The two varieties of mangosteen (
L.) cultivated in Malaysia are known as Manggis and Mesta. The latter is preferred for its flavor, texture, and seedlessness. Here, we report a complete plastome ...(156,580 bp) of the Mesta variety that was obtained through a hybrid assembly approach using PacBio and Illumina sequencing reads. It encompasses a large single-copy (LSC) region (85,383 bp) and a small single-copy (SSC) region (17,137 bp) that are separated by 27,230 bp of inverted repeat (IR) regions at both ends. The plastome comprises 128 genes, namely, 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The plastome of the Manggis variety (156,582 bp) obtained from reference-guided assembly of Illumina reads was found to be nearly identical to Mesta except for two indels and the presence of a single-nucleotide polymorphism (SNP). Comparative analyses with other publicly available
plastomes, including
,
,
var. Thailand,
,
, and
, found that the gene content, gene order, and gene orientation were highly conserved among the
species. Phylogenomic analysis divided the six
plastomes into three groups, with the Mesta and Manggis varieties clustered closer to
,
, and
, while the Thailand variety clustered with
in another group. These findings serve as future references for the identification of species or varieties and facilitate phylogenomic analysis of lineages from the
genus to better understand their evolutionary history.
•Diagnostics for MeYVMV in mesta (Hibiscus sabdariffa L. and H. cannabinus L.) by loop-mediated isothermal amplification assay was developed.•LAMP assay developed in this study for the detection of ...MeYVMV in mesta is more sensitive than PCR technique.•The LAMP assay has been optimized using Hydroxy Naphthol Blue (HNB) dye is more suitable for field sample diagnosis and in quarantine.•This is the first report for detection of begomovirus species, MeYVMV in the mucilaginous plant species, kenaf and roselle using LAMP.
A loop-mediated isothermal amplification (LAMP) assay was optimized for the detection of Mesta yellow vein mosaic virus (MeYVMV) in diseased plants of mesta (Hibiscus sabdariffa L.& H. cannabinus L.). The LAMP assay was optimized using a set of six primers targeting the MeYVMV genome and could be completed in 30–60 min at 63 °C. The LAMP amplification results were visualized by adding 1 μl of hydroxy naphthol blue (HNB) dye in a 25 μl LAMP reaction mixture prior to amplification as well as by electrophoresis. The LAMP assay, which detected MeYVMV in a 10−5-fold diluted total DNA, was more sensitive than the PCR assay (10−4-fold dilution). The optimized LAMP assay was able to detect MeYVMV in different parts of the kenaf and roselle plants. Similarly, the optimized PCR assay was also capable of detecting MeYVMV in all the different parts of the kenaf plant but failed to detect the virus in the stem and flower buds of the roselle plant. Validation of the LAMP and LAMP with HNB dye assays revealed that the optimized reactions can be used successfully for the in-situ detection of MeYVMV in field samples and in virus quarantine programs. This is the first report of the detection of the begomovirus species, MeYVMV, in the mucilaginous plant species, kenaf and roselle, using a LAMP assay.
Namen članka je predstaviti kakovost družbene infrastrukture izbranih kratkih oskrbnih prehranskih verig v Mestni občini Ljubljana in njen vpliv na varovanje virov, zmanjšanje odpadne hrane in ...embalaže. V raziskavi smo obravnavali šest ponudnikov, vključenih v kratke oskrbne prehranske verige z zabojčki, skupnim naročanjem in partnerskim kmetovanjem. Presoja objektov s pomočjo smernic trajnostne gradnje BNB (Bewertungssystem Nachhaltiges Bauen) je pokazala slabo kakovost in pomanjkljivo družbeno infrastrukturo. V pogovoru z intervjuvanci smo spoznali, da si želijo urejena in lahko dostopna prevzemna mesta z objekti, ki bi omogočali dobre pogoje za ljudi in živila. Mestna občina Ljubljana ima glede na bližnje podeželje z dobrimi pogoji za ekstenzivno in ekološko kmetijsko pridelavo še veliko možnosti za povečanje samooskrbe s kakovostno domačo hrano, ob tem pa bo morala poskrbeti za razvoj družbene infrastrukture.
Mesta crop biomass is one of the unexplored lignocellulosic feedstocks for second-generation bioethanol production. The compositional analysis of two mesta species, namely Hibiscus sabdariffa and H. ...cannabinus were carried out which revealed that the mesta contains 41.89-46.50% cellulose that can be saccharified to release fermentative sugar. Among different physical and physico-chemical pretreatment methods employed, cold alkali (2%) treatment showed maximum cellulose enrichment (38.71%) and significant lignin removal (27.27% of total). Optimization of saccharification process for pretreated mesta biomass was carried out using Box-Behnken design. Based on the observations, use of 4.02% substrate loading, 15.02 FPU/mL of cellulase from psychrotolerant Aspergillus niger SH3, and 5.01 IU/gds of β-glucosidase from Pseudomonas lutea BG8, led to 533.25 mg/gds sugar release from the pretreated biomass. Simultaneous saccharification and fermentation was carried out using efficient thermotolerant yeast strain Saccharomyces cerevisiae LN that resulted in 4.1 g/L ethanol production. It can be concluded that mesta crop can be a good source for the production of second-generation biofuel and the ethanol yield can be further improved by use of accessory enzymes and co-fermenting yeast.
New sensitive high-resolution ion microprobe (SHRIMP) UPb zircon geochronologic data, whole-rock geochemical and Sr-Nd-Pb isotopic data, and zircon and quartz δ18O isotopic data from the Staré Město ...granitoids (SMG), Jawornik granitoids (JG), and Kłodzko-Złoty Stok granitoids (KZSG) and associated mafic and ultramafic rocks are examined. This study provides new insights into the processes of magma generation, transport and emplacement during the Variscan development of the contact zone of the Saxothuringia, Teplá-Barrandia and Brunovistulia in the Central European portion of the Variscan belt. The results of this study, combined with existing geochemical and isotopic data, imply that the parental magmas of these intrusions share a close affinity and suggest that these intrusions formed in a subduction-related tectonic setting. The SMG, JG and KZSG magmas represent hybrids that formed from the contamination of partial melts from the lower crust and/or subducted sediments with various proportions of enriched mantle-derived melts. These mainly sheeted plutons intruded along the main geological boundaries during the Visean and recorded the vertical and lateral displacements between the major tectono-stratigraphic units (microplates) in the NE Bohemian Massif. In the Central Sudetes, these granitoid magmas were first emplaced along the northern continuation of the Moldanubian Zone. The SHRIMP UPb dating of zircons indicates that the SMG intruded the reactivated suture zone between the Brunovistulia and Saxothuringia at 344–341 Ma. Sills of the JG were emplaced between c. 347 and c. 334 Ma. The composite Kłodzko-Złoty Stok Pluton, which includes the KZSG and accompanying mafic enclaves and pyroxenite and lamprophyre dykes, was mainly emplaced at 340–333 Ma. The magmas of the KZSG possibly facilitated the final amalgamation of the Sudetic counterparts of the Teplá-Barrandia and Saxothuringia microplates during the orogenic uplift of the latter. Petrologic and oxygen isotopic data further indicate partial post-magmatic hydrothermal and/or alteration processes. Our new data further stress the connection of magma transfer and active shear zones, which could manifest as crustal-scale magma-ascent conduits.
•Magmatic activity is an integral part of Variscan orogenic processes in the NE Bohemian Massif•U-Pb ages and Sr-Nd-Pb-O isotopes of Kłodzo-Złoty Stok, Jawornik and Staré Město plutons are presented•Multi-method study suggests subduction-related hybrid magmatism during Visean.•Sheeted plutons intruded major shear zones in the Central Sudetes starting at ~344 Ma.•Active shear zones can serve as channels for the crustal-scale transport of melt.
El presente estudio analiza una estadística realizada para averiguar las roturaciones de tierras comunes que se habían realizado en perjuicio de los intereses de la Mesta en la primera mitad del ...siglo XVIII. Pero se sugiere una aproximación al documento desde el punto de vista del análisis textual basado en el valor de metatexto de la estadística y mediante el cual se puede valorar el significado político e histórico de los datos cuantitativos con apuntes sobre el sentido del gobierno de la Ilustración.
Mesta bonded nonwoven of 120 g/m
2
with poly-vinyl alcohol was made using impregnation-squeezing - drying-curing method. The effect of three process parameters adhesive concentration, squeezing ...pressure and curing temperature on the add-on and mechanical properties of the fabric, i.e. tenacity, breaking elongation, initial modulus, energy-to-break, stress relaxation, creep, bursting strength and tearing strength, have been studied using 20 points central composite rotatable design. All these properties have a significant effect on the performance of fabric in different uses. Second-order polynomials have been proposed with a good correlation for all these properties. From these models and contour diagrams, the effects of different parameters on mechanical properties have been discussed elaborately. Moreover, the models can also predict the properties satisfactorily knowing the values of factors or parameters. The predicted value show good agreement with observed values. The information available from these models and subsequent contour diagrams regarding the interactions of parameters on properties are very much useful to design a mesta adhesive-bonded nonwoven fabric. The critical point must be taken care of for designing a fabric for particular end use. To get the optimum mechanical properties of 120 g/m
2
fabric, the process parameters will be 20% adhesive concentration, 1.2 N/cm
2
squeezing pressure and 120 °C curing temperature with the same experimental setup and other parameters.
Introduction: Cancer is causing death in countries with more or less economic development, and it is expected that the burden will grow worldwide, especially in countries with less developed ...populations, where about 82% of the global population lives. The aim of this study was to present and estimate trends in incidence, mortality rate and MIR of malignant neoplasm of otherand ill-defined sites of lip, oral cavity and pharynx cancer in Central Serbia from 1999 to 2020. Material and methods: The registry-based study was conducted using the data from the publically accessible Yearbooks of the Institute of Public Health of Serbia: Incidence and Mortality in Central Serbia, from 1999 to 2020. Joinpoint regression was used to determine the trend and annual percentage change (APC) of the incidence and mortality rate with corresponding 95% confidence intervals. Results: A total number of 558 cases (436men and 122women) of other and ill-defined lip, oral cavity and pharynx cancer sites registered in Central Serbia from 1999 to 2020. The crude rate (CR) and age-standardized rate (ASR-W) of incidence in males increased (p<0.001, for both) during the study period with APC of 4.8% and 9.0%, respectively. The crude rate (CR) and age-standardized rate (ASR-W) of mortality in males decreased (p<0.001, for both) during the study period with APC of -7.5%, and -7.2%, respectively. The mortality rate decrease was followed by the decline in MIR between 2001 and 2020 with anAPC of -5.6 (p<0.001). Conclusions: Our results reveal that the incidence of other and ill-defined lip, oral cavity and pharynx cancer sites in Central Serbia increased through the study period. The mortality rate and MIR for this cancer decreased during 22 years.
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