In this study,
graft polymerization of ε-caprolactone (CL) was studied for its potential to limit boric acid (BA) leaching from wood, and to increase the dimensional stability and biological ...durability of wood against
and
fungi. For this purpose, CL monomer with/without BA was impregnated into wood, and the monomer was polymerized in wood cell walls using various methods and combinations of reactants. It was found that biodegradable polycaprolactone (PCL) grafting at high monomer concentrations could inhibit fungal degradation of wood cell walls, as well as improve the dimensional stability of wood up to 55% and reduce water uptake (WU) by about 50%. However, inductively coupled plasma-optical emission spectrometry (ICP-OES) analysis revealed that
graft polymerization of CL is not an effective method to prevent BA leaching from wood.
Phytopathogenic fungi can cause plant diseases that are difficult to control, including mass mortality of some tree species. The Fusarium oxysporum complex (sensu lato) is one of the most dangerous ...groups of phytopathogenic fungi, causing the death of conifer species, including Pinus sylvestris seedlings in forest and ornamental nurseries. Recently, non-chemical methods of plant protection have become the basis of integrated pest management (IPM) in the European Union (EC Directive). The possibility of protection of pine seedlings against the pathogen F. oxysporum using active substances from wood-destroying fungi commonly found in forests was examined. Methanolic extracts of Fomitopsis pinicola, Ganoderma applanatum, and Trametes versicolor were found to contain substances effective in both prevention and treatment of infected seedlings. G. applanatum and T. versicolor showed particular biological activity in increasing plant resistance. Efficacy, especially of the extract of F. pinicola, increased with concentration. Further field trials are needed to confirm the results obtained in laboratory tests on plant protection.
This study aimed to estimate antiradical, antioxidant (AO) and cytotoxic activities of the fungus Trametes versicolor ethanol fruiting body extract. The extract was found to effectively scavenge both ...O
2
*−
and NO
*
(29.62 and 52.48 μg/mL, respectively). It also showed a good AO activity in the polarographic HPMC assay (950%/mL). p-Hydroxybenzoic acid may be one of the responsible compounds for the afore-mentioned activities. The same extract also exhibited a concentration-dependent cytotoxicity against MCF-7 and HepG2 tumour cell lines reaching IC
50
values of 123.51 and 134.29 μg/mL, respectively with no cytotoxic activity against normal MRC-5 cells. Gentisic, syringic and protocatechuic acids may be among the bioactive principles for the observed cytotoxicity. Taken all together, T. versicolor ethanol extract can be considered as a promising candidate for development of health promoting food supplement.
In the bioethanol industry and its derivative processes, 9–14 l of vinasse per liter of ethanol are obtained as residue, depending on whether or not a recirculation process is used. If the vinasse is ...discharged directly into water bodies without proper treatment it can produce negative effects on existing biota and on human health. The main objective of this study was to evaluate the capacity of a mixed treatment with Komagataeibacter kakiaceti GM5 and Trametes versicolor DSM 3086 to degrade and reduce the toxicity of this by-product. In order to carry out the tests, an experimental culture media of vinasse at 60% (v/v) was used. This culture was compared to a standard medium of glucose at 2% and, simultaneously, a mixed culture (bacterium-fungus) was used through a batch process in an orbital shaker. When compared to the treatments evaluated with individually grown microorganisms, the mixed treatment proposed produced the best COD results in vinasse degradation. However, better results were obtained when using the treatment with T. versicolor DSM 3086 for color removal and treatment with K. kakiaceti GM5 produced better results in terms of turbidity and toxicity. Although the simultaneous mixed treatment did not improve all the variables studied, it never showed the lowest performance. Therefore, this proposal can be considered as an innovative biological alternative in degradation processes of great organic pollutants such as vinasse.
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•The vinasse diluted to 60% (v/v), was considered as the optimal concentration.•The mix treatment presented the best degradation of vinasse in terms of COD.•K. kakiaceti GM5 was able to decrease the acute toxicity up to 11.9%.•The mixed treatment presents a novel alternative for the degradation of the vinasse.
Bioethanol from oil palm empty fruit bunches as a second-generation bioethanol (bioethanol G2) has been developed. It produces high amounts of black liquor wastewater which also contains alkaline ...solution (NaOH). Black liquor wastewater is harmful to aquatic ecosystems if discharge directly into waters. This study aimed to determine decolorization of black liquor wastewater by using chemical and biological methods. Coagulation-flocculation method by using Poly Aluminium Chloride (PAC) and alumwith different ratio was also investigated. This study also identified the ability of white-rot fungi to decolorize black liquor wastewater on agar and liquid medium.The results showed that black liquor wastewater with initial concentration 30,000ppm can be decolorized by using two methods, coagulation-flocculation and decolorization by white-rot fungi.The optimum condition to obtain 96% decolorization was using concentration coagulant 1% combination alum-PAC 3:1 with total retention time 33minutes.Out of the three fungi studied, Trametes versicolor U97 showed the most degraded fungus onagar medium containing black liquor. In liquid medium, the percentage of decolorization ranged from 70% to 89% at 30 days depending on the fungal strain.
Emerging contaminants are a wide group of chemical products that are found at low concentrations in the environment. These contaminants can be either natural, e.g., estrogens, or synthetics, such as ...pesticides and pharmaceuticals, which can enter the environment through the water and sludge from wastewater treatment plants (WWTP). The growth of Trametes versicolor on membrane biological reactor (MBR) sludge in bioslurry systems at the Erlenmeyer scale was assessed and its capacity for removing pharmaceutical and personal care products (PPCPs) was evaluated. The ability of the fungus to remove hydrochlorothiazide (HZT) from liquid media cultures was initially assessed. Consequently, different bioslurry media (complete nutrient, glucose and no-nutrient addition) and conditions (sterile and non-sterile) were tested, and the removal of spiked HZT was monitored under each condition. The highest spiked HZT removal was assessed under non-sterile conditions without nutrient addition (93.2%). Finally, the removal assessment of a broad set of pharmaceuticals was performed in non-spiked bioslurry. Under non-sterile conditions, the fungus was able to completely degrade 12 out of the 28 drugs initially detected in the MBR sludge, achieving an overall degradation of 66.9%. Subsequent microbial analysis showed that the microbial diversity increased after 15 days of treatment, but there was still some T. versicolor in the bioslurry. Results showed that T. versicolor can be used to remove PPCPs in bioslurry systems under non-sterile conditions, without extra nutrients in the media, and in matrices as complex as an MBR sludge.
This paper describes electrochemical behavior of laccase from the fungus Trametes versicolor. The issues related to discrimination of the redox potentials corresponding to copper centers T1 and T2/T3 ...in the active site and possible mechanism of intramolecular electron transfer have been discussed. The electron‐transfer rate constant for laccase immobilized on carbon electrode is 3.4 s−1. The bioelectrocatalytic activity of the enzyme was studied in the presence of 1,4‐hydroquinone (HQ). The kinetics of HQ oxidation is very fast (KM=3.8 μM). However, the catalytic activity of laccase in the presence of high concentration of HQ decreases drastically. It is suggested that the T2/T3 copper center is able to accept electrons from HQ molecules directly via intramolecular channel.
The lack of a commercially available robust and inexpensive laccase is a major barrier to the widespread application of this enzyme in various industrial sectors. By using an efficient system ...developed in Streptomyces lividans, we have produced by homologous expression 350 mg L⁻¹ of a bacterial laccase with a high purity and without any extensive purification. This is the highest production yield reported in the literature for a bacterial laccase. The secreted enzyme achieved oxidation under a wide pH range depending on the substrate: 4.0 for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) and 9.0 for 2,6-dimethoxyphenol. Furthermore, this bacterial laccase was found to be quite resistant under various conditions. It withstands pH from 3.0 to 9.0, shows a great thermostability at 70°C and was highly resistant toward conventional inhibitors. For instance, while the laccase of Trametes versicolor was completely inhibited by 1 mM NaN₃, the laccase of Streptomyces coelicolor was fully active under the same conditions. To assess application potential of this laccase, we have investigated its ability to decolourise Indigo carmine. This enzyme was able to rapidly decolourise the dye in the presence of syringaldehyde as a redox mediator.
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► Enzymatic membranes prepared by grafting laccases onto ceramic supports. ► Phenolic compounds depletion from aqueous solutions by enzymatic membrane. ► Membrane plugging phenomena ...by polymers formed from phenols degradation. ► Enzymatic membranes well adapted for low reactive phenols compounds depletion.
This work describes the optimization and characterization of enzymatic membranes assessed for the degradation of two phenolic compounds (2,6-dimethoxyphenol (DMP) and guaiacol) by laccases from Trametes versicolor using the “flow-through membrane reactor” concept. The results obtained show that the optimized membrane is very reactive, although polymer formation during DMP degradation causes membrane plugging that subsequently decreases permeate flow. Nevertheless, the enzymatic membrane can be of interest for much diluted substrates or for relatively un-reactive reaction intermediates that do not cause significant membrane plugging by polymer formation. This work opens new perspectives for the application of enzymatic membranes for the removal of refractory pollutants containing phenolic groups.
The incidence and mortality of colorectal cancer have shown an upward trend in the past decade. Therefore, the prevention, diagnosis, and treatment of colorectal cancer still need our continuous ...attention. Finding compounds with strong anticancer activity and low toxicity is a good strategy for colorectal cancer (CRC) therapy. Trametes versicolor is a traditional Chinese medicinal mushroom with a long history of being used to regulate immunity and prevent cancer. Its extractions were demonstrated with strong cell growth inhibitory activity on human colorectal tumor cells, while the anticancer activity of them is not acted through a direct cytotoxic effect. However, the intricacy and high molecular weight make mechanistic research difficult, which restricts their further application as a medication in clinical cancer treatment. Recent research has discovered a small molecule polysaccharide peptide derived from Trametes versicolor that has a distinct structure after decades of Trametes versicolor investigation. Uncertain molecular weight and a complex composition are problems that have been solved through studies on its structure, and it was demonstrated to have strong anti-proliferation activity on colorectal cancer in vitro and in vivo via interaction with EGFR signaling pathway. It opens up new horizons for research in this field, and these low molecular weight polysaccharide peptides provide a new insight of regulation of colorectal cancer proliferation and have great potential as drugs in the treatment of colorectal cancer.
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