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Elliott, David A; Braam, Stefan R; Koutsis, Katerina; Ng, Elizabeth S; Jenny, Robert; Lagerqvist, Ebba L; Biben, Christine; Hatzistavrou, Tanya; Hirst, Claire E; Yu, Qing C; Skelton, Rhys J P; Ward-van Oostwaard, Dorien; Lim, Sue Mei; Khammy, Ouda; Li, Xueling; Hawes, Susan M; Davis, Richard P; Goulburn, Adam L; Passier, Robert; Prall, Owen W J; Haynes, John M; Pouton, Colin W; Kaye, David M; Mummery, Christine L; Elefanty, Andrew G; Stanley, Edouard G
Nature methods, 2011-Oct-23, Letnik: 8, Številka: 12Journal Article
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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