Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research. Proteomics offers one of the best options for the functional analysis of translated regions of ...the genome, generating a wealth of detailed information regarding the intrinsic mechanisms of plant stress responses. Various proteomic approaches are being exploited extensively for elucidating master regulator proteins which play key roles in stress perception and signaling, and these approaches largely involve gel-based and gel-free techniques, including both label-based and label-free protein quantification. Furthermore, post-translational modifications, subcellular localization, and protein–protein interactions provide deeper insight into protein molecular function. Their diverse applications contribute to the revelation of new insights into plant molecular responses to various biotic and abiotic stressors.
DOI: 10.1002/pmic.201600209 Judicious use of detergents may reconcile the outstanding proteomic crisis of membrane protein analysis. The simplicity of detergent tools allows ultradeep coverage, ...minimal artefacts and robust measurement of membrane proteins and endogenous PTMs, and affords proteomics the sophistication toward human subtype-precise medicine and therapeutics. Emerging membrane proteomics evidence suggests PTMs confer the human subtype precision in human alpha1beta3gamma2L GABA type A receptor beyond the generic homomeric model, and this principle might be applicable to the various excitary and inhibitory neurotransmitter receptors in the brain as depicted by the bottom right image (3D structural images created from PDB files: 3RHW and 2QC1). For more details, see the review by Xi Zhang, article number 1600209.