Vlačno ispitivanje čelika za armiranje Košutić, Krešimir; Smolčić, Željko
Zbornik radova (Građevinski fakultet u Rijeci. Online),
11/2023, Letnik:
26, Številka:
1
Journal Article
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The paper describes in detail the tensile testing of steel for the reinforcement according to the HRN EN ISO 15630‐1:2010 norm. The aforementioned norm refers to the HRN EN ISO 6892‐1:2016 norm, ...which, due to the dependence of the mechanical properties of steel on the test speed, recommends method A that uses strain control. Tensile testing of steel for the reinforcement B500B was performed on three samples using method A1 of the same norm. The aim of the paper is to bring readers closer to the issue of ensuring the conditions for tensile testing of steel for the reinforcement with strain control and to provide guidelines for the application of the norm. The results of tensile test on steel for the reinforcement show that the Zwick Z 600E universal testing machine can provide strict tolerance limits on strain control rates.
U radu je detaljno opisano vlačno ispitivanje čelika za armiranje prema normi HRN EN ISO 15630‐1:2010. Navedena norma poziva se na normu HRN EN ISO 6892‐1:2016, koja zbog ovisnosti mehaničkih svojstava čelika o brzini nanošenja opterećenja preporučuje metodu A, koja koristi kontrolu brzine deformacije. Vlačno ispitivanje čelika za armiranje B500B je provedeno na tri uzorka primjenom metode A1 iste norme. Cilj rada je približiti čitateljima problematiku osiguranja uvjeta za vlačno ispitivanje čelika za armiranje kontrolom deformacije te dati smjernice za primjenu norme. Rezultati vlačnog ispitivanja čelika za armiranje pokazuju da kidalica Zwick Z 600E može osigurati stroge granice tolerancije na brzine deformacije.
A new series of 3-benzoylamino-5-imidazol-5-yl-benzobthiophenes and the parent amino derivatives were synthesized and screened as antitumor agents. All tested compounds showed concentration-dependent ...antiproliferative activity profile against HeLa cell line, exhibiting GI50 values in the low micromolar range. The most active compounds were tested in cell cycle perturbation experiments. A rapid accumulation of cells in the G2/M phase, with a concomitant reduction of cells in both the S and G0/G1 phases, was observed, suggesting that cell exposure to selected derivatives produces mitotic failure. To rationalize the biological results, the 3-benzoylamino-benzobthiophenes were analyzed through the in silico VLAK protocol. Compounds presenting the 3,4,5-trimethoxy-benzoyl moiety were in silico classified as potential antimitotic agents or topoisomerase II inhibitors, in good agreement with the biological studies.
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•A new series of 3-benzoylamino-5-imidazol-benzobthiophenes were synthesized.•All synthesized compounds showed antiproliferative activity against HeLa cell line.•Rapid accumulation of cells in the G2/M phase was observed.•The benzothiophenes were analyzed by the in silico VLAK protocol.
Príspevok stručne popisuje nehodovosť na priecestiach v správe ŽSR v rámci Slovenskej republiky pričom následná analýzy determinuje vzťah medzi počtom evidovaných vozidiel a počtom nehôd na ...priecestiach spolu s prijatým návrhom na ich celkovú elimináciu.
Drug design was performed through the Virtual Lock-and-Key (VLAK) protocol. This in silico approach allowed to select new annelated thienotriazolopyrimidine derivatives, potentially antitumor drugs. ...Starting from benzothieno2,3-e1,2,3triazolo1,5-apyrimidine and Pyrido3',2':4,5thieno2,3-e1,2,3triazolo1,5-apyrimidine core structures, new derivatives of these nuclei were designed and synthesized. Three of them were selected by the Development Therapeutical Program (DTP) of the National Cancer Institute (NCI) for the anticancer screening against a panel of 60 human tumor cell lines. The biological results showed that the new derivatives exhibited an excellent antiproliferative activity reaching sub-micromolar concentration. Moreover, to be evidenced their low toxicity and high potency.
The chemometric protocol VLAK was applied to predict improvement of the biological activity of pyrrolo-pyrimidine derivatives as anticancer agents, by using the NCI ACAM Database as depository of ...antitumor drugs with a known mechanism of action. Among the selected compounds two of these showed a good increase in the antitumor activity. These new pyrrolo-pyrimidine compounds were demonstrated effective against the full panels of NCI DTP tumour human cell lines. The derivative 8-3-(piperidino)propyl-4,10-dimethyl-9-phenyl-6-(methylsulfanyl)-3,4-dihydropyrimido1,2-cpyrrolo3,2-epyrimidin-2(8H)-one reveled efficacious against the leukemia subpanel, in particular the RPMI cell line resulted the most sensitive (pGI50 = 6.68). Moreover the derivative 7-(3-Chloropropyl)-9-methyl-5-(methylsulfanyl)-8-phenyl-3H-imidazo1,2-cpyrrolo3,2-epyrimidin-2(7H)-one showed a good antitumor activity against the leukemia subpanel with a low cytotoxic activity, above all against the HCT11 human tumour cell line.
The VLAK protocol revealed a good method to design new molecules with good antitumor activity, starting from low active compounds. Moreover this protocol focused on the pyrrolo-pyrimidine derivatives as useful starting point for further development to obtain more potent antitumor agents.
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► Lead optimization was performed through the Virtual Lock-and-Key (VLAK) protocol. ► The VLAK was applied in the design of pyrrolo-pyrimidines as anticancer agents. ► The NCI ACAM Database was used as database in the VLAK. ► A good improve of antitumor activity for new pyrrolo-pyrimidines was achieved. ► The VLAK revealed a good method to design new compounds with antitumor activity.
New benzothieno3,2-d-1,2,3-triazines, together with precursors triazenylbenzobthiophenes, were designed, synthesized and screened as anticancer agents. The structural features of these compounds ...prompted us to investigate their DNA binding capability through UV-vis absorption titrations, circular dichroism, and viscometry, pointing out the occurrence of groove-binding. The derivative 3-(4-methoxy-phenyl)benzothieno3,2-d-1,2,3-triazin-4(3H)-one showed the highest antiproliferative effect against HeLa cells and was also tested in cell cycle perturbation experiments. The obtained results assessed for the first time the anticancer activity of benzothieno3,2-d-1,2,3-triazine nucleus, and we related it to its DNA-binding properties.
In order to investigate their anticancer potential, four new pyrazolo1,2-abenzo1,2,3,4tetrazinone derivatives, designed through the chemometric protocol VLAK, and three of the most active compounds ...of the previous series have been evaluated on some cellular events including proliferation, apoptosis induction, and cell cycle. The NCI one dose (10 μM) screening revealed that the 8,9-di-methyl derivative showed activity against Leukemia (CCRF-CEM) and Colon cancer cell line (COLO 205), reaching 81% and 45% of growth inhibition (GI), respectively. Replacement of the two methyl groups with two chlorine atoms maintained the activity toward Leukemia cell (CCRF-CEM, GI 77%) and selectively enhanced the activity against COLO 205 attaining a LD50 in the μM range and against SW-620 a GI of 77%. Interestingly, an appreciable growth inhibition of 47% against therapeutically “refractory” Non-Small Cell Lung Cancer (NCI-H522) was observed. Moreover, the apoptosis induction, based on mitochondrial membrane depolarization, was found in the range EC50 3–5 μM on HeLa cell, evidencing a well defined relationship with the related in vitro cell growth inhibitory assays (MTT) performed against other selected tumor cell lines not included in the NCI tumor panel (HeLa, cervix; H292, lung; LAN-5, CNS; CaCo-2, colon; 16HBE, normal human cell lung) and against MCF-7 tumor cell line (breast). Only for the most active compounds, further cell cycle tests on HeLa displayed a cell arrest on S phase. Thus, a promising new class of anticancer candidates, acting as valuable apoptotic inductors, is proposed.
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•New class of apoptosis inductors acting in the μM range and blocking cell cycle in S phase.•Hit optimization was performed through the Virtual Lock-and-Key protocol (VLAK).•The best ranked derivative was also the most active in all the biological assays.
A new series of 3‐benzoylamino‐5‐(1H‐imidazol‐4‐yl)methylaminobenzobfurans were synthesized and screened as antitumor agents. As a general trend, tested compounds showed concentration‐dependent ...antiproliferative activity against HeLa and MCF‐7 cancer cell lines, exhibiting GI50 values in the low micromolar range. In most cases, insertion of a methyl substituent on the imidazole moiety improved the antiproliferative activity. Therefore, methyl‐imidazolyl‐benzobfurans compounds were tested in cell cycle perturbation experiments, producing cell cycle arrest with proapoptotic effects. Their core similarity to known colchicine binding site binders led us to further study the structure features as antitubulin agents by in silico protocols.
A new series of 3‐benzoylamino‐5‐(1H‐imidazol‐4‐yl)methylaminobenzobfurans were synthesized and screened as antitumor agents. The MTT test (HeLa and MCF‐7) underlined a significant growth inhibition effect of the derivatives 7a–f. Compounds 7b–d,f tested in cell cycle perturbation experiments on HeLa cells produced a cell cycle arrest in G2/M phase. In addition, the structure similarity of these benzobfurans with known colchicine binding sites binders led us to investigate their potent interference with the microtubule system by computational analysis as a support of the observed biological effects.
The work of science fiction author Odilius Vlak (Azua, Dominican Republic, 1976) looks to the future to comment on the country’s past. In many of the stories that comprise the collection Crónicas ...historiológicas (2017), Vlak examines how history is written and remembered through the eyes of future Dominicans. In this article, I examine Vlak’s counterfactual takes on the Dominican War of Independence and the Trujillo dictatorship in the stories “Descargas de meteoritos en la batalla del 19 de marzo” and “Juegodrox platónicos.” By injecting speculative elements into such significant moments in Dominican history, Vlak critiques how histories are created, sold, and mythologized, while also highlighting the role of counter-narratives in contesting official accounts. In “Descargas de meteoritos en la batalla del 19 de marzo,” a mysteriously powerful space rock wins the Battle of Azua, and future generations are confronted with a virtual record of the violence of the Dominican War of Independence. In “Juegodrox platónicos,” future Dominicans attempt to solve the mystery of disappearing children during the Trujillo era, while the dictator enlists science fiction writers and artists to fortify his larger-than-life persona. Examining Vlak’s challenging of Dominican history opens up the possibility of studying contemporary Caribbean science fiction’s relationship to the future and the past.
Článek popisuje jeden z analytických nástrojů aplikace sloužící k objednávání tras vlaků dopravci na území České republiky. Aplikace je součástí informačního systému sestavy ročního jízdního řádu ...vlaků. Jedná se o distribuovanou aplikaci složenou z databázového serveru, aplikačního serveru a klientského programu. Nástroj umožňuje výpočet řady ukazatelů pro potřeby nákladní i osobní dopravy. Výpočet lze provést pro vybrané tratě, oblasti, trakce a období. Oblasti mohou být standardní (Česká republika a její kraje) i uživatelem definované (libovolná množina dopravních bodů a úseků). Pomocí dalších parametrů lze ovlivnit výběr úseků tras vlaků, které budou do výpočtu zahrnuty. Po provedení výpočtu lze vyloučit nebo zpětně zařadit vybrané trasy vlaků, čímž dojde k okamžitému přepočtu výsledků.
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