The levels of soluble c-kit ligand (sKL), also known as Steel factor or stem cell factor, were measured in blood serum and long-term bone marrow culture supernatants of 81 patients with chronic ...idiopathic neutropenia (CIN) and 22 normal controls using a commercially available enzyme-linked immunosorbent assay (ELISA). We found that the levels of serum and culture supernatant sKL did not differ significantly between patients and control subjects and that both serum and supernatant values of the cytokine did not correlate with the number of circulating neutrophils. Furthermore, we found that the levels of the culture supernatant granulocyte colony-stimulating factor (G-CSF), also measured by ELISA, were significantly increased in the patients compared to controls but individual G-CSF values did not correlate with the values of supernatant sKL. These findings suggest that sKL-producing cells continuously secrete sKL and that cytokine secretion is independent of the degree of neutropenia or the levels of supernatant G-CSF in patients with CIN.
This article examines the effects of test format and task type on candidate output in direct and semidirect versions of the oral interaction subtest of the Australian Assessment of Communicative ...English Skills. Results are discussed in relation to the degree of interactiveness and other factors that appear to influence lexical density and to the validity and interchangeability of the two kinds of oral proficiency tests. (JL)
The question was addressed whether antibodies directed against the general microbody C-terminal protein targeting signal SKL recognized hydrogenosomal proteins from Neocallimastix sp. L2. ...Immunofluorescence, immunocytochemistry and Western blotting experiments using these antibodies indicated the presence of hydrogenosomal proteins containing SKL-COOH. One of these proteins, the hydrogenase, was purified to homogeneity. It has a native molecular mass of 87 kDa and consists of two subunits of approximately 30 and 60 kDa, both cross-reacting with anti-SKL antibodies. Its activity could be inhibited by CO, NO2-, and acetylene, suggesting a (Ni-Fe-Se) hydrogenase. Immunocytochemistry using polyclonal antibodies raised against the hydrogenase revealed the location of this protein in the hydrogenosomal matrix. The results described in this paper suggest that hydrogenosomes from Neocallimastix sp. L2 are related to microbodies from aerobic eukaryotes and support the idea of a common evolutionary origin for these organelles.
In this paper, a new adaptive subspace learning model based on incremental nonparametric discriminant analysis (INDA) is proposed for visual tracking. Traditional subspace trackers focus on updating ...eigenvectors in handling with appearance variation of the target object, ignoring the non-target background region during tracking. The INDA features take both of them into consideration, thereby promoting the tracking process in the ever-changing environment. Meanwhile, INDA relaxes the Gaussian assumption in Fisher discriminant analysis (FDA), so it can handle more general class distributions problem. The scatter matrices are also reformulated to update the subspace incrementally based on previous results. In conjunction with efficient feature extraction method, the system is real time capable. Numerous experiments show the superiority of our tracker over current states of art methods on several publicly available datasets.
Utstedt av Jacob Kielland & Søn, år 1809
Jacob Kielland & Søn / 1809
For 48 Skilling Dansk Courant
I mod Anvisning betaler Handelshuset Jacob Kielland & Søn til Thendehaveren En halv Rixdaler Dansk ...Courant, hvorav Valuta bekommet Nordre Hummer Fiskevæhr Ao 1809 i Bergen hos...(sign) og i Stavanger av...(sign)
48 SKL. Antages til Betaling
Viral DNA of unintegrated closed circular form was isolated from a swine kidney cell line (SKL) which was infected with a porcine retrovirus Tsukuba-1 (PRetV) produced from a swine malignant ...lymphoma-derived cell line, Shimozuma-1 and cloned using a λ phage vector; Charon 21 A. One of ten independent clones contained the 8.3 kb DNA fragment as an insert, which was thought to be a full length of viral DNA molecule carrying a long terminal repeat (LTR) sequence. We have analyzed this insert by mapping the recognition sites of some restriction endonucleases by Southern blot hybridization with appropriate probes.
Proposes and explains a new mechanism for controlling the access requests in information protection systems that is based on Newton's interpolating polynomial. Access control matrices are discussed; ...single-key-lock systems are described; relevant algorithms are explained; and an example of the new mechanism is included. (10 references) (LRW)
Peroxisome, an ubiquitous subcellular organelle in eukaryotes, functions in many crucial pathways in metabolisms such as catabolism by beta-oxidation of very long chain fatty acids, biosynthesis of ...etherglycerolipids, and metabolism of cholesterol. To address the question how peroxisomes are assembled in eukaryotic cells, we discuss here two topics undertaken in our laboratory. Peroxisomes are formed by posttranslational assembly mechanism; peroxisomal proteins are synthesized on free polysomes in the cytosol, mostly at their final sizes. This implies that topogenic signal(s) for import of newly synthesized polypeptides into peroxisomes reside in the internal sequence of proteins. Peroxisome-targeting signal has been noted in vivo and in vitro for enzymes such as luciferase and acyl-CoA oxidase (AOX). The topogenic signal resides at the extreme C-terminus and comprises tripeptide-Ser-Lys-Leu-COOH (SKL). Further experiments have strongly suggested that the SKL motif, Ser/Ala-Lys/Arg/His-Leu-COOH commonly found at C-termini of many peroxisomal proteins, functions as a peroxisome-targeting signal. Among several human genetic peroxisomal disorders, cerebrohepatorenal syndrome (Zellweger syndrome) is a typical, severe disease with absence of peroxisome, where a peroxisome assembly is likely to be defective. We isolated three mutants (Z24, Z65, and ZP92), recessive to wild-type cell and mutually complementary, of Chinese hamster ovary (CHO) cells that resemble the fibroblasts from Zellweger patients. To investigate molecular mechanism of peroxisome assembly and primary defects of human peroxisome-deficient disorders, we searched for the genes encoding factors that complement dysfunctions of CHO cell mutants. The mutants transfected with a pcD2-rat liver cDNA library were selected in the presence of G418.(ABSTRACT TRUNCATED AT 250 WORDS)
