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Zhang, Xue; Duan, Xue-Mei; Cheng, Jin; Qiao, Hong-Jiao; Dai, Yu-Mei
International journal of systematic and evolutionary microbiology 73, Številka: 12Journal Article
A bacterium, designated strain ZK17L-C2 , was isolated from the leaf tissues of wheat ( ) collected in Chengdu, Sichuan Province, PR China. It is aerobic, non-motile, Gram-negative, rod-shaped and red-to-pink in colour. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZK17L-C2 belonged to the genus and was most closely related to KCTC 12533 (98.68 %) and 9PBR-2 (98.19 %). Digital DNA-DNA hybridization (dDDH) values between strain ZK17L-C2 and these two type strains were 26.6 and 26.5 %, and average nucleotide identity (ANI) values were 84.9 and 84.8 %, respectively; these values are lower than the proposed and generally accepted species boundaries for dDDH and ANI. The genomic DNA G+C content of strain ZK17L-C2 was 59.4 mol%. It can grow at pH 5.5-7.5 and 15-30 °C, which is different from the closely related type strains. The major fatty acids of strain ZK17L-C2 were iso-C , C and C . Overall, the results from biochemical, chemical taxonomy and phylogenetic analyses indicate that strain ZK17L-C2 (=CGMCC 1.19373 =KCTC 92184 ) represents a new species of the genus , for which the name sp. nov. is proposed.
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