Species relationships in the bottlenose dolphin (genus Tursiops Gervais, 1855) are controversial. We carried out a comprehensive osteological study of 264 skulls, including type specimens, and 90 ...postcranial skeletons of Tursiops spp. to address taxonomic uncertainties in Australia using two-dimensional (2D) measurements, and three-dimensional geometric morphometrics (3DGM), tooth and vertebral counts, and categorical data. Analyses provided support for the presence of two forms, aligned to the Indo-Pacific bottlenose dolphin (Tursiops aduncus (Ehrenberg, 1832)) and the common bottlenose dolphin (Tursiops truncatus (Montagu, 1821)), including type specimens. The Burrunan dolphin (Tursiops australis Charlton-Robb, Gershwin, Thompson, Austin, Owen and McKechnie, 2011) fell well within T. truncatus for both 2D and 3DGM methods. Thirteen Tursiops spp. specimens, no T. australis specimens, were of intermediate size (2D) and could not be assigned to either species. For 3DGM data, there was a strong allometric influence and few non-allometric differences between species. Length and width of the cranium and rostrum were important discriminating variables. Tursiops aduncus was smaller, had more teeth, fewer vertebrae, and more erosion on the pterygoids and frontals than T. truncatus. Overall cranium shape was round in T. aduncus and angular in T. truncatus. Skull length of T. aduncus was smaller in low than in high latitudes. This study highlights the importance of large sample size, multiple analytical methods, and extensive geographical coverage when undertaking taxonomic studies.
Recreational boats are a dominant source of underwater noise in coastal areas, but reliable boat noise assessment is generally lacking. Here the Underwater Radiated Noise (URN) of seven recreational ...and small fishing boats moving at two different speeds was measured in the shallow waters of the Cres-Lošinj Natura 2000 SCI (Croatia). Measurements were undertaken considering the internationally recognized standards and published guidelines for shallow waters. URN was provided in 1/3 octave band spectra and in narrow band spectra, to highlight the tonal components. Engine power and type rather than the boat length and design result to be more predictive of URN. Highest speeds induce highest noise levels only in a very limited frequency range and different boats with similar speed but different engines show a shift in the spectra. Relevance of the achieved results for the tested area is further discussed.
•Underwater Radiated Noise of seven boat types was measured in a shallow environment.•Underwater Radiated Noise was provided as 1/3 octave and narrow band spectra.•The spectra show strong tonal components.•Boats with similar speed but different engines show different noise emissions.•Highest speed induces highest noise levels only in a very limited frequency range.
In this study, we examined the usefulness of a simpler and more feasible method for determining the optimal timing of artificial insemination and the conditions for its success in six female common ...bottlenose dolphins. Pregnancy was successfully achieved in five dolphins by performing intrauterine insemination, using chilled semen stored for less than 3 days or frozen semen within 24 hr of exhibiting a peak serum estradiol (E2) level of 100 pg/mL or higher or on the day with a serum E2 level of approximately 100 pg/mL, measured with a simple measuring device. We concluded that the determining the optimal timing of intrauterine insemination by measuring serum E2 levels is a simpler and more useful method compared with the conventional approach.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants. Although they are not bioaccumulated in vertebrates, chronic exposures might still derive on serious toxic effects. We ...studied concentrations of 16 reference PAHs on blubber of two dolphin species (striped dolphin, n = 34; and bottlenose dolphin, n = 8) and one marine turtle (loggerhead turtle, n = 23) from the Mediterranean waters of SE Spain, an important or potential breeding area for these and other related species. Σ16 PAHs concentrations were relatively similar between the three species, but they were in the lower range in comparison to worldwide data. Of the six PAHs detected, fluoranthene was the only high molecular weight (HMW) PAH, so low molecular weight (LMW) PAHs predominated. Naphthalene and phenanthrene were invariably those PAHs with higher detection rates as well as those with higher concentrations. In accordance with the literature, sex and length did not have significant influence on PAHs concentrations, probably due to high metabolization rates which prevent for observation of such patterns. Despite LMW PAHs are considered less toxic, we cannot dismiss toxic effects. This is the first work assessing PAHs concentrations in cetaceans and sea turtles from the SE Spain, which could serve as the baseline for future research.
Display omitted
•16 PAHs were measured in fatty tissues from Mediterranean dolphins and marine turtles.•Detectable concentrations of several PAHs are reported in marine top predators.•The constant detection of the easily excretable PAHs demonstrates a constant exposure.•The tissue pattern of PAHs suggests eminently a petrogenic origin.
The excretion of tapeworm strobilla from the anus of a common bottlenose dolphin caught off the Kii Peninsula was observed. Based on the arrangement of the reproductive organs, it was assumed to ...belong to the genus Diphyllobothrium Cobbold, 1858 (the family Diphyllobothriidae). In addition, molecular diagnosis based on the mitochondrial COX-1, the ribosomal ITS1, and 18S rRNA gene was performed. As a result of the morphological observation and molecular analyses, the species was identified as Diphyllobothrium stemmacephalum. This is the first report of the detection of Diphyllobothrium stemmacephalum in a bottlenose dolphin in Japan.
Per-and poly-fluorinated alkyl substances (PFAS) are a group of chemicals used in a wide variety of commercial products and industrial applications. These chemicals are persistent, can accumulate in ...humans' and animals' tissues and in the environment, representing an increasing concern due to their moderate to highly toxicity. Their global distribution, persistence and toxicity led to an urgent need to investigate bioaccumulation also in marine species. In 2013 PFAS contamination was detected in a vast area in Veneto region, mainly in Adige and Brenta rivers. In order to investigate any relevant presence of these substances in marine vertebrates constantly living in the area, PFAS were measured in hepatic tissue samples of 20 bottlenose dolphins (Tursiops truncatus) stranded along the northern Adriatic Sea coastline between 2008 and 2020. Using high performance liquid chromatography-mass spectrometry, 17 target PFAS (PFBA, PFPeA, PFHxA, PFHpA, PFOA, PFNA, PFDA, PFUnA, PFDoA, PFTrDA, PFTeDA, PFBS, PFHxS, PFOS, PFDS, PFHpS, PFPeS), were quantified in the samples. PFAS profiles were generally composed of the same five dominant PFAS (PFOS > PFUnA > PFDA ≈ PFDoA ≈ PFTrDA). The greatest PFOS concentration found was 629,73 ng/g wet weight, and PFOS accounted until 71% in the PFAS profiles. No significant differences between sexes were found, while calves showing higher mean values than adults, possibly indicating an increasing ability in the elimination of PFAS with age. Finally, a temporal analysis was carried out considering three different periods of time, but no temporal differences in concentrations were found. The results suggest that long-chain PFAS are widespread in bottlenose dolphins along the North Adriatic Sea. Furthermore, they represent a baseline to investigate the impact of PFAS on marine mammals’ conservation and health. Filling an important gap in the knowledge of PFAS accumulation in bottlenose dolphins, this study highlights the relevant role of Environmental and Tissue Banks for retrospective analyses on emergent contaminants.
Photobacterium damselae subspecies damselae, an abundant, generalist marine pathogen, has been reported in various cetaceans worldwide. We report a bottlenose dolphin in the eastern Mediterranean Sea ...that was found stranded and dead. The dolphin had a severe case of chronic suppurative pneumonia and splenic lymphoid depletion caused by this pathogen.
We characterized novel coronaviruses detected in US bottlenose dolphins (BdCoVs) with diarrhea. These viruses are closely related to the other 2 known cetacean coronaviruses, Hong Kong BdCoV and ...beluga whale CoV. A deletion in the spike gene and insertions in the membrane gene and untranslated regions were found in US BdCoVs (unrelated to severe acute respiratory syndrome coronavirus 2).
Few accounts describe predator-prey interactions between common bottlenose dolphins (Tursiops truncatus Montagu 1821) and marine catfish (Ariopsis felis Linnaeus 1766, Bagre marinus Mitchill 1815). ...Over the course of 50,167 sightings of bottlenose dolphin groups in Mississippi Sound and along the Florida coast of the Gulf of Mexico, severed catfish heads were found floating and exhibiting movements at the surface in close proximity to 13 dolphin groups that demonstrated feeding behavior. These observations prompted a multi-disciplinary approach to study the predator-prey relationship between bottlenose dolphins and marine catfish. A review was conducted of bottlenose dolphin visual survey data and dorsal fin photographs from sightings where severed catfish heads were observed. Recovered severed catfish heads were preserved and studied, whole marine catfish were collected and examined, and stranding network pathology reports were reviewed for references to injuries related to fish spines. Photographic identification analysis confirms eight dolphins associated with severed catfish heads were present in three such sightings across an approximately 350 km expanse of coast between the Mississippi Sound and Saint Joseph Bay, FL. An examination of the severed catfish heads indicated interaction with dolphins, and fresh-caught whole hardhead catfish (A. felis) were examined to estimate the presumed total length of the catfish before decapitation. Thirty-eight instances of significant trauma or death in dolphins attributed to ingesting whole marine catfish were documented in stranding records collected from the southeastern United States of America. Bottlenose dolphins typically adhere to a ram-feeding strategy for prey capture followed by whole prey ingestion; however, marine catfish skull morphology may pose a consumption hazard due to rigid spines that can puncture and migrate through soft tissue, prompting a prey handling technique for certain dolphins, facilitating consumption of the posterior portion of the fish without the head.
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), considered a zoonotic agent of wildlife origin, can infect various animal species, including wildlife in free-range and captive ...environments. Detecting susceptible species and potential reservoirs is crucial for preventing the transmission, spread, genetic evolution, and further emergence of viral variants that are major threats to global health. This study aimed to detect exposure or acute infection by SARS-CoV-2 in 420 animals from 40 different wildlife species, including terrestrial and aquatic mammals, from different regions of Spain during the 2020–2023 coronavirus disease 19 (COVID-19) pandemic. In total, 8/137 animals were positive for SARS-CoV-2 antibodies against the receptor binding domain and/or viral nucleoprotein according to independent ELISAs. However, only one ELISA-positive sample of a captive bottlenose dolphin ( Tursiops truncatus ) tested positive for SARS-CoV-2 neutralizing antibodies with a low titre (SNT 50 38.15) according to a virus neutralization test. Cetaceans are expected to have a high risk of infection with SARS-CoV-2 according to early predictive studies due to the similarity of their angiotensin converting enzyme 2 cell receptor to that of humans. Moreover, of 283 animals analysed for SARS-CoV-2 RNA using RT-qPCR, none tested positive. Our results reinforce the importance of considering cetaceans at risk for SARS-CoV-2 infection and support taking preventive biosecurity measures when interacting with them, especially in the presence of individuals with suspected or confirmed COVID-19. Although most animals in this study tested negative for acute infection or viral exposure, ongoing surveillance of wildlife species and potentially susceptible animals is important to prevent future spillover events and detect potential novel reservoirs.
PDF
