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  • Identification and quantification of betulinic acid in different lamiaceae species using GC-MS
    Islamčević Razboršek, Maša ...
    Betulinic acid (3ß-hydroxy-Iup20(29)-en-28-oic acid) is a pentacyclic triterpenoid which has been identified in several plant tissues. Recently the compound has gained considerable interest because ... of its antibacterial, antiviral, anticancer and anti-inflammatory activities. Unfortunately, insufficient literature data is available concerning the distribution of betulinic acid in the Lamiaceae, because the published quantitative investigations are generally extended to only a few species, mainly from Betulaceae, Platanaceae and Ebenaceae. In presented study betulinic acid was successfully separated, identified and determined in different species of Lamiaceae family (rosemary-Rosmarinus officinalis L., sage-Salvia officinalisL., winter savory-Satureja montana, clary sage-Salvia sclarea and sticky sage-Salvia glutinosa), using gas chromatography and mass spectrometry (GC-MS). Compound has been identified as trimethylsilyl (TMS) derivative by retention time and mass spectral comparison with pure standard. The procedure involves ultrasonic extraction using solvent mixture of tetrahydrofuran and ethanol. Extracts were fractioned by size exclusion chromatography (SEC) after purifying on graphitized carbon. Fraction with betulinic acid was derivatised prior to GC-MS analysis using N-methyl-N-trimethylsilyl trifluoroacetamide (MSTFA) as a derivatisation reagent. Also, the studies were performed on fragmentation patterns of TMS derivative and characteristic fragments are discussed in the presented work. Quantitative analysis by GC-MS after silylating using cholesterol as an internal standard showed from 0.03 to 0.6 % of betulinic acid in air dried leaves of different Lamiaceae species. The lowest concentration was found in the leaves of Salvia glutinosa (<0.01 %).
    Vir: Food quality, an issue of molecula based science : proceedings (Vol. 2, str. 483-486)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2007
    Jezik - angleški
    COBISS.SI-ID - 11586070

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