The detection of frame deletion forgery is of great significance in the field of video forensics. Existing approaches, however, are not applicable to video sequences with variable motion strengths. ...In addition, the impact of interfering frames has not been considered in these approaches. Our research aims to develop a motion-adaptive forensic method as well as to eliminate interfering frames. Through a study of the statistical characteristics of the most common interfering frames such as relocated I-frames, we develop a new fluctuation feature based on frame motion residuals to identify frame deletion points (FDPs). The fluctuation feature is further enhanced by an intra-prediction elimination procedure so that it can be adapted to sequences with various motion levels. The enhanced feature is measured using a moving window detector to identify the location of a FDP. Finally, a postprocessing procedure is proposed to eliminate the minor interferences of sudden lighting change, focus vibration, and frame jitter. Our experimental results demonstrate that for videos with variable motion strengths and different interfering frames, the true positive rate of the algorithm can reach 90% when the false alarm rate is 0.3%. Our proposed method could provide a foundation for many practical applications of video forensics.
The September 2022 Ms 6.8 Luding earthquake broke more than 230 years of seismic calm on the Moxi fault, providing a unique opportunity to understand its seismogenic environment, rupture dynamics, ...and seismic hazard. Using teleseismic body waves, regional strong‐motion observations, GNSS, and InSAR data, we decipher the spatiotemporal rupture evolution of the mainshock. Combining the elastic dislocation model with surface creep, we find that the coseismic slip correlates closely with a locked patch with a loading rate of 9.7 mm/yr, but the creeping rate is insufficient to make up the shallow slip deficit. Notably, the Luding earthquake ruptured only ∼1/4 of the Moxi seismic gap, and it further increased the stress in the unruptured northern part. We thus argue that the Moxi fault has the potential for magnitude 7+ earthquakes in the near future, although geodetic prediction may overestimate the actual seismic moment released by the coseismic rupture.
Plain Language Summary
The Xianshuihe fault is an area of intense tectonic activity on the Tibetan Plateau and plays an important role in accommodating the postcollisional convergence of the Eurasian and Indian plates. However, the Moxi fault, at the southeastern end of the Xianshuihe fault, has remained seismically quiescent since the last Ms 7.75 event in 1786. In September 2022, a Ms 6.8 Luding earthquake struck the Moxi seismic gap, resulting in destructive landslide damage, with 93 people dead and 25 missing. Using seismic and geodetic recordings, we recover its space‐time rupture process in detail. Our model reveals an asymmetric bilateral rupture with dominant propagation and maximum slip to the SSE. It is important to note that the 2022 Luding earthquake ruptured only the southern Moxi seismic gap, leaving the northern part unruptured. Based on the departure time of the last major earthquake and the slip rate obtained from the elastic dislocation model, we estimate the unreleased seismic energy of the Moxi fault, indicating that it still has the potential for strong earthquakes. Besides, the Luding earthquake further increased its seismic hazard through stress transfer. Therefore, we should pay great attention to the kinematic state of the Moxi fault at this time.
Key Points
The kinematic evolution of the Luding earthquake is revealed by seismic and geodetic observations
The coseismic slip asperity from 3 to 20 km depth correlates closely with the preseismic surface creep and locked patch
The unruptured Moxi seismic gap still has the potential for magnitude 7+ earthquakes and deserves further attention
Lung adenocarcinoma (LUAD) is the predominant type of non-small lung cancer (NSCLC) with strong invasive ability and poor prognosis. The drug resistance related genes are potentially associated with ...prognosis of LUAD. Our research aimed to identify the drug resistance related genes and explore their potential prognostic value in LUAD patients. The data used in this study were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Firstly, we screened drug resistance related genes in LUAD by differential gene analysis, univariate Cox regression and drug sensitivity analyses. Subsequently, we constructed a risk score model using LASSO Cox regression analysis, and verified whether the risk score can predict the survival of LUAD patients independent of other factors. Moreover, we explored the immune infiltration of 22 immune cells between high-risk and low-risk patients. Totally 10 drug-resistance positively related genes (PLEK2, TFAP2A, KIF20A, S100P, GDF15, HSPB8, SASH1, WASF3, LAMA3 and TCN1) were identified in LUAD. The risk score model of LUAD constructed with these 10 genes could reliably predict the prognosis of LUAD patients. 18 pathways were significantly activated in high-risk group compared with low-risk group. In addition, the infiltration proportion of multiple immune cells was significantly different between high-risk and low-risk groups, and the proportion of M1 phagocytes was significantly higher in the high-risk group compared with the low-risk group. The drug resistance related genes (PLEK2, TFAP2A, KIF20A, S100P, GDF15, HSPB8, SASH1, WASF3, LAMA3 and TCN1) could predict the prognosis of LUAD patients. Clarifying the roles and mechanisms of these 10 genes in regulating drug resistance in LUAD will help to improve individualized clinical treatment protocols and predict patient sensitivity to treatment.
Almost all flowers of the tea plant (
) are white, which has caused few researchers to pay attention to anthocyanin accumulation and color changing in tea flowers. A new purple-leaf cultivar, Baitang ...purple tea (BTP) was discovered in the Baitang Mountains of Guangdong, whose flowers are naturally pink, and can provide an opportunity to understand anthocyanin metabolic networks and flower color development in tea flowers. In the present study, twelve anthocyanin components were identified in the pink tea flowers, namely cyanidin
-syringic acid, petunidin 3-
-glucoside, pelargonidin 3-
-beta-d-glucoside, which marks the first time these compounds have been found in the tea flowers. The presence of these anthocyanins seem most likely to be the reason for the pink coloration of the flowers. Twenty-one differentially expressed genes (DEGs) involved in anthocyanin pathway were identified using KEGG pathway functional enrichment, and ten of these DEG's screened using venn and KEGG functional enrichment analysis during five subsequent stages of flower development. By comparing DEGs and their expression levels across multiple flower development stages, we found that anthocyanin biosynthesis and accumulation in BTP flowers mainly occurred between the third and fourth stages (BTP3 to BTP4). Particularly, during the period of peak anthocyanin synthesis 17 structural genes were upregulated, and four structural genes were downregulated only. Ultimately, eight critical genes were identified using weighted gene co-expression network analysis (WGCNA), which were found to have direct impact on biosynthesis and accumulation of three flavonoid compounds, namely cyanidin 3-
-glucoside, petunidin 3-
-glucoside and epicatechin gallate. These results provide useful information about the molecular mechanisms of coloration in rare pink tea flower of anthocyanin-rich tea, enriching the gene resource and guiding further research on anthocyanin accumulation in purple tea.
Long-term visualization of the dynamic interactions between intracellular structures throughout the three-dimensional space of whole live cells is essential to better understand their functions, but ...this task remains challenging due to the limitations of existing three-dimensional fluorescence microscopy techniques, such as an insufficient axial resolution, low volumetric imaging rate and photobleaching. Here, we present the combination of a progressive deep-learning super-resolution strategy with a double-ring-modulated selective plane illumination microscopy design capable of visualizing the dynamics of intracellular structures in live cells for hours at an isotropic spatial resolution of roughly 100 nm in three dimensions at speeds up to roughly 17 Hz. Using this approach, we reveal the complex spatial relationships and interactions between endoplasmic reticulum (ER) and mitochondria throughout live cells, providing new insights into ER-mediated mitochondrial division. We also examined the motion of Drp1 oligomers involved in mitochondrial fission and revealed the dynamic interactions between Drp1 and mitochondria in three dimensions.
A variant of tea tree (
(L.)) with purple buds and leaves and pink flowers can be used as a unique ornamental plant. However, the mechanism of flower coloration remains unclear. To elucidate the ...molecular mechanism of coloration, as well as anthocyanin accumulation in white and pink tea flowers, metabolite profiling and transcriptome sequencing was analyzed in various tea flower developmental stages. Results of metabolomics analysis revealed that three specific anthocyanin substances could be identified, i.e., cyanidin
-syringic acid, petunidin 3-
-glucoside, and pelargonidin 3-
-β-d-glucoside, which only accumulated in pink tea flowers, and were not able to be detected in white flowers. RNA-seq and weighted gene co-expression network analysis revealed eight highly expressed structural genes involved in anthocyanin biosynthetic pathway, and particularly, different expression patterns of flavonol synthase and dihydroflavonol-4-reductase genes were observed. We deduced that the disequilibrium of expression levels in flavonol synthases and dihydroflavonol-4-reductases resulted in different levels of anthocyanin accumulation and coloration in white and pink tea flowers. Results of qRT-PCR performed for 9 key genes suggested that the expression profiles of differentially expressed genes were generally consistent with the results of high-throughput sequencing. These findings provide insight into anthocyanin accumulation and coloration mechanisms during tea flower development, which will contribute to the breeding of pink-flowered and anthocyanin-rich tea cultivars.
Background The continuous activation of transcription factors drives many diseases, including tumors, autoimmune disease, neurodegenerative disease, and male infertility. Thus, Blocking the ...transcriptional activity of these proteins may inhibit disease progression. In this study, we developed a new method to specifically inhibit the activity of the transcription factor STAT3. Methods Fusing the transcriptional inhibitory domain KRAB with STAT3 successfully blocked the transcription activity of STAT3 in cancer cells without affecting its function in the mitochondria and lysosomes. Results the expression of KRAB-STAT3 fusion protein inhibited the growth of tumor cells. Conclusions The KRAB-STAT3 fusion protein provides a novel approach for drug development for the treatment of cancer or autoimmune diseases. Keywords: KRAB, STAT3, Transcription, cancer, Autoimmune diseases, Neurodegenerative diseases, Male infertility
Common walnut (Juglans regia L.) is one of the top four most consumed nuts in the world due to its health benefits and pleasant taste. Despite its economic importance, the evolutionary history and ...genetic control of its adaptation and agronomic traits remain largely unexplored.
We report a comprehensive walnut genomic variation map based on whole-genome resequencing of 815 walnut accessions. Evolutionary analyses suggest that Chinese J. regia diverged from J. sigillata with extensive hybridizations after the split of the two species. In contrast to annual crops, the genetic diversity and heterozygous deleterious mutations of Chinese common walnut trees have continued to increase during the improvement process. Selective sweep analyses identify 902 genes uniquely selected in the improved common walnut compared to its progenitor population. Five major-effect loci are identified to be involved in walnut adaptations to temperature, precipitation, and altitude. Genome-wide association studies reveal 27 genomic loci responsible for 18 important agronomic traits, among which JrFAD2 and JrANR are the potentially major-effect causative genes controlling linoleic acid content and color of the endopleura of the nut, respectively.
The largest genomic resource for walnuts to date has been generated and explored in this study, unveiling their evolutionary history and cracking the genetic code for agronomic traits and environmental adaptation of this economically crucial crop tree.
Using rumor verification data from investor interactive platforms, we investigate the effect of stock market rumors on price efficiency. We find favorable rumors are positively correlated with stock ...price synchronicity, while unfavorable rumors are negatively correlated with stock price synchronicity. Both favorable and unfavorable rumors are positively correlated with stock mispricing levels, and stock price crash risk. Mechanism tests reveal that favorable rumors about industry leaders have industry spillover effects. The effect of rumors on mispricing levels and stock price crash risk are more pronounced when there are more retail investors. Further analysis shows stronger detrimental impacts of rumors on price efficiency for small-cap companies, companies with low information transparency and companies with low institutional ownership.
The current study aimed to elucidate the effects of Sevoflurane on neuronal autophagy and ischemic brain injury by regulating miR-7a/ATG7 axis. The rat model of middle cerebral artery occlusion ...(MCAO) was established by thread embolization. The expression pattern of microRNA-7a (miR-7a) and autophagy-related gene 7 (ATG7) was subsequently determined in Sevoflurane-treated MCAO rats with their relation and effects on neuronal autophagy and ischemic brain injury further analyzed. Bioinformatics analysis confirmed that miR-7a could target to inhibit ATG7 in ischemic brain injury samples. Sevoflurane could alleviate ischemic brain injury in rats by reducing the level of neuronal autophagy-related factors. The expression of miR-7a was up-regulated and ATG7 was down-regulated in the brain tissues of MCAO rats after Sevoflurane treatment. ATG7 was found to induce neuronal autophagy during autophagy in the brain tissues of MCAO rats. In summary, Sevoflurane exerts protective effects on ischemic brain injury via inhibiting autophagy of neurons and microglia through the miR-7a-mediated downregulation of ATG7.
•Sevoflurane alleviated ischemic brain injury in rats by reducing autophagy.•Sevoflurane upregulated miR-7a to inhibit ischemic brain injury.•miR-7a negatively regulates the expression of ATG7.•ATG7 reversed the protective effect of Sevoflurane on ischemic brain injury.•The study highlights a new preventive option for ischemic brain injury.
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