The enduring relationship between dietary patterns and human health has led us to investigate the bioactive components present in fruits and vegetables for a very long time. Berries, notably the ...popular ones such as strawberry, raspberry, blueberry, blackberry, and the Indian gooseberry, are among the best known dietary sources due to the presence of a wide range of bioactive nutritive components. Bioactive components in berries include phenolic compounds, flavonoids, and tannins apart from vitamins, minerals, sugars, and fibers. Individually or synergistically, these have been shown to provide protection against several disorders. Mounting evidence suggests that consumption of berries confer antioxidant and anticancer protection to humans and animals. Free radical scavenging, protection from DNA damage, induction of apoptosis, and inhibition of growth and proliferation of cancer cells are just to name a few. This review comprehensively summarizes the key phytochemicals present in berries and their biological action in preventing oxidative stress and carcinogenesis.
Summary
The RAV (related to ABI3/viviparous 1) group of transcription factors (TFs) play multifaceted roles in plant development and stress responses. Here, we show that strawberry ...(Fragaria × ananassa) FaRAV1 positively regulates anthocyanin accumulation during fruit ripening via a hierarchy of activation processes. Dual‐luciferase assay screening of all fruit‐expressed AP2/ERFs showed FaRAV1 had the highest transcriptional activation of the promoter of FaMYB10, a key activator of anthocyanin biosynthesis. Yeast one‐hybrid and electrophoretic mobility shift assays indicated that FaRAV1 could directly bind to the promoter of FaMYB10. Transient overexpression of FaRAV1 in strawberry fruit increased FaMYB10 expression and anthocyanin production significantly. Correspondingly, transient RNA interference‐induced silencing of FaRAV1 led to decreases in FaMYB10 expression and anthocyanin content. Transcriptome analysis of FaRAV1‐overexpressing strawberry fruit revealed that transcripts of phenylpropanoid and flavonoid biosynthesis pathway genes were up‐regulated. Luciferase assays showed that FaRAV1 could also activate the promoters of strawberry anthocyanin biosynthetic genes directly, revealing a second level of FaRAV1 action in promoting anthocyanin accumulation. These results show that FaRAV1 stimulates anthocyanin accumulation in strawberry both by direct activation of anthocyanin pathway gene promoters and by up‐regulation of FaMYB10, which also positively regulates these genes.
Strawberry (
spp.) has emerged as a model system for various fundamental and applied research in recent years. In total, the genomes of five different species have been sequenced over the past 10 y. ...Here, we report chromosome-scale reference genomes for five strawberry species, including three newly sequenced species' genomes, and genome resequencing data for 128 additional accessions to estimate the genetic diversity, structure, and demographic history of key
species. Our analyses obtained fully resolved and strongly supported phylogenies and divergence times for most diploid strawberry species. These analyses also uncovered a new diploid species (
Jia J. Lei). Finally, we constructed a pan-genome for
and examined the evolutionary dynamics of gene families. Notably, we identified multiple independent single base mutations of the
gene associated with white pigmented fruit shared by different strawberry species. These reference genomes and datasets, combined with our phylogenetic estimates, should serve as a powerful comparative genomic platform and resource for future studies in strawberry.
Postharvest decay is the leading constraint for extension of shelf life and marketing of strawberry fruit. In this work, strawberry fruit were treated with DENBA+ (0, control; 500 or 1200 V) and ...stored at 4 ℃ for 11 d to investigate its effect on postharvest quality. DENBA+ treated fruit showed reduced decay index, weight loss, firmness and pH value, compared to control. DENBA+ treatment had higher contents of soluble solids, total sugar, total phenol and anthocyanin, compared to control. In conclusion, DENBA+ could be considered an ecofriendly non-chemical alternative treatment for postharvest quality management of strawberry fruit.
•Impact of DENBA+ on the storage life of strawberry fruit was studied.•Decay index and weight loss were lower in treated strawberries compared to control.•DENBA+ treatment maintained total sugar, total phenol and total anthocyanin content.•The best results were obtained at voltage strength of 500 V.
•Bacillus halotolerans reduced the grey mould and induced defense enzymes in strawberry.•B. halotolerans KLBC XJ-5 harboured 6 antimicrobial biosynthesis gene clusters.•Four gene clusters of GH18 ...involved in chitin degradation were identified in KLBC XJ-5.•Fruit quality of strawberry is not impaired by B. halotolerans application.
Bacillus species are promising agent for the biological control of postharvest diseases. This study investigated the bio-control efficiency of Bacillus halotolerans KLBC XJ-5 against grey mould caused by Botrytis cinerea in postharvest strawberries, together with its underlying antagonistic mechanism. Treatment with B. halotolerans KLBC XJ-5 controlled mycelial growth as well as conidial germination of B. cinerea in vitro. The grey mould in strawberries inoculated with B. halotolerans KLBC XJ-5 was lower in comparison with that in the control fruit after 4 d of incubation. Genome sequencing and further bioinformatic analyses suggested that strain KLBC XJ-5 harboured six antimicrobial biosynthesis gene clusters, besides four glycoside hydrolase family 18 gene clusters involved in chitin degradation. In addition, it secreted the lytic enzyme chitinase (CHI). B. halotolerans KLBC XJ-5-treated strawberries exhibited significant induced enzyme activities (polyphenol oxidase, phenylalanine ammonia lyase, β-1, 3-glucanase, and chitinase) and compounds related to disease resistance (total phenols, flavonoids). Compared to the control fruit, B. halotolerans KLBC XJ-5-treated fruit did not present differences on nutritional quality (measured in ascorbic acid, titratable acidity, and total soluble solids). Thus, it can be concluded that B. halotolerans KLBC XJ-5 could be potentially useful as a suitable bio-control agent in harvested strawberries.
The protection and stability of flavours is the great importance in the food industry in order to produce high-quality products. For this reason, the main goal of this paper was.
to study the ...stability of a microencapsulated strawberry flavour using different encapsulating agents and drying techniques: spray drying, freeze drying and fluid bed. According to the quantification of volatile compounds, the blend MDs/Hi-Cap (9/1) at a fixed concentration of CDs (1.7%) was the most appropriate for microencapsulating the strawberry flavour. The best drying yield was observed in the case of freeze drying. In the case of the moisture content, spray drying samples presented the lowest values, followed by freeze drying and fluid bed. The study of stability at different temperatures and times revealed that the presence of CDs in the blend enabled a higher presence of volatile compounds in the powder than in its absence. Microphotographs showed smooth spherical particles in the case of spray drying, whereas the structure of the powder was amorphous, like glass, with freeze drying and irregular in the case of fluid bed.
Display omitted
•The freeze drying process provided the highest drying yield.•The spray drying technique produced a powder with the lowest moisture content.•The presence of CDs in the blend stabilized the volatile compounds during storage.•Spray drying samples presented typical spherical shape.•Spray drying was the best encapsulating technique for the strawberry flavour.
PDF
