The aim of this paper is to investigate whether and how multiple-criteria decision analysis, based on the analytic hierarchy process (AHP) approach, may support the participatory process of the ...public in the whole transportation planning process, especially in strategic planning and at the initial stages during which planning options are drawn up and the public are rarely involved. The AHP makes it possible to consider the multiple objectives of decision makers and allows public engagement to be deliberative, participatory, dynamic and flexible, which is independent of planning options. The method was specified and calibrated starting from a specific stated preferences survey, and its parameters were calibrated with respect to two scenarios: without any transport options and with real transport options. Different criteria (accessibility, travel safety, comfort, environment, landscape), subcriteria and corresponding indicators (qualitative, quantitative and dichotomous) were considered, and reciprocal weights were calibrated. Finally, a real planning scenario was implemented. Calibration results gave interesting insights into the public desires and expectations, made it possible to rank the different chosen criteria and sub-criteria and to understand the biases between preferences stated with or without transport options. The method can be easily updated and can be easily transferred to any case study.
•AHP is a flexible and powerful tool for understanding and quantifying Public preferences.•Accessibility, safety and environmental proved to be the most significant criteria.•Waiting time and monetary cost are the most significant accessibility sub-criteria.•The Public puts great importance on the infrastructural component of travel safety.•The Public prefers low congestion and reliable services to ATIS services.
Even if the first published article on a porous silicon (PSi)-based biosensor dates back to more than twenty years ago, this technology still attracts great attention from many research groups around ...the world. In this brief review, the pros and cons of porous silicon-based optical biosensors will be highlighted on the basis of some recent results and published papers on this subject. The aim of the paper is to give a straightforward introduction to PhD students and young researchers on this subject, which is particularly full of educative content, since it is highly multidisciplinary. Fabrication of PSi-based optical biosensors requires competencies related to many different scientific topics ranging from material science, physics and optics to healthcare and environmental monitoring through surface chemistry and more.
We describe an innovative experimental and computational approach to control the expression of a protein in a population of yeast cells. We designed a simple control algorithm to automatically ...regulate the administration of inducer molecules to the cells by comparing the actual protein expression level in the cell population with the desired expression level. We then built an automated platform based on a microfluidic device, a time-lapse microscopy apparatus, and a set of motorized syringes, all controlled by a computer. We tested the platform to force yeast cells to express a desired fixed, or time-varying, amount of a reporter protein over thousands of minutes. The computer automatically switched the type of sugar administered to the cells, its concentration and its duration, according to the control algorithm. Our approach can be used to control expression of any protein, fused to a fluorescent reporter, provided that an external molecule known to (indirectly) affect its promoter activity is available.
•Internal approach algorithms significantly outperform external approach ones.•Internal algorithm efficiency is not affected by travel demand model parameters.•Restarting technique significantly ...increases the MSA efficiency.•Smoothing technique together with restarting technique let MSA-CA outperform MSA-FA.•Non-separable and asymmetric arc cost functions do not much affect algorithms efficiency.
Recently, it has been pointed out that transport models should reflect all significant traveler choice behavior. In particular, trip generation, trip distribution, modal split as well as route choice should be modeled in a consistent process based on the equilibrium between transport supply and travel demand. In this paper a general fixed-point approach that allows dealing with multi-user stochastic equilibrium assignment with variable demand is presented. The main focus was on investigating the effectiveness of internal and external approaches and of different algorithmic specifications based on the method of successive averages within the internal approach. The vector demand function was assumed non-separable, non-symmetric cost functions were adopted and implementation issues, such updating step and convergence criterion, were investigated. In particular the aim was threefold: (i) compare the internal and the external approaches; (ii) investigate the effectiveness of different algorithmic specifications to solve the variable demand equilibrium assignment problem through the internal approach; (iii) investigate the incidence of the number of the links with non-separable and/or asymmetrical cost functions. The proposed analyses were carried out with respect to two real-scale urban networks regarding medium-size urban contexts in Italy.
Estimation of age of majority has important applications in the forensic daily practice because of the increasing demand for age estimates of unaccompanied minors. Diagnostic accuracy of I
3M
to ...assess legal adult age of 18 years has been already tested in several specific population samples. The aim of this work was to compare the available data about sensitivity and specificity of the third molar maturity index (I
3M
) from five different regional groups’ radiographic samples to study possible ethnical difference. For this purpose, a sample of 6157 orthopantomograms (OPGs), coming from 15 countries, was analysed. Data about sensitivity and specificity were pooled using a bivariate modelling approach. The one-way MANOVA analysis was applied to assess the likelihood that sensitivity and specificity of the five regional groups are sampled from the same population. The result of the one-way MANOVA showed that both sensitivity and specificity did not depend from the regional groups. The obtained sensitivity was 84% (95% CI: 82% and 85%), and its specificity was 94% (95% CI: 93% and 95%). Based on these results, the I
3M
was a useful statistical tool to identify whether a subject has reached the legal age of 18 years old, regardless of his or her ethnicity.
Regenerative medicine is the branch of medicine that effectively uses stem cell therapy and tissue engineering strategies to guide the healing or replacement of damaged tissues or organs. A crucial ...element is undoubtedly the biomaterial that guides biological events to restore tissue continuity. The polymers, natural or synthetic, find wide application thanks to their great adaptability. In fact, they can be used as principal components, coatings or vehicles to functionalize several biomaterials. There are many leading centers for the research and development of biomaterials in Italy. The aim of this review is to provide an overview of the current state of the art on polymer research for regenerative medicine purposes. The last five years of scientific production of the main Italian research centers has been screened to analyze the current advancement in tissue engineering in order to highlight inputs for the development of novel biomaterials and strategies.
In this work, two procedures for fabrication of polymeric microneedles based on direct photolithography, without any etching or molding process, are reported. Polyethylene glycol (average molecular ...weight 250 Da), casted into a silicone vessel and exposed to ultraviolet light (365 nm) through a mask, cross-links when added by a commercial photocatalyzer. By changing the position of the microneedles support with respect to the vessel, different shapes and lengths can be achieved. Microneedles from a hundred microns up to two millimeters have been obtained just tuning the radiation dose, by changing the exposure time (5-15 s) and/or the power density (9-18 mW/cm²) during photolithography. Different microneedle shapes, such as cylindrical, conic or lancet-like, for specific applications such as micro-indentation or drug delivery, are demonstrated.
Purpose
To assess the use of telemedicine with phone-call visits as a practical tool to follow-up with patients affected by urological benign diseases, whose clinic visits had been cancelled during ...the acute phase of the COVID-19 pandemic.
Methods
Patients were contacted via phone-call and a specific questionnaire was administered to evaluate the health status of these patients and to identify those who needed an “in-person” ambulatory visit due to the worsening of their condition. Secondarily, the patients’ perception of a potential shift towards a “telemedicine” approach to the management of their condition and to indirectly evaluate their desire to return to “in-person” clinic visits.
Results
607 were contacted by phone-call. 87.5% (531/607) of the cases showed stability of the symptoms so no clinic in-person or emergency visits were needed. 81.5% (495/607) of patients were more concerned about the risk of contagion than their urological condition. The median score for phone visit comprehensibility and ease of communication of exams was 5/5; whilst patients’ perception of phone visits’ usefulness was scored 4/5. 53% (322/607) of the interviewees didn’t own the basic supports required to be able to perform a real telemedicine consult according to the required standards.
Conclusion
Telemedicine approach limits the number of unnecessary accesses to medical facilities and represents an important tool for the limitation of the risk of transmission of infectious diseases, such as COVID-19. However, infrastructures, health workers and patients should reach out to a computerization process to allow a wider diffusion of more advanced forms of telemedicine, such as televisit.
In this paper different microscopic discrete event simulation models for a container terminal are presented. The focus is on the best approach to adopt to simulate handling activity time duration and ...on which level of detail should be pursued with respect to different planning horizons that a decision maker have to face. The models share the same logical architecture but differ in the approaches pursued to estimate handling activity time duration. Terminal operations were broken down into elementary activities pursuing a level of disaggregation not usual in the literature; time duration of each elementary handling activity was modelled through a stochastic approach, distinguishing container type; validation was carried out with respect to different planning horizons (real-time/short-term, long-term) through the definition of local and global indicators and a before-and-after analysis. Modelling issues are discussed for tactical and strategic planning, and operational guidelines are given.
Diatom microalgae are the most outstanding natural source of porous silica. The diatom cell is enclosed in a three-dimensional (3-D) ordered nanopatterned silica cell wall, called frustule. The ...unique properties of the diatom frustule, including high specific surface area, thermal stability, biocompatibility, and tailorable surface chemistry, make diatoms really promising for biomedical applications. Moreover, they are easy to cultivate in an artificial environment and there is a large availability of diatom frustules as fossil material (diatomite) in several areas of the world. For all these reasons, diatoms are an intriguing alternative to synthetic materials for the development of low-cost drug delivery systems. This review article focuses on the possible use of diatom-derived silica as drug carrier systems. The functionalization strategies of diatom micro/nanoparticles for improving their biophysical properties, such as cellular internalization and drug loading/release kinetics, are described. In addition, the realization of hybrid diatom-based devices with advanced properties for theranostics and targeted or augmented drug delivery applications is also discussed.
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