To evaluate the expression of hCTLA4-Ig and their biological function in newborn porcine islets (NPIs) transfected with AAV-hCTLA4-Ig.
Cultured NPIs were transfected with AAV-hCTLA4-Ig. The ...expression of CTLA4-Ig in these NPIs was assayed by RT-PCR and immunocytochemistry. The levels of IL-2, IFN-gamma, and TNF-alpha in the culture medium were assayed by ELISA after these cells the co-cultured with human. The response of glucose-stimulated insulin secretion was observed in the transgene group and the control group.
The expressions of CTLA4-Ig mRNA and protein were detected in the transgene group. The levels of cytokines were obviously lower in the transgene group than those in the control group (P<0.01). There was no significant difference in the response of glucose-stimulated insulin release between the transgene group and the control group (P>0.05).
AAV mediated hCTLA4-Ig expression in NPIs could inhibit T lymphocyte to produce cytokines, while the endocrine functions of the NPIs were not significantly affe
Introduces use of grey literature in Sumitomo Metal Industry K.K. The Company has consigned its information management and processing to its associated company, Sumikin Cosmo Plans K.K. Describes the ...outline of Sumikin Cosmo Plans, and the service contents and use of New Policy Information Service (NPIS) there, a membership service. Also describes processing and services of corporate materials and conference proceedings conducted at the Document Center of the Sumitomo Metal Industry K.K., and clearing services there concerning information which they find it difficult to identify among their holdings and they contact to extemal specialized libraries for obtaining.
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