Song, Dongyuan; Wang, Qingyang; Yan, Guanao; Liu, Tianyang; Sun, Tianyi; Li, Jingyi Jessica
Nature biotechnology,
02/2024, Letnik:
42, Številka:
2
Journal Article
We present a statistical simulator, scDesign3, to generate realistic single-cell and spatial omics data, including various cell states, experimental designs and feature modalities, by learning interpretable parameters from real data. Using a unified probabilistic model for single-cell and spatial omics data, scDesign3 infers biologically meaningful parameters; assesses the goodness-of-fit of inferred cell clusters, trajectories and spatial locations; and generates in silico negative and positive controls for benchmarking computational tools.
