical signals, a change in which can directly reflect the toxicity level. The applications of electroactive microorganisms for the toxicity monitoring of wastewater are very promising. Further attention needs to be paid to considering the appropriate evaluation index, the influence of the environment on test results, mechanisms, and other aspects. Therefore, we reviewed the literature regarding the above aspects in order to provide a research foundation for the practical application of electroactive microorganisms in toxicant monitoring.
The axial-flux permanent magnet (AFPM) machines with yokeless and segmented armature (YASA) topology are suitable for in-wheel traction systems due to the high power density and efficiency. To ...guarantee the reliable operation of the YASA machines, an accurate thermal analysis should be undertaken in detail during the electrical machine design phase. The technical contribution of this paper is to establish a detailed thermal analysis model of the YASA machine by the lumped parameter thermal network (LPTN) method. Compared with the computational fluid dynamics (CFD) method and the finite element (FE) method, the LPTN method can obtain an accurate temperature distribution with low time consumption. Firstly, the LPTN model of each component of the YASA machine is constructed with technical details. Secondly, the losses of the YASA machine are obtained by the electromagnetic FE analysis. Then, the temperature distribution of the machine can be calculated by the LPTN model and loss information. Finally, a prototype of the YASA machine is manufactured and its temperature distribution under different operating conditions is tested by TT-K-30 thermocouple temperature sensors. The experimental data matches the LPTN results well.
Abstract Background Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) by traditional methods are a mix of atrial and ventricular CMs and many other non-cardiomyocyte ...cells. Retinoic acid (RA) plays an important role in regulation of the spatiotemporal development of the embryonic heart. Methods CMs were derived from hiPSC (hi-PCS-CM) using different concentrations of RA (Control without RA, LRA with 0.05μM and HRA with 0.1 μM) between day 3-6 of the differentiation process. Engineered heart tissues (EHTs) were generated by assembling hiPSC-CM at high cell density in a low collagen hydrogel. Results In the HRA group, hiPSC-CMs exhibited highest expression of contractile proteins MYH6, MYH7 and cTnT. The expression of TBX5, NKX2.5 and CORIN, which are marker genes for left ventricular CMs, was also the highest in the HRA group. In terms of EHT, the HRA group displayed the highest contraction force, the lowest beating frequency, and the highest sensitivity to hypoxia and isoprenaline, which means it was functionally more similar to the left ventricle. RNAsequencing revealed that the heightened contractility of EHT within the HRA group can be attributed to the promotion of augmented extracellular matrix strength by RA. Conclusion By interfering with the differentiation process of hiPSC with a specific concentration of RA at a specific time, we were able to successfully induce CMs and EHTs with a phenotype similar to that of the left ventricle or right ventricle.
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