E-viri
Recenzirano
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Golubev, Alexander M; Rojas, Adriana L; Nascimento, Alessandro S; Bleicher, Lucas; Kulminskaya, Anna A; Eneyskaya, Elena V; Polikarpov, Igor
Protein and peptide letters, 10/2008, Letnik: 15, Številka: 10Journal Article
Thermophilic endo-1,3(4)-beta glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method. The needle-like crystals belong to space group P2(1) and contain two protein molecules in the asymmetric unit with a solvent content of 51.75 %. Diffraction data were collected to a resolution of 1.95A and resulted in a dataset with an overall R(merge) of 10.4% and a completeness of 97.8%. Analysis of the structure factors revealed pseudomerohedral twinning of the crystals with a twin fraction of approximately 42%.
