Seven commercial hybrids of Phalaenopsis were included in an asymbiotic germination experiment. Plants differed in size and color of flowers and number of inflorescences and flowers. A total of 109 ...flowers were included of which 60 or 55% were cross-pollinated and reciprocally pollinated. Seed capsules developed on 46 (76.7%) fertilized flowers. Of these, 38 (82.6%) produced seeds as a result of 19 combinations of cross crossing and 19 combinations of reciprocal crossing. Seeds were surface sterilized using 1.6% dichloroisocyanuric acid and inoculated in Petri dishes on commercial media Sigma P1056, in two repeats. There were 23.7% contaminated Petri dishes with seeds after sterilization. The highest average percentage of protocorms (43.1%) developed in cross crossing of plants 3×7, which statistically significantly differed (p < 0.001) from all other combinations of cross crossing except cross crossing 2×6 (30.6%). Crossing 2×6, 1×6 (8.1%) and 1×5 (5.5%) overlap and there were no statistically significant differences between them. There were no statistically significant differences among all of the remaining crossings. There was an overlap of groups among combinations of cross crossing with small flowers x big flowers and reciprocal crossing of big flowers x small flowers. In successful crossing combinations, first plants with two leaves and one or two roots developed only 80 days after seed inoculation on media. Plant no. 4 with the smallest green-yellow flowers was not compatible with any test plants. Seed capsules without seeds developed when plant 4 was a female plant and flowers just fell off when plant 4 was a male plant.
V poskus asimbiotske kalitve je bilo vključenih 7 komercialnih orhidej iz rodu Phalaenopsis, ki so se razlikovale po velikosti in barvi cvetov ter številu socvetij in cvetov. Skupno je bilo na njih 109 cvetov, od teh je bilo 60 oz. 55% navzkrižno in recipročno oprašenih. Semenske glavice je oblikovalo 46 oz. 76,7% oplojenih cvetov. V 38 oz. v 82,6% semenskih glavicah so nastala semena, kot rezultat 19 kombinacij navzkrižnega in 19 kombinacij recipročnega križanja. Semena so bila površinsko razkužena z 1,6% dikloroizocianurno kislino in inokulirana v dveh ponovitvah na komercialno gojišče Sigma P1056 v petrijevke. Po razkuževanju je bilo okuženih 23,7% petrijevk s semeni. Največji odstotek nastalih protokormov (43,1%) je bil dobljen pri navzkrižnem križanju rastlin 3×7, ta odstotek se statistično značilno (p < 0.001) razlikuje od ostalih kombinacij navzkrižnih križanj, razen navzkrižnega križanja 2×6 (30,6%). Križanja 2×6, 1×6 (8.1%) in 1×5 (5.5%) se prekrivajo in med njimi ni statistično značilnih razlik. Prav tako ni statistično značilnih razlik med ostalimi kombinacijami križanja. Med skupinami navzkrižnih križanj rastlin z malimi cvetovi x rastlin z večjimi cvetovi in recipročnih križanj rastlin z velikimi cvetovi x malimi cvetovi pride do medsebojnega prekrivanja in med njimi ni statistično značilnih razlik. Pri uspešnih kombinacijah križanj so že po 80 dneh inokulacije semen na gojišče nastajale prve rastline z dvema listoma in eno ali dvema koreninama. Rastlina z oznako 4 z najmanjšimi, zeleno rumenimi cvetovi ni bila kompatibilna z nobeno od vključenih rastlin v križanja. V primerih, ko je bila materina rastlina, so nastale semenske glavice brez semen, v primerih, ko je bila očetova rastlina, so cvetovi po oprašitvi odpadli.
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