Background and Objective
Periodontitis is a multifactorial disease that can lead to the progressive destruction of dental support tissue. However, the detailed mechanisms and specific biomarkers ...involved in periodontitis remain to be further studied. Recently, long non‐coding RNAs (lncRNAs) have been found to play a more important role than other types of RNAs. In our study, we analysed the expression of lncRNAs in periodontitis by analysing GSE16134.
Material and Methods
We identified highly correlated genes by analysing GSE16134 with weighted gene co‐expression network analysis (WGCNA) and identified 50 hub lncRNAs that were dysregulated. Then, we used the Linear Models for Microarray Data (Limma) package to identify the hub lncRNAs that were differentially expressed (DElncRNAs). The ceRNA co‐expression network data were obtained from several sites, including miRcode, and were used to assess the potential WGCNA function of hub DElncRNAs in periodontitis. Besides, we divided the samples into LBX2‐AS1 high and low expression group by the expression level of LBX2‐AS1 and calculated DEG by Limma package. Furthermore, we performed GO function, KEGG pathway and GSEA enrichment of DEGs.
Results
In the analysis, we identified 50 hub lncRNAs that may play important roles in periodontitis. Then, we used the Limma package to identify 3 hub DElncRNAs (LINC00687, LBX2‐AS1 and LINC01566). We elucidated the potential function of the hub DElncRNA LBX2‐AS1 in periodontitis by constructing a co‐expression network of lncRNA‐miRNA‐mRNA interactions. Totally, 573 DEGs (354 up‐ and 219 downregulated) in periodontitis samples were identified. DEGs were enriched in different GO terms and pathways, such as neutrophil degranulation, neutrophil activation, neutrophil activation involved in immune response, neutrophil‐mediated immunity, antigen processing and presentation, JAK‐STAT signalling pathway, natural killer cell‐mediated cytotoxicity, EGFR tyrosine kinase inhibitor resistance, phosphatidylinositol signalling system and Vascular Endothelial Growth Factor (VEGF) signalling pathway.
Conclusion
In our study, we found that 3 hub DElncRNAs (LINC00687, LBX2‐AS1 and LINC01566) may be involved in the pathogenesis of periodontitis based on WGCNA and Limma analysis. Our study aimed to elucidate the mechanisms involved in periodontitis at the genetic and epigenetic levels by constructing a ceRNA network associated with lncRNA. Besides, identification DEGs of differential LBX2‐AS1 and functional annotation showed that LBX2‐AS1 might be associated with periodontitis.
Chemotherapy‐induced peripheral neuropathy (CIPN) is among the most disabling and frustrating problems for cancer survivors. The neurotoxicity caused by cisplatin varies greatly among patients, and ...few predictors of appearance, duration of symptoms, susceptibility, or severity are available. A deeper understanding of the mechanisms underlying individual differences in status, severity, or sensitivity in response to cisplatin treatment is therefore required. By analyzing the GSE64174 gene expression profile and constructing a weighted gene co‐expression network analysis (WGCNA) network, we screened gene modules and hub genes related to CIPN status, severity and sensitivity. We first identified the transcriptome profile of mouse dorsal root ganglion (DRG) samples and transformed their genes to human DRG counterparts. We then constructed WGCNA gene modules via optimal soft‐threshold power‐identification and module‐preservation analysis. Comprehensive analysis and identification of module hub genes were performed via functional‐enrichment analysis and significant common hub genes were identified, including “Cytoscape_cytoHubba,” “Cytoscape_MCODE,” and “Metascape_MCODE.” Brown, green, and blue modules were selected to represent CIPN sensitivity, status, and severity, respectively, via trait‐module correlational analysis. Additionally, functional enrichment analysis results indicated that these three modules were associated with some crucial biological functions, such as neutrophil migration, chemokine‐mediated signaling pathway, and PI3K‐Akt signaling pathway. We then identified seven common hub genes via three methods, including CXCL10, CCL21, CCR2, CXCR4, TLR4, NPY1R, and GALR2, related to CIPN status, severity and sensitivity. Our results provide possible targets and mechanism insights into the development and progress of CIPN, which can guide further transformation and pre‐clinical research.
From July 2019 to January 2020, 70 women with AMA who had chosen selective caesarean section in the Third Affiliated Hospital of Nanchang University and 65 non-pregnant women who had undergone ...selective surgery in our hospital were both included in the study (The inclusion and exclusion criteria are detailed in the supplementary materials). ...it can be concluded that CSA cannot be predicted by a single variable nor can the size of CSA be judged by a single index, such as the length of gestation week or fasting time. ...this study is a single center study, the number of cases included is limited, and multi center, large sample data are needed to verify this result. ...future research should indicate whether the new fasting guidelines are also applicable to women with AMA.Funding No external funding.Declaration of Competing Interest The authors have no interests to disclose with the matter.Appendix A Supplementary data Supplementary dataImage 1Appendix A Supplementary data Supplementary data to this article can be found online at https://doi.org/10.1016/j.jclinane.2021.110270.
Although ultrasound-guided transversus abdominis plane block (TAPB) is widely used in multimodal analgesia after cesarean delivery (CD), the complications of TAPB during analgesia after CD have ...rarely been reported.
A total of 84 cases of CD were randomly assigned to either a ropivacaine group (R group) or ropivacaine + dexamethasone group (RD group) in this double-blind trial. The pain site and pain degree at rest and during activity at 2 h, 6 h, 10 h, 12 h, 14 h, 16 h, 20 h, and 24 h after maternal surgery were recorded. The consumption of opioids at 24h, postoperative nausea, vomiting, exhaustion, and other adverse reactions were recorded.
A total of 80 patients were included in the analysis of results. A total of 19 patients developed ISP, 14 in the R group and 5 in the RD group. The incidence of ISP in the R and RD groups was 35% and 12.5%, respectively. The results described above showed that combining dexamethasone with ropivacaine reduced the incidence of ISP, and the difference was statistically significant (P<0.05). Two groups of women with positive ISP had higher values of opioid consumption than women with negative ISP, but the difference was not significant.
Dexamethasone as an adjuvant for ropivacaine can effectively relieve the ISP of ultrasound-guided TAPB after CD, and can enhance the analgesic effect of ropivacaine.
This study aimed to explore the genetic and epigenetic similarities between chronic obstructive pulmonary disease (COPD) and lung adenocarcinoma (LUAD).
We mainly used Weighted correlation network ...analysis, protein–protein interaction network and pivot analysis to identify hub modules, bridge regulators, bridge genes and hub-driving genes in both diseases and carried out verifying using external datasets.
We identified eight bridge regulators, 19 key molecules in the COPD model and ten key molecules in the LUAD model. Moreover, we validated that CDC5L could be a reliable biomarker in COPD and may regulate cell proliferation and metastasis in LUAD via promoter methylation.
Our results might form a theoretical foundation for future study at an epigenetic level.
Bilayer and multiple layers of two-dimensional materials exhibit sliding ferroelectricity due to the asymmetric interlayer orbital hybridization. The spin degree of freedom can be used to tune the ...interlayer orbital hybridization and hence the interlayer charge transfer. In this work, we utilize the bilayer of magnetic two-dimensional MnNiP2 S6 to demonstrate the idea. First-principles calculations confirm that the out-of-plane polarization depends on the magnetic configuration. A supersuperexchange path across the van der Waals gap with all the bond angles close to 180° is responsible for the interlayer exchange interaction and the spin-dependent interlayer charge transfer. This work provides a promising route for constructing a multiferroic two-dimensional system by stacking nonpolar magnetic monolayers.
Timely detection of modifiable risk factors for postoperative pulmonary complications (PPCs) could inform ventilation strategies that attenuate lung injury. We sought to develop, validate, and ...internally test machine learning models that use intraoperative respiratory features to predict PPCs.
We analysed perioperative data from a cohort comprising patients aged 65 yr and older at an academic medical centre from 2019 to 2023. Two linear and four nonlinear learning models were developed and compared with the current gold-standard risk assessment tool ARISCAT (Assess Respiratory Risk in Surgical Patients in Catalonia Tool). The Shapley additive explanation of artificial intelligence was utilised to interpret feature importance and interactions.
Perioperative data were obtained from 10 284 patients who underwent 10 484 operations (mean age range 71 65–98 yr; 42% female). An optimised XGBoost model that used preoperative variables and intraoperative respiratory variables had area under the receiver operating characteristic curves (AUROCs) of 0.878 (0.866–0.891) and 0.881 (0.879–0.883) in the validation and prospective cohorts, respectively. These models outperformed ARISCAT (AUROC: 0.496–0.533). The intraoperative dynamic features of respiratory dynamic system compliance, mechanical power, and driving pressure were identified as key modifiable contributors to PPCs. A simplified model based on XGBoost including 20 variables generated an AUROC of 0.864 (0.852–0.875) in an internal testing cohort. This has been developed into a web-based tool for further external validation (https://aorm.wchscu.cn/).
These findings suggest that real-time identification of surgical patients' risk of postoperative pulmonary complications could help personalise intraoperative ventilatory strategies and reduce postoperative pulmonary complications.
Little is known about the recent use of neuromuscular blocking agents (NMBAs) and monitoring in China. This paper presents the results of a nationwide survey conducted to obtain information regarding ...the current management of NMBAs in China.
A questionnaire was sent to Chinese anesthesiologists inviting them to participate in the study. The questionnaire was available through the
website, and the link was sent to 1,488 anesthesiologists using the
mini app.
The web-based survey consisted of 28 questions, and data were collected using an online tool. Between May 19, 2021 and June 16, 2021, 637 responses were collected (response rate = 42.8%). Only 10.2% of anesthesiologists reported using neuromuscular function monitors, and 6.59% of respondents reported that they had the relevant monitors in the operating room.
Although PORC is a potential safety issue, the frequency of using reversal agents and monitors remains extremely low in China. Surveys such as this are important to understand the use and application customs of NMBAs in China.
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