Leishmania RNA virus (LRV) is an important virulence factor associated with the development of mucocutaneous Leishmaniasis, a severe form of the disease. LRV-mediated disease exacerbation relies on ...TLR3 activation, but downstream mechanisms remain largely unexplored. Here, we combine human and mouse data to demonstrate that LRV triggers TLR3 and TRIF to induce type I IFN production, which induces autophagy. This process results in ATG5-mediated degradation of NLRP3 and ASC, thereby limiting NLRP3 inflammasome activation in macrophages. Consistent with the known restricting role of NLRP3 for Leishmania replication, the signaling pathway triggered by LRV results in increased parasite survival and disease progression. In support of this data, we find that lesions in patients infected with LRV+ Leishmania are associated with reduced inflammasome activation and the development of mucocutaneous disease. Our findings reveal the mechanisms triggered by LRV that contribute to the development of the debilitating mucocutaneous form of Leishmaniasis.
The GALAH survey: scientific motivation De Silva, G. M; Freeman, K. C; Bland-Hawthorn, J ...
Monthly notices of the Royal Astronomical Society,
05/2015, Letnik:
449, Številka:
3
Journal Article
Recenzirano
Odprti dostop
The Galactic Archaeology with HERMES (GALAH) survey is a large high-resolution spectroscopic survey using the newly commissioned High Efficiency and Resolution Multi-Element Spectrograph (HERMES) on ...the Anglo-Australian Telescope. The HERMES spectrograph provides high-resolution (R ∼ 28 000) spectra in four passbands for 392 stars simultaneously over a 2 deg field of view. The goal of the survey is to unravel the formation and evolutionary history of the Milky Way, using fossil remnants of ancient star formation events which have been disrupted and are now dispersed throughout the Galaxy. Chemical tagging seeks to identify such dispersed remnants solely from their common and unique chemical signatures; these groups are unidentifiable from their spatial, photometric or kinematic properties. To carry out chemical tagging, the GALAH survey will acquire spectra for a million stars down to V ∼ 14. The HERMES spectra of FGK stars contain absorption lines from 29 elements including light proton-capture elements, α-elements, odd-Z elements, iron-peak elements and n-capture elements from the light and heavy s-process and the r-process. This paper describes the motivation and planned execution of the GALAH survey, and presents some results on the first-light performance of HERMES.
Microbial viruses can control host abundances via density-dependent lytic predator-prey dynamics. Less clear is how temperate viruses, which coexist and replicate with their host, influence microbial ...communities. Here we show that virus-like particles are relatively less abundant at high host densities. This suggests suppressed lysis where established models predict lytic dynamics are favoured. Meta-analysis of published viral and microbial densities showed that this trend was widespread in diverse ecosystems ranging from soil to freshwater to human lungs. Experimental manipulations showed viral densities more consistent with temperate than lytic life cycles at increasing microbial abundance. An analysis of 24 coral reef viromes showed a relative increase in the abundance of hallmark genes encoded by temperate viruses with increased microbial abundance. Based on these four lines of evidence, we propose the Piggyback-the-Winner model wherein temperate dynamics become increasingly important in ecosystems with high microbial densities; thus 'more microbes, fewer viruses'.
Carbon dioxide has been identified as one of the main compounds affecting the stability of the earth’s climate. The reduction of the total volume of greenhouse gases emitted to the atmosphere is ...considered a key mechanism to mitigate climate change. Geological storage of CO2 in deep saline aquifers is currently a well-accepted method of storage, because saline aquifers have larger storage capacities than other geological media. Rock–water–CO2 interactions initiated in the aquifer with CO2 injection play a vital role in CO2 sequestration in saline aquifers, and include different trapping mechanisms: geological trapping, hydrodynamic trapping and geo-chemical trapping (solubility trapping and mineral trapping). Of these, geological trapping and solubility trapping are more effective in the short term, but mineral trapping is safer and more economical in the long term. Current knowledge of geochemical trapping is still at an early stage compared to other trapping mechanisms due to the extensive time required to complete the process. To date, very few studies have been conducted on sandstone reservoirs, which are considered to have the largest storage capacity among geological formations. However, due to the long-term safety of CO2 storage with geochemical trapping, there has been a recent trend to research this process. Both solubility and mineral trapping processes in saline aquifers depend on injecting CO2 and the fluid–rock mineral properties of the aquifers. Until very recently, although it was assumed that temperature, pressure, the salinity of the formation water and the mineral composition of the formation rock are the only parameters which affect mineral trapping, recent research has shown that a number of other reservoir parameters, such as layer thickness, tilt angle, anisotropy and bedding pattern may also significantly affect geochemical trapping. This review provides a comprehensive examination of the current knowledge of the geo-chemistry of solubility and mineral trapping processes in deep saline aquifers.
This article proposed to build a system founded on metamaterial sensor antennas, which can be used to evaluate impurities in aqueous substances according to the quality of transmission between the ...sensor antennas. In order to do this, a dedicated setup with tests in several frequencies was deployed so as to monitor the behavior of transmission variation between sensors. These sensors are microstrip antennas with a ground plane of resonant cleaved metallic rings; the substrate functions as a metamaterial for the irradiating element. In this study, an analysis was made of transmission between the sensors, looking for variation in angles of incidence of signal and of distance between the antennas. The sensor was tested at various operating frequencies, as such 1.8 GHz, 2.4 GHz, 3.4 GHz and 4.1 GHz, resulting in different values of sensitivity. The prototypes were constructed and tested so as to analyze the dielectric effects of the impurities on NaCl and C₂H₄O₂ substances. The research aims to use these control systems of impurities in industrial premises.
A variety of cellular responses is needed to ensure the plants survival during drought, but little is known about the signaling mechanisms involved in this process. Soybean cultivars (EMBRAPA 48 and ...BR 16, tolerant and sensitive to drought, respectively) were exposed to the following treatments: control conditions (plants in field capacity), drought (20% of available water in the soil), sodium nitroprusside (SNP) treatment (plants irrigated and treated with 100‐µM SNP SNP–nitric oxide (NO) donor molecule, and Drought + SNP (plants subjected to drought and SNP treatment). Plants remained in these conditions until the reproductive stage and were evaluated for physiological (photosynthetic pigments, chlorophyll a fluorescence and gas exchange rates), hydraulic (water potential, osmotic potential and leaf hydraulic conductivity) and morpho‐anatomical traits (biomass, venation density and stomatal characterization). Exposure to water deficit considerably reduced water potential in both cultivars and resulted in decrease in photosynthesis and biomass accumulation. The addition of the NO donor attenuated these damaging effects of water deficit and increased the tolerance index of both cultivars. The results showed that NO was able to reduce plant's water loss, while maintaining their biomass production through alteration in stomatal characteristics, hydraulic conductivity and the biomass distribution pattern. These hydraulic and morpho‐anatomical alterations allowed the plants to obtain, transport and lose less water to the atmosphere, even in water deficit conditions.
ABSTRACT
We introduce the southern stellar stream spectroscopy survey (S5), an on-going program to map the kinematics and chemistry of stellar streams in the southern hemisphere. The initial focus of ...S5 has been spectroscopic observations of recently identified streams within the footprint of the dark energy survey (DES), with the eventual goal of surveying streams across the entire southern sky. Stellar streams are composed of material that has been tidally striped from dwarf galaxies and globular clusters and hence are excellent dynamical probes of the gravitational potential of the Milky Way, as well as providing a detailed snapshot of its accretion history. Observing with the 3.9 m Anglo-Australian Telescope’s 2-degree-Field fibre positioner and AAOmega spectrograph, and combining the precise photometry of DES DR1 with the superb proper motions from Gaia DR2, allows us to conduct an efficient spectroscopic survey to map these stellar streams. So far S5 has mapped nine DES streams and three streams outside of DES; the former are the first spectroscopic observations of these recently discovered streams. In addition to the stream survey, we use spare fibres to undertake a Milky Way halo survey and a low-redshift galaxy survey. This paper presents an overview of the S5 program, describing the scientific motivation for the survey, target selection, observation strategy, data reduction, and survey validation. Finally, we describe early science results on stellar streams and Milky Way halo stars drawn from the survey. Updates on S5, including future public data releases, can be found at http://s5collab.github.io.
Colour is a key component to enhance the ultimate appetizing value and consumer acceptance towards foods and beverages. Synthetic food colours have been increasingly used than natural food colours by ...food manufacturers to attain certain properties such as low cost, improved appearance, high colour intensity, more colour stability, and uniformity. Varied foods and beverages available in the market may contain some nonpermitted synthetic colours and overuse of permitted synthetic colours. This may lead to severe health problems such as mutations, cancers, reduced haemoglobin concentrations, and allergic reactions. According to the Food Act, 2011 (No. 26 of 1980), Sri Lanka, only nine synthetic food colours are permitted and the colour concentration cannot exceed 100 ppm as a single component or in combination. This study aims to identify the synthetic food colours in confectioneries and beverages available in Jaffna district, Sri Lanka. Randomly collected 110 samples from eleven Medical Officers Of Health areas in Jaffna district were analyzed by using thin layer chromatography and UV-visible spectrophotometry. According to the results, 100% beverages and 85% confectioneries contained permitted synthetic food colours. Out of all, 7% of the confectioneries did not contain any synthetic food colour and 8% of the confectioneries contained nonpermitted colours which do not comply with any of the permitted synthetic food colours. Tartrazine (E102) (41%) was the most used synthetic food colour in both confectioneries and beverages. Moreover, 60% of the beverages violated the label requirement without including proper colour ingredients. The study concluded that there is a high tendency to use synthetic food colours in confectioneries and beverages and some confectioneries contain unidentified colours including a textile dye. Therefore, the implementation of regulations and awareness programs of food colours for consumers and food manufacturers are highly recommended.
This work investigates the ammonia electro-oxidation considering electrochemical and direct ammonia fuel cell (DAFC) experiments. The working electrodes/anodes are composed of Pd/C, PdIr/C (90:10, ...70:30, 50:50, 30:70 and 10:90 atomic ratios) and Ir/C. Solutions of 1 mol L−1 NH4OH and 1 mol L−1 KOH were used for electrochemical experiments while 1.0, 3.0 and 5.0 mol L−1 NH4OH in 1.0 mol L−1 KOH were used in DAFC. X-ray diffraction analysis of PdIr/C electrocatalysts suggests the formation of PdIr alloy, while transmission electron micrographs show the average particle diameters between 4.6 and 6.2 nm. Electrochemical experiments indicate PdIr/C 30:70 as the best electrocatalyst in accordance with DAFC. The maximum power densities obtained with PdIr/C 30:70 as anode using 5 mol L−1 NH4OH and 1 mol L−1 KOH at 40 °C are 60% and 30% higher than the ones obtained with Pd/C and Ir/C electrocatalysts, respectively. The enhanced synergic effect in this specific composition may be assigned to an optimal ratio of palladium sites that dehydrogenates ammonia at lower overpotential with the lower surface coverage of Nads on iridium. Furthermore, electronic effect between palladium and iridium might also contribute to the decrease of poisoning on catalyst surface by Nads.
•Direct ammonia fuel cell (DAFC) performance using PdIr/C electrocatalysts as anode.•A study of different NH4OH concentrations at different fuel cell temperatures.•Using PdIr/C 30:70 were obtained the highest power density and open circuit voltage.
Multifunctional wearable electronic textiles based on interfacial polymerization of polypyrrole on carbon nanotubes/cotton fibers offer advantages of simple and low-cost materials that incorporate ...bactericidal, good electrochemical performance, and electrical heating properties. The high conductivity of doped polypyrrole/CNT composite provides textiles that reaches temperature on order of 70 °C with field of 5 V/cm, superior electrochemical performance applied as electrodes of supercapacitor prototypes, reaching capacitance in order of 30 F g–1 and strong bactericidal activity against Staphylococcus aureus. The combination of these properties can be explored in smart devices for heat and microbial treatment on different parts of body, with incorporated storage of energy on textiles.
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