Developing stable, robust, and affordable tissue-mimicking phantoms is a prerequisite for any new clinical application within biomedical optics. To this end, a thorough understanding of the phantom ...structure and optical properties is paramount.
We characterized the structural and optical properties of PlatSil SiliGlass phantoms using experimental and numerical approaches to examine the effects of phantom microstructure on their overall optical properties.
We employed scanning electron microscope (SEM), hyperspectral imaging (HSI), and spectroscopy in combination with Mie theory modeling and inverse Monte Carlo to investigate the relationship between phantom constituent and overall phantom optical properties.
SEM revealed that microspheres had a broad range of sizes with average
and were also aggregated, which may affect overall optical properties and warrants careful preparation to minimize these effects. Spectroscopy was used to measure pigment and SiliGlass absorption coefficient in the VIS-NIR range. Size distribution was used to calculate scattering coefficients and observe the impact of phantom microstructure on scattering properties. The results were surmised in an inverse problem solution that enabled absolute determination of component volume fractions that agree with values obtained during preparation and explained experimentally observed spectral features. HSI microscopy revealed pronounced single-scattering effects that agree with single-scattering events.
We show that knowledge of phantom microstructure enables absolute measurements of phantom constitution without prior calibration. Further, we show a connection across different length scales where knowledge of precise phantom component constitution can help understand macroscopically observable optical properties.
In the above article <xref ref-type="bibr" rid="ref1">1 , <xref rid="fig1" ref-type="fig">Fig. 19 was incorrectly placed. The correct image and caption are provided here:
The feasibility of combining spectral and textural information from hyperspectral imaging to improve the prediction of the C16:0 and C18:1 n9 contents for lamb was explored. 29 and 22 optimal ...wavelengths were selected for the C16:0 and C18:1 n9 contents, respectively, by conducting the variable combination population analysis–iteratively retaining informative variables (VCPA-IRIV) algorithm. To extract the textural features of images, a gray-level co-occurrence matrix (GLCM) analysis was implemented in the first principal component image. The least squares support vector machine (LSSVM) model and the partial least squares regression (PLSR) model were developed to predict the C16:0 and C18:1 n9 contents from the spectra and the fusion data. The distribution map was visualized using the best model with the imaging process. The results showed that the combination of the spectral and textural information of hyperspectral imaging coupled with the VCPA-IRIV algorithm had strong potential for the prediction and visualization of the C16:0 and C18:1 n9 contents of lamb.
The evaluation of tea quality tended to be subjective and empirical by human panel tests currently. A convenient analytical approach without human involvement was developed for the quality assessment ...of tea with great significance. In this study, near-infrared hyperspectral imaging (HSI) combined with multiple decision tree methods was utilized as an objective analysis tool for delineating black tea quality and rank. Data fusion that integrated texture features based on gray-level co-occurrence matrix (GLCM) and short-wave near-infrared spectral features were as the target characteristic information for modeling. Three different types of supervised decision tree algorithms (fine tree, medium tree, and coarse tree) were proposed for the comparison of the modeling effect. The results indicated that the performance of models was enhanced by the multiple perception feature fusion. The fine tree model based on data fusion obtained the best predictive performance, and the correct classification rate (CCR) of evaluating black tea quality was 93.13% in the prediction process. This work demonstrated that HSI coupled with intelligence algorithms as a rapid and effective strategy could be successfully applied to accurately identify the rank quality of black tea.
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•700 black tea samples encompassing seven classes are delineated by HSI.•Description models built using multiple decision tree methods.•NIR and image feature fusion to identify black tea tenderness and classes.•FT model employing fusion eigenvectors obtain the best predictive results.
Consumption of poultry products is increasing worldwide, leading to an increased demand for safe, fresh, high-quality products. To ensure consumer safety and meet quality standards, poultry products ...must be routinely checked for fecal matter, food fraud, microbiological contamination, physical defects, and product quality. However, traditional screening methods are insufficient in providing real-time, nondestructive, chemical and spatial information about poultry products. Novel techniques, such as hyperspectral imaging (HSI), are being developed to acquire real-time chemical and spatial information about products without destruction of samples to ensure safety of products and prevent economic losses. This literature review provides a comprehensive overview of HSI applications to poultry products. The studies used for this review were found using the Google Scholar database by searching the following terms and their synonyms: “poultry” and “hyperspectral imaging”. A total of 67 studies were found to meet the criteria. After all relevant literature was compiled, studies were grouped into categories based on the specific material or characteristic of interest to be detected, identified, predicted, or quantified by HSI. Studies were found for each of the following categories: food fraud, fecal matter detection, microbiological contamination, physical defects, and product quality. Key findings and technological advancements were briefly summarized and presented for each category. Since the first application to poultry products 20 yr ago, HSI has been shown to be a successful alternative to traditional screening methods.
The structural features of precooked noodles during refrigerated storage were non-destructively characterized using hyperspectral imaging (HSI) technology along with conventional analytical methods. ...The precooked noodles displayed a more rigid texture and restricted water mobility over the storage period, derived from the recrystallization of starch. Dimensionality reduction techniques revealed robust correlations between the storage duration and HSI absorbance of the noodles, and from their loading plots, the specific peaks of the noodles related to their structural changes were identified at wavelengths of around 1160 and 1400 nm. The strong relationships between the HSI results of the noodles and their storage period/texture were confirmed by training four machine learning models on the HSI data. In particular, the support vector algorithm displayed the best prediction performance for classifying precooked noodles by storage period (98.3% accuracy) and for predicting the noodle texture (R2 = 0.914).
•Precooked noodle structure during refrigeration was assessed by machine learning-HSI.•Dimensionality reduction of HIS data categorized noodles by refrigerated storage time.•Hyperspectral peaks at 1160/1400 nm were related to the structural changes.•SVM model successfully identified the storage period of noodles (accuracy = 0.983).•SVR showed the great performance in predicting a noodle texture (R2=0.914).
Microplastic pollution is a global concern theme, and there is still the need for less laborious and faster analytical methods aiming at microplastics detection. This article describes a high ...throughput screening method based on near-infrared hyperspectral imaging (HSI-NIR) to identify microplastics in beach sand automatically with minimum sample preparation. The method operates directly in the entire sample or on its retained fraction (150 μm–5 mm) after sieving. Small colorless microplastics (<600 μm) that would probably be imperceptible as a microplastic by visual inspection, or missed during manual pick up, can be easily detected. No spectroscopic subsampling was performed due to the high-speed analysis of line-scan instrumentation, allowing multiple microplastics to be assessed simultaneously (video available). This characteristic is an advantage over conventional infrared (IR) spectrometers. A 75 cm2 scan area was probed in less than 1 min at a pixel size of 156 × 156 μm. An in-house comprehensive spectral dataset, including weathered microplastics, was used to build multivariate supervised soft independent modelling of class analogy (SIMCA) classification models. The chemometric models were validated for hundreds of microplastics (primary and secondary) collected in the environment. The effect of particle size, color and weathering are discussed. Models' sensitivity and specificity for polyethylene (PE), polypropylene (PP), polyamide-6 (PA), polyethylene terephthalate (PET) and polystyrene (PS) were over 99% at the defined statistical threshold. The method was applied to a sand sample, identifying 803 particles without prior visual sorting, showing automatic identification was robust and reliable even for weathered microplastics analyzed together with other matrix constituents. The HSI-NIR-SIMCA described is also applicable for microplastics extracted from other matrices after sample preparation. The HSI-NIR principals were compared to other common techniques used to microplastic chemical characterization. The results show the potential to use HSI-NIR combined with classification models as a comprehensive microplastic-type characterization screening.
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•HSI-NIR saves time with minimum sample preparation for microplastic identification.•Hundreds of microplastics were rapidly and simultaneously identified using chemometrics.•A comprehensive microplastic spectral library was built for modelling purposes.•SIMCA classification models validated for five polymers (PE, PP, PS, PA-6 and PET).•Potential method to be used as a fast screening in microplastic analysis.
Near Infrared Hyperspectral Imaging (HSI-NIR) and the chemometric models developed were successfully applied to multiple, fast and automatic microplastic polymer identification.
In the above article <xref ref-type="bibr" rid="ref1">1 , it should be noted that the second value of each column in the last row of <xref rid="table1" ref-type="table">Table I (i.e., 0.52%, 0.35%, ...0.51%, and 0.72%) is calculated using average deviation rather than standard deviation. In order to be consistent with the title of <xref rid="table1" ref-type="table">Table I in <xref ref-type="bibr" rid="ref1">1 , the corresponding standard deviations are provided here.
Convolutional neural networks (CNNs) exhibit good performance in image processing tasks, pointing themselves as the current state-of-the-art of deep learning methods. However, the intrinsic ...complexity of remotely sensed hyperspectral images still limits the performance of many CNN models. The high dimensionality of the HSI data, together with the underlying redundancy and noise, often makes the standard CNN approaches unable to generalize discriminative spectral-spatial features. Moreover, deeper CNN architectures also find challenges when additional layers are added, which hampers the network convergence and produces low classification accuracies. In order to mitigate these issues, this paper presents a new deep CNN architecture specially designed for the HSI data. Our new model pursues to improve the spectral-spatial features uncovered by the convolutional filters of the network. Specifically, the proposed residual-based approach gradually increases the feature map dimension at all convolutional layers, grouped in pyramidal bottleneck residual blocks, in order to involve more locations as the network depth increases while balancing the workload among all units, preserving the time complexity per layer. It can be seen as a pyramid, where the deeper the blocks, the more feature maps can be extracted. Therefore, the diversity of high-level spectral-spatial attributes can be gradually increased across layers to enhance the performance of the proposed network with the HSI data. Our experiments, conducted using four well-known HSI data sets and 10 different classification techniques, reveal that our newly developed HSI pyramidal residual model is able to provide competitive advantages (in terms of both classification accuracy and computational time) over the state-of-the-art HSI classification methods
This study aimed to develop simplified models for rapid and nondestructive monitoring myoglobin contents (DeoMb, MbO2 and MetMb) during refrigerated storage of Tan sheep based on a hyperspectral ...imaging (HSI) system in the spectral range of 400–1000 nm. Partial least squares regression (PLSR) and least-squares support vector machines (LSSVM) were applied to correlate the spectral data with the reference values of myoglobin contents measured by a traditional method. In order to simplify the LSSVM models, competitive adaptive reweighted sampling (CARS) and Interval variable iterative space shrinkage approach (iVISSA) were used to select key wavelengths. The new CARS-LSSVM models of DeoMb and MbO2 yielded good results, with R2p of 0.810 and 0.914, RMSEP of 1.127 and 2.598, respectively. The best model of MetMb was new iVISSA-CARS-LSSVM, with an R2p of 0.915 and RMSEP of 2.777. The overall results from this study indicated that it was feasible to predict myoglobin contents in Tan sheep using HSI.
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