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•Trametes versicolor is capable of simultaneous biodegrading CAP and removing Cd.•Nine transformation products (TPs) were identified, including six novel TPs.•Two novel CAP ...biodegradation pathways were proposed through the TPs.•Biodegradation and adsorption were the main mechanism of CAP removal by T. versicolor.•Cellular accumulation and surface adsorption were the main mechanism of Cd removal.
The recalcitrant chloramphenicol (CAP) combined with heavy metals cadmium (Cd) commonly co-existed in the environment, posing threat to environment health. The capacity of Trametes versicolor to remove/biodegrade CAP in air-pulse fluidized-bed reactor was evaluated, even under Cd stress. T. versicolor could remove 44 % CAP of 5 mg/L in 15 days, even 51 % CAP under 1 mg/L Cd stress. Sustained Cd stress inhibited CAP biodegradation and Cd removal in a 5-batches of a 5-days cycle sequential batch reactor. Nine transformation products and two novel pathways were proposed, with initial multi-step transformation reaction into CP2 and allylic alcohol, respectively. Furthermore, the main mechanism of Cd removal by T. versicolor was extracellular surface bioadsorption and intracellular accumulation. This study filled the gap of the mechanism of simultaneous CAP removal/biodegradation and Cd removal by white-rot fungi T. versicolor, which offer a theoretical basis for future application of biological removal of CAP containing wastewater.
•32 out of 38 antibiotics were removed >50% after 24h by enzymatic treatment.•Laccase in combination with syringaldehyde (SYR) effectively removed antibiotics.•No significant reduction of antibiotics ...with laccase without any mediator.•The addition of SYR to laccase resulted in a time-dependent increase of toxicity.
In this study, the performance of immobilised laccase (Trametes versicolor) was investigated in combination with the mediator syringaldehyde (SYR) in removing a mixture of 38 antibiotics in an enzymatic membrane reactor (EMR). Antibiotics were spiked in osmosed water at concentrations of 10μg·L−1 each. Laccase without mediator did not reduce the load of antibiotics significantly. The addition of SYR enhanced the removal: out of the 38 antibiotics, 32 were degraded by >50% after 24h. In addition to chemical analysis, the samples’ toxicity was evaluated in two bioassays (a growth inhibition assay and the Microtox assay). Here, the addition of SYR resulted in a time-dependent increase of toxicity in both bioassays. In cooperation with SYR, laccase effectively removes a broad range of antibiotics. However, this enhanced degradation induces unspecific toxicity. If this issue is resolved, enzymatic treatment may be a valuable addition to existing water treatment technologies.
Laccases have wide range of substrate specificity and find applications from pulp industry to waste water remediation. Laccases have also been used in combined pretreatment of biomass hydrolyzates to ...remove enzymatic and fermentation inhibitors. In this study, laccase production by Trametes versicolor strains isolated from different regions of the United States was induced using copper salts. T. versicolor crude culture filtrates (CCF), without any purification step, were tested for removal of model inhibitor compounds as well as in poplar and rice straw pretreatment hydrolyzates. Phenolic inhibitors were removed by 76% and 94% from the dilute acid hydrolyzates of rice straw and poplar, respectively, when incubated with the CCF for 12h, at room temperature. Xylo-oligosaccharide concentrations present in rice straw hydrolyzates were reduced by 64% when incubated with T. versicolor CCF. T. versicolor CCF could be a low cost technology for decreasing enzymatic and fermentation inhibitors.
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•TX100 or RL had significant influence on the interactions of Lac with phenol.•Both surfactants changed the binding site of Lac bound to phenol.•Single TX100 or RL decreased the ...binding energy between phenol and Lac.•The behavior of water molecules was also transformed due to the formation of HBs.
Some surfactants can enhance the removal of phenol by laccase (Lac) in various industrial effluents. Their behavior and function in the biodegradation of phenolic wastewater have been experimentally reported by many researchers, but the underlying molecular mechanism is still unclear. Therefore, the interaction mechanisms of phenol with Lac from Trametes versicolor were investigated in the presence or absence of Triton X-100 (TX100) or rhamnolipid (RL) by molecular docking and molecular dynamics (MD) simulations. The results indicate that phenol contacts with an active site of Lac by hydrogen bonds (HBs) and van der Waals (vdW) interactions in aqueous solution for maintaining its stability. The presence of TX100 or RL results in the significant changes of enzymatic conformations. Meanwhile, the hydrophobic parts of surfactants contact with the outside surface of Lac. These changes lead to the decrease of binding energy between phenol and Lac. The migration behavior of water molecules within hydration shell is also inevitably affected. Therefore, the amphipathic TX100 or RL may influence the phenol degradation ability of Lac by modulating their interactions and water environment. This study offers molecular level of understanding on the function of surfactants in biosystem.
The elimination of 81 pharmaceuticals (PhACs) by means of a biological treatment based on the fungus Trametes versicolor was evaluated in this work. PhAC removal studied in different types of ...wastewaters (urban, reverse osmosis concentrate, hospital, and veterinary hospital wastewaters) were reviewed and compared with conventional activated sludge (CAS) treatment. In addition, hazard indexes were calculated based on the exposure levels and ecotoxicity for each compound and used for the evaluation of the contaminants removal. PhAC elimination achieved with the fungal treatment (mean value 76%) was similar or slightly worse than the elimination achieved in the CAS treatment (85%). However, the fungal reactor was superior in removing more hazardous compounds (antibiotics and psychiatric drugs) than the conventional activated sludge in terms of environmental risk reduction (93% and 53% of reduction respectively). Fungal treatment can thus be considered as a good alternative to conventional treatment technologies for the elimination of PhACs from wastewaters.
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•Efficiency of fungal treatment was evaluated in different wastewater effluents.•Removal of up to 81 pharmaceuticals in all treatments was estimated.•Reduction of environmental risk after fungal treatment was also assessed.•Antibiotics and psychiatric drugs were better removed by fungal treatment.•Fungal treatment showed promising results from an ecotoxicological point of view.
The biomixture is the major constituent of a biopurification system and one of the most important factors in its efficiency; hence the selection of the components is crucial to ensure the efficient ...pesticides removal. Besides, bioaugmentation is an interesting approach for the optimization of these systems.
A mixed culture of the fungus Trametes versicolor SGNG1 and the actinobacteria Streptomyces sp. A2, A5, A11, and M7, was designed to inoculate the biomixtures, based on previously demonstrated ligninolytic and pesticide-degrading activities and the absence of antagonism among the strains. The presence of lindane and/or the inoculum in the biomixtures had no significant effect on the development of culturable microorganisms regardless the soil type. The consortium improved lindane dissipation achieving 81–87% of removal at 66 d of incubation in the different biomixtures, decreasing lindane half-life to an average of 24 d, i.e. 6-fold less than t1/2 of lindane in soils. However, after recontamination, only the bioaugmented biomixture of silty loam soil enhanced lindane dissipation and decreased the t1/2 compared to non-bioaugmented. The biomixture formulated with silty loam soil, sugarcane bagasse, and peat, inoculated with a fungal-actinobacterial consortium, could be appropriate for the treatment of agroindustrial effluents contaminated with organochlorine pesticides in biopurification systems.
•Lindane did not affect the growth of culturable microorganisms in the biomixtures.•Bioaugmentation improved lindane dissipation in the biomixtures of the three soils.•The consortium decreased lindane t1/2 to 6-fold less than t1/2 reported for soils.•After recontamination only the inoculated silty loam-biomixture reduced the t1/2.•This is the first report on fungal-actinobacterial bioaugmentation of biomixtures.
The contribution of the sorption processes in the elimination of pharmaceuticals (PhACs) during the fungal treatment of wastewater has been evaluated in this work. The sorption of four PhACs ...(carbamazepine, diclofenac, iopromide and venlafaxine) by 6 different fungi was first evaluated in batch experiments. Concentrations of PhACs in both liquid and solid (biomass) matrices from the fungal treatment were measured. Contribution of the sorption to the total removal of pollutants ranged between 3% and 13% in relation to the initial amount. The sorption of 47 PhACs in fungi was also evaluated in a fungal treatment performed in 26days in a continuous bioreactor treating wastewater from a veterinary hospital. PhACs levels measured in the fungal biomass were similar to those detected in conventional wastewater treatment (WWTP) sludge. This may suggest the necessity of manage fungal biomass as waste in the same manner that the WWTP sludge is managed.
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•Analytical methodology for PhACS in fungal biomass was developed.•Pharmaceuticals sorbed in the biomass in degradation experiments was measured.•Between 3 and 13% of PhACs elimination can be attributed to sorption processes.•Accumulation of PhACs in fungi is similar to that in sludge of conventional treatments.
Humic constituents (HCs) are ubiquitous in the aquatic ecosystems, and contain various functional groups that seriously impact the conversion of 17β-estradiol (17β-E2) by fungal laccase. The purpose ...of this study was to explore the influencing mechanism of HCs on Trametes versicolor laccase-enabled 17β-E2 oxidation and oligomerization. Herein, T. versicolor-secreted laccase could rapidly convert 99.2% of 17β-E2 (rate constant = 3.7 × 10−2 min−1, half-life = 18.7 min) into multifarious oligomers at 25 °C and pH 5.0, by phenolic radical-caused C–C and/or C–O self-linking routes, whereas HCs with O-phenolic hydroxyl groups (O-p-OH, i.e., catechol, pyrogallol, gallic acid, and caffeic acid) dramatically suppressed 17β-E2 oligomerization. Compared with HC-free, 17β-E2 rate constants weakened 6.3–15.8 fold in the presence of HCs containing O-p-OH. It is largely because the O-p-OH was preferentially oxidized by T. versicolor laccase to create the electrophilic O-quinone monomers/oligomers. These unstable reactive O-quinone intermediates strongly reversed 17β-E2 phenolic radicals to their monomeric molecules via two proton-transfer versus two electron-transfer channels, thus intercepting 17β-E2 oxidation and oligomerization. These findings highlight new insights into the effect of HCs containing O-p-OH on T. versicolor laccase-started 17β-E2 conversion, which is beneficial to re-understanding the fate and geochemical behavior of 17β-E2 in water.
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•Trametes versicolor laccase-started bioremediation could rapidly convert 17β-estradiol.•Multifarious oligomers of 17β-estradiol and humic constituents were identified.•Inhibition mechanism of humic constituents on 17β-estradiol oxidation was illustrated.
Use of agrochemicals is a worldwide practice that exerts an important effect on the environment; therefore the search of approaches for the elimination of such pollutants should be encouraged. The ...degradation of the insecticides imiprothrin (IP) and cypermethrin (CP), the insecticide/nematicide carbofuran (CBF) and the antibiotic of agricultural use oxytetracycline (OTC) were assayed with the white rot fungus Trametes versicolor. Experiments with fungal pellets demonstrated extensive degradation of the four tested agrochemicals, at rates that followed the pattern IP>OTC>CP>CBF. In vitro assays with laccase-mediator systems showed that this extracellular enzyme participates in the transformation of IP but not in the cases of CBF and OTC. On the other hand, in vivo studies with inhibitors of cytochrome P450 revealed that this intracellular system plays an important role in the degradation of IP, OTC and CBF, but not for CP. The compounds 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (DCCA) and 3-phenoxybenzoic acid (PBA) were detected as transformation products of CP, as a result of the breakdown of the molecule. Meanwhile, 3-hydroxycarbofuran was detected as a transformation product of CBF; this metabolite tended to accumulate during the process, nonetheless, the toxicity of the system was effectively reduced. Simultaneous degradation of CBF and OTC showed a reduction in toxicity; similarly, when successive additions of OTC were done during the slower degradation of CBF, the fungal pellets were able to degrade both compounds. The simultaneous degradation of the four compounds successfully took place with minimal inhibition of fungal activity and resulted in the reduction of the global toxicity, thus supporting the potential use of T. versicolor for the treatment of diverse agrochemicals.
•Trametes versicolor efficiently degrades four different agrochemicals simultaneously.•Order of degradation rates is: imiprothrin>oxytetracycline>cypermethrin>carbofuran.•Degradation process usually decreases the initial toxicity of the agrochemicals.•Cytochrome P450 is involved in the degradation, except for cypermethrin.•Laccase plays a role in the transformation of imiprothrin.
•Konjac flying powder extract and its active compounds affect biochemistry of wood decay fungi.•Extract inhibit enzyme activity, respiratory and energy metabolism of wood decay fungi.•Salicylic acid, ...vanillin or cinnamaldehyde inhibits enzyme activity and damage cell membrane of wood decay fungi.•2,4,6-trichlorophenol inhibits enzyme activity and respiratory metabolism of wood decay fungi.
Wood products are vulnerable to fungal degradation that reduces service life. Traditional wood preservatives can provide excellent service life but efforts are underway to develop more natural methods for wood protection. Konjac flying powder is a residual waste produced during the processing of corms of Konjac (Amorphophallus konjac K. Koch) to produce Konjac flour. This waste material contains a number of compounds including salicylic acid (SA), 2,4,6-trichlorophenol (TCP), vanillin (VL), and cinnamaldehyde (CMA) has been found to be active against decay fungi; however, the antifungal mode of action is uncertain. The effects of konjac flying powder extract (KFPE) and its active compounds on cellulase, hemicellulose and ligninase activity, respiratory metabolism, cell membrane permeability, protein, and energy metabolism was studied on the white-rot fungus, Trametes versicolor (L. ex Fr.) Quél. (T. versicolor) and brown-rot fungus, Gloeophyllum trabeum (Pers.: Fr.) Murr. (G. trabeum). Konjac flying powder inhibited cellulase and hemicellulase activity of both fungi and ligninase activity of T. versicolor. Respiratory metabolism and energy metabolism were also inhibited. The four active compounds had different effects on activities suggesting that konjac flying powder functioned against multiple metabolic activities of the test fungi, potentially increasing its ability to provide broad spectrum wood protection.
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