When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres ...cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exogenous collagen I. Collagen increased the interaction of the bacilli with A549 type II pneumocytes or U937 macrophages, suggesting that BCG is able to recruit collagen to facilitate its attachment to host cells. Using an affinity chromatography approach, we have isolated a BCG collagen-binding protein corresponding to the previously described mycobacterial laminin-binding histone-like protein (LBP/Hlp), a highly conserved protein associated with the mycobacterial cell wall. Moreover, Mycobacterium leprae LBP/Hlp, a well-characterized adhesin, was also able to bind collagen I. Finally, using recombinant fragments of M. leprae LBP/Hlp, we mapped the collagen-binding activity within the C-terminal domain of the adhesin. Since this protein was already shown to be involved in the recognition of laminin and heparan sulphate-containing proteoglycans, the present observations reinforce the adhesive activities of LBP/Hlp, which can be therefore considered as a multifaceted mycobacterial adhesin, playing an important role in both leprosy and tuberculosis pathogenesis.
RESUMO: Essa pesquisa parte do pressuposto que a Assistência Técnica e a Extensão Rural nos últimos anos tem feito a diferença na vida do agricultor rural familiar, por aproximar esse público dos ...conhecimentos técnicos, antes desconhecidos, e por proporcionar uma maior facilidade no acesso às políticas públicas existentes. O objetivo principal é diagnosticar os impactos na vida do produtor rural familiar beneficiado com o serviço de Ater dentro da atividade da ovinocultura. Esta pesquisa foi desenvolvida a partir de um simplificado referencial teórico sobre temas que justificam a ação da Ater no Projeto Dom Helder Câmara que está sendo executado no município de Santana do Ipanema, sertão de Alagoas. Embora o projeto ainda esteja em desenvolvimento, dada a prorrogação de sua vigência, é possível afirmar que houve melhoria nas condições de trabalho para os beneficiados com o projeto.
PALAVRAS-CHAVE: Assistência Técnica. Extensão Rural. Ovinocultura.
This research is based on the assumption that Technical Assistance and Rural Extension in recent years has made a difference in the life of the family rural farmer, by bringing this public closer to ...previously unknown technical knowledge, and by providing easier access to existing public policies. . The main objective is to diagnose the impacts on the life of the family farmers who benefit from the ATER service within the sheep farming activity. This research was developed from a simplified theoretical framework on themes that justify Ater's action in the Dom Helder Câmara Project that is being carried out in the municipality of Santana do Ipanema,in the hinterland of Alagoas. Although the project is still under development, given the extension of its duration, it is possible to state that there was an improvement in working conditions for those benefiting from the project.
...the higher prevalence of tympanic foramen found in the Northeast among other studies carried out in Brazil can be attributed to specific dietary habits in the region that could induce the later ...foramen occlusion. ...the existence of this foramen constitutes a route for the spread of infections, which can occur both from temporo-mandibular joint to external acoustic meatus and vice versa (Silva and Collins, 2012; Tozoglu et al., 2012). ...it is important to observe the presence of TF before performing orthognathic procedures on the temporo-mandibular joint. Laterality was recorded and the longitudinal and transverse diameter of the TF were measured with the aid of a digital caliper with an accuracy of 0.01 mm. Data analysis was performed using SPSS statistical software (Version 20.0, SPSS Inc., Chicago, IL). The Northeast has a food with dense foods, which are offered in the first years of the child's life. ...the tympanic part of the temporal bone, which continues to grow even after birth, is influenced by the pressure caused by chewing and swallowing (Akcam et al., 2011; Lee and Park, 2018) and, therefore, TF occlusion tends to occur later in life (Afghari et al., 2016).
É normal que educandos apresentem dificuldade na assimilação do conhecimento teórico da entomologia, já que exige alto grau de abstração. As atividades de campo são métodos utilizados para a redução ...deste impedimento, norteando uma aprendizagem significativa e amplificando o trabalho grupal. Sendo assim, este trabalho objetivou desenvolver a prática de coleta e catalogação de insetos para a confecção de coleções entomológicas nas Escolas Estaduais de Ensino Profissionalizante dos municípios de Guaiúba e Viçosa do Ceará. Para o desenvolvimento desse trabalho, os alunos coletaram insetos nas escolas e suas periferias, os exemplares capturados eram catalogados e inseridos em caixas entomológicas. Na execução das atividades pôde-se observar um grande comprometimento e interesse dos educandos, principalmente por se tratar de uma atividade diferenciada (fora da sala de aula); também, nas diversas fases da atividade, foi observado nitidamente o intercâmbio de conhecimentos, configurando-se assim, uma aprendizagem cooperativa, constatada por observações in loco. Ao final da disciplina foi constatado que nenhum educando necessitou de processo de intervenção educacional, uma vez que a análise das avaliações escritas e não escritas apresentou uma real assimilação do conhecimento. Nesse sentido, a confecção de coleção entomológica mostrou-se um recurso significativamente eficiente no ensino da disciplina de Entomologia Agrícola e Combate a Pragas e Doenças.
Introdução: A presença da disfagia no ambiente hospitalar aumenta os custos de cuidado com a saúde e leva a maior tempo de internação hospitalar sobrecarregando, portanto, o sistema de saúde. ...Pacientes internados, por diferentes doenças de base, podem apresentar risco para disfagia com necessidade de intervenção fonoaudiológica. Objetivo: Rastrear o risco de disfagia em pacientes internados em um Hospital Universitário. Métodos: Estudo do tipo transversal descritivo realizado no período de março de 2016 a setembro de 2018 em um Hospital Universitário. Os dados foram coletados por meio de análise de prontuário e aplicação do instrumento de rastreio - Eating Assessment Tool (EAT-10). Os pacientes que pontuaram 3 ou mais foram classificados com risco de disfagia. Para análise estatística foi considerado o nível de 5% de significância. Resultados: O risco de disfagia foi identificado em 191 (7,8%) pacientes. Houve prevalência do sexo masculino, idade superior a 60 anos, e a doença de base prevalente foi cardiovascular. Observou-se que a Disfonia (4,2%), refluxo gastroesofágico (3,8%), uso de traqueostomia (1,1%) e via alternativa de alimentação (3,1%) foram fatores associados ao risco de disfagia. Conclusão: Observou-se risco de disfagia nos pacientes internados principalmente nos pacientes do sexo masculino, idosos e cardiopatas. Disfonia, refluxo gastroesofágico, uso de traquestomia e via alternativa de alimentação foram fatores associados ao risco de disfagia.
When grown in the presence of exogenous collagen I, Mycobacterium bovis
BCG was shown to form clumps. Scanning electron microscopy
examination of these clumps revealed the presence of collagen fibres
...cross-linking the bacilli. Since collagen is a major constituent of the
eukaryotic extracellular matrices, we assayed BCG cytoadherence in the
presence of exogenous collagen I. Collagen increased the interaction of
the bacilli with A549 type II pneumocytes or U937 macrophages,
suggesting that BCG is able to recruit collagen to facilitate its
attachment to host cells. Using an affinity chromatography approach, we
have isolated a BCG collagen-binding protein corresponding to the
previously described mycobacterial laminin-binding histone-like protein
(LBP/Hlp), a highly conserved protein associated with the mycobacterial
cell wall. Moreover, Mycobacterium leprae LBP/Hlp, a
well-characterized adhesin, was also able to bind collagen I. Finally,
using recombinant fragments of M. leprae LBP/Hlp, we mapped the
collagen-binding activity within the C-terminal domain of the adhesin.
Since this protein was already shown to be involved in the recognition
of laminin and heparan sulphate-containing proteoglycans, the present
observations reinforce the adhesive activities of LBP/Hlp, which can be
therefore considered as a multifaceted mycobacterial adhesin, playing
an important role in both leprosy and tuberculosis pathogenesis.
Patients hospitalised with COVID-19 are at risk for thrombotic events after discharge; the role of extended thromboprophylaxis in this population is unknown.
In this open-label, multicentre, ...randomised trial conducted at 14 centres in Brazil, patients hospitalised with COVID-19 at increased risk for venous thromboembolism (International Medical Prevention Registry on Venous Thromboembolism IMPROVE venous thromboembolism VTE score of ≥4 or 2–3 with a D-dimer >500 ng/mL) were randomly assigned (1:1) to receive, at hospital discharge, rivaroxaban 10 mg/day or no anticoagulation for 35 days. The primary efficacy outcome in an intention-to-treat analysis was a composite of symptomatic or fatal venous thromboembolism, asymptomatic venous thromboembolism on bilateral lower-limb venous ultrasound and CT pulmonary angiogram, symptomatic arterial thromboembolism, and cardiovascular death at day 35. Adjudication was blinded. The primary safety outcome was major bleeding. The primary and safety analyses were carried out in the intention-to-treat population. This trial is registered at ClinicalTrials.gov, NCT04662684.
From Oct 8, 2020, to June 29, 2021, 997 patients were screened. Of these patients, 677 did not meet eligibility criteria; the remaining 320 patients were enrolled and randomly assigned to receive rivaroxaban (n=160 50%) or no anticoagulation (n=160 50%). All patients received thromboprophylaxis with standard doses of heparin during hospitalisation. 165 (52%) patients were in the intensive care unit while hospitalised. 197 (62%) patients had an IMPROVE score of 2–3 and elevated D-dimer levels and 121 (38%) had a score of 4 or more. Two patients (one in each group) were lost to follow-up due to withdrawal of consent and not included in the intention-to-treat primary analysis. The primary efficacy outcome occurred in five (3%) of 159 patients assigned to rivaroxaban and 15 (9%) of 159 patients assigned to no anticoagulation (relative risk 0·33, 95% CI 0·12–0·90; p=0·0293). No major bleeding occurred in either study group. Allergic reactions occurred in two (1%) patients in the rivaroxaban group.
In patients at high risk discharged after hospitalisation due to COVID-19, thromboprophylaxis with rivaroxaban 10 mg/day for 35 days improved clinical outcomes compared with no extended thromboprophylaxis.
Bayer.
When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres ...cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exogenous collagen I. Collagen increased the interaction of the bacilli with A549 type II pneumocytes or U937 macrophages, suggesting that BCG is able to recruit collagen to facilitate its attachment to host cells. Using an affinity chromatography approach, we have isolated a BCG collagen-binding protein corresponding to the previously described mycobacterial laminin-binding histone-like protein (LBP/Hlp), a highly conserved protein associated with the mycobacterial cell wall. Moreover, Mycobacterium leprae LBP/Hlp, a well-characterized adhesin, was also able to bind collagen I. Finally, using recombinant fragments of M. leprae LBP/Hlp, we mapped the collagen-binding activity within the C-terminal domain of the adhesin. Since this protein was already shown to be involved in the recognition of laminin and heparan sulphate-containing proteoglycans, the present observations reinforce the adhesive activities of LBP/Hlp, which can be therefore considered as a multifaceted mycobacterial adhesin, playing an important role in both leprosy and tuberculosis pathogenesis.
When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres ...cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exogenous collagen I. Collagen increased the interaction of the bacilli with A549 type II pneumocytes or U937 macrophages, suggesting that BCG is able to recruit collagen to facilitate its attachment to host cells. Using an affinity chromatography approach, we have isolated a BCG collagen-binding protein corresponding to the previously described mycobacterial laminin-binding histone-like protein (LBP/Hlp), a highly conserved protein associated with the mycobacterial cell wall. Moreover, Mycobacterium leprae LBP/Hlp, a well-characterized adhesin, was also able to bind collagen I. Finally, using recombinant fragments of M. leprae LBP/Hlp, we mapped the collagen-binding activity within the C-terminal domain of the adhesin. Since this protein was already shown to be involved in the recognition of laminin and heparan sulphate-containing proteoglycans, the present observations reinforce the adhesive activities of LBP/Hlp, which can be therefore considered as a multifaceted mycobacterial adhesin, playing an important role in both leprosy and tuberculosis pathogenesis.
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