Root-knot nematodes (Meloidogyne spp.) are important pests of many cultivated plants. Recently, the most efficient chemical control products (e.g. methyl bromide) have now been restricted due to ...their toxic characteristics. Research on agents that work against root-knot nematodes and do not have a detrimental impact on the environment is becoming increasingly important. Advances in the last decades produced quite a number of biocontrol products that are already marketed. Some of the well-accepted commercial products contain bacteria Bacillus firmus and Pasteuria penetrans, and fungus Purpureocillium lilacinus. In this review we summarize the antagonistic activity of bacteria and fungi, with their advantages and limitations in biocontrol of root-knot nematodes.
Walnut husk fly (Rhagoletis completa) is an economically important fruit fly, which attacks several species of walnuts (Juglans spp). The insect is indigenous to North America, the best method for ...trapping the walnut husk fly adults is a yellow sticky board with ammonium carbonate as an attractant. In the present paper the bionomics, geographical distribution, methods of monitoring and controlling the walnut husk fly with special emphasis on biological control of the pest are presented. In a scientific literature is a lack of information regarding biological control, however if we take into consideration the foreign researches, Slovenian legislation and our experiences we suggest for biological control of walnut husk fly the foliar application of entomopathogenic fungi Beauveria bassiana against adults, soil application of entomopathogenic nematodes against larvae in autumn and spring soil application of entomopathogenic nematodes against adults, when they emerge from pupas. Monitoring of domestic parasitoids of walnut husk fly will be in the future needed in areas where the walnuts are expanded. On different areas of the world several species of parasitoids (Coptera occidentalis, Diachasmimorpha juglandis) are mentioned as an alternative biological control agents to chemicals.
In preceding researches on occurrence of entomopathogenic nematodes in Slovenia, which started in 2007, we already recorded Steinernema affine (Bovien), S. feltiae (Filipjev) and S. carpocapsae ...(Weiser). In April 2008, 120 soil samples from 24 locations were collected in Gorenjska, Notranjska and Primorska regions as well as in Ljubljansko barje. The presence of entomopathogenic nematodes was confirmed in 9 samples from 6 locations. Only the sample C46, which was taken in the village Podbrezje in Gorenjska region, was sent to genetic analysis. Molecular biological analysis have proved the identity of the sample with the species Steinernema kraussei (Steiner). This was the first record of Steinernema kraussei in Slovenia.
V predhodnih raziskavah preučevanja razširjenosti entomopatogenih ogorčic v Sloveniji, ki potekajo od leta 2007, smo ugotovili zastopanost vrst Steinernema affine (Bovien), Steinernema feltiae (Filipjev) in Steinernema carpocapsae (Weiser). V aprilu 2008 smo na območjih Gorenjske, Notranjske, Primorske in Ljubljanskega barja na 24 lokacijah nabrali 120 talnih vzorcev. Zastopanost entomopatogenih ogorčic smo ugotovili v 9 vzorcih z 6 lokacij. V nadaljnjo genetsko analizo smo poslali le vzorec C46. Ta je bil odvzet v vasi Podbrezje na Gorenjskem. Z molekulsko analizo smo identificirali vrsto Steinernema kraussei (Steiner). Gre za prvo odkritje omenjene vrste entomopatogene ogorčice pri nas.
In April 2008, 120 soil samples from 24 locations were collected in Gorenjska, Notranjska and Primorska regions as well as in Ljubljansko barje. The presence of entomopathogenic nematodes was ...confirmed in 9 samples from 6 locations. Only the sample C101, which was taken in the village Svino in the area of Breginjski kot (western part of Slovenia, the vicinity of Italian border), was sent to genetic analysis. Molecular biological analysis was proved the identity of the sample with the species Steinernema carpocapsae (Weiser). This was the first record of Steinernema carpocapsae in Slovenia. In preceding researches on the fauna of entomopathogenic nematodes in Slovenia, which started in 2007, we already established the occurrence of Steinernema affine (Bovien) and Steinernema feltiae (Filipjev).
V aprilu 2008 smo na območju Gorenjske, Notranjske, Primorske in Ljubljanskega barja na 24 lokacijah nabrali 120 talnih vzorcev. Zastopanost entomopatogenih ogorčic smo ugotovili v 9 vzorcih s 6 lokacij. V nadaljnjo genetsko analizo smo poslali le vzorec C101. Ta je bil odvzet v vasi Svino na območju Breginjskega kota (skrajni zahod Slovenije na meji z Italijo). Z molekulsko analizo smo identificirali vrsto Steinernema carpocapsae (Weiser). Gre za prvo odkritje omenjene vrste entomopatogene ogorčice pri nas. V predhodnjih raziskavah preučevanja faune entomopatogenih ogorčic v Sloveniji, ki potekajo od leta 2007, smo ugotovili zastopanost vrst Steinernema affine (Bovien) in Steinernema feltiae (Filipjev).
In a laboratory experiment an efficacy of entomopathogenic nematode Steinernema feltiae in controlling third-stage larvae of common cockchafer (Melolontha melolontha) was studied. The experiment ...comprised of commercial product Entonem and indigenous strain C76. The efficacy of both biological agents was tested at 20 and 25 °C and at four different concentrations of nematode suspension: 0, 250.000 infective juveniles IJs/m2, 500.000 IJs/m2, and 1.000.000 IJs/m2. Higher mortality rate (27 %) of white grubs was obtained for strain C76 rather than for commercial product (20 %). In our experiment temperature proved to be the most limiting factor in efficacy of tested biological agents. Meanwhile, mortality rate at 20 °C was 34 % and only 12 % mortality was achieved at 25 °C. At highest concentration of nematode suspension and 20 °C also the highest mortality rate (53 %) with strain C76 was obtained.
V laboratorijskem poskusu smo preučevali učinkovitost entomopatogene ogorčice Steinernema feltiae za zatiranje ličink tretje larvalne stopnje poljskega majskega hrošča (Melolontha melolontha). V poskus smo vključili komercialni pripravek Entonem in domorodno raso C76. Delovanje omenjenih biotičnih agensov smo ugotavljali pri 20 in 25 °C ter štirih različnih koncentracijah suspenzije ogorčic: 0, 250.000 infektivnih ličink IL/m2, 500.000 IL/m2 in 1.000.000 IL/m2. Rasa C76 je vplivala na višjo stopnjo smrtnosti (27 %) ogrcev, v primerjavi s komercialnim pripravkom (20 %). Temperatura se je v našem poskusu izkazala kot najbolj omejujoč dejavnik učinkovitosti preizkušanih biotičnih agensov, saj smo pri 20 °C dosegli 34 % smrtnost ogrcev, medtem ko je bila ta pri 25 °C le 12 %. Pri najvišji koncentraciji suspenzije ogorčic in 20 °C je bila pri rasi C76 dosežena najvišja stopnja smrtnosti ogrcev, in sicer 53 %.
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