Cross-database micro-expression recognition (CDMER) is one of recently emerging and interesting problem in micro-expression analysis. CDMER is more challenging than the conventional micro-expression ...recognition (MER), because the training and testing samples in CDMER come from different micro-expression databases, resulting in inconsistency of the feature distributions between the training and testing sets. In this paper, we contribute to this topic from three aspects. First, we establish a CDMER experimental evaluation protocol aiming to allow the researchers to conveniently work on this topic and evaluate their proposed methods under the same standard. Second, we conduct benchmark experiments by using NINE state-of-the-art domain adaptation (DA) methods and SIX popular spatiotemporal descriptors for investigating CDMER problem from two different perspectives. Third, we propose a novel DA method called region selective transfer regression (RSTR) to deal with the CDMER task. The overall superior performance of RSTR over the state-of-the-art DA methods demonstrates that taking into consideration the facial local region information used in RSTR contributes to developing effective DA methods for dealing with CDMER problem.
Sphingosine kinase 2 (SPK2) and autophagy are both involved in brain preconditioning, but whether preconditioning-induced SPK2 up-regulation and autophagy activation are linked mechanistically ...remains to be elucidated. In this study, we used in vitro and in vivo models to explore the role of SPK2-mediated autophagy in isoflurane and hypoxic preconditioning. In primary mouse cortical neurons, both isoflurane and hypoxic preconditioning induced autophagy. Isoflurane and hypoxic preconditioning protected against subsequent oxygen glucose deprivation or glutamate injury, whereas pretreatment with autophagy inhibitors (3-methyladenine or KU55933) abolished preconditioning-induced tolerance. Pretreatment with SPK2 inhibitors (ABC294640 and SKI-II) or SPK2 knockdown prevented preconditioning-induced autophagy. Isoflurane also induced autophagy in mouse in vivo as shown by Western blots for LC3 and p62, LC3 immunostaining, and electron microscopy. Isoflurane-induced autophagy in mice lacking the SPK1 isoform (SPK1−/−), but not in SPK2−/− mice. Sphingosine 1-phosphate and the sphingosine 1-phosphate receptor agonist FTY720 did not protect against oxygen glucose deprivation in cultured neurons and did not alter the expression of LC3 and p62, suggesting that SPK2-mediated autophagy and protections are not S1P-dependent. Beclin 1 knockdown abolished preconditioning-induced autophagy, and SPK2 inhibitors abolished isoflurane-induced disruption of the Beclin 1/Bcl-2 association. These results strongly indicate that autophagy is involved in isoflurane preconditioning both in vivo and in vitro and that SPK2 contributes to preconditioning-induced autophagy, possibly by disrupting the Beclin 1/Bcl-2 interaction.
Background: Preconditioning provides insights into endogenous mechanisms that could be used to protect brain from injury.
Results: Preconditioning stimuli up-regulate sphingosine kinase 2, leading to autophagy.
Conclusion: Sphingosine kinase 2 mediates autophagy and preconditioning, possibly by disrupting Beclin 1/Bcl-2 interaction.
Significance: The discovery of new signaling independent of SPK2 catalytic activity provides medicinal chemists with novel “druggable” targets important for neuroprotection.
This study aims to investigate the effect of microRNA‐129 (miR‐129) on proliferation and apoptosis of hippocampal neurons in epilepsy rats by targeting c‐Fos via the MAPK signaling pathway. Thirty ...rats were equally classified into a model group (successfully established as chronic epilepsy models) and a normal group. Expression of miR‐129, c‐Fos, bax, and MAPK was detected by RT‐qPCR and Western blotting. Hippocampal neurons were assigned into normal, blank, negative control (NC), miR‐129 mimic, miR‐129 inhibitor, siRNA‐c‐Fos, miR‐129 inhibitor+siRNA‐c‐Fos groups. The targeting relationship between miR‐129 and c‐Fos was predicted and verified by bioinformatics websites and dual‐luciferase reporter gene assay. Cell proliferation after transfection was measured by MTT assay, and cell cycle and apoptosis by flow cytometry. c‐Fos is a potential target gene of miR‐129. Compared with the normal group, the other six groups showed a decreased miR‐129 expression; increased expression of expression of c‐Fos, Bax, and MAPK; decreased proliferation; accelerated apoptosis; more cells arrested in the G1 phase; and fewer cells arrested in the S phase. Compared with the blank and NC groups, the miR‐129 mimic group and the siRNA‐c‐Fos group showed decreased expression of c‐Fos, Bax, and MAPK, increased cells proliferation, and decreased cell apoptosis, fewer cells arrested in the G1 phase and more cells arrested in the S phase. However, the miR‐129 inhibitor groups showed reverse consequences. This study suggests that miR‐129 could inhibit the occurrence and development of epilepsy by repressing c‐Fos expression through inhibiting the MAPK signaling pathway.
This study suggests that miR‐129 could inhibit the occurrence and development of epilepsy by repressing c‐Fos expression through the inhibition of the MAPK signaling pathway.
Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, ...sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains.
Sinopodophyllum hexandrum
is a perennial anti-cancer medicinal plant as unique phytochemical composition podophyllotoxin, and it has special effects on the treatments of pneumonic, cervical and ...testicular cancers. Besides the podophyllotoxin, phenolic substances play a key role in the clinical practice. However, few reports were available in terms of the phenolic compositions and antioxidant activity. In this work, main phenolic compounds were quantified by RP-HPLC in seven organs from
S. hexandrum
. Simultaneously, the sodium borohydride/chloranil-based (SBC) method and the Folin-Ciocalteau colorimetric method were used to determine total flavonoids and total phenols contents, respectively. The antioxidant activity of the different organs was further assessed by three methods (DPPH method, ABTS method and FRAP method). Phenolic compositions/total flavonoids contents/total phenols contents/antioxidant activity was observed to have significant differences among different organs (
P
<0.05), but have a consistent changing rule viz. rhizome>root>fruit>flower>leaf>stem>petiole. Furthermore, a correlation analysis was employed and indicated that a positive correlation existed between phenolic compositions contents and antioxidant activity. Obviously, rhizome had high phenolic compositions contents and strong antioxidant activity with the low DPPH
IC50
value of 23.52 μg/mL, high ABTS value of 1137.82 μmol equiv. Trolox/g and high FRAP value of 685.76 μmol equiv. Trolox/g. Therefore, rhizome is recommended as a preponderant medicinal part, and root is proposed as an alternative raw material resource for natural antioxidant agents in functional food, medicine and chemicals. This study can provide a new insight into the utilization extension of
S. hexandrum
resources.
•Associations of phthalates exposure with thyroid cancer and nodule were explored.•Urinary DEHP metabolites associated with increased risks of thyroid cancer and nodule.•Urinary MBP was inversely ...associated with risks of thyroid cancer and nodule.•Some of the associations were sex-specific.
Phthalates have been reported to affect the function and growth of thyroid. However, there is little data on the effect of phthalates on thyroid oncogenesis. Here we explored the associations between phthalates exposure and the risks of thyroid cancer and benign nodule. We sex-matched 144 thyroid cancer, 138 benign nodule patients and 144 healthy adults from Wuhan, China. Eight phthalate metabolites in spot urine samples were quantified using high-performance liquid chromatography and tandem mass spectrometry. The associations of creatinine-corrected urinary phthalate metabolites with the risks of thyroid cancer and benign nodule were assessed using multivariable logistic regression models. We found that urinary monomethyl phthalate (MMP), mono(2-ethyl-5hydroxyhexyl) phthalate (MEHHP) and mono(2-ethylhexyl) phthalate (MEHP) associated with increased risks of thyroid cancer and nodule, with adjusted odds ratios (ORs) ranging from 1.74 to 4.78 comparing the extreme tertiles, and urinary monobutyl phthalate (MBP) was associated with decreased risks of thyroid cancer and benign nodule (all P for trends < 0.05). Male-specific positive associations of urinary monoethyl phthalate (MEP) with thyroid cancer and nodule as well as urinary mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) with thyroid cancer were also observed. Our results suggest that exposure to certain phthalates may contribute to increased risks of thyroid cancer and benign nodule.
Soil is one of the largest reservoirs and re-emission sources of mercury (Hg) on Earth’s surface. The accumulation and remobilization of Hg during pedogenesis affect how Hg is transported from ...terrestrial to aquatic environment and biota, but these processes are poorly understood. Here we present Hg concentration and isotope ratios of soil and bedrock samples from a latosol profile formed through intense weathering of Cenozoic basalts in Zhanjiang, Guangdong Province, China, to trace the sources, transport and transformation processes of Hg during pedogenesis, and to gain insights on the potential impact of tropical soils on global Hg cycling. The entire soil profile shows negative δ202Hg (−2.97‰ ± 0.08‰ to −2.54‰ ± 0.08‰) and negative Δ199Hg (−0.68‰ ± 0.04‰ to −0.43‰ ± 0.04‰), with a gradual downward positive shift of Δ199Hg below 35 cm. An isotopic mixing model based on Δ199Hg suggests a dominant Hg input from atmospheric Hg (HgAtm) and limited contribution (<36%) of geogenic Hg (HgGeo) from the bedrock. The strongly negative Δ199Hg throughout the latosol profile suggests that HgAtm was likely subject to substantial photoreduction on soil surface and migrated downward after incorporation into soils. Moreover, there is a distinct positive shift of δ202Hg alongside a rapid decrease of HgGeo from the bedrock to the weakly weathered basalt, indicating a substantial loss of HgGeo with the preferential release of lighter isotopes during the initial weathering, likely due to the dissolution of primary minerals. In addition, the δ202Hg shows a negative correlation with soil pH at some horizons, attributable to the isotopic fractionation during Hg(II) speciation change in soil solution and the selective complexation/adsorption of different Hg(II) species onto mineral surfaces, which are affected by surface charge properties and thus soil pH. Overall, our results provide direct evidence for the accumulation and downward migration of HgAtm in tropical soils during pedogenesis, as well as the loss of HgGeo during the weathering of bedrock, which may serve as an underappreciated source of Hg in tropical regions and have a potential impact on the Hg isotope signatures in aquatic environments.
We propose a method to search for stellar-mass black hole (BH) candidates with giant companions from spectroscopic observations. Based on the stellar spectra of Large sky Area Multi-Object fiber ...Spectroscopic Telescope (LAMOST) Data Release 6, we obtain a sample of seven giants in binaries with large radial velocity variation ΔVR > 80 km s−1. With the effective temperature, surface gravity, and metallicity provided by LAMOST, and the parallax given by Gaia, we can estimate the mass and radius of the giant, and therefore evaluate the possible mass of the optically invisible star in the binary. We show that the sources in our sample are potential BH candidates, and are worthy of dynamical measurement by further spectroscopic observations. Our method may be particularly valid for the selection of BH candidates in binaries with unknown orbital periods.
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