To study the possible impact of astaxanthin on the cytogenetic manifestations of the simultaneous development of radiation-induced (RIBE) and tumor-induced bystander effect (TIBE) in human peripheral ...blood lymphocytes.
Peripheral blood lymphocytes from healthy persons and patients with chronic lymphocytic leukemia were cultured separately or cocultured with or without previous irradiation in vitro by
Cs at a dose of 0.5 Gy. The cells were cultured with and without 20 μg/ml astaxanthin.
In the presence of astaxanthin, the decrease of chromosomal instability both in the variant with separate TIBE and with simultaneous development of TIBE and RIBE was observed as the reduction in the frequency of simple chromosome-type aberrations, namely, double fragments. The average level of chromatid-type aberrations did not change under the action of astaxanthin. Although the total chromosome instability in bystander cells diminished, this did not lead to the elimination of the RIBE and TIBE development in the presence of astaxanthin.
In the setting of experiment, astaxanthin did not reduce the frequency of chromatid-type aberrations in bystander cells due to RIBE and TIBE but reduced the frequency of simple aberrations of chromosomal type, not associated with the development of bystander response phenomenon.
Sparebøsser fra middelalderens Ribe Søvsø, Mette Højmark
By, marsk og geest - Kulturhistorisk tidsskrift for Sydvestjylland,
06/2022
Journal Article
Odprti dostop
I Ribes middelalderlag findes jævnligt større og mindre fragmenter af sparebøsser af keramik. Ved samlingsgennemgangeni forbindelse med indflytningen i det nye magasin dukker desuden jævnligt ...hidtilupåagtede skår af sparebøsser op, og Sydvestjyske Museer rummer efterhånden en hel lille samling afdenne genstandsgruppe. I det følgende præsenteres de sparebøsser og skår, som indtil nu er identificereti museets samling.
Studies have been conducted at synchrotron facilities in Europe and Australia to explore a variety of applications of synchrotron X-rays in medicine and biology. We discuss the major technical ...aspects of the synchrotron irradiation setups, paying specific attention to the Australian Synchrotron (AS) and the European Synchrotron Radiation Facility (ESRF) as those best configured for a wide range of biomedical research involving animals and future cancer patients. Due to ultra-high dose rates, treatment doses can be delivered within milliseconds, abiding by FLASH radiotherapy principles. In addition, a homogeneous radiation field can be spatially fractionated into a geometric pattern called microbeam radiotherapy (MRT); a coplanar array of thin beams of microscopic dimensions. Both are clinically promising radiotherapy modalities because they trigger a cascade of biological effects that improve tumor control, while increasing normal tissue tolerance compared to conventional radiation. Synchrotrons can deliver high doses to a very small volume with low beam divergence, thus facilitating the study of non-targeted effects of these novel radiation modalities in both in-vitro and in-vivo models. Non-targeted radiation effects studied at the AS and ESRF include monitoring cell–cell communication after partial irradiation of a cell population (radiation-induced bystander effect, RIBE), the response of tissues outside the irradiated field (radiation-induced abscopal effect, RIAE), and the influence of irradiated animals on non-irradiated ones in close proximity (inter-animal RIBE). Here we provide a summary of these experiments and perspectives on their implications for non-targeted effects in biomedical fields.
Experimental results on the application of reflectance anisotropy spectroscopy (RAS) to the monitoring of (reactive) ion etching of monocrystalline semiconductor samples are described. To show the ...potential of this technique RAS signals collected during etching of GaAs/AlxGa1−xAs multilayer samples are compared to RAS data obtained before during molecular-beam epitaxial (MBE) growth of these very samples. A change of the RIE-RAS spectrum can be attributed to a change of material composition. And the current etch depth can be monitored with an accuracy at least down to several tens of nanometers – f. e. by recording the average reflected intensity.
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